Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Cakile maritima
Species name
European sea-rocket (Cakile maritima) is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Cakile_maritima.FLS.fa
Download
>Camar.0037s0039.1.v1.1
MEVERVHYTSPPSETVPIVDLSDPDDELVARTLVKASEEWGAFQVVNHGIPAELMRRFQDVGRQFFELPTAEKEAVAMRPGSKSIEGYDARNPKLAKSWDDHLLHKIWPQSCIDYRFWPKNPPDYREVTEEYASHVKKLSEKIMGYLSKGLGLELETLKERLGGGEYMLRINYYPPSDSVIGAPAHTDFDALALLVSNGVPGLQVFKDSHWLDVEDINSAIVIILGDQMMRLSNGRYKSVLHRSTMDKEKTRMTWPVLIDPMPGMVVGPLPEIIGDENPPKFESLTLEEYIYRRTNMLLGDG
>Camar.0037s0038.1.v1.1
NLDKELVAAAVVKASQEWGVFQIVNHEIPEELIKNLKKVGREFFELSSEAEKEAVATPANSKDIQGYVKTSHKDVEDKVDRVDHLFHNLSPPSWIDYRYWPKNPPEYRQVNEEYAVYVKKLAEKILGWLSEGVGLDREALFNKGFGGESAIALYNLFCSSLVSNIICFKKTESYIYLTKAKRMSNGKYKNVLHRTTLNKEKTRMSWAVHLKPAYDMVIEPVMKLTSHGDEAPKFNPITYGELVYRRVNNLPLD
>Camar.0037s0037.1.v1.1
MMDKEKTRMTWQVLIDPMPGMVVGPLPELIGDENPPNLLLNLSATKISPCSSLLIITNTSRSVNSLPTEYELSELERNQNTFRGTSMDVPIIDLSNSDEELVARAVVKASEEWGIFQVVNHEIPVELIRRLKEVGTEFFELPETEKELVAKPTDSMDLEGYITKFRKDLEGRDAWIDHLFHRIWPPSRVTYRFWPKNPPEYREVNEEYARHIKKLSEKIMKWLSEGLGLRREALKEGLGGETVEYLMKINYYPQCPDQDLVVGAPAHTDVNGITFLVANEASGLQALKDNHWIDTTCTTSGIFVIIGDQFRMMSNGKYKGAPHRATMVKEKTRISWPVFVESSLDHEFGPLPELLTGEDNAPKFKPIVYKEHKFRKPKNLVLD
>Camar.0037s0040.1.v1.1
MEVETVPIIDLSGDLDDELVTHAMVKASEEWGVFQVVNHGIPAELMRRLQEVGRQFFELPAEEKEAVATLPPGSKSLQGYGAKNPKLGQSWNDHLFHKIWPQSCVDYRFWPKNPSDYR
>Camar.0269s0012.1.v1.1
MMEEKPKFKTVQEVVAANEGLPERYLQPLTGDHGYIPSIDLSLLLSSSEELSKLHSALSKWGVVQVMNHGITEVFLDKINKLTKQFFALPTEEKQKYARDIGNVQGYGSDMILSDDQVLDWIDRLYLTTYPEDQHLLKFWPEVPIEFRETLHEYTRKQHAVVEQFFKGVARSLDLEENVFLEMHGENGRMDTRFNMYPPCPSPDKVIGAKPHSYGSAFTLLLPDKDVEGLQFLKDGKWYNAPIVNDTILINVGDMMERKRKDKTVKNYVELYFDSYQQGRRPIEAALI
>Camar.0525s0001.1.v1.1
MRDMENKTQVEDPIIQESLLTCIDLANSDLHQSALMLKQACLDSGFFYVINHGISKEVMDKTFEQSKKFFALPLDEKMKVLKNEKHRGYTPLYDQIPDPENQVQGDHKEGYYIGSEVPRDDPQWDRPFYGPNPWPNPDVVPGWRETMEKYHQEALRVCKAIAKLLALSLDLDSSYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMITLLSTDGVMGLQICKDRNAKVQKWEYVPSIEGAIVVNIGDMMERWSNGIFRSTLHRVILNGQTRYSIPFFIEPNHDCIIECLPTCQSENNLPKYPPIKCSAYLTQRYEESHLKFSSYKKQT
>Camar.0551s0016.1.v1.1
MELPVVDLSRFLQSSNGESDSHLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPQGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKEAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKNAFS
>Camar.1203s0015.1.v1.1
MEIERVQDISSSSLHTDGIPLEFIRSEKEQPAITTFRGPVPAIPVVDLSEHDEESVARAVVRASEEWGIFQVVNHGIPTELIRRLQEVGRRFFELPTAEKESVAKPADAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPKNPPEYRQVNEEYALHVKKLSESLLGILSEGLGLRREALKEGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGLTLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMIVGPLPELIGDDNPPKYKPFAFKDYSYRKLNKLPLD
>Camar.1944s0002.1.v1.1
MVFERDQIPVIDLSNPDQELVVREVVKASEEWGVFQVVNHGIPTELIQRFQNVGKRFFELCEVEKEAVAKPADSREGYARRYTLDLEKRTGTVDQLFHNICPPSLVNYSYWPKNPQDYREVTEEYTRHVKMLSEKIMEWLSEGLGLRREAIKEVVGGEYLLNINYYPPCPHPDVIEGLDAHTDVSGLTLLLTNEIPGLKVFKDDQWIDVKYIPYAVIVNVSDQILRLSNGMYKSILHKTTVDKERTRMSWAALVRPTNDMVVGPFPELISQDDPPKFRPMAYKDYIYRKIRNLPL
>Camar.2464s0005.1.v1.1
MEMARNQHMPNYKWDIPIVDLSNPDENLVARAVVKASEEWGIFQVVNHGIPDELIRRLKEVGTEFFELPETEPSSIIPDNGDVLNLPVDTEKEAIAKPEDSMDVEGYITKYQTDLEEYGHSDKPQNSNLYNTPDHKWQTILKLRCRLTIPVTNLITISLKDHGLVHHMDIVYHSCKFSEFLIINFFDYEFYFTREVNEDYARHLKKLSEKIMEWLLEGLGLRSKALKECLGGETVEYLMKINYYPPCPDQDLVIGAPEHTDVNGITFLVANEALGLQAFKDNNWIDVKYTTSGIIVIVGDQFHNSEIF
>Camar.2464s0006.1.v1.1
MEWLSEGLGLQSEALEEGLGGEAVECLMNINYYPPCSDQDLVIGATDHTDVNGITLLVANEALGLQTFEENHWIDVKYTTSKIVVIVGDKFHRMSNGKYKSANNRERMDKYKTRISWPVFVESSLVHEFAPWPAEDNATKFKS
>Camar.2464s0001.1.v1.1
MKINYYPPCPDQDLVIGVPDHTDVNGITFLVANEALGLQAFKDNHWIDVKYTTYEIIVIVGDQFHRMSNGKYKSARHRATMDKEKTRISWPVFVESSLDHEFAPLPELITGEDNAPKFKPYL
>Camar.2464s0004.1.v1.1
MEMARNQHMPKYKWDVLIVDLSNRDEDLVARAVVKASEEWGIFQVVNHGIPEELIRRLKEVGTEFFELPETEKEVVAKPEDSMDVEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVSYRFWPKVPMAYMEVNEDYAKHLKKLSEKIVEWQSEGLGLRSEALQEGLGGETVEYLMKINYYPPCPDQDLVIGAPEHIDVNGITFLVANEVLGLQASKDNNWFDIKYTTSGIIVIVGDQFHRMSNGKYKSAKHRAKMDKEKTRISWPVFVEYSLDHEFAPLPELITGEDNAPKFKPYLYKDFKFRKLKKFPLD
>Camar.2464s0007.1.v1.1
MEIERNQHIPLEETILAPEEIPVVDLSNPDEDLVARAVVKASEEWGIFQVVNHGIPQELIRRLKKVGTEFYELPETEKKAVAKPEDDTDIEGYTTNFKGWADHLFHRIWPPSRVNYRFWPKNSPDYRAVNEEYASEIKKLSEKIMEWLSEGLGLRREALKEGLGGENVEYLMKLIFYPPRPDPDLNDGAPPHTDFNGMTFLIANEVYGLQAFKDNKWIDVKYDDSMILVIIADQFHRMSNGKYKSAEHRATRDTEKTRISWPVFVEPNLDQVVGPLPELLSSDSTAPKFKSYAYIDFKFHVRYGLPLV
>Camar.2709s0001.1.v1.1
MEVKRDQHTSPPTETIPIVDLSDLNEDLVSRAVVKASQEWGIFHVVNHGISMDLIRRLKEVGTQFFELPETEKKAVAKPDTSEDYEGYTRNLKYTEGEVWADNLFHRIWPPSCINYNYWPKNPPQYREVIEEYTKETKKLSERLLGVLSEGLGLRHEALKEGLGGGKTEYVLRINNFPPNPQPDANLGLPEHTDIIAIALIVTNEVPGLQVFNDDHWFDVQYIPSSITVTICDQILRLSNGKYKNVLHRVTVDKEKQRMSWPVFVDANPDVVIQPLPELITDDNPSMFEPIICKEFKYRSLFKLLID
>Camar.5624s0001.1.v1.1
MELPVVDLSRFLQSSNGESDSHLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPQGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKEAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKNAFS
MEVERVHYTSPPSETVPIVDLSDPDDELVARTLVKASEEWGAFQVVNHGIPAELMRRFQDVGRQFFELPTAEKEAVAMRPGSKSIEGYDARNPKLAKSWDDHLLHKIWPQSCIDYRFWPKNPPDYREVTEEYASHVKKLSEKIMGYLSKGLGLELETLKERLGGGEYMLRINYYPPSDSVIGAPAHTDFDALALLVSNGVPGLQVFKDSHWLDVEDINSAIVIILGDQMMRLSNGRYKSVLHRSTMDKEKTRMTWPVLIDPMPGMVVGPLPEIIGDENPPKFESLTLEEYIYRRTNMLLGDG
>Camar.0037s0038.1.v1.1
NLDKELVAAAVVKASQEWGVFQIVNHEIPEELIKNLKKVGREFFELSSEAEKEAVATPANSKDIQGYVKTSHKDVEDKVDRVDHLFHNLSPPSWIDYRYWPKNPPEYRQVNEEYAVYVKKLAEKILGWLSEGVGLDREALFNKGFGGESAIALYNLFCSSLVSNIICFKKTESYIYLTKAKRMSNGKYKNVLHRTTLNKEKTRMSWAVHLKPAYDMVIEPVMKLTSHGDEAPKFNPITYGELVYRRVNNLPLD
>Camar.0037s0037.1.v1.1
MMDKEKTRMTWQVLIDPMPGMVVGPLPELIGDENPPNLLLNLSATKISPCSSLLIITNTSRSVNSLPTEYELSELERNQNTFRGTSMDVPIIDLSNSDEELVARAVVKASEEWGIFQVVNHEIPVELIRRLKEVGTEFFELPETEKELVAKPTDSMDLEGYITKFRKDLEGRDAWIDHLFHRIWPPSRVTYRFWPKNPPEYREVNEEYARHIKKLSEKIMKWLSEGLGLRREALKEGLGGETVEYLMKINYYPQCPDQDLVVGAPAHTDVNGITFLVANEASGLQALKDNHWIDTTCTTSGIFVIIGDQFRMMSNGKYKGAPHRATMVKEKTRISWPVFVESSLDHEFGPLPELLTGEDNAPKFKPIVYKEHKFRKPKNLVLD
>Camar.0037s0040.1.v1.1
MEVETVPIIDLSGDLDDELVTHAMVKASEEWGVFQVVNHGIPAELMRRLQEVGRQFFELPAEEKEAVATLPPGSKSLQGYGAKNPKLGQSWNDHLFHKIWPQSCVDYRFWPKNPSDYR
>Camar.0269s0012.1.v1.1
MMEEKPKFKTVQEVVAANEGLPERYLQPLTGDHGYIPSIDLSLLLSSSEELSKLHSALSKWGVVQVMNHGITEVFLDKINKLTKQFFALPTEEKQKYARDIGNVQGYGSDMILSDDQVLDWIDRLYLTTYPEDQHLLKFWPEVPIEFRETLHEYTRKQHAVVEQFFKGVARSLDLEENVFLEMHGENGRMDTRFNMYPPCPSPDKVIGAKPHSYGSAFTLLLPDKDVEGLQFLKDGKWYNAPIVNDTILINVGDMMERKRKDKTVKNYVELYFDSYQQGRRPIEAALI
>Camar.0525s0001.1.v1.1
MRDMENKTQVEDPIIQESLLTCIDLANSDLHQSALMLKQACLDSGFFYVINHGISKEVMDKTFEQSKKFFALPLDEKMKVLKNEKHRGYTPLYDQIPDPENQVQGDHKEGYYIGSEVPRDDPQWDRPFYGPNPWPNPDVVPGWRETMEKYHQEALRVCKAIAKLLALSLDLDSSYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMITLLSTDGVMGLQICKDRNAKVQKWEYVPSIEGAIVVNIGDMMERWSNGIFRSTLHRVILNGQTRYSIPFFIEPNHDCIIECLPTCQSENNLPKYPPIKCSAYLTQRYEESHLKFSSYKKQT
>Camar.0551s0016.1.v1.1
MELPVVDLSRFLQSSNGESDSHLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPQGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKEAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKNAFS
>Camar.1203s0015.1.v1.1
MEIERVQDISSSSLHTDGIPLEFIRSEKEQPAITTFRGPVPAIPVVDLSEHDEESVARAVVRASEEWGIFQVVNHGIPTELIRRLQEVGRRFFELPTAEKESVAKPADAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPKNPPEYRQVNEEYALHVKKLSESLLGILSEGLGLRREALKEGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGLTLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMIVGPLPELIGDDNPPKYKPFAFKDYSYRKLNKLPLD
>Camar.1944s0002.1.v1.1
MVFERDQIPVIDLSNPDQELVVREVVKASEEWGVFQVVNHGIPTELIQRFQNVGKRFFELCEVEKEAVAKPADSREGYARRYTLDLEKRTGTVDQLFHNICPPSLVNYSYWPKNPQDYREVTEEYTRHVKMLSEKIMEWLSEGLGLRREAIKEVVGGEYLLNINYYPPCPHPDVIEGLDAHTDVSGLTLLLTNEIPGLKVFKDDQWIDVKYIPYAVIVNVSDQILRLSNGMYKSILHKTTVDKERTRMSWAALVRPTNDMVVGPFPELISQDDPPKFRPMAYKDYIYRKIRNLPL
>Camar.2464s0005.1.v1.1
MEMARNQHMPNYKWDIPIVDLSNPDENLVARAVVKASEEWGIFQVVNHGIPDELIRRLKEVGTEFFELPETEPSSIIPDNGDVLNLPVDTEKEAIAKPEDSMDVEGYITKYQTDLEEYGHSDKPQNSNLYNTPDHKWQTILKLRCRLTIPVTNLITISLKDHGLVHHMDIVYHSCKFSEFLIINFFDYEFYFTREVNEDYARHLKKLSEKIMEWLLEGLGLRSKALKECLGGETVEYLMKINYYPPCPDQDLVIGAPEHTDVNGITFLVANEALGLQAFKDNNWIDVKYTTSGIIVIVGDQFHNSEIF
>Camar.2464s0006.1.v1.1
MEWLSEGLGLQSEALEEGLGGEAVECLMNINYYPPCSDQDLVIGATDHTDVNGITLLVANEALGLQTFEENHWIDVKYTTSKIVVIVGDKFHRMSNGKYKSANNRERMDKYKTRISWPVFVESSLVHEFAPWPAEDNATKFKS
>Camar.2464s0001.1.v1.1
MKINYYPPCPDQDLVIGVPDHTDVNGITFLVANEALGLQAFKDNHWIDVKYTTYEIIVIVGDQFHRMSNGKYKSARHRATMDKEKTRISWPVFVESSLDHEFAPLPELITGEDNAPKFKPYL
>Camar.2464s0004.1.v1.1
MEMARNQHMPKYKWDVLIVDLSNRDEDLVARAVVKASEEWGIFQVVNHGIPEELIRRLKEVGTEFFELPETEKEVVAKPEDSMDVEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVSYRFWPKVPMAYMEVNEDYAKHLKKLSEKIVEWQSEGLGLRSEALQEGLGGETVEYLMKINYYPPCPDQDLVIGAPEHIDVNGITFLVANEVLGLQASKDNNWFDIKYTTSGIIVIVGDQFHRMSNGKYKSAKHRAKMDKEKTRISWPVFVEYSLDHEFAPLPELITGEDNAPKFKPYLYKDFKFRKLKKFPLD
>Camar.2464s0007.1.v1.1
MEIERNQHIPLEETILAPEEIPVVDLSNPDEDLVARAVVKASEEWGIFQVVNHGIPQELIRRLKKVGTEFYELPETEKKAVAKPEDDTDIEGYTTNFKGWADHLFHRIWPPSRVNYRFWPKNSPDYRAVNEEYASEIKKLSEKIMEWLSEGLGLRREALKEGLGGENVEYLMKLIFYPPRPDPDLNDGAPPHTDFNGMTFLIANEVYGLQAFKDNKWIDVKYDDSMILVIIADQFHRMSNGKYKSAEHRATRDTEKTRISWPVFVEPNLDQVVGPLPELLSSDSTAPKFKSYAYIDFKFHVRYGLPLV
>Camar.2709s0001.1.v1.1
MEVKRDQHTSPPTETIPIVDLSDLNEDLVSRAVVKASQEWGIFHVVNHGISMDLIRRLKEVGTQFFELPETEKKAVAKPDTSEDYEGYTRNLKYTEGEVWADNLFHRIWPPSCINYNYWPKNPPQYREVIEEYTKETKKLSERLLGVLSEGLGLRHEALKEGLGGGKTEYVLRINNFPPNPQPDANLGLPEHTDIIAIALIVTNEVPGLQVFNDDHWFDVQYIPSSITVTICDQILRLSNGKYKNVLHRVTVDKEKQRMSWPVFVDANPDVVIQPLPELITDDNPSMFEPIICKEFKYRSLFKLLID
>Camar.5624s0001.1.v1.1
MELPVVDLSRFLQSSNGESDSHLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPQGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKEAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGHFAESSLASNYPAIPAGEYVEQELSVINLKGKNAFS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University