Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Carthamus tinctorius
Species name
Safflower (Carthamus tinctorius) is a species of eudicot in the family Asteraceae (daisy family).
Species info
Gene sequence info
Carthamus_tinctorius.ROMT.fa
Download
>CtAH04T0206700.1
MALQNGELSSDLLHSQAHIWNHIFSFINSMSLKCAIQLHIPDIIHRHGAPMLLSELVEALSINKERSHFVYRLMRILVHSGFFLKQRISISEGDGEGEEKEVYLLAPASRLLLKEEPLSMRPFLLAMLDPILVDPWQHMSKWLQNDEDICPFHTAHGRTFWDFAGQDPKLNRFFNEAMASDARLVTNVILKHSWGVFEGINSIVDVGGGTGIVAKSIAKVFPKLNCISFDLPHVVDGMEGSENLSYVGGDMFDAIPKADAVLMKWILHDWSDEECIKILKRCKEAIPSKKNGGKLMIIDMVVMKSHEEGDNSLETQFFFDMLMMALATGKERTEKEWKKLLVDDAGFSNYKITPILGLRSLIEVYP
>CtAH04T0207300.1
MALQNGELSSYLLHSQAHIWNHIFSFINSMSLKYAIQLQIPDIIHRHGAPMLCSELVEALSINKERSHFVYRLMRILVHSGFFLKQRISINEGDEVEEKEAYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDMSRWFQNDDDASCPFQTTYGKSLWDFAGQEPKLNHFFNEAMASDARLVTNVVLKHYKGAFEGLDSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKWILHDWNDEKCIKILKRCKEAIPSKENRGKLIIIDMVVMNNEVGSDESLETQLFFDMLMMTLTTGTERTEKEWVKLLLDAGFSDYKFTPILGLRSLIEKIEMALQNSQLSNDLLHSQAHIWNHIFSFINSMSLKCAIQLQIPDIIHRHGEPMLLSELVEALSINKERSHFVYRLMRILIHSGFFLKQKIFITEGEEEREEENEVYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDISRWFQNDDDAACPFQTTYGKSLWDFAGQEPKLNHFFNEAMASDARLVTNVVLKNYKGAFEGLDSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADVVFVKWILHDWSDEECIKILKRCKEAIPSKENRGKLIIIDMVVTNNEVGSDESLETQLFFDMLMMTVTTGKERTEKEWIKLLLDAGFSEYKFTPILGLRSLIEKIEMALHNGELPSHLLHSQAHIWNHIFSFINSMSLCAIQLQIPDIIHRHGAPMLCSELVEALSINKERSHFIYRLMRILVHSGFFLKQRISINEGDEVEEKEAYLLAPVFLVSF
>CtAH04T0207000.1
MSLKCAIQLQIPDIIHRLGILMLLSELVEVLSINKEISHFVYRLMRILVHSGDGEREGKEVYLLAPASRFLLKEESLRIRPYLLAMLDPILVDRRQHMSKWLQNDEDICIFHTARGGKHFGMSQAKIQNLTGSSMKQWLVMQDLLQMSFLNTVEVILKV
>CtAH04T0206900.1
MALQNGELSNDLLHSQAHIWNHIFSFINSMSLKCAIQLQIPDIIHRHGAPMLLSELVEALSINKERSHFVYRLMRILVHSGFFLKQRTSISEGDGEGEEKEAYLLAPASRLLLKEEPLNMRPFLLAMLDPILVDPWQHMSKWLQNDEDICPFHTAHGRTFWDFAGEDPKLNRFFNEAMASDARLVTNVILKHSRGVFEGINSIVDVGGGTGIVAKSIAKVFPKLNCISFDLPHVVNGMEGSENLSYVGGDMFEAIPKADAVLVKWILHDWSDEECIKILKRCKEAIPSKKNGGKLMIIDMVVMKNREEGDNSLETQFFFDMLMMALTTGRERTEKEWKKLLVDDAGFSNYKITPILGLRSLIEMALQNGELSNDVLRSQAHIWNHIFSFVKSMSLKCAIQLQIPDVIHRHGAPMLLSELVEALAINKERSHFVYRLMRILVHSGFFLKQRLSVSEGDRKGEGKEVYLLAPASRLLLKEEPLSIRPFLLANLDPILIDPWQYMSKWLQNDDDIYPFHTAHGRTFWDFAGQDPKLNRFFNEAMASDARLVTDIILKHSRGAFEGINSIVDVGGGTGKVSKSISKAFPQLNCISFDLPHVVNGMEGSKNLSYVGGDMFVAIPKADAVLMKWILHDWSDEECIKILKRCKEAIPCKENGGKVMIVDMVVMNEEGDTSLETQLFFDMLMMTLTTGKERTEKEWKKLLVDDAGFNDYKITPILGLRSLIEVK
>CtAH04T0207400.1
MSFLSTTRVLLKVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKQGKWRRKSNYHEVGSNESLKPPLFFDMLMMNVTTGKERTEKAWKLEMALQNGQLSSDLLHSQAHIWNHIFSFINSMSLKCAIQLQIPDIIHRHGAPMRLSELVEALSINKERSHFVYRLMRILVHSGFFLKQKMTVTEGEVEEEEENEAYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDMSKWFQNDDRDGVCPFQTTHGRSLWDFAGQEPKLNQFFNEAMASDARLVTNVVLKNYKGAFEGLDSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKWILHDWNDEECIKILKRCKEAIPSKENGGKLIIVDMVVKGHEVGPDKSLKTQLFFDMLMMTLVTGKERTEKDWGKSIQVEVKARIVDVFLYDAISRASIEESLIREVVVVECVWRDQIEWCQVSVPSAIGGVRAVGPE
>CtAH04T0207100.1
MIVDMVVMNEEGDNSLKTQLFFDMLMMTLLTGREMTEKEWKKLLLDDVGFNDYKITPILRLRSLLEVYP
>CtAH04T0206600.1
MVLEATPSQLVYHITKKEMALQYGDICNDMLHSHAQIWNHIFSFISSMSLKCAIQLQIPDIIDRHGAPMLLSELVDALSINKQRSHSVYRLMRILVHSGFFMKQTISVTEGDKEEEREGYLLTPTSHFLLKEGPFSLRPFLLVVLDPILMDPWQHMSKWFQNDDICPFHTTHGRTFWDFAGQDPKLSQLFNEAMASDARLVANVILKQCRGAFEGLNSIADVGGGTGTVAKAIAEAFPKLNCICFDLPHVVNGMEGNNNFSYVGGDMFEVIPKVDAILLKWILHDWSDDECIKILKRCKEAIPSKENGGKIIIIDMVVMKNHEEGDDSLETQLCFDMLMMTLATGRERTEKDWEKLLIDDVGFSNYKITPILGLRSLIEVYP
>CtAH04T0207200.1
MKTRIFPRQLLLSKRLSDKVSKVYTIKQFTTCIDYKKIEMALQNDSMSLKCAIQLQIPDIIQRHGAPMLLSELVEALSINKERSHFVYRLMRILVHSGFFLKQKITVNEGEVEQEEENEAYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDMSKWFQIDDHDDACPFQTTHGRNLWDFAGQEPKLNQFFNEAMASDATLVTNVVLKHYKGVFEGLSSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKRCKEAIPSKENGGKLIIVDMVVKSHEVGSDESLKTQLFFDMLMMTLVTGKERTKKDWGYFCHLILFH
>CtAH06T0148700.1
MRISALFIQRMEERIGILQAQDPNLNDFFNEAMASLNSIVDVGGGTGTFAKAIAEAFPTHNCIIFDLPHVVNGMEGRNNFSYVRGDVFEAIPKADAMLKRCKEAIPQKENGGKLVIIDMVMKNHDGGDDSLRTQLFFDMLLILKT
>CtAH06T0149000.1
MALQNDELSLDMLRSQAHIWNHMFSFINSMSLKCAVQLQIPDIIHRHGAPMMLSELVEALSINKERSRFVYRLMRILVYSGFFQTQTISITKGDEENETEGYLLTPTSRFLLKEEPLSIRPFLLAMLDPIFMDPWQDMSRWFQNEDICPFYTTHGRTYWDFASQDPNLNDFFNEAMASDARLVTNVILKHCNSAFEGLNSIVDVGGGTGIFAKAIAEAFPTLNCISFDLPHVVNGMEGSNNLSYVGGDMFEAIPKADAVLMKWMLHAWSDEECIKILKRCKEAIPKKENGGKLMIIDMVVKNHEEDDDSLRTQLFFDMLLMILTTGREKSEKDWKKLLLDDAGFSDYKIIPILGLRSFIEVYP
>CtAH06T0148600.1
MALQNDELSVDMLRSQAHIWNHMFSFINSMSLKCAIQLQIPDIIHRHGAPMMLSELVEAFSIDKERSCFIYRLMRILVHSGYFQMQTISIGEGEKENGRKGNLLTPTSRFLLKEEPLSIRPFSLAMLDPIFMDPRQDMRRWFQNEDICPFYTTHGRTYWDFVGQDPNLDDFFNEVMAIDARLVTNVILKHCNGAFEGLNSIVDVGGGTATFGKASSRCQWYGRKLKYSYVGEDMFEAIPKADAILMKMQRSNSKKGKWRKINDHIHGSEEGDDSLTTQLFYDMLLMILTTGREKSEKDWKKLSLDDVGFSDYKIIPILGLRSLIKVYPV
MALQNGELSSDLLHSQAHIWNHIFSFINSMSLKCAIQLHIPDIIHRHGAPMLLSELVEALSINKERSHFVYRLMRILVHSGFFLKQRISISEGDGEGEEKEVYLLAPASRLLLKEEPLSMRPFLLAMLDPILVDPWQHMSKWLQNDEDICPFHTAHGRTFWDFAGQDPKLNRFFNEAMASDARLVTNVILKHSWGVFEGINSIVDVGGGTGIVAKSIAKVFPKLNCISFDLPHVVDGMEGSENLSYVGGDMFDAIPKADAVLMKWILHDWSDEECIKILKRCKEAIPSKKNGGKLMIIDMVVMKSHEEGDNSLETQFFFDMLMMALATGKERTEKEWKKLLVDDAGFSNYKITPILGLRSLIEVYP
>CtAH04T0207300.1
MALQNGELSSYLLHSQAHIWNHIFSFINSMSLKYAIQLQIPDIIHRHGAPMLCSELVEALSINKERSHFVYRLMRILVHSGFFLKQRISINEGDEVEEKEAYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDMSRWFQNDDDASCPFQTTYGKSLWDFAGQEPKLNHFFNEAMASDARLVTNVVLKHYKGAFEGLDSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKWILHDWNDEKCIKILKRCKEAIPSKENRGKLIIIDMVVMNNEVGSDESLETQLFFDMLMMTLTTGTERTEKEWVKLLLDAGFSDYKFTPILGLRSLIEKIEMALQNSQLSNDLLHSQAHIWNHIFSFINSMSLKCAIQLQIPDIIHRHGEPMLLSELVEALSINKERSHFVYRLMRILIHSGFFLKQKIFITEGEEEREEENEVYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDISRWFQNDDDAACPFQTTYGKSLWDFAGQEPKLNHFFNEAMASDARLVTNVVLKNYKGAFEGLDSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADVVFVKWILHDWSDEECIKILKRCKEAIPSKENRGKLIIIDMVVTNNEVGSDESLETQLFFDMLMMTVTTGKERTEKEWIKLLLDAGFSEYKFTPILGLRSLIEKIEMALHNGELPSHLLHSQAHIWNHIFSFINSMSLCAIQLQIPDIIHRHGAPMLCSELVEALSINKERSHFIYRLMRILVHSGFFLKQRISINEGDEVEEKEAYLLAPVFLVSF
>CtAH04T0207000.1
MSLKCAIQLQIPDIIHRLGILMLLSELVEVLSINKEISHFVYRLMRILVHSGDGEREGKEVYLLAPASRFLLKEESLRIRPYLLAMLDPILVDRRQHMSKWLQNDEDICIFHTARGGKHFGMSQAKIQNLTGSSMKQWLVMQDLLQMSFLNTVEVILKV
>CtAH04T0206900.1
MALQNGELSNDLLHSQAHIWNHIFSFINSMSLKCAIQLQIPDIIHRHGAPMLLSELVEALSINKERSHFVYRLMRILVHSGFFLKQRTSISEGDGEGEEKEAYLLAPASRLLLKEEPLNMRPFLLAMLDPILVDPWQHMSKWLQNDEDICPFHTAHGRTFWDFAGEDPKLNRFFNEAMASDARLVTNVILKHSRGVFEGINSIVDVGGGTGIVAKSIAKVFPKLNCISFDLPHVVNGMEGSENLSYVGGDMFEAIPKADAVLVKWILHDWSDEECIKILKRCKEAIPSKKNGGKLMIIDMVVMKNREEGDNSLETQFFFDMLMMALTTGRERTEKEWKKLLVDDAGFSNYKITPILGLRSLIEMALQNGELSNDVLRSQAHIWNHIFSFVKSMSLKCAIQLQIPDVIHRHGAPMLLSELVEALAINKERSHFVYRLMRILVHSGFFLKQRLSVSEGDRKGEGKEVYLLAPASRLLLKEEPLSIRPFLLANLDPILIDPWQYMSKWLQNDDDIYPFHTAHGRTFWDFAGQDPKLNRFFNEAMASDARLVTDIILKHSRGAFEGINSIVDVGGGTGKVSKSISKAFPQLNCISFDLPHVVNGMEGSKNLSYVGGDMFVAIPKADAVLMKWILHDWSDEECIKILKRCKEAIPCKENGGKVMIVDMVVMNEEGDTSLETQLFFDMLMMTLTTGKERTEKEWKKLLVDDAGFNDYKITPILGLRSLIEVK
>CtAH04T0207400.1
MSFLSTTRVLLKVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKQGKWRRKSNYHEVGSNESLKPPLFFDMLMMNVTTGKERTEKAWKLEMALQNGQLSSDLLHSQAHIWNHIFSFINSMSLKCAIQLQIPDIIHRHGAPMRLSELVEALSINKERSHFVYRLMRILVHSGFFLKQKMTVTEGEVEEEEENEAYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDMSKWFQNDDRDGVCPFQTTHGRSLWDFAGQEPKLNQFFNEAMASDARLVTNVVLKNYKGAFEGLDSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKWILHDWNDEECIKILKRCKEAIPSKENGGKLIIVDMVVKGHEVGPDKSLKTQLFFDMLMMTLVTGKERTEKDWGKSIQVEVKARIVDVFLYDAISRASIEESLIREVVVVECVWRDQIEWCQVSVPSAIGGVRAVGPE
>CtAH04T0207100.1
MIVDMVVMNEEGDNSLKTQLFFDMLMMTLLTGREMTEKEWKKLLLDDVGFNDYKITPILRLRSLLEVYP
>CtAH04T0206600.1
MVLEATPSQLVYHITKKEMALQYGDICNDMLHSHAQIWNHIFSFISSMSLKCAIQLQIPDIIDRHGAPMLLSELVDALSINKQRSHSVYRLMRILVHSGFFMKQTISVTEGDKEEEREGYLLTPTSHFLLKEGPFSLRPFLLVVLDPILMDPWQHMSKWFQNDDICPFHTTHGRTFWDFAGQDPKLSQLFNEAMASDARLVANVILKQCRGAFEGLNSIADVGGGTGTVAKAIAEAFPKLNCICFDLPHVVNGMEGNNNFSYVGGDMFEVIPKVDAILLKWILHDWSDDECIKILKRCKEAIPSKENGGKIIIIDMVVMKNHEEGDDSLETQLCFDMLMMTLATGRERTEKDWEKLLIDDVGFSNYKITPILGLRSLIEVYP
>CtAH04T0207200.1
MKTRIFPRQLLLSKRLSDKVSKVYTIKQFTTCIDYKKIEMALQNDSMSLKCAIQLQIPDIIQRHGAPMLLSELVEALSINKERSHFVYRLMRILVHSGFFLKQKITVNEGEVEQEEENEAYLLAPASRLLLKEEPLSIRPFLLAMLDPILMDPWQDMSKWFQIDDHDDACPFQTTHGRNLWDFAGQEPKLNQFFNEAMASDATLVTNVVLKHYKGVFEGLSSIVDVGGGTGTVAKAIAKAFPKLNCISFDLPHVVNGMEGSKNLSYVGGDMFEAIPKADAVFVKRCKEAIPSKENGGKLIIVDMVVKSHEVGSDESLKTQLFFDMLMMTLVTGKERTKKDWGYFCHLILFH
>CtAH06T0148700.1
MRISALFIQRMEERIGILQAQDPNLNDFFNEAMASLNSIVDVGGGTGTFAKAIAEAFPTHNCIIFDLPHVVNGMEGRNNFSYVRGDVFEAIPKADAMLKRCKEAIPQKENGGKLVIIDMVMKNHDGGDDSLRTQLFFDMLLILKT
>CtAH06T0149000.1
MALQNDELSLDMLRSQAHIWNHMFSFINSMSLKCAVQLQIPDIIHRHGAPMMLSELVEALSINKERSRFVYRLMRILVYSGFFQTQTISITKGDEENETEGYLLTPTSRFLLKEEPLSIRPFLLAMLDPIFMDPWQDMSRWFQNEDICPFYTTHGRTYWDFASQDPNLNDFFNEAMASDARLVTNVILKHCNSAFEGLNSIVDVGGGTGIFAKAIAEAFPTLNCISFDLPHVVNGMEGSNNLSYVGGDMFEAIPKADAVLMKWMLHAWSDEECIKILKRCKEAIPKKENGGKLMIIDMVVKNHEEDDDSLRTQLFFDMLLMILTTGREKSEKDWKKLLLDDAGFSDYKIIPILGLRSFIEVYP
>CtAH06T0148600.1
MALQNDELSVDMLRSQAHIWNHMFSFINSMSLKCAIQLQIPDIIHRHGAPMMLSELVEAFSIDKERSCFIYRLMRILVHSGYFQMQTISIGEGEKENGRKGNLLTPTSRFLLKEEPLSIRPFSLAMLDPIFMDPRQDMRRWFQNEDICPFYTTHGRTYWDFVGQDPNLDDFFNEVMAIDARLVTNVILKHCNGAFEGLNSIVDVGGGTATFGKASSRCQWYGRKLKYSYVGEDMFEAIPKADAILMKMQRSNSKKGKWRKINDHIHGSEEGDDSLTTQLFYDMLLMILTTGREKSEKDWKKLSLDDVGFSDYKIIPILGLRSLIKVYPV
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University