Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Calamus simplicifolius
Species name
Calamus simplicifolius is a species of monocot in the family Arecaceae (palm family).
Species info
Gene sequence info
Calamus_simplicifolius.ANR.fa
Download
>Maker00007080
MTTYSDEGRVGLSVECSRLQVAKLLQDLKHEDWREDPLCPAPVIQNGVPRRSRSGSTPLAALALPDSARGLLIGVCLGVGLLPLTAPHPNSWLPCWLLCYRGSSAAGSSATGSSVGTPSVTALSGGMSAVRSISRATGGSCGTGGSGTCTVGRFGAGARRSSLGPSRALFLATALRSFWVETFMAVQLWSGSVELIDPAVKGTLNVFSSCIKTPTVKRVVVTSSMAAVVFDGRPRTPDVIVDETWFSRAEV
>Maker00022128
MERRSKVCVTGASGYLGSWLVKKLLKNGYVVHATLRNLEDKSKTQLLKSFPGANTRMHLFEAELYNPLTFEAAIQGCEFVFLLATPLQHNANSQYKDTTEAAVAAVRSMLQLCERSGTVKRVIYTGSVTAASPLKEDGTGFKDFIDESHWTPLHLAFAYCEDFEKSYICSKTESEKEVLSYNVYKEGKKEFEVVSLACALVGGAAVLPAAPLSIQCMVSPLTGDKVKHRQMKFLQALLGSVPLVHIEDVCEANIFCMEKPSMAGRFLLASAYPTMQEIVDYYTQNYPEFRVIKEVEGDGHGIGCRSTKLADMGFKFRYDVKQILDGSVETAKRLGCLDELA
>Maker00022132
MERRSKVCVTGASGFLASWLVKELLKKGHVVHATLRNLEDKSKTQLLKSFPGADTRLHLFEAELYNPLTFEAAIQGSEFVFLLATPMQHNANSSQYKDTTQAAVAAVRSMLQLCERSGTVKRVIYTGSVTAASPFKEDGTSFKDFIDESCWTPLHPTFAYCEDFEKSYICSKTESEKEVLSYNVYKEGKKEFEVVSLACALVGGDTVLPVAPLTLQCMVSPLTGDKVKHRQMKFLQALLGSVPLVHIEDVCEAHIFCMEKPSMAGRFLLASAYPTMQEIVDYYTQNYPEFRVIKEVEGDGHGIGCRSTKLADMGFKFRYDVKQILDGSVETAKRLGCLDELA
>Maker00023742
MSKEGRVCVTGGSGFIGSWVVRLLLEHGYTVHATVKDLNDEGETKHLRGLDGASPRLHLFQMDLLDYNSILTAIQGTTGVFHLASPTMIDAVQDLQALLHLFIDLCFASVLIFATDKLVGEQRELLDPAVKGTLNVLRAAKESRVRRVVVTSSSAAIVPNPSWPAGVVYNEDSLTDVEHCKKIELWYPVSKTLAERAAWEFALNEGLDVVVINPGMVLGPVLPPAIGLSVAILLHLLQGKEIEGENSHLGCVDVRDVALAHILLYEKPMAQGRHLCVEAITHWSDFVAQVAELCPDFPVPRHLEDKQPWLVRADSPSKKLIELGLGFRPLEETIKDTLDRDVWCNDDDENINYSSLNRGSKEEEDGRLEMSNQGRVCVTGGSGFIGSWVIRLLLERGYTVHATVKDLNDEGETKHLRGLDGASSRLRLFQIDLLDYNSILAAIQGTTGVFHLASPNVIDPRELLDPAVKGTLNMLRAAKESRVRRVIVTSSSAAIFPNPSWPAGIAYNEDSWTDVEHCKKIEVLAGDLSYSMALGKAVELENVHLGCVDVRDAALAHLLLYEKPLAQGRHLCVEAITRWSDTIAKVAELCPEFPVPRHLEDKQPWLVRAESPSKKLMELGLRFRSMEEMIKDAVDSLKSKGYI
>Maker00049195
MAEKGTVFVTGAAGFVGSWLIMRLLEQGYTVKATVRDPSYVFNMTKKGTICVTGAAGFVGSWLIMRLLEQGYTVKETNEVIKPTIDGALNILKSCVKARTVKRVVFTSSAGTVVMHENRKSVYEEDSWSDVDFAGPRR
>Maker00008541
MPEYCVTGGTGFIAGYIVKELLARGHTVRATVRDPDDETKVGFLWSLDGAKERLKLVKADLLVEGSFDDAVNGADGVFHAACPVFVSQDQNIEEKLINPAIKGTANVLKSCSKAGSVRRVVLTSSCSSIRYRDDATQASPLNESSWSDVEYCKRYNLWYAYAKTLAEREAWKLAKDFRIDLVVVNPSFVVGPLLAPQPTSTLYVILSLLKGACPNYPNTTIGFVHIDDVVLGHILAMEDSRASGRLVCSSSVAHWSEIVEMLKPNSMLSNPHQQGDSNPHSMDTTKIRNWGFHRSKLFPRCLMTALRVSKRRNFCEPSTARTHITDREGAKRKSVRVGGIMPEYCVTGGTGLLAAHLIRSLLAEGHTVRATVRDPGDEAKVGFLWHLDGAKERLRLLKADLMVEGSFDEAVDGADGVFHVASPIFVPPDQNLQETLIDPAVRGTANVLRSCSKASSVRRVVLTSSSTAVRCRPDATLVSPLNESHWSDLEYCKRYDLWYSYAKVLAEQEAWRLAKEYGIDLVVVIPSFVVGPLLTPQPRSTLQMILSIVKGDRPNYPNRTAGFVHIDDVVLGHILAMEEPRASGRLICSADVAHWSEIMEMLRAKYPTYPIPNKCGNEKGVSIPHTWIPLRSGS
>Maker00032609
MGNALKYIPSSAAASSPVTTHHIVFVSIYFLQGSTVLEMDGSDPPSSSSAAAAAAAAKTTVCVTGATGYVGSWLVRSLLQRGYSVHATARDPGKAWCLLSTFSGNSQLKIFRADLNEEGSFDEAVKGCFGVFHVAASMEFDIPVEENIGKLPLVCLSINEEDYARSKIIEPAVRGTLNVLQACSRAGSVKRIIFTSSVSTITAKDNKGEWRSLVDESSVTPVNVVWNTSLKDGKQHMLYIVINLQHLNLQLPLYVKLEMTFRLVYVLSKLLTEEKAFQFAKEKGMDLVSIIPPTVAGPFLTTSVPASVRVLLSPVTGDPNFYPILVSVHSRLGAIPLVHIEDICSAHIFLMEQAAAEGRFICSVGSSTMPQLTDLLSLEYPSFSSERFDKRFHNAIPSVISSKRLTDLGFKFKYGVRDIIEQSVACCIKHGFLKCPLTEGICN
>Maker00037348
MPEYCVTGGTGFIASYLVRALLAKGHVVRATVRDPGDETKVGFLWSFDGAKERLRLVKANLLVEGSFDEAVDGADGVFHIATPVYAPQGQNIQEAMIDPAIRGTANVLKSCSKTSSVRRVVLTSSTAAVGYRYDATAASPLNESHWSDLEYCKRYNMWYAYAKTQAEKEAWQLAKEYGIDLVVVLPAFVVGPLLTPHPQALSKGHYRSLKVKHISPNPRLILIILTKRNHKCRTRLLILFIKNRSAGEHPVYPNRIGGFVHIDDVVLGHILAMEESRASGRLICSGGVANWSDIVGC
>Maker00035743
MTTEGKPACVTGGTGFMASALIKQLLEKGYANELIKPAVQGILNVMKSCLKANSVKRFVLTSSAAAVSIKQPTKKNLMLDEESWSDMEFLTANKLPTCAYGVSKMFTEREALRFAQENNITLVTMIPALRIVLH
>Maker00040577
MAEVGEKRKVCVTGAGGYVASWLVKLLLSKGFIVHGTVRDPSTAPFSLLCTTFPLFFHLSAAFGDAFLGSSHSLTGGDGRNAHLQKLEKASENLKLFKADLLDYNSIASAIAGCEGVEVITPAVTGTLNVLKACSEAKVKRVVVVSSAAAVCMIPNWPTDKAMDENCWSDKEYCRTTEVIDIMCLSYPHASHMKFVFFLM
>Maker00040581
MRDRSGRVRSLMAGEATPLQGLYRPRNRDEKNAHLKKLENASENLKLFKADLLDYDAVASAIAGCEGVFHVASPVPFTKAELITPAVTGTLNVLKACSEAEVKRVVVNWYCLSKTLAESEALEYAEKHGLDVVTVCPCMVIGPLLQSTVNASSLVLIHLLKGGYESIENKNRDIVDVRDVADALFMVYEKPKASGRYICSSHFIKVSGLVDMLKRMYPNYSYPKIFVEVDESSRRSSEKLKMLGWKYRTLEESLIDSVECYRDAGLLNKD
>Maker00048630
MAEVGEKRKVCVTGAGGYVASWLVKLLLSKGFIVHGTVRDPSDGRNAHLQKLEKASENLKLFKADLLDYNSIASAIAGCEGVFHVASPVPAIKVPNPEASSPGSYLYFRMFQNTSHTSFQLYMKGLQVEVITPAVTGTLNVLKACSEAKVKRVVVVSSAAAVCMIPNWPTDKAMDENCWSDKEYCRTTENWYCLSKTLAESEALEYAEKHGLDVVTVCPCMVIGPLLQSTVNASSMFLINLLKELVDGIFFNIHPFLYLSSSLLSYCSKSEASGRYICGPHFVKMGDLMDMLKRMYPNYNYPKTFVEGDEDSGLSSEKLKMLGWECRALEETLIDSVECYRDCGLLSKD
>Maker00004811
MNPHKEKPGRRGERERECKRAMEVAGEKGRVCVTGAGGYLASWLVKLLLSNGYKVHGTVRDLSVEKNSYLRKFEKASENLQLFKADLLDYGTLKAAIAGCEGVFHVASPVPATKILDPEASLLYAYIYSGSMFSWKGNAESFSEVISPAVNGTLNILQACSEMKIKRVVVVSSVAAVELNPHWPHDKLKDEECWSNEEFCKQIENWYCLSKTMAEHAAWEYAEKKGLDLVTVCPSLVFGPLLQPTASTSSLFLIYVIKGGPEPMKDDFWHIVDVRDVADALLLVYEKPESSGRYICSAYQNSVQNFVDLLKEMYPNYGYQKNLIEVPRNTPMTSEKLKKLGWRCRPLKETLADAAECYKEAGLLDEEEGHAYLPPLYK
>Maker00005601
MTNNEESLPASRGPLASLQNWEKLSPLREMQNLGSLKIFRADLRDEGSFDEAVAGSDYVFYVAAPVNLHTDDPENELIKPAVQGVLNVMKSCLKAKSVKRFVLTSSAAAVSVKRPAKSSLTLDEESWSDMEFLTIDKPPTWGYGVSKMLTEREALKFAQENNISLVTIIPSLTIGPAPAATFKRCTGIAMALSLLTGNEMLINGLKLMYSFSGSISMVHIEDICRAHIFVAENESASGRYICCSINTTLTELASFLSKLYPQYKVPTDFAGVPEMGKLSLSSEKLIKAGFEFKYKQLEEFYDEIVECAITAGMLPPK
MTTYSDEGRVGLSVECSRLQVAKLLQDLKHEDWREDPLCPAPVIQNGVPRRSRSGSTPLAALALPDSARGLLIGVCLGVGLLPLTAPHPNSWLPCWLLCYRGSSAAGSSATGSSVGTPSVTALSGGMSAVRSISRATGGSCGTGGSGTCTVGRFGAGARRSSLGPSRALFLATALRSFWVETFMAVQLWSGSVELIDPAVKGTLNVFSSCIKTPTVKRVVVTSSMAAVVFDGRPRTPDVIVDETWFSRAEV
>Maker00022128
MERRSKVCVTGASGYLGSWLVKKLLKNGYVVHATLRNLEDKSKTQLLKSFPGANTRMHLFEAELYNPLTFEAAIQGCEFVFLLATPLQHNANSQYKDTTEAAVAAVRSMLQLCERSGTVKRVIYTGSVTAASPLKEDGTGFKDFIDESHWTPLHLAFAYCEDFEKSYICSKTESEKEVLSYNVYKEGKKEFEVVSLACALVGGAAVLPAAPLSIQCMVSPLTGDKVKHRQMKFLQALLGSVPLVHIEDVCEANIFCMEKPSMAGRFLLASAYPTMQEIVDYYTQNYPEFRVIKEVEGDGHGIGCRSTKLADMGFKFRYDVKQILDGSVETAKRLGCLDELA
>Maker00022132
MERRSKVCVTGASGFLASWLVKELLKKGHVVHATLRNLEDKSKTQLLKSFPGADTRLHLFEAELYNPLTFEAAIQGSEFVFLLATPMQHNANSSQYKDTTQAAVAAVRSMLQLCERSGTVKRVIYTGSVTAASPFKEDGTSFKDFIDESCWTPLHPTFAYCEDFEKSYICSKTESEKEVLSYNVYKEGKKEFEVVSLACALVGGDTVLPVAPLTLQCMVSPLTGDKVKHRQMKFLQALLGSVPLVHIEDVCEAHIFCMEKPSMAGRFLLASAYPTMQEIVDYYTQNYPEFRVIKEVEGDGHGIGCRSTKLADMGFKFRYDVKQILDGSVETAKRLGCLDELA
>Maker00023742
MSKEGRVCVTGGSGFIGSWVVRLLLEHGYTVHATVKDLNDEGETKHLRGLDGASPRLHLFQMDLLDYNSILTAIQGTTGVFHLASPTMIDAVQDLQALLHLFIDLCFASVLIFATDKLVGEQRELLDPAVKGTLNVLRAAKESRVRRVVVTSSSAAIVPNPSWPAGVVYNEDSLTDVEHCKKIELWYPVSKTLAERAAWEFALNEGLDVVVINPGMVLGPVLPPAIGLSVAILLHLLQGKEIEGENSHLGCVDVRDVALAHILLYEKPMAQGRHLCVEAITHWSDFVAQVAELCPDFPVPRHLEDKQPWLVRADSPSKKLIELGLGFRPLEETIKDTLDRDVWCNDDDENINYSSLNRGSKEEEDGRLEMSNQGRVCVTGGSGFIGSWVIRLLLERGYTVHATVKDLNDEGETKHLRGLDGASSRLRLFQIDLLDYNSILAAIQGTTGVFHLASPNVIDPRELLDPAVKGTLNMLRAAKESRVRRVIVTSSSAAIFPNPSWPAGIAYNEDSWTDVEHCKKIEVLAGDLSYSMALGKAVELENVHLGCVDVRDAALAHLLLYEKPLAQGRHLCVEAITRWSDTIAKVAELCPEFPVPRHLEDKQPWLVRAESPSKKLMELGLRFRSMEEMIKDAVDSLKSKGYI
>Maker00049195
MAEKGTVFVTGAAGFVGSWLIMRLLEQGYTVKATVRDPSYVFNMTKKGTICVTGAAGFVGSWLIMRLLEQGYTVKETNEVIKPTIDGALNILKSCVKARTVKRVVFTSSAGTVVMHENRKSVYEEDSWSDVDFAGPRR
>Maker00008541
MPEYCVTGGTGFIAGYIVKELLARGHTVRATVRDPDDETKVGFLWSLDGAKERLKLVKADLLVEGSFDDAVNGADGVFHAACPVFVSQDQNIEEKLINPAIKGTANVLKSCSKAGSVRRVVLTSSCSSIRYRDDATQASPLNESSWSDVEYCKRYNLWYAYAKTLAEREAWKLAKDFRIDLVVVNPSFVVGPLLAPQPTSTLYVILSLLKGACPNYPNTTIGFVHIDDVVLGHILAMEDSRASGRLVCSSSVAHWSEIVEMLKPNSMLSNPHQQGDSNPHSMDTTKIRNWGFHRSKLFPRCLMTALRVSKRRNFCEPSTARTHITDREGAKRKSVRVGGIMPEYCVTGGTGLLAAHLIRSLLAEGHTVRATVRDPGDEAKVGFLWHLDGAKERLRLLKADLMVEGSFDEAVDGADGVFHVASPIFVPPDQNLQETLIDPAVRGTANVLRSCSKASSVRRVVLTSSSTAVRCRPDATLVSPLNESHWSDLEYCKRYDLWYSYAKVLAEQEAWRLAKEYGIDLVVVIPSFVVGPLLTPQPRSTLQMILSIVKGDRPNYPNRTAGFVHIDDVVLGHILAMEEPRASGRLICSADVAHWSEIMEMLRAKYPTYPIPNKCGNEKGVSIPHTWIPLRSGS
>Maker00032609
MGNALKYIPSSAAASSPVTTHHIVFVSIYFLQGSTVLEMDGSDPPSSSSAAAAAAAAKTTVCVTGATGYVGSWLVRSLLQRGYSVHATARDPGKAWCLLSTFSGNSQLKIFRADLNEEGSFDEAVKGCFGVFHVAASMEFDIPVEENIGKLPLVCLSINEEDYARSKIIEPAVRGTLNVLQACSRAGSVKRIIFTSSVSTITAKDNKGEWRSLVDESSVTPVNVVWNTSLKDGKQHMLYIVINLQHLNLQLPLYVKLEMTFRLVYVLSKLLTEEKAFQFAKEKGMDLVSIIPPTVAGPFLTTSVPASVRVLLSPVTGDPNFYPILVSVHSRLGAIPLVHIEDICSAHIFLMEQAAAEGRFICSVGSSTMPQLTDLLSLEYPSFSSERFDKRFHNAIPSVISSKRLTDLGFKFKYGVRDIIEQSVACCIKHGFLKCPLTEGICN
>Maker00037348
MPEYCVTGGTGFIASYLVRALLAKGHVVRATVRDPGDETKVGFLWSFDGAKERLRLVKANLLVEGSFDEAVDGADGVFHIATPVYAPQGQNIQEAMIDPAIRGTANVLKSCSKTSSVRRVVLTSSTAAVGYRYDATAASPLNESHWSDLEYCKRYNMWYAYAKTQAEKEAWQLAKEYGIDLVVVLPAFVVGPLLTPHPQALSKGHYRSLKVKHISPNPRLILIILTKRNHKCRTRLLILFIKNRSAGEHPVYPNRIGGFVHIDDVVLGHILAMEESRASGRLICSGGVANWSDIVGC
>Maker00035743
MTTEGKPACVTGGTGFMASALIKQLLEKGYANELIKPAVQGILNVMKSCLKANSVKRFVLTSSAAAVSIKQPTKKNLMLDEESWSDMEFLTANKLPTCAYGVSKMFTEREALRFAQENNITLVTMIPALRIVLH
>Maker00040577
MAEVGEKRKVCVTGAGGYVASWLVKLLLSKGFIVHGTVRDPSTAPFSLLCTTFPLFFHLSAAFGDAFLGSSHSLTGGDGRNAHLQKLEKASENLKLFKADLLDYNSIASAIAGCEGVEVITPAVTGTLNVLKACSEAKVKRVVVVSSAAAVCMIPNWPTDKAMDENCWSDKEYCRTTEVIDIMCLSYPHASHMKFVFFLM
>Maker00040581
MRDRSGRVRSLMAGEATPLQGLYRPRNRDEKNAHLKKLENASENLKLFKADLLDYDAVASAIAGCEGVFHVASPVPFTKAELITPAVTGTLNVLKACSEAEVKRVVVNWYCLSKTLAESEALEYAEKHGLDVVTVCPCMVIGPLLQSTVNASSLVLIHLLKGGYESIENKNRDIVDVRDVADALFMVYEKPKASGRYICSSHFIKVSGLVDMLKRMYPNYSYPKIFVEVDESSRRSSEKLKMLGWKYRTLEESLIDSVECYRDAGLLNKD
>Maker00048630
MAEVGEKRKVCVTGAGGYVASWLVKLLLSKGFIVHGTVRDPSDGRNAHLQKLEKASENLKLFKADLLDYNSIASAIAGCEGVFHVASPVPAIKVPNPEASSPGSYLYFRMFQNTSHTSFQLYMKGLQVEVITPAVTGTLNVLKACSEAKVKRVVVVSSAAAVCMIPNWPTDKAMDENCWSDKEYCRTTENWYCLSKTLAESEALEYAEKHGLDVVTVCPCMVIGPLLQSTVNASSMFLINLLKELVDGIFFNIHPFLYLSSSLLSYCSKSEASGRYICGPHFVKMGDLMDMLKRMYPNYNYPKTFVEGDEDSGLSSEKLKMLGWECRALEETLIDSVECYRDCGLLSKD
>Maker00004811
MNPHKEKPGRRGERERECKRAMEVAGEKGRVCVTGAGGYLASWLVKLLLSNGYKVHGTVRDLSVEKNSYLRKFEKASENLQLFKADLLDYGTLKAAIAGCEGVFHVASPVPATKILDPEASLLYAYIYSGSMFSWKGNAESFSEVISPAVNGTLNILQACSEMKIKRVVVVSSVAAVELNPHWPHDKLKDEECWSNEEFCKQIENWYCLSKTMAEHAAWEYAEKKGLDLVTVCPSLVFGPLLQPTASTSSLFLIYVIKGGPEPMKDDFWHIVDVRDVADALLLVYEKPESSGRYICSAYQNSVQNFVDLLKEMYPNYGYQKNLIEVPRNTPMTSEKLKKLGWRCRPLKETLADAAECYKEAGLLDEEEGHAYLPPLYK
>Maker00005601
MTNNEESLPASRGPLASLQNWEKLSPLREMQNLGSLKIFRADLRDEGSFDEAVAGSDYVFYVAAPVNLHTDDPENELIKPAVQGVLNVMKSCLKAKSVKRFVLTSSAAAVSVKRPAKSSLTLDEESWSDMEFLTIDKPPTWGYGVSKMLTEREALKFAQENNISLVTIIPSLTIGPAPAATFKRCTGIAMALSLLTGNEMLINGLKLMYSFSGSISMVHIEDICRAHIFVAENESASGRYICCSINTTLTELASFLSKLYPQYKVPTDFAGVPEMGKLSLSSEKLIKAGFEFKYKQLEEFYDEIVECAITAGMLPPK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University