Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Calotropis gigantea
Species name
Mudar (Calotropis gigantea) is a species of eudicot in the family Apocynaceae (dogbane family).
Species info
Gene sequence info
Calotropis_gigantea.ROMT.fa
Download
>cal_g009234.t1
MAVSDKSSELLKAEAHIWNQTFSFKNSACLKCAVQLGIADAIEKHGKPMSLSELTAALPIHPSKSPYLYRLMRILVNSAFFTDGITGYALTPAARLLLQDDPLNARDFVLLVLDPVMVKPWNALSDWFQNGIDDDNEKAPFDTAHGKNFWDYTAEEPRLGKLFNDAMASDSNLITNVLMQDCKFVFEGLTSLVDVGGGTGTVARAIVKAFPKLNCTVFDLPHVVANQESMKNLDFVAGDMLEKIPSANSILLKWILHNWSDEDCVKILRNCKKAIPAKENGGKVIIIDMKEPRKNGHLFSAKLVSAAARYFPFWVKKSP*
>cal_g009015.t1
MVDPVHRSAFHYLNEWFRNDDPSAFHTAQGMSYWDYAGQNPDFNDLFNLAMARNTEVVSEALARKWEHVFEGLTSVVDVGDAIPNRDKGGKVIIIELNGEADDEEKEKEGGVLWSTVRALHDLQMMAIHGGKERSKKDWKSIFLAAGFSTDYRLSFRAIACGLLCRSILD*
>cal_g019126.t1
MNGSCDDRDHDQDQELLQAQAHIWNHIFNFINSMSLKCAVELGIPDVIHNLNLQHSGGKPATVSELIEALKIDKAKTPFVSRLMRILTHSGFFVRIKTVGSEKEEAAYALTSASKLLLKENPLSVSPFLLAMLDPILTDPWHRLSDWFRKDDLNPFVTAHGKQLWEMADQEPRLNQFFNKAMASDCQFVSSLITGNNNNNRVFEGLNSLVDVGGGTGTMAKVIAKVFPEMKCTVLDLPHVVQGLEGTKNLSYVGGDMFQFIPPSDAILLKWILHDWSDEECVKILKKCKEAIPNKEKGGKVIIIDMVIGDEEKNSGGYDSGATTETKLFFDMLMMVLVTGSERQEKHWAKLFFQAGFSDYKITPILGLRSLIEVYY*
>cal_g019125.t1
MNASCDENDQDQELLQAQAHIWNHIFNFINSMSLKCAVELGIPDAIHNLHGDGKPATVSELIEALKIDKAKTPNGVSAHANTDSLGFEASTERKLMRPKNGLAGDSARMNRLFTSSDAVLLCFG*
>cal_g011562.t1
MDALAKSGNWSGRSDHMAEDSRADLRTRAPFVKEYFNPPTFVSPLIQADKRVTGKGYIIQLKIRAMNYTNHHEVKSAASDEEELLQVQAQAHIWNHTFSFINSMSLKCAVQRGIPDIIHNHGKPMTLSQLMDALKLDSAKAPCMHHLMRMLIHSGFFLTTKMAENDDDENRGGLYSNF*
>cal_g019124.t1
MNKEAVMMNGGYDQDQELLQAQAHIWNHIFSFINSMSLKCAVELGIPDVIHYLYLRGGGNPATVSEIIDALKLDKAKAPFLSRLMRILTHSGFFIRAKKINVDGNDEKEKEEEEEEGYILTSASKLLLKDNPLSLSPFLHALLDPIMTEPWHRLSDWFRSHHDTSPFVTNHGKEFWEMAGQEPRFNNFFNEAMASDCRLVSSLIIGNNEQVFEGLSSLVDVGGGTGNLSKAIAKTFPEMKCTVMDLPQVVQGLKGTKNLSYVGGDMFQAIPPAHAILLKSILHDWSDEESIKILKKCKEVILSREKGGKIIIIDMVLGEDNHEITETKLSSDMFMMVLVTGRERQEKDWKKIFMKAGFSDYKITPILGLRSLIELYY*
>cal_g013844.t1
MMNTNLVVKSEASPSDDDDEEILQAQAHLWNHTVSFINSMSLKCSVELGIPDIIYNHGKPITLSQLVDALKLDSAKAPFIHRLMNMLIPSVASLLSQIWQEMMRKRKRKLIIFSFLFLSFFSKIAHEVSARYCYPSLIQSRQRHGSSA*
>cal_g011560.t1
MNTNHQMVKSEAFDDEELLQAQAHIWNHTFSFINSTSLKCAVQLGIPDIIHSHGKPMTLSQLMDALKLDSAKAPCMHRLLRMLIHSGFFVTTKMIGNDEKEEEKEAYILSSFASKLLLKDSPWSFRSFLLFQLDPIMTAPWQCLADWFRSNDLHNSFVAAHGKSFWEIAEQEQRLNHFFNEAMASDSRLVSSLIVENNNYKRVFEGLSSLVDVGGGTGNMAKAIAKVSPAIKCTVLDLPRIVQGLEGTKNLSYFGGDMFHFIPPSDAVLLKWILYDWSDEDCVKILKKCKEAIPSKEKGGKMMIIEIVLGDESKIEDDSKATETQLFFNMQMIVMVNGKERNEKDWAKLFFQAGFSSYKITLLLGLRSLIEVYYH*
MAVSDKSSELLKAEAHIWNQTFSFKNSACLKCAVQLGIADAIEKHGKPMSLSELTAALPIHPSKSPYLYRLMRILVNSAFFTDGITGYALTPAARLLLQDDPLNARDFVLLVLDPVMVKPWNALSDWFQNGIDDDNEKAPFDTAHGKNFWDYTAEEPRLGKLFNDAMASDSNLITNVLMQDCKFVFEGLTSLVDVGGGTGTVARAIVKAFPKLNCTVFDLPHVVANQESMKNLDFVAGDMLEKIPSANSILLKWILHNWSDEDCVKILRNCKKAIPAKENGGKVIIIDMKEPRKNGHLFSAKLVSAAARYFPFWVKKSP*
>cal_g009015.t1
MVDPVHRSAFHYLNEWFRNDDPSAFHTAQGMSYWDYAGQNPDFNDLFNLAMARNTEVVSEALARKWEHVFEGLTSVVDVGDAIPNRDKGGKVIIIELNGEADDEEKEKEGGVLWSTVRALHDLQMMAIHGGKERSKKDWKSIFLAAGFSTDYRLSFRAIACGLLCRSILD*
>cal_g019126.t1
MNGSCDDRDHDQDQELLQAQAHIWNHIFNFINSMSLKCAVELGIPDVIHNLNLQHSGGKPATVSELIEALKIDKAKTPFVSRLMRILTHSGFFVRIKTVGSEKEEAAYALTSASKLLLKENPLSVSPFLLAMLDPILTDPWHRLSDWFRKDDLNPFVTAHGKQLWEMADQEPRLNQFFNKAMASDCQFVSSLITGNNNNNRVFEGLNSLVDVGGGTGTMAKVIAKVFPEMKCTVLDLPHVVQGLEGTKNLSYVGGDMFQFIPPSDAILLKWILHDWSDEECVKILKKCKEAIPNKEKGGKVIIIDMVIGDEEKNSGGYDSGATTETKLFFDMLMMVLVTGSERQEKHWAKLFFQAGFSDYKITPILGLRSLIEVYY*
>cal_g019125.t1
MNASCDENDQDQELLQAQAHIWNHIFNFINSMSLKCAVELGIPDAIHNLHGDGKPATVSELIEALKIDKAKTPNGVSAHANTDSLGFEASTERKLMRPKNGLAGDSARMNRLFTSSDAVLLCFG*
>cal_g011562.t1
MDALAKSGNWSGRSDHMAEDSRADLRTRAPFVKEYFNPPTFVSPLIQADKRVTGKGYIIQLKIRAMNYTNHHEVKSAASDEEELLQVQAQAHIWNHTFSFINSMSLKCAVQRGIPDIIHNHGKPMTLSQLMDALKLDSAKAPCMHHLMRMLIHSGFFLTTKMAENDDDENRGGLYSNF*
>cal_g019124.t1
MNKEAVMMNGGYDQDQELLQAQAHIWNHIFSFINSMSLKCAVELGIPDVIHYLYLRGGGNPATVSEIIDALKLDKAKAPFLSRLMRILTHSGFFIRAKKINVDGNDEKEKEEEEEEGYILTSASKLLLKDNPLSLSPFLHALLDPIMTEPWHRLSDWFRSHHDTSPFVTNHGKEFWEMAGQEPRFNNFFNEAMASDCRLVSSLIIGNNEQVFEGLSSLVDVGGGTGNLSKAIAKTFPEMKCTVMDLPQVVQGLKGTKNLSYVGGDMFQAIPPAHAILLKSILHDWSDEESIKILKKCKEVILSREKGGKIIIIDMVLGEDNHEITETKLSSDMFMMVLVTGRERQEKDWKKIFMKAGFSDYKITPILGLRSLIELYY*
>cal_g013844.t1
MMNTNLVVKSEASPSDDDDEEILQAQAHLWNHTVSFINSMSLKCSVELGIPDIIYNHGKPITLSQLVDALKLDSAKAPFIHRLMNMLIPSVASLLSQIWQEMMRKRKRKLIIFSFLFLSFFSKIAHEVSARYCYPSLIQSRQRHGSSA*
>cal_g011560.t1
MNTNHQMVKSEAFDDEELLQAQAHIWNHTFSFINSTSLKCAVQLGIPDIIHSHGKPMTLSQLMDALKLDSAKAPCMHRLLRMLIHSGFFVTTKMIGNDEKEEEKEAYILSSFASKLLLKDSPWSFRSFLLFQLDPIMTAPWQCLADWFRSNDLHNSFVAAHGKSFWEIAEQEQRLNHFFNEAMASDSRLVSSLIVENNNYKRVFEGLSSLVDVGGGTGNMAKAIAKVSPAIKCTVLDLPRIVQGLEGTKNLSYFGGDMFHFIPPSDAVLLKWILYDWSDEDCVKILKKCKEAIPSKEKGGKMMIIEIVLGDESKIEDDSKATETQLFFNMQMIVMVNGKERNEKDWAKLFFQAGFSSYKITLLLGLRSLIEVYYH*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University