Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Castanea dentata
Species name
American chestnut (Castanea dentata) is a species of eudicot in the family Fagaceae (beech family).
Species info
Gene sequence info
Castanea_dentata.HI4_OMT.fa
Download
>Caden.03G096400.1.v1.1
MDFIHGEGMSELFQVQCHLYKHIFSYIDSMSLKCAIQLGIPDTIHNHGQPITLQELVSKLNIHPKKTSCVHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSRLILKDNVTSLSPFVLAMLDPALVSPWQFLGDWFQGSELTPFEKAHGKGFWDYCNQNPEYNNIFNEAMASDSRLMNLVVKDYKPIFEGLGSLVDVGGGTGTVARIISEAFPQMKCTVFDLPHVVANLPDSKNLKFAGGDMFQYIPPADAILFKWILHDWSDEECVSILKNCKEAITSKGKEGKVIVIDVVINQEKDEHDVTTTKLLFDALMMVLFTGKERNKKEWEKLFLEAGFSYYKIVSSFGMKSVIEIYP
>Caden.03G096500.1.v1.1
MDLIHGEGVSELFQVQCQLFKHIFSYIDCMSLKCAIQLGIPDIIHNHGQPITLPELVSKLHIHPKKTSCVHRLMRLLVHSGFFTKTIVHENQEKVKEEEAYTLTPSSRLILKDNVTSFSPYVLAMLDPVLLSPWQFLGDWFQGSELTPFEKTHGKGFWDHCNQNPEFNNNFNEAMASDSLLMNLVIKDYKPIFEGLGSLVDVGGGTGTMARIISETFPHMKCTVLDLPHVVANLPDSKNLKYVGGDMFQYIPPADAILFKWILHNWSDEECVNVLKNCKEAIMGKGKEGKVIIIDIMINEEKDEHDVTTAKLLLDALMMVLLTGKERNQKEWEKLFLEAGFSHYKIVSSFGMKSLIEIYP
>Caden.03G096100.1.v1.1
MDLIHGEGVSELFQVQCHLYKHIFSYIDSMSLKCAIQLGIPDAIHNHGQPITLQELVSKLNIHPKKTSCVHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSMLILKDSATSLSPFVLAILDPALVSPWQFLGDWFQGSELTPFEQAHGKGFWDYCNQSPEYNNIFNEGMASDSRLMSLVVKDYQPIFEGLGSLVDVGGGTGTVARIISEAFPHMKCTVFDLPHVVADLPDSKNLKFVGGDMFQYIPPADAILFKCILHDWSDEECVNILKNCKEATAKKGKKER
>Caden.03G096300.1.v1.1
MDLIHGEGVSELFQVQCHLYKHIFSYIDSMSLKCAIQLGIPDTIYNHGQPITLQELVSKLNIHPKKTSCVHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSRLILKDNVTSLSPFVLAMLDPALVSPWQFLGDWFQGSELTPFEKAHGKGFWDYCNQNPEYNNIFNEAMASDSRLMNLVVKDYKPIFEGLGSLVDVGGGTGTVARIISEEFPHMKCTVFDLPHVVANLPDGKNLKFVGGDMFQYVPPADAVLFKWILHDWSDEECVKILKNCKEAITSKGKEGKVIIIDVVINQEKDEHDVTTTKLLFDALMMVLLTGKERNKKEWEKLFLEAGFSHYKIVSSFGMKSVIEIYP
>Caden.03G096200.1.v1.1
MDLIHDEGVSKLFQVQCHLYKHIFGYIDSMSLKCAIQLGIPDTIHNHGQPITLQELVSKLHIHPKKTSCLHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSRLILKDNVTSLSPFVLAMLDPALVSPWQFLGDWFRGSELTPFEKAHGKGAWDYCNQNPEYNNIFNEGMASDSRLMNLVVKDYKPIFEGLGSLVDVGGGTGTVARIISEAFPHLKCTVFDLPHVVANLPDSKNLKFVAGDMFQYIPPADAVLFKWILHDWSDEECVKILKNCKEAITSKGKEGKVIIIDVVINQEKDEHDVTTTKLLFDALMMALVTGKERNKKEWEKLFLEAGFSHYKIVSSFGMKSVIEIYP
>Caden.05G131700.1.v1.1
MDLVHGQRASELFQAQSHLYKHIFNFIGSMSLKCALQLGIPEIIHNHGKPITLPELISALQIHPTKAGFVHRLMRLLVHSGFFVTTRVHINQEEEEEEAYDLTPSSRILLKDNVTNLSPFLLAMLDPVLVSPWHFLESWFRGDKVTPFESANGMGFWDYADQNPDFNDSFNKAMASDSGMMNLVVKDCKPVFEGLDTLVDVGGGTGTCARIISEAFPHLKCTVLELPHVVANLADNSNLNFVGGDMFQSIPSADAILLKLVLHALNDEECVKVLKKCREAISNKGKEGKVIIIDIVIDEKKDQHEITSAKLFFDMLMMVVVTGRERHEKELEKLFLEAGFSHYKIKPLFGLRSLIEVYP
>Caden.05G131500.1.v1.1
MSLKCALQLGIPEIIHNHGKPITLPELISALQIHPTKAGFVHRLMRLLVHSGFFVTTRVHINQQEEEEEEAYDLTPSSRILLKDNVTNLSPFLLAMLDPVLVSPWHFLESWFRGDKVTPFESANGMGFWDYADQNPDFNDSFNKAMASDSGMMNLVVKDCKPVFEGLDTLVDVGGGTGTCARIISEAFPHLKCTVLELPHVVANLADNSNLNFVGGDMFQSIPSADAILLKVK
>Caden.07G193700.1.v1.1
MVKGSQWYISLFLFCNSSFFICIYIYRNLQPEGLNTAISKRLLKLREMEKTQREAKWNEEEQAEVDIWKYILGFTEMAVVRCAIELGIADAIESHGSPMTLSELSSTLGCDPSPLYRIMRFLMHRGIFKGTLNSQGSPGYAQTPLSRRLMKHGEHSMAALILLESSPVLLTPWLSLSARVLDDDTSSFEVAHGEDIWSYSAANPAHGQLINEAMACDARVLVPAMIHGCPEVFDGLGSLVDVGGGNGTTLKVLIKAFPWLRGINFDQPHVVSVAGVISGVENVGGDMFESVPKADAAFLKWILHDWGDNECIQILKKCREAVLENKGKVIIVDAVIEEAEIDKLTDVRLALDMAMMAHTNKGKERTLKEWGFVLGEAGFSRYTVKTIHAVQSVIEAFP
>Caden.07G193900.1.v1.1
MEKTQREEKWNEEEQAEVDIWKYIFGFTEMAVVKCAIDLGIADAIESHGSPMTLSELSSSLGCAPSPLYRIMRFLMHRGIFKGKLTSQGSPGYAQTPLSRRLMKHGENSMAALILLESSPVMLTPWLSLSARVLADGTSSFEVAHGEDIWSYSASNPAHGQLINEAMACDARVIVPAMIHGCPEVFDGLSSLVDVGGGNGETLKVLVKAFPWLRGINFDQPHVVSVAGVISGVENVGGDMFESVPKADAAFLKWVLHDWGDNECIQILKKCRQAVLENKGKVIIVDAVIEEAEKDKLTDVKLALDMAMMAHTNKGKERTLKEWGSVIGEAGFSRYTVKTIHAVQSVIEAFP
>Caden.M010900.1.v1.1
MDLVRGQRASELFQAQSHLYKHIFNFIGSMSLKCALQLGIPEIIHNHGKPITLPELISALQIHPTKAGFVHRLMRLLVHSGFFVTTRVHINQQEEEEEEAYDLTPSSRILLKDNVTNLSPFLLAMLDPVLVSPWHFLESWFRGDKVTPFESANGMGFWD
MDFIHGEGMSELFQVQCHLYKHIFSYIDSMSLKCAIQLGIPDTIHNHGQPITLQELVSKLNIHPKKTSCVHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSRLILKDNVTSLSPFVLAMLDPALVSPWQFLGDWFQGSELTPFEKAHGKGFWDYCNQNPEYNNIFNEAMASDSRLMNLVVKDYKPIFEGLGSLVDVGGGTGTVARIISEAFPQMKCTVFDLPHVVANLPDSKNLKFAGGDMFQYIPPADAILFKWILHDWSDEECVSILKNCKEAITSKGKEGKVIVIDVVINQEKDEHDVTTTKLLFDALMMVLFTGKERNKKEWEKLFLEAGFSYYKIVSSFGMKSVIEIYP
>Caden.03G096500.1.v1.1
MDLIHGEGVSELFQVQCQLFKHIFSYIDCMSLKCAIQLGIPDIIHNHGQPITLPELVSKLHIHPKKTSCVHRLMRLLVHSGFFTKTIVHENQEKVKEEEAYTLTPSSRLILKDNVTSFSPYVLAMLDPVLLSPWQFLGDWFQGSELTPFEKTHGKGFWDHCNQNPEFNNNFNEAMASDSLLMNLVIKDYKPIFEGLGSLVDVGGGTGTMARIISETFPHMKCTVLDLPHVVANLPDSKNLKYVGGDMFQYIPPADAILFKWILHNWSDEECVNVLKNCKEAIMGKGKEGKVIIIDIMINEEKDEHDVTTAKLLLDALMMVLLTGKERNQKEWEKLFLEAGFSHYKIVSSFGMKSLIEIYP
>Caden.03G096100.1.v1.1
MDLIHGEGVSELFQVQCHLYKHIFSYIDSMSLKCAIQLGIPDAIHNHGQPITLQELVSKLNIHPKKTSCVHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSMLILKDSATSLSPFVLAILDPALVSPWQFLGDWFQGSELTPFEQAHGKGFWDYCNQSPEYNNIFNEGMASDSRLMSLVVKDYQPIFEGLGSLVDVGGGTGTVARIISEAFPHMKCTVFDLPHVVADLPDSKNLKFVGGDMFQYIPPADAILFKCILHDWSDEECVNILKNCKEATAKKGKKER
>Caden.03G096300.1.v1.1
MDLIHGEGVSELFQVQCHLYKHIFSYIDSMSLKCAIQLGIPDTIYNHGQPITLQELVSKLNIHPKKTSCVHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSRLILKDNVTSLSPFVLAMLDPALVSPWQFLGDWFQGSELTPFEKAHGKGFWDYCNQNPEYNNIFNEAMASDSRLMNLVVKDYKPIFEGLGSLVDVGGGTGTVARIISEEFPHMKCTVFDLPHVVANLPDGKNLKFVGGDMFQYVPPADAVLFKWILHDWSDEECVKILKNCKEAITSKGKEGKVIIIDVVINQEKDEHDVTTTKLLFDALMMVLLTGKERNKKEWEKLFLEAGFSHYKIVSSFGMKSVIEIYP
>Caden.03G096200.1.v1.1
MDLIHDEGVSKLFQVQCHLYKHIFGYIDSMSLKCAIQLGIPDTIHNHGQPITLQELVSKLHIHPKKTSCLHRLMRLLVHSGFFAKTIVHENQEKEKEEEAYTLTPSSRLILKDNVTSLSPFVLAMLDPALVSPWQFLGDWFRGSELTPFEKAHGKGAWDYCNQNPEYNNIFNEGMASDSRLMNLVVKDYKPIFEGLGSLVDVGGGTGTVARIISEAFPHLKCTVFDLPHVVANLPDSKNLKFVAGDMFQYIPPADAVLFKWILHDWSDEECVKILKNCKEAITSKGKEGKVIIIDVVINQEKDEHDVTTTKLLFDALMMALVTGKERNKKEWEKLFLEAGFSHYKIVSSFGMKSVIEIYP
>Caden.05G131700.1.v1.1
MDLVHGQRASELFQAQSHLYKHIFNFIGSMSLKCALQLGIPEIIHNHGKPITLPELISALQIHPTKAGFVHRLMRLLVHSGFFVTTRVHINQEEEEEEAYDLTPSSRILLKDNVTNLSPFLLAMLDPVLVSPWHFLESWFRGDKVTPFESANGMGFWDYADQNPDFNDSFNKAMASDSGMMNLVVKDCKPVFEGLDTLVDVGGGTGTCARIISEAFPHLKCTVLELPHVVANLADNSNLNFVGGDMFQSIPSADAILLKLVLHALNDEECVKVLKKCREAISNKGKEGKVIIIDIVIDEKKDQHEITSAKLFFDMLMMVVVTGRERHEKELEKLFLEAGFSHYKIKPLFGLRSLIEVYP
>Caden.05G131500.1.v1.1
MSLKCALQLGIPEIIHNHGKPITLPELISALQIHPTKAGFVHRLMRLLVHSGFFVTTRVHINQQEEEEEEAYDLTPSSRILLKDNVTNLSPFLLAMLDPVLVSPWHFLESWFRGDKVTPFESANGMGFWDYADQNPDFNDSFNKAMASDSGMMNLVVKDCKPVFEGLDTLVDVGGGTGTCARIISEAFPHLKCTVLELPHVVANLADNSNLNFVGGDMFQSIPSADAILLKVK
>Caden.07G193700.1.v1.1
MVKGSQWYISLFLFCNSSFFICIYIYRNLQPEGLNTAISKRLLKLREMEKTQREAKWNEEEQAEVDIWKYILGFTEMAVVRCAIELGIADAIESHGSPMTLSELSSTLGCDPSPLYRIMRFLMHRGIFKGTLNSQGSPGYAQTPLSRRLMKHGEHSMAALILLESSPVLLTPWLSLSARVLDDDTSSFEVAHGEDIWSYSAANPAHGQLINEAMACDARVLVPAMIHGCPEVFDGLGSLVDVGGGNGTTLKVLIKAFPWLRGINFDQPHVVSVAGVISGVENVGGDMFESVPKADAAFLKWILHDWGDNECIQILKKCREAVLENKGKVIIVDAVIEEAEIDKLTDVRLALDMAMMAHTNKGKERTLKEWGFVLGEAGFSRYTVKTIHAVQSVIEAFP
>Caden.07G193900.1.v1.1
MEKTQREEKWNEEEQAEVDIWKYIFGFTEMAVVKCAIDLGIADAIESHGSPMTLSELSSSLGCAPSPLYRIMRFLMHRGIFKGKLTSQGSPGYAQTPLSRRLMKHGENSMAALILLESSPVMLTPWLSLSARVLADGTSSFEVAHGEDIWSYSASNPAHGQLINEAMACDARVIVPAMIHGCPEVFDGLSSLVDVGGGNGETLKVLVKAFPWLRGINFDQPHVVSVAGVISGVENVGGDMFESVPKADAAFLKWVLHDWGDNECIQILKKCRQAVLENKGKVIIVDAVIEEAEKDKLTDVKLALDMAMMAHTNKGKERTLKEWGSVIGEAGFSRYTVKTIHAVQSVIEAFP
>Caden.M010900.1.v1.1
MDLVRGQRASELFQAQSHLYKHIFNFIGSMSLKCALQLGIPEIIHNHGKPITLPELISALQIHPTKAGFVHRLMRLLVHSGFFVTTRVHINQQEEEEEEAYDLTPSSRILLKDNVTNLSPFLLAMLDPVLVSPWHFLESWFRGDKVTPFESANGMGFWD
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University