Enzyme
Catalyses the final step in the formation of the isoflavonoid skeleton. The reaction also occurs spontaneously.
HIDH
Enzyme
2-hydroxyisoflavanone dehydratase
Enzyme name
EC:4.2.1.105
Entry
Lyases;Carbon-oxygen lyases;Hydro-lyases
Class
2,4',7-trihydroxyisoflavanone hydro-lyase (daidzein-forming)
Sysname
(1) 2,4',7-trihydroxyisoflavanone = daidzein + H2O [RN:R07317];(2) 2,4',5,7-tetrahydroxyisoflavanone = genistein + H2O
Reaction
daidzein [CPD:C10208];H2O [CPD:C00001];genistein [CPD:C06563]
Product
Species information
Calotropis gigantea
Species name
Mudar (Calotropis gigantea) is a species of eudicot in the family Apocynaceae (dogbane family).
Species info
Gene sequence info
Calotropis_gigantea.HIDH.fa
Download
>cal_g016016.t1
MDSTPSSSSDNEVLYDFPNAFRIYKNGRVERYLKTPFVPPSPSVEDDDDDDNDASICRSKDVEISPENINKVSARIFLPKSVSKGEKLPVLIYIHGGAFVIGSAFSSTYHSYVSSVVARANVLAVSIQYRLAPEHPIPTCFEDCWETVKWITSHSIDGQGQDAWINDHADFSRVFLAGDSAGGTITQNMMVRASSSASCVEDGAVKIVGMVLIHPYFGSGENEKLWEILCSDETGPDDPRLNPMAHLNLLQDLKCSKVLIFIAEKDWIRDRGWIYYETLKKSGWNGHVEIVESLGEDHVFHLMNPNCENANFLMEKIVSFLKD*
>cal_g015614.t1
MDSAPTREAMLSINLTPSNEEVAFEFLPFFRAFKDGRVERFFGTDVVPPTSTEPFPDGVCSKDVQIVAEKKVSGRLFLPSTTKIEPNHQKLPLLIYIHGGAFCFGSPFSQAYHSYLTSVVKEANVVALSIDYRLAPENLLPAAYEDAWDTLKWVVSHADGFGAEPWLNDHADLQRVFVTGDSAGGNISANTVAQAGVQNLAGMKIQGLCLIHPDFASKEGEVYKSWNFACPSSTGGDDPRMNPAKDSRLPNLGCEKVLVCVAGDDPMKERGYYYYDTLKNSGWKGSVEFVETPGESHVFHLFNPTGENALILLRKIAAFMN*
>cal_g015616.t1
MDSIPSDEITVDFFPFFRTYKDGRIHRYNKPTIVPATDDPITGIRSKDVVVSPDNKVSARLFLPGGVEKDEKLPVLVYMHGGAFVIESAFSSQYHSYGSSVAAEAKVLALSVEYRLAPEHPIPACFEDSWAALKWVESHAKDRQGPEPWINNHADFTRIYVGGDSAGGNIAHNTVSRASSEGLEDGMKIVGMILAHPYFGSGQTEKLWDAICSDSDNKGPEDPRLNPMAHPNLLSNLKCHKVLICTGEKDFIRDRGWLYYEALKKSGWSGQVEIFDYEGEGHVFHLLNPKCDNAGIYMKQVVAFLKD*
>cal_g007634.t1
MAISVDYRRVPENPLSAAYEDSWEVLNWVSLHNNSSGEEGSEDWIWDGVDFGKVFLSGDSAGANISHHMAIRAGTESSILEFRGIILIHPYFWGEKPVGLIEIKDPIRKAMVDKWWKFVCPSDKGCDDPLINPFVNNNDSYEICADHSNLGCKRILICVAENDILNERGKMYYRSLIRSKIWKGKAEIVETEGEDHVFHIFNPSCEKAESLVKKCAAFLNQV*
>cal_g008599.t1
MASSSENIATDLSPMLKVYKDGTVKRLYNTTYVPPTSDPDPKTGVSSKDVNITSSISARLFLPSIINSTDQRLPILVYFHGGGFCLESAFSEINHNYINLIVSEAKVLAVSVEYRLAPEHPLPTAYEDCWEAVQWVVSRNNKEPWIINHGNTDKLYIGGDSSGGNIVHNIAMKAGQNHHQEVKILGGFLCCPYFWGSKCKSETDSTINEDGVSLAYRLWMFAYPNADNGIDNPMINPLADDGLSEIGCLRLLMIEGENDVFKETSFTYVQGLKKSGWKGEVELVVVEGEGHCFHLFNLDSEKARCLIKRLASFINA*
>cal_g008598.t1
MGKRREKFEHFNYKQTPSPTVIKPDSCLVHFRLSVLSSHLRSVTDSSEESKFLLGVSSSNCASVAKVIWENECVFCKIVTGQAPALKLTETGLMYVEALKKSRWKGDVDLVDVAGEETDPETAKNLIRRIASFIRK*
>cal_g008602.t1
MVCSNKEVSSEFLPFLQVYKDGSVERFLDSPYVPPSTGSTGDNVSSKDITITGDISARIYLPNSHHHHQLLPVLVYFHGGGFCIESAFSFLSHRYITTLVSHAKILVVSVEYRRAPENPLPAAYQDSWTALQWIASQSVEKTEPWLSNHANFNRVFVGGDSAGANIAHNLAMKASLEDMQNKIHLYGVLLSQPYFLAGSSEKYSNVNDEDEKRLLLYRMWEFVYPNAPGGFDNPLINPFSGDAPSLSWLGGSRLLVCVAEKDELRERGVRYFDAVKKSGWRGKMEFFEVEGEDHCFHIFNLDSQNAQIMIKRLASFLNS*
>cal_g008597.t1
MSSSSSSEDIVSDLSPYIRVYKDGTVERLFSSPFVPPSPNPENGGVSSKDIKISSEVSARVYLPLTDDDEKLPILVYYHGGGFCLESAFSFLDHRYLNLLVSQGKCIAVSVEYRLAPENPLPAAFQDSWTALQWVTSHHHHPAAGVEGDPWLLKHGDFNKLYLGGDSAGGTIVHYLAMRAGVESLPGDVKITGGFLSHPYFWGSKSEQQLINYKKGGAGEVRAGEYNSYPYRMWMLAYPSVPGGIDNPLINPIAEDAPSLLGMTCSRLLVIVAEKDELRETGLMYVEALKKSGGKGDVDLVDVEGEEHCFHIFNTDTEKAENLIRRIASFIRTN*
>cal_g008596.t1
MASSTSSSSEIVTDLSPFIRVYKDGKVERLLGSPFVPACTEHPATGVSSKDVNISPQVSARLYLPLTESIESSEKLPILIYYHGGGFCLDSAFDFLCHRYMNLLVSQAKAVAISVNYRLAPEYPLPAAYEDSWTALTWVASHSVSDIDIGGDKEPWLLNHGNFDKLYLVGDSCGGNIVHNLAIKAGAEILPGDVKIAGGIISHPFFWGSKFESSRTAETTSSDQPPHNHTVGTYYYRLWLFAYPSAPGGIDSPMVNPFPLFSTGEEADNTPPPPPSLSGIGCRKLLVCTSEKDDIRETSVLYFEALKKSGWKGEVEFVDIEGEGHCFQIFKPDTENAKNFISRMASFINS*
>cal_g009982.t1
MRKRRMAAMTLDPSLGLQVNKEHQKHKVNVVEEIDGLIRVYKDGYVERPQIIPTVHSMVAPEPGVTSRDVIIDKGNNIWARFYIPLFQLNMMMMPLIVYFRGGGFCVGSASWICYHEFLAKLAAKSKCVIMSVNYRLAPENPLPAAYDDGLKALFWVKENALTGANEWFSSKCNFASIFLAGDSAGGNIAYSIMSSSRFKLSSSFKPLNFKGAILIQPFFGGETRTHSEKYMVQPPGSALTIAASDTYWRLSLPKGANRDHPWCNPIGKSSPKLENLELLPTLICISEMDILKDRNLELFSGLVSAGHKVECMMYKGVGHAFQILNKSQISQERTEELICHIKNFIFR*
>cal_g015221.t1
MADIRIPGVVKAIDAAANPPYEVDECRGVLRVFSDGSILRSSNPSFIVPVSDDGSVLWKDVVFDATQNLRLRLYKPAAASPISAKLPIFYYIHGGGFCIGSRTWPNCQNYCFKLALDLQAVIISPDYRLAPENRLPAAIEDGFAAVKWLQSQAASEDPDRWLTDVADFGRVFISGDSAGGNIAHNLAVMLGAGSPGLAPVKVKGYVLLAPFFGGTVLSKAEAEGPKDAFLNWELMDRFWRLSVPDGETTDHPLINPFGPVSPSMENLDLDPILVVVGGSDLLKDRADDYAKRLKIWGKKVEYVEFEGQQHGFFTINPNSEPSLELMEVIKKFISKYSA*
>cal_g015615.t1
MATNCSDILHDFFPLLRVYKDGRVERLREQQFVPASVDQETGVECKDVKISKEFDVSARLYLPRNSKPGDKIPLLVYFHGGGFVIESAFSPEYQNHLNLLVREANVLMVSVDYRLAPEHPLPSAYEDSWLVLQWIASHSGGKSSDSEYEAWIKDYADFNRVYFGGDSAGGNIAHNMAMKVGLEGLDGVNLVGIFLNCPYFWGKDPIGDETSNAEIKKYLDNLWLFVYPKTTGLDDPLLNPAADPSLSKLGCKRVLIFVAGQDPLRHRGRHYKEALEKSGWGGIAEVMEFEGEFHVFNLSSPICEKALSMLKTLASFLNQSGL*
MDSTPSSSSDNEVLYDFPNAFRIYKNGRVERYLKTPFVPPSPSVEDDDDDDNDASICRSKDVEISPENINKVSARIFLPKSVSKGEKLPVLIYIHGGAFVIGSAFSSTYHSYVSSVVARANVLAVSIQYRLAPEHPIPTCFEDCWETVKWITSHSIDGQGQDAWINDHADFSRVFLAGDSAGGTITQNMMVRASSSASCVEDGAVKIVGMVLIHPYFGSGENEKLWEILCSDETGPDDPRLNPMAHLNLLQDLKCSKVLIFIAEKDWIRDRGWIYYETLKKSGWNGHVEIVESLGEDHVFHLMNPNCENANFLMEKIVSFLKD*
>cal_g015614.t1
MDSAPTREAMLSINLTPSNEEVAFEFLPFFRAFKDGRVERFFGTDVVPPTSTEPFPDGVCSKDVQIVAEKKVSGRLFLPSTTKIEPNHQKLPLLIYIHGGAFCFGSPFSQAYHSYLTSVVKEANVVALSIDYRLAPENLLPAAYEDAWDTLKWVVSHADGFGAEPWLNDHADLQRVFVTGDSAGGNISANTVAQAGVQNLAGMKIQGLCLIHPDFASKEGEVYKSWNFACPSSTGGDDPRMNPAKDSRLPNLGCEKVLVCVAGDDPMKERGYYYYDTLKNSGWKGSVEFVETPGESHVFHLFNPTGENALILLRKIAAFMN*
>cal_g015616.t1
MDSIPSDEITVDFFPFFRTYKDGRIHRYNKPTIVPATDDPITGIRSKDVVVSPDNKVSARLFLPGGVEKDEKLPVLVYMHGGAFVIESAFSSQYHSYGSSVAAEAKVLALSVEYRLAPEHPIPACFEDSWAALKWVESHAKDRQGPEPWINNHADFTRIYVGGDSAGGNIAHNTVSRASSEGLEDGMKIVGMILAHPYFGSGQTEKLWDAICSDSDNKGPEDPRLNPMAHPNLLSNLKCHKVLICTGEKDFIRDRGWLYYEALKKSGWSGQVEIFDYEGEGHVFHLLNPKCDNAGIYMKQVVAFLKD*
>cal_g007634.t1
MAISVDYRRVPENPLSAAYEDSWEVLNWVSLHNNSSGEEGSEDWIWDGVDFGKVFLSGDSAGANISHHMAIRAGTESSILEFRGIILIHPYFWGEKPVGLIEIKDPIRKAMVDKWWKFVCPSDKGCDDPLINPFVNNNDSYEICADHSNLGCKRILICVAENDILNERGKMYYRSLIRSKIWKGKAEIVETEGEDHVFHIFNPSCEKAESLVKKCAAFLNQV*
>cal_g008599.t1
MASSSENIATDLSPMLKVYKDGTVKRLYNTTYVPPTSDPDPKTGVSSKDVNITSSISARLFLPSIINSTDQRLPILVYFHGGGFCLESAFSEINHNYINLIVSEAKVLAVSVEYRLAPEHPLPTAYEDCWEAVQWVVSRNNKEPWIINHGNTDKLYIGGDSSGGNIVHNIAMKAGQNHHQEVKILGGFLCCPYFWGSKCKSETDSTINEDGVSLAYRLWMFAYPNADNGIDNPMINPLADDGLSEIGCLRLLMIEGENDVFKETSFTYVQGLKKSGWKGEVELVVVEGEGHCFHLFNLDSEKARCLIKRLASFINA*
>cal_g008598.t1
MGKRREKFEHFNYKQTPSPTVIKPDSCLVHFRLSVLSSHLRSVTDSSEESKFLLGVSSSNCASVAKVIWENECVFCKIVTGQAPALKLTETGLMYVEALKKSRWKGDVDLVDVAGEETDPETAKNLIRRIASFIRK*
>cal_g008602.t1
MVCSNKEVSSEFLPFLQVYKDGSVERFLDSPYVPPSTGSTGDNVSSKDITITGDISARIYLPNSHHHHQLLPVLVYFHGGGFCIESAFSFLSHRYITTLVSHAKILVVSVEYRRAPENPLPAAYQDSWTALQWIASQSVEKTEPWLSNHANFNRVFVGGDSAGANIAHNLAMKASLEDMQNKIHLYGVLLSQPYFLAGSSEKYSNVNDEDEKRLLLYRMWEFVYPNAPGGFDNPLINPFSGDAPSLSWLGGSRLLVCVAEKDELRERGVRYFDAVKKSGWRGKMEFFEVEGEDHCFHIFNLDSQNAQIMIKRLASFLNS*
>cal_g008597.t1
MSSSSSSEDIVSDLSPYIRVYKDGTVERLFSSPFVPPSPNPENGGVSSKDIKISSEVSARVYLPLTDDDEKLPILVYYHGGGFCLESAFSFLDHRYLNLLVSQGKCIAVSVEYRLAPENPLPAAFQDSWTALQWVTSHHHHPAAGVEGDPWLLKHGDFNKLYLGGDSAGGTIVHYLAMRAGVESLPGDVKITGGFLSHPYFWGSKSEQQLINYKKGGAGEVRAGEYNSYPYRMWMLAYPSVPGGIDNPLINPIAEDAPSLLGMTCSRLLVIVAEKDELRETGLMYVEALKKSGGKGDVDLVDVEGEEHCFHIFNTDTEKAENLIRRIASFIRTN*
>cal_g008596.t1
MASSTSSSSEIVTDLSPFIRVYKDGKVERLLGSPFVPACTEHPATGVSSKDVNISPQVSARLYLPLTESIESSEKLPILIYYHGGGFCLDSAFDFLCHRYMNLLVSQAKAVAISVNYRLAPEYPLPAAYEDSWTALTWVASHSVSDIDIGGDKEPWLLNHGNFDKLYLVGDSCGGNIVHNLAIKAGAEILPGDVKIAGGIISHPFFWGSKFESSRTAETTSSDQPPHNHTVGTYYYRLWLFAYPSAPGGIDSPMVNPFPLFSTGEEADNTPPPPPSLSGIGCRKLLVCTSEKDDIRETSVLYFEALKKSGWKGEVEFVDIEGEGHCFQIFKPDTENAKNFISRMASFINS*
>cal_g009982.t1
MRKRRMAAMTLDPSLGLQVNKEHQKHKVNVVEEIDGLIRVYKDGYVERPQIIPTVHSMVAPEPGVTSRDVIIDKGNNIWARFYIPLFQLNMMMMPLIVYFRGGGFCVGSASWICYHEFLAKLAAKSKCVIMSVNYRLAPENPLPAAYDDGLKALFWVKENALTGANEWFSSKCNFASIFLAGDSAGGNIAYSIMSSSRFKLSSSFKPLNFKGAILIQPFFGGETRTHSEKYMVQPPGSALTIAASDTYWRLSLPKGANRDHPWCNPIGKSSPKLENLELLPTLICISEMDILKDRNLELFSGLVSAGHKVECMMYKGVGHAFQILNKSQISQERTEELICHIKNFIFR*
>cal_g015221.t1
MADIRIPGVVKAIDAAANPPYEVDECRGVLRVFSDGSILRSSNPSFIVPVSDDGSVLWKDVVFDATQNLRLRLYKPAAASPISAKLPIFYYIHGGGFCIGSRTWPNCQNYCFKLALDLQAVIISPDYRLAPENRLPAAIEDGFAAVKWLQSQAASEDPDRWLTDVADFGRVFISGDSAGGNIAHNLAVMLGAGSPGLAPVKVKGYVLLAPFFGGTVLSKAEAEGPKDAFLNWELMDRFWRLSVPDGETTDHPLINPFGPVSPSMENLDLDPILVVVGGSDLLKDRADDYAKRLKIWGKKVEYVEFEGQQHGFFTINPNSEPSLELMEVIKKFISKYSA*
>cal_g015615.t1
MATNCSDILHDFFPLLRVYKDGRVERLREQQFVPASVDQETGVECKDVKISKEFDVSARLYLPRNSKPGDKIPLLVYFHGGGFVIESAFSPEYQNHLNLLVREANVLMVSVDYRLAPEHPLPSAYEDSWLVLQWIASHSGGKSSDSEYEAWIKDYADFNRVYFGGDSAGGNIAHNMAMKVGLEGLDGVNLVGIFLNCPYFWGKDPIGDETSNAEIKKYLDNLWLFVYPKTTGLDDPLLNPAADPSLSKLGCKRVLIFVAGQDPLRHRGRHYKEALEKSGWGGIAEVMEFEGEFHVFNLSSPICEKALSMLKTLASFLNQSGL*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University