Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Calotropis gigantea
Species name
Mudar (Calotropis gigantea) is a species of eudicot in the family Apocynaceae (dogbane family).
Species info
Gene sequence info
Calotropis_gigantea.4CL.fa
Download
>cal_g014431.t1
MDTNKGQQESIFRSKLPDIYIPNHLPLHTYCFENISQFSNRPCLINGSTGDIYTYSDVELAVRKFASGLHKLGIQRGDVIMLLLHNSPEFAFAFLGASHLGAISTTANPFCTTFEIAKQVKASKAKLIVTQSCYIEKVKDLIGKNNDHVIKIVVVAADSKDKTNYLYFSELMSKGNENEIPAVKINPGDDVVALPYSSGTTGFPKGVMLTHKGLITSIAQQVDGENPNLYLHKEDVIICVLPLFHIYSLNSVLLCGLRVGATILIMPKFEIIPLMELVQKYKVTIAPFVPPIVLAIAKCPVTEKYDLSSIRMLMSGAAPMGRELEDTVKAKLPNAILGQGYGMTEAGPVLSMCLAFAKEPFKVKSGACGTVVRNAEMKIMDHQTGLSLPRNQPGEICIRGNQIMKGYLNDLEATERTIDKEGWLHTGDIGYIDNDDEVFIVDRLKELIKYKGFQVAPAELEAILISHPSISDAAVVPYTPTPRTLSADRSASICRRVNF*
>cal_g004973.t1
MAIKTKEEEMIFRSKLPDIYIPNHLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELTSRKVAAGLSKLGVRKGDTIMILLPNSPEFIFSFLGASYLGAISTMANPLFTPAEVVKQAKASSAKLIITQASYVEKVKDFACGGAIKIVCTDSTPAGEEEYCLHFSELKNADEKDMPDDVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSDDVLMCVLPLFHIYSLNSILLCGLRAGSAILIMQKFSIVPFLELIQKYKVTIGPFVPPIVLEIAKSPVVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNARLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETGSALPRNQPGEICIRGDQIMRGYLNDPEATEKTIDKEGWLHTGDVGYIDEDDELFIVDRLKELIKYKGFQVAPAELEALLITHPFIKDAAVVPMKDDAAGEVPVAFVVRSSPNGGGDDSTIITEDEIKEFISKQVIFYKRIKRVFFVEVIPKSPSGKILRKDLRARLAAGVPN*
>cal_g017462.t1
MLSVEAPTNKELSVPVSMETASSGSPNKQEVTLREPGHIFRSKLPEILISNHLPLHAYCFEKLSEYPDRTCLIVGSNGNTYTFAETHLMCRKVAAGLSSLGVKKGEVIMILLQNSAEFVFSFMGASMIGAVVTTANPFYTSAEVFKQFKAAKAKLIITQSHYVQKLRDAPDDCPKLGEDDEFQVITVDDPPENCQHFSVLSEGNENEVPSVNIAPDDPVALPFSSGTTGLPKGVILTHKSLVTSVGQQVDGENPNLYLKSDDVVLCVLPLFHIYSLNSVLLCSLRAGASVLLLQKFEMVSLLELIQKHRVSMAAVVPPLVLALAKNPMVDSYDLSSIRLVLSGAAPLGKELEEALHSRLPQATFGQGYGMTEAGPVLSMSPSFAKQPLPSKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGPQIMKGYLNDDQATARTIDSDGWLHTGDIGLVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLLTHPQIEDAAVVPQKDNVAGEVPVAFVVPSIESELTEEAVKDFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLPAATVS*
>cal_g002174.t1
MATTNTRSLPEDENIFRSRYPQVQVPDMSLPDFVLRDAESYSPNVAFVDAATGKTYTYGEVSRDVRRFARALRSLGVRTGHVVLVVLPNFVEYAVIALGIMAAGGVFSCANPDSHSSEINKQVDIAEAKLIVTDASTHRKVKDSGVPVIVVSEEAIEGSINWEKLL*
>cal_g006622.t1
MEKSGYGRDGIYRSLRPPLVLPKPTSNISMVSFFFRNISSYPDKPALIDGHTGQTLSFSELKSMVSKVSFGLYHQLGIKKNDVVLIFAPNSIQFPLCFLSIIAVGGIATTANPIYTVSELSKQVKDCAPKLIITVPELIPKVSGFNLPLLLLGCSSEPSKFSGEVVIFSDLIKNEGSLDWDYFTRNTKQSDTAALLYSSGTTGVSKGVILTHRNFIASSLMITADQEFAGEMHNLYLCVLPMFHVFGLAVIIFSQLQRGNAVVSMGKFDLEMILKAVEKYRITHLWVVPPIVLALAKNSAVKKYDLSSLKQIGSGAAPLGRDLMLDCAKNFPQAVVIQGYGMTETCGIVSVENPNLCSRHSGSAGMLVPGVEAEIVSVEKLKPLPPGQLGEIWVRGQNMMQGYFQNPEATKLTIDKQGWVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLISHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEVDVQKFIADQVAPFKRLRRVTFINSVPKSASGKILRRELIGKVRSKL*
>cal_g002173.t1
MLTHRNIVANLCSSLFSVGPELIGKVIILGLIPFFHIYGLTGICCATLRNKGKVVVLPRYELRAFLGALIKHEVNFAPIVPPIILGLVKNPIVDEFDLNKLKLRTVMTAAAPLAPELLNEFEKKFPGVQVQEAYGMTEHSCITLSHGDPSKGHGIAKRNSVGFILPNLEVKFVDPETGSSLPKNTPGEICVRSQCVMKGYYKNEYETAMTIDKDGWLHTGDVGYIDDDGDVFIVDRIKEIIKYKGFQVAPAEIEGVLLTHPSVEDAAVVGLPDEAAGEIPAACVVINQDSEETEEEIMHFVASNVAHYKKVRKVQFVDAIPKSHSGKIMRRLVKEIMAENAKLNLRSVAI*
MDTNKGQQESIFRSKLPDIYIPNHLPLHTYCFENISQFSNRPCLINGSTGDIYTYSDVELAVRKFASGLHKLGIQRGDVIMLLLHNSPEFAFAFLGASHLGAISTTANPFCTTFEIAKQVKASKAKLIVTQSCYIEKVKDLIGKNNDHVIKIVVVAADSKDKTNYLYFSELMSKGNENEIPAVKINPGDDVVALPYSSGTTGFPKGVMLTHKGLITSIAQQVDGENPNLYLHKEDVIICVLPLFHIYSLNSVLLCGLRVGATILIMPKFEIIPLMELVQKYKVTIAPFVPPIVLAIAKCPVTEKYDLSSIRMLMSGAAPMGRELEDTVKAKLPNAILGQGYGMTEAGPVLSMCLAFAKEPFKVKSGACGTVVRNAEMKIMDHQTGLSLPRNQPGEICIRGNQIMKGYLNDLEATERTIDKEGWLHTGDIGYIDNDDEVFIVDRLKELIKYKGFQVAPAELEAILISHPSISDAAVVPYTPTPRTLSADRSASICRRVNF*
>cal_g004973.t1
MAIKTKEEEMIFRSKLPDIYIPNHLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELTSRKVAAGLSKLGVRKGDTIMILLPNSPEFIFSFLGASYLGAISTMANPLFTPAEVVKQAKASSAKLIITQASYVEKVKDFACGGAIKIVCTDSTPAGEEEYCLHFSELKNADEKDMPDDVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSDDVLMCVLPLFHIYSLNSILLCGLRAGSAILIMQKFSIVPFLELIQKYKVTIGPFVPPIVLEIAKSPVVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNARLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETGSALPRNQPGEICIRGDQIMRGYLNDPEATEKTIDKEGWLHTGDVGYIDEDDELFIVDRLKELIKYKGFQVAPAELEALLITHPFIKDAAVVPMKDDAAGEVPVAFVVRSSPNGGGDDSTIITEDEIKEFISKQVIFYKRIKRVFFVEVIPKSPSGKILRKDLRARLAAGVPN*
>cal_g017462.t1
MLSVEAPTNKELSVPVSMETASSGSPNKQEVTLREPGHIFRSKLPEILISNHLPLHAYCFEKLSEYPDRTCLIVGSNGNTYTFAETHLMCRKVAAGLSSLGVKKGEVIMILLQNSAEFVFSFMGASMIGAVVTTANPFYTSAEVFKQFKAAKAKLIITQSHYVQKLRDAPDDCPKLGEDDEFQVITVDDPPENCQHFSVLSEGNENEVPSVNIAPDDPVALPFSSGTTGLPKGVILTHKSLVTSVGQQVDGENPNLYLKSDDVVLCVLPLFHIYSLNSVLLCSLRAGASVLLLQKFEMVSLLELIQKHRVSMAAVVPPLVLALAKNPMVDSYDLSSIRLVLSGAAPLGKELEEALHSRLPQATFGQGYGMTEAGPVLSMSPSFAKQPLPSKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGPQIMKGYLNDDQATARTIDSDGWLHTGDIGLVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLLTHPQIEDAAVVPQKDNVAGEVPVAFVVPSIESELTEEAVKDFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLPAATVS*
>cal_g002174.t1
MATTNTRSLPEDENIFRSRYPQVQVPDMSLPDFVLRDAESYSPNVAFVDAATGKTYTYGEVSRDVRRFARALRSLGVRTGHVVLVVLPNFVEYAVIALGIMAAGGVFSCANPDSHSSEINKQVDIAEAKLIVTDASTHRKVKDSGVPVIVVSEEAIEGSINWEKLL*
>cal_g006622.t1
MEKSGYGRDGIYRSLRPPLVLPKPTSNISMVSFFFRNISSYPDKPALIDGHTGQTLSFSELKSMVSKVSFGLYHQLGIKKNDVVLIFAPNSIQFPLCFLSIIAVGGIATTANPIYTVSELSKQVKDCAPKLIITVPELIPKVSGFNLPLLLLGCSSEPSKFSGEVVIFSDLIKNEGSLDWDYFTRNTKQSDTAALLYSSGTTGVSKGVILTHRNFIASSLMITADQEFAGEMHNLYLCVLPMFHVFGLAVIIFSQLQRGNAVVSMGKFDLEMILKAVEKYRITHLWVVPPIVLALAKNSAVKKYDLSSLKQIGSGAAPLGRDLMLDCAKNFPQAVVIQGYGMTETCGIVSVENPNLCSRHSGSAGMLVPGVEAEIVSVEKLKPLPPGQLGEIWVRGQNMMQGYFQNPEATKLTIDKQGWVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLISHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLTEVDVQKFIADQVAPFKRLRRVTFINSVPKSASGKILRRELIGKVRSKL*
>cal_g002173.t1
MLTHRNIVANLCSSLFSVGPELIGKVIILGLIPFFHIYGLTGICCATLRNKGKVVVLPRYELRAFLGALIKHEVNFAPIVPPIILGLVKNPIVDEFDLNKLKLRTVMTAAAPLAPELLNEFEKKFPGVQVQEAYGMTEHSCITLSHGDPSKGHGIAKRNSVGFILPNLEVKFVDPETGSSLPKNTPGEICVRSQCVMKGYYKNEYETAMTIDKDGWLHTGDVGYIDDDGDVFIVDRIKEIIKYKGFQVAPAEIEGVLLTHPSVEDAAVVGLPDEAAGEIPAACVVINQDSEETEEEIMHFVASNVAHYKKVRKVQFVDAIPKSHSGKIMRRLVKEIMAENAKLNLRSVAI*
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University