Enzyme

Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.

COMT

Enzyme

Flavone 3'-O-methyltransferase

Enzyme name

EC:2.1.1.76

Entry

Transferases;Transferring one-carbon groups;Methyltransferases

Class

S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase

Sysname

S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]

Reaction

S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]

Product

Species information

Camptotheca acuminata

Species name

Camptotheca acuminata is a species of eudicot in the family Nyssaceae.

Species info

Gene sequence info

Camptotheca_acuminata.COMT.fa

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>Cac_g005435.t1
MEYGSEIYGDEEEQCSYAMQLVTSSALPMVMQAAVELGLLEIIAKAGPGRQLSPTEIASQMPTTNTVAAAMLDRILRLLASHSVLTCSVVDADDGHLQRLYGLAPVAKYFVKNEDGVSLGPLMALIQDKVFMDSWYVLKDAVVEGGIPFNRVHGTHAFEYPATDPRFNEVFNKAMLNHTTIVIKRILQSYKGFDQLKQLVDVGGGLGVTLNMITSKYSTIKGINFDLPHVIQHAPPYPGVEHVGGDMFASVPKGDAIFMKWILHDWNDDQCLKLLKNCYKSLPDNGKVIVVEAILPVIPDARAAVKSTCQIDLVMMTQNPGGKERTEEEFLALATGAGFSGIRLESFVWNFWVMEFYK*
>Cac_g023631.t1
MESKEGVDYTTVWGLGGLIGTQMATKAAIELNVFNIISDSGPEAKLSAAEIVSKIPTSNPNAATVLDRILRVLSAKSILSSSLRPCPNGQERAYGLSMVSRALVSNTDTVCLSPALLMATQMEFVESLYLLKNMVLEPEYCVPFEKAHGVNFFDYAAKDPKVNKMFNESMACHSKMAFEMVFSNKVYTGFGQVKELMDVGGGNGTLLGRIVSVYPHIHGINFDLPHVIADAPKFPGVEHVAGDMFEPLPNVETILLKSVLHDWDDHNCIKLLKNCWKALPANGKWKVVEMQTTNGGGLGEE*
>Cac_g015644.t1
MEGKEGFDYLPLWHLGSLIGTQMAIRAAIELNVFDIINDAGPEAQLSSAEIVSKIPTTNPNAATALERILRMLSAKSLLSTSLRPCQNGEMMANQEERTYGLTMASRALVTDKDGVSLAQVILSSSQREAVESLYMLKNVVLEAECLPFSKAYGVGYFEYLAKDPTSSRLFNQSMEWLSKLMLDQVFKLYRGFEEVTELMDVGGGNGTSIGKIVSKYPHIQGLNFDLPHVIADAHNFPGVKHVAGDMFESLPNAQTILLKSVLHDWDDDGCMKLLRNCWNVLGADGKVVVIEFAIPQEWDNGAEIAIAMDLCMMVAFGGKERSTAEFTELAKAAGFTQINIIPIAQGLHVFEFIKTNC*
>Cac_g023630.t1
MESKEGVDYTTVWGLGGLIGTQMATKAAIELNVFNIISDSGPEAKLSAAEIVSKIPTSNPNAATVLDRILRVLSAKSILSSSLRPCPNGQERAYGLSMVSRALVSNTDTVCLSPALLMATQMEFVESLYLLKNMVLEPEYCVPFEKAHGVNFFDYAAKDPKVNKMFNESMACHSKMAFEMVFSNKVYTGFGQVKELMDVGGGNGTLLGRIVSVYPHIHGINFDLPHVIADAPKFPGVEHVAGDMFEPLPNVETILLKVRFLSVNSLILLLIFCHFTVIHFSHPDLLNFSSIPRSTSIFTKLHNHPLRRPNTPLLRRPRSHELKDHVAPQRPHKPEAMAKPNNGGEGWQRRGDSLNSPNRSSTLFSLTARISNKAHLEW*
>Cac_g027446.t1
MSSTENHDSLSIEEDDEACAYALQLSLSHIPHMVMNAAVELQLFDIIAQAGSGEHISSSEIVSQLPAQNPDAASVLDRMLCLLASYSLLTCSLRTHKDGTVERLFGLAPAGKFFVRNEGGRSLATMFPFTCYPPTWNPWFHLKDAILEEGNIFKKACGMHIFQFMNGDPTYNSLFNRSMADISSVTMMKVLETYKGFDGLSTLVDVGGGTGTSLNMIISKYSFIKGINFDLPHVIKRAPSYSGVEHVGGDMFVSIPEGEAIMAKNVFHNWNDEHCLKILNNCYEALPKKGKVIVIDVVMPLAPEPSPASKFAVLADICMLHTPSGKERTEKEFEALSKAAGFSDFKIICSAHAFGVMEFYK*
>Cac_g010964.t1
MGSTSEVQTCPAEAEEEACLFAMQLASASVLPMVLKSAIELDLLETMAKSGPGAYISPSDLAAQLPTQNPDAPIMLDRILRLLTSYSVLNCTLRNLPDGRVERLYGLAPVCKFLTRNADGVSMAPLLLMNQDKILMESWYYLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILETYKGFEGLKSVVDVGGGTGATLNMIVSKHPTIKGINFDLPHVIEDAPSYPGVEHAGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVSPDSRLATKNVIHIDVIMLAHNPGGKERTEKEFEALAKGSGFQGFRVMCCAYNTWVMEFLKKI*
>Cac_g032399.t1
MSCAEKERTASSIPISIQEEDDESCVYALQLSLSHVLPMVLNAAIELHLFDIIAGAGPDAQLSPSQIASKLPTQNPHAADMLDRMLRLLGSHSLLSCSLHTLEDGTIERRYGLSPAGKFFAENRDEPSLASVVSLANHPAPAEVRCNLKDAILEGGNQFKKVHGMSIFQYMNIDNTLNKVFNKAMADISSSVMKKILEVYKGFEGLTSLVDVGGGIGKSLNMIISMYPSIKGINFDLPHVIQSAPSYPGIEHVGGDMLVSIPKGDAIMIKNACHNWSDDHCVKILNNCYEALPENGKAIIVDIVMPMAPESSNAAKYGTQLDNLMLLEPGGKERTEKDFEALFKASKFSNYQIVCCAYAFSVIEFYK*
>Cac_g033440.t1
MSSTENHDSLSIEEDDEACAYALQLSLSHIPHMVMNAAVELQLFDIIAQAGSGEHISSSQIVSQLPAQNPDAASVLDRMLCLLASYSLLTCSLRTHKDGTVERLFGLAPAGKFFVRNEGGRSLATMFPFTCYPPTWNPWFHLKDAILEEGNIFKKACGMHVFQFMNGDPTYNNLFNRSMADISSVTMMKVLETYKGFDGLSSLVDVGGGTGTSLNMIISKYSFIKGINFDLPHVIKSAPSYSGIEHVGGDMFVSIPEGEAIMVKNVFHNWNDEHCLKILNNCYEALPKKGKVIVIDVVMPLAPEPSPASKFAVLADICMLHTPSGKERTEKEFEALSKAAGFSDFKIICSALAFGVMEFSK*
>Cac_g029338.t1
MSSTSENQTSSAEAEEEACLFAMQLASASVLPMVLKSAVELDLLETMAKSGPGAYISPSQLASQLPTKNPDAPVMLDRILRLLTSYSVLDCRLQNLPDGRVERLYGLAPVCKFLTRNADGVSMAALLLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDTRFNKVFNEGMSNHSTIIMKKILEIYKGFEGLASVVDVGGGTGATLNMILSKYPAIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSVPKADAIFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPIAPDSRLATKNVIHIDVIMLAHNPGGKERTEKEFEALAKGAGFQGFQLMCCAYNTWVMEFLKKV*
>Cac_g015645.t1
MGSQEGFDYLSLWHLGSLIGTQMALRAAIELNVFNIINDAGPDAQLSSAEIVAKIPTTNPNAATALERILRMLSAKSFFSTCIRPCQNGKIMANHEERTYGLTMVSHALVTDKDGASLAQGILFSSEREVVESLYMIKNAVLEPDCLPFNKANGVSFFEHIGEDPKSNRLFNQNMEWLSKILFDKVFKVYTGFDEVKELMDVGGGNGTSLGKIVSIYPHIHGINFDLPHVVADAYKFPG*
>Cac_g023629.t1
MESKEGVDYTTVWGFGGLIGTQMAIKAAIELNVFNIISDSGPEPKLSAAEIVSKIPTSNPNAATVLDRILRVLSAKSILSSSLRPCPNGQERAYGLSMVSRALVSNTDTVCLTPALLMATQKELVDSLYLLKNMVLEPEYCVPFEKAHGDNFFDYAAKDPKVNKMFNESMACHSKMAFEMVFSNKVYTGFGQVKELMDVGGGNGTLLGRIVSVYPHIRGINFDLPHVIADAPKFPGVEHVAGDMFEPLPNVETILLKSVLHDWDDHNCIKLLKNCWKALPANGKVIVIESAVPEVSDNDAELQTEIIMDMAMMVVTGGKERSTAEFDNLAKAGGFAETRIFHIAQGIHVMEFIKKISE*
>Cac_g000171.t2
MSSGENKIITSSKEEEEEEINCQYAMQLACSSVLPMVLKASVELGLFDIIERAGPGALLSPTEIASRLPTNNPDAPHLLDRMLRLLASHSILNCSVDAQHDGHQVHRLYGLAPISKYFIRNQDRVSLAPFLDMLQSKVMIDIWYHMKDAVVEGGLPFNRAYGMNSSEYVGKDAKFLELFKASMREYNSMFMRMILDKYKGFEDLKSLVDVGGGDASILNMIVSKYPTIKGINFDLAWIIDKSPTYLGIEHVAGDMFVSIPKGDAIFMKWMLHSWDDEHCLKILKNCYEALPEHGKVIIVDLVVPEAPETSIAGKSVLQLDLFLMNMNPGGKERTEKEFEILAKAARFSSIRVASCAYGLSVVEFYKNM*
>Cac_g029514.t1
MASDARLVANVLLKECKGVFEGLNSLIDVAGGTGTMAKTIAEAFPHLKCTVFDLPHVVSNLQASKNFNFLVGDMFEAIPHADAIFFKDKQGTFSKELEMLKHDMNTLKDTADQPKENFRGEFRDQLGKYFSQFQKVQGKHPIEDTSRHKPISQYHEEFPISHLTIELPQVLNSHRRVLPTPEFSLTPHIPNHVLRADRTHETTTKAYFPNVFRHLIGQGYLLDHLTEFEEDEFLSIGAREYQRPFRPHE*
>Cac_g004005.t1
MSTTTNSKPMFHFHHEQQQEDHDQQEVGTLAIRLANAVVLPMVLKSALELNLIDIISASTDSGEFLSPSEIAAQLPTRNPDAPILLDRMMRLLASYGVLKCSLKTGENGEVKRLYGTGPIWGIPFNRAYGMTAFEYAGIDQRFNKVFNQAMSNNATLIMNKMLDIYKGFEGVKVLVDVGGGIGATLNTIISKYPHIMGINYDLPHVIANAPSYPGMKHVEGDMFVSVPKGDAIFMERILHDWSDEHCLKILKKCYEALPDNGKVIIVEYILPEAPDNNVSSNIAFELDLFMLAQTPDSYPASRFSLSSFLPNFDTKIKGRNILIIMTSDYALKISAELESASRLRTAQFIFTRRPWLDLYGINVRPVAPFGSTSSKPFVDTALIHRCLPDELLFEIFARMTPYNLGKAACVCRKWRYTIRNPVFWRNACLKAWQLSGVVENYKILQSKHDGSWRKMWLLRPRIRTDGLYISRNTYIRAGVAEWKITNPVHVVCYFRYLRFYPSGRFLYKNSSQKVKDVAKCMNIRASKVDCVYSGHYTLSDDKVEAAFLYPGWRPTVWRIRLRLRGTTAGANNRMDLLSLVTSGVDDSEVNSPDEDILGVVEGWQEDETHNPDVPAVSHRRGLTPFVFVPFEEVEISVLNLPVERMDYFVPG*
>Cac_g023632.t1
MESKEGVDYTTVWGFGGLIGTQMAIKAAIELNVFNIISDFGPEPKLSAAEIVSKIPTSNPNAAAVLDRILRVLSAKSILSSSLRPCPNGQERAYGLSMVSRALVSNTDTVCLAPALLMATQKELVDSLYLLKNMVLEPEYCVPFEKAHGVNFFDYAAKDPKVNKMFNESMACHSKMAFEMVFSNKEYTGFGQVKELMDVGGGNGTLLGRIVSVYPHIRGINFDLPHVIADAPKFPG*
>Cac_g023628.t1
MAIKEAAIELNIFNIISDSGPDAKLSAAEIVSKIPTSNPNAATVFDRILRVLSAKSILSSSLRPCSNGQERAYGLSMESRALVSNTDTVCLAPALLMATQKEFVESFYLLKNMVLEPEYCVPFEKAHGVNFFDYAAKDPKVNKMFNESMACHSKMAFEMVFSDKVYTGFGQFKELMDVGGGNGTLLGRIVSVYPHIRGINFDLPHVIADASKFAG*

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University