Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Camptotheca acuminata
Species name
Camptotheca acuminata is a species of eudicot in the family Nyssaceae.
Species info
Gene sequence info
Camptotheca_acuminata.FLS.fa
Download
>Cac_g003948.t1
MEVERVQAIASLSERMDTIPSEFIRSENEQPAITTVHGMVLEVPVIDLGHPDEENLVRLIADASREWGIFQVVNHGIGSDVISKLQKVGKDFFELPQEEKEVYAKPAGAQTVEGYGTKLQKDVEGKKGWVDHLFHKIWPPSAINYQFWPKSPPTYREANEEYAEKLQGVGDKLFKSLSLGLGVEGHELKDAVGGEDLIYLMKINYYPPCPRPDLALGVVPHTDMSSITILVPNEVQGLQVFKDGHWYDVKYIPNALIIHIGDQIEILSNGEYKSVFHRSTVNKEMARMSWPVFLEPPPEIEIGPLLKLINEENPAKYKTKKYKDYAYCKLNKLPQ*
>Cac_g003949.t1
MYKGGIEPAVDKLKLASDWWWTSEVVLAVDGNATVIILSNGKYKSVLHRTTVNKEKTRMSWPVFLEPPPTFVVGPHPKLVNESNPPKYKTKQFSDYVYCKLNKIPQ*
>Cac_g003954.t1
MPPIQFAALKLFKSVVTEEYNVEILRVTKELLELLLKGLGLDMKALKSCLGGEDIELEMKINMYLLCPQPQLTLGVEPHTDMSTLTILVLNDVPGLQIWKYGNWVAVNYLPNALLVHVGDNIEVLSNGKYKSVLHRSLVNKEKTRMS*
>Cac_g008674.t1
MAETTPHAVLEPLSKPVQGQVINGDELPQRYVYKAGDGVISASFPPMEAINHGTASSFLDEVHGVAKQFFALPMEEKQKYSRTVDDIEGYGNDTVLSEN*
>Cac_g003952.t1
MEVERVQAISSDSVSNDTIPTEFIRPENEQPGITTMGQASEVPIVDFGIEDQEKLVQSIVDASCQWGMFQIVNHEIPSDVITKLQLVGKEFFDLPQEEKEVYAKIPGSESVEGYGTKLQKEVEGKKGWVDHLFHKIWPPSAINLKFWPKNPPSYREATEEYAKYLHVVVDKLLGSLSKGLGLEENELKKAVGGDELVYMMKINYYPPCPRPDLALGVVAHTDMSAITILVPNDVQGLQLFKDDYWYDVKYIPNALVIHIGDQTEVLL*
>Cac_g008892.t1
MEAVRVQALASLSKCMDTIPSEFIRSENEQPAITTVHGTILEVPVIDLGKPDEENLVRSITEASRDWGIFQVVNHGIGGDVIRKLQKVGKEFFELPQEEKEACAKPPGAQSVEGYGTKLQKEVEGKKGWVDHLFHKIWPPAAINYQFWPKNPPSYREANEEYALQLRGVAEKLFKCLSLGLGVEGHELKEAAGGEDLIYLMKINYYPPCPRPDLALGVVAHTDMSTITILVPNEVPGLQVFRDDHWYDVKYIPNALIIHIGDQIEILSNGKYKSVFHRSTVNKETARMSWPVFLEPPPELEIGPLPKLINEENPAKFKTKKYKDYVYCKLNKLPQ*
>Cac_g006858.t1
MAISPESLRENPIDFRAPPPSPIAAGRRSFVTNDDVLSDFLEHSLRVPDLILPDRVFPRQKSIQTPPTIDFQSLNSLEKDSIAKIIESTARIGCFQLVNHGIPYDLIVSVSSAGAGIFQISPEKKAMVSRSPEKPYGFEEFRGEEESEMSEEFVWYRDEGLKLEMEGIWPLGYSSFSEKMERLLCDIEKVAGKIVVALQENVPRKSRFGNSMIEGQEVGGTICYLYKHFRNAVEDQRWVSSLKYDVIRMLVRGSDYSHALCFHVCDGSSEFHVYSKKGWVSFCPDKHALVITIGDQIQAWSGGQYKHVIGRPIFKGEDEGCITMAFLCSPPNITTKCETEKGKIISLGQQAIMAIVLTFVYHFLTFIYNKF*
MEVERVQAIASLSERMDTIPSEFIRSENEQPAITTVHGMVLEVPVIDLGHPDEENLVRLIADASREWGIFQVVNHGIGSDVISKLQKVGKDFFELPQEEKEVYAKPAGAQTVEGYGTKLQKDVEGKKGWVDHLFHKIWPPSAINYQFWPKSPPTYREANEEYAEKLQGVGDKLFKSLSLGLGVEGHELKDAVGGEDLIYLMKINYYPPCPRPDLALGVVPHTDMSSITILVPNEVQGLQVFKDGHWYDVKYIPNALIIHIGDQIEILSNGEYKSVFHRSTVNKEMARMSWPVFLEPPPEIEIGPLLKLINEENPAKYKTKKYKDYAYCKLNKLPQ*
>Cac_g003949.t1
MYKGGIEPAVDKLKLASDWWWTSEVVLAVDGNATVIILSNGKYKSVLHRTTVNKEKTRMSWPVFLEPPPTFVVGPHPKLVNESNPPKYKTKQFSDYVYCKLNKIPQ*
>Cac_g003954.t1
MPPIQFAALKLFKSVVTEEYNVEILRVTKELLELLLKGLGLDMKALKSCLGGEDIELEMKINMYLLCPQPQLTLGVEPHTDMSTLTILVLNDVPGLQIWKYGNWVAVNYLPNALLVHVGDNIEVLSNGKYKSVLHRSLVNKEKTRMS*
>Cac_g008674.t1
MAETTPHAVLEPLSKPVQGQVINGDELPQRYVYKAGDGVISASFPPMEAINHGTASSFLDEVHGVAKQFFALPMEEKQKYSRTVDDIEGYGNDTVLSEN*
>Cac_g003952.t1
MEVERVQAISSDSVSNDTIPTEFIRPENEQPGITTMGQASEVPIVDFGIEDQEKLVQSIVDASCQWGMFQIVNHEIPSDVITKLQLVGKEFFDLPQEEKEVYAKIPGSESVEGYGTKLQKEVEGKKGWVDHLFHKIWPPSAINLKFWPKNPPSYREATEEYAKYLHVVVDKLLGSLSKGLGLEENELKKAVGGDELVYMMKINYYPPCPRPDLALGVVAHTDMSAITILVPNDVQGLQLFKDDYWYDVKYIPNALVIHIGDQTEVLL*
>Cac_g008892.t1
MEAVRVQALASLSKCMDTIPSEFIRSENEQPAITTVHGTILEVPVIDLGKPDEENLVRSITEASRDWGIFQVVNHGIGGDVIRKLQKVGKEFFELPQEEKEACAKPPGAQSVEGYGTKLQKEVEGKKGWVDHLFHKIWPPAAINYQFWPKNPPSYREANEEYALQLRGVAEKLFKCLSLGLGVEGHELKEAAGGEDLIYLMKINYYPPCPRPDLALGVVAHTDMSTITILVPNEVPGLQVFRDDHWYDVKYIPNALIIHIGDQIEILSNGKYKSVFHRSTVNKETARMSWPVFLEPPPELEIGPLPKLINEENPAKFKTKKYKDYVYCKLNKLPQ*
>Cac_g006858.t1
MAISPESLRENPIDFRAPPPSPIAAGRRSFVTNDDVLSDFLEHSLRVPDLILPDRVFPRQKSIQTPPTIDFQSLNSLEKDSIAKIIESTARIGCFQLVNHGIPYDLIVSVSSAGAGIFQISPEKKAMVSRSPEKPYGFEEFRGEEESEMSEEFVWYRDEGLKLEMEGIWPLGYSSFSEKMERLLCDIEKVAGKIVVALQENVPRKSRFGNSMIEGQEVGGTICYLYKHFRNAVEDQRWVSSLKYDVIRMLVRGSDYSHALCFHVCDGSSEFHVYSKKGWVSFCPDKHALVITIGDQIQAWSGGQYKHVIGRPIFKGEDEGCITMAFLCSPPNITTKCETEKGKIISLGQQAIMAIVLTFVYHFLTFIYNKF*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University