Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Calotropis gigantea
Species name
Mudar (Calotropis gigantea) is a species of eudicot in the family Apocynaceae (dogbane family).
Species info
Gene sequence info
Calotropis_gigantea.COMT.fa
Download
>cal_g017376.t1
MDSSVMTFNNQEDIDHCSYALQLVFSGALPMVLNTVIKLNVFEIIAKAGHGAQLSPSQIVSQMPTENPEAPIMLDRMLRMLASFSLFTSSLVDDDPASHGSAHRVYGLGPVSKYFIKNENGASLGPLLDLLQDKVLTDIWYELGSAVLEGGTAFNRAHNMHIFKYTGLDQKFNETFNTAMINHTKVVVQKVLECYRGFEHLKTLVDAGGGLGITLGMITSKYPNLKGINYDLPHVIERAPTYPGVEHIGGDMFDSVPRADAIFMKWILHDWDDGHCIKLLRNCYKALPKNGKVIALDAILPMNPDNSSSTKHTTQVDLFTLALYHPGGKERTEQEFQALASKAGFAGIAKVCVSCDLWVMELYK*
>cal_g015650.t1
MDSSPTILMNQTYVNESGEEEEQLFSYAMQLVTAASLPMVLLSAIRLDVFEIIAKAGQDAQLSPSQIAASMPCKNPDAPSMLDRMLRLLASYSILTWSVAGEGPLPRVYGLAPVAKYFVRNSESEGASLGGLLGLLQDKVFIDSWYELEDAVREGGIPFNRVHGTHAFEYPGRDPRFREVFNKAMADHTTIAINRILERYKGFEHLKMKSLVDVGGGLGITINIITNKYPSIRGFNFDLPYVIQHAPAYPGVEHVGGDMFQNVPKGDAIFMKWILHDWDDDHCLKLLQNCYKALPEGGKVIVVDALVPVKPDTSARGKSTCQSDLIMLTQNPGGKERSEQEFLALATAAGFQGISIECFVCNLWVMEFYK*
>cal_g017374.t1
MDDSSEMNFQNEEDTNHCSYALQMVFSGALPMVLNTVIKLNVLEIIAKAGHGAQLSPSQIVSRMPTENPEAPIMLDRMLRMLASFSLFTSSLVDDDPTSHGSAHRIYGLGPVSKYFIKNENGASLGPLLDLLQDKILTDIWYELGPAVLEGGTAFNRVHKMHIFKYTGLDQMFNEKFNTAMINHTKVAMQKVLECYIGFEHLKTLVDAGGGLGITLGMITSKYPNLKGINYDLPHVIEHAPKYPGVEHISGDMFDSVPRADAIFMKWILHDWDDGHCIKLLKNCYKALPKNGKVIALDAILPMNPDNSSSTKHTTLVDLFTLALFNPGGIERTEQEFQALASKAGFAGIAKVCVSCDLWVMEFYK*
>cal_g017379.t1
MDDSSEMNFKNEEYTNHCSYALQMVFSGALPMVLNTVIKLNVFEIIAKAGHGAQLSPSQIVSQMPTENPEAPIMLDRMLRMLASFSLFTSSLVDDDPTSHGSAHRIYGLGPVSKYFIKNENGASLGPLLDLLQDKILTDIWYELGPAVLEGGTAFNRVHKMHIFKYTGLDQMFNEKFNTAMINHTKVAMQKVLECYTGFEHLKTLVDAGGGLGITLGMITSKYPNLKGINYDLPHVIEHAPKYPGVEHISGDMFDSVPRADAIFMKWILHDWDDGHCIKLLKNCYKALPKNGKVIALDAILPMNPDNSSSTKHTTLVDLFTLALFNPGGRERTEQEFQALASKAGFAGIAKVCVSCDLWVMEFYK*
>cal_g008557.t1
MAGEDEAAACLYAMQLASASVLPMVLKSAIELDLLELIKKAGPGAYVSPSELASQLPTQNPDAHIMLDRILRLLASYSVLECTLKNLPDGGGVERLYGLAPVCKYLTKNEDGVSMAPLLLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMTAFEYHGTDSRFNKVFNQGMSNHSTIIMKKILETYQGFEGLKTVVDVGGGTGATLSMILSKYPNIKGINFDLPHVIEDAPKSHPGVQHVGGDMFVSVPNGDAIFMKWICHDWSDEHCLKFLKNCYKSLPENGKVILAECVLPEAPDTTLETQNVVHVDVIMLAHNPGGKERTEKEFEALAKKAGFKDFRKICCAYNYTWIMELLK*
MDSSVMTFNNQEDIDHCSYALQLVFSGALPMVLNTVIKLNVFEIIAKAGHGAQLSPSQIVSQMPTENPEAPIMLDRMLRMLASFSLFTSSLVDDDPASHGSAHRVYGLGPVSKYFIKNENGASLGPLLDLLQDKVLTDIWYELGSAVLEGGTAFNRAHNMHIFKYTGLDQKFNETFNTAMINHTKVVVQKVLECYRGFEHLKTLVDAGGGLGITLGMITSKYPNLKGINYDLPHVIERAPTYPGVEHIGGDMFDSVPRADAIFMKWILHDWDDGHCIKLLRNCYKALPKNGKVIALDAILPMNPDNSSSTKHTTQVDLFTLALYHPGGKERTEQEFQALASKAGFAGIAKVCVSCDLWVMELYK*
>cal_g015650.t1
MDSSPTILMNQTYVNESGEEEEQLFSYAMQLVTAASLPMVLLSAIRLDVFEIIAKAGQDAQLSPSQIAASMPCKNPDAPSMLDRMLRLLASYSILTWSVAGEGPLPRVYGLAPVAKYFVRNSESEGASLGGLLGLLQDKVFIDSWYELEDAVREGGIPFNRVHGTHAFEYPGRDPRFREVFNKAMADHTTIAINRILERYKGFEHLKMKSLVDVGGGLGITINIITNKYPSIRGFNFDLPYVIQHAPAYPGVEHVGGDMFQNVPKGDAIFMKWILHDWDDDHCLKLLQNCYKALPEGGKVIVVDALVPVKPDTSARGKSTCQSDLIMLTQNPGGKERSEQEFLALATAAGFQGISIECFVCNLWVMEFYK*
>cal_g017374.t1
MDDSSEMNFQNEEDTNHCSYALQMVFSGALPMVLNTVIKLNVLEIIAKAGHGAQLSPSQIVSRMPTENPEAPIMLDRMLRMLASFSLFTSSLVDDDPTSHGSAHRIYGLGPVSKYFIKNENGASLGPLLDLLQDKILTDIWYELGPAVLEGGTAFNRVHKMHIFKYTGLDQMFNEKFNTAMINHTKVAMQKVLECYIGFEHLKTLVDAGGGLGITLGMITSKYPNLKGINYDLPHVIEHAPKYPGVEHISGDMFDSVPRADAIFMKWILHDWDDGHCIKLLKNCYKALPKNGKVIALDAILPMNPDNSSSTKHTTLVDLFTLALFNPGGIERTEQEFQALASKAGFAGIAKVCVSCDLWVMEFYK*
>cal_g017379.t1
MDDSSEMNFKNEEYTNHCSYALQMVFSGALPMVLNTVIKLNVFEIIAKAGHGAQLSPSQIVSQMPTENPEAPIMLDRMLRMLASFSLFTSSLVDDDPTSHGSAHRIYGLGPVSKYFIKNENGASLGPLLDLLQDKILTDIWYELGPAVLEGGTAFNRVHKMHIFKYTGLDQMFNEKFNTAMINHTKVAMQKVLECYTGFEHLKTLVDAGGGLGITLGMITSKYPNLKGINYDLPHVIEHAPKYPGVEHISGDMFDSVPRADAIFMKWILHDWDDGHCIKLLKNCYKALPKNGKVIALDAILPMNPDNSSSTKHTTLVDLFTLALFNPGGRERTEQEFQALASKAGFAGIAKVCVSCDLWVMEFYK*
>cal_g008557.t1
MAGEDEAAACLYAMQLASASVLPMVLKSAIELDLLELIKKAGPGAYVSPSELASQLPTQNPDAHIMLDRILRLLASYSVLECTLKNLPDGGGVERLYGLAPVCKYLTKNEDGVSMAPLLLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMTAFEYHGTDSRFNKVFNQGMSNHSTIIMKKILETYQGFEGLKTVVDVGGGTGATLSMILSKYPNIKGINFDLPHVIEDAPKSHPGVQHVGGDMFVSVPNGDAIFMKWICHDWSDEHCLKFLKNCYKSLPENGKVILAECVLPEAPDTTLETQNVVHVDVIMLAHNPGGKERTEKEFEALAKKAGFKDFRKICCAYNYTWIMELLK*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University