Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Brassica oleracea
Species name
Wild cabbage (Brassica oleracea) is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Brassica_oleracea.FLS.fa
Download
>rna-XM_013758739.1
MAEPVPSKFQGTSPVDFPIVDLSNPNEDLVARAVVKASETWGMFQVVNHGIPTELMRRLKELGTEFFELPEKEKEAVARPADSTDLEGYTTDYKKDGEGRKTWADHLFHRIWPPSRINYRFWPKNSPDYREVNEEYAKEIKKLSEKIMGWLSEGLGLHRDALKEGLGGEKVEYLMKIIFYPPCPKLELLYGAPHHTDLNGITFLIADEVDGLQAFQDNKWVDVKYDDSGIVVIIADQIKRMSNGRYKSGEHRATIDTVRTRLSWPVFAEPNLDHVVGPLPELVIDDAPKFKPYVYREYKFLKMNKLPLE
>rna-XM_013771308.1
MDVPIVDLSNPNEDLVARAVVKASEEWGIFQVVNHGIPPELMRRLKEVGTEFFELPETEKEAVAKPEDSMDVEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVSYRFWPKLPMDYREVHEDYAKHLKKLSEKIMEWLSEGLGLRREALIEGLGGETVEYLMKINYYPPCHDQDLVIGAPDHTDVNGITFLVANEALGLQAFKDNHWIDVKYTTSGIIVIVGDQFHRMSNGKYMSAKHRATMDKEKTRISWPVFVESSLDHEFAPLRELITGEDNAPKFKPYVYKDFKFRKLKKLPLD
>rna-XM_013766748.1
MVKAFATTVHTTTFHYLNQTSNRPPSPSSPSTIMEVKRYQPTSPPSETIPIVDLSNPDEELVSRAVVKASQEWGVFHVVNHGISMDLIRRLKEVGTQFFELPETEKKAVAKPDDSQDFEGYTRNLKYTEGEVWADNLFHRILPQSCINYKYWPRNPPQYREVIEEYTKETKKLAERLLGFLSEGLGLRHEALKEGLGGEKSEYVLRINNFPPNPQPDATLGLPEHTDIVALALIVTNEVPGLQVFHEGHWFDVQYVPSSVTVTIGDQILRLSNGKYKNVLHRVTVDKEKQRMSWPVFVDANPDVVIRPLPELITGDNPSMFKSIICKDFKYRRLYKLPVD
>rna-XM_013780303.1
MSGGYARKYTLDLEKRRGTVDQLFHNIWPPSAVNPKNPQDYREVNAECTKHVKMLSEKIMEWLSEGLGLRREAINEVVGGEYLLNVNYYPPCPHPDVIRGLNPHTDVSGLTLLITNEIPGFQVFKDDQLIEVEYIPFTVIVNISDQILRLSSGRYKSVLHQTTVDKERIRIRPTKDTVVGPFPELIGQDDPPKFRPMLYKDYIYRKVRYLPFSDLDS
>rna-XM_013782283.1
MENHTKEELQDPTMQVSSLNCIDLANDDLHRSVVSLKQACLDCGFFYVINHGINEEFMDDVFDQSKKFFALPLEEKMKVLRNEKHRGYTPVLDEILDPENQVNGDHKEGYYIGIEVPKDDPDYDKPFYGPNPWPDSDVLPRWRETMEKYHQEALRVSKDIGRLLALALDLDANYFDTWEMLGKPIATMRLLHYEGVSDPSKGIYACGAHTDYGMMTLLATDGVMGLQICKDKNAKPQKWEYVPAIKGAYIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSESNVPKYPAIKCSTYLTQRYKETHAELSIYNQEI
>rna-XM_013743363.1
MELPVVDLSRYLQSSNSESDPQLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMRNECKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGRFAESSLASNYPAIPAGEHVEQELSVINLKGKESSS
>rna-XM_013754182.1
MEVERDQTVSSPTIPIVDLSNLDKELVARAVVKASQEWGVFQVVNHEIPKELIQSLKKVGTHFFELSSEAEKEAVATPANSKDIQGYVTNSQQDIEDKVDRVDHLFHNLPPPTSVDYRYWPKNPPEYRQVNEEYARQVKKLAEKILGWLSEGLGLDREALFNQGFGGGEKAVYTMKINYYPPCRPQANLVLGMEPHTDIHGLTLLVPNEIPGLQVFNDNQWFDVEYNPSVINILIGDPIWRMSNGKYKNVLHRTTLNTEKTRMSWVVLLKPPYDMVIEPLTKHTGDGDEAPKFKPITYGEHVERKVRDKLQKRS
>rna-XM_013751217.1
MEIERVQDISSSSLHTEAIPLEFIRSEKEQPAITTFRGPVPAIPVVDLSDPDEESVARAVVKASEEWGIFQVVNHGIPTELIKRLQEVGRTFFELPSSEKESVAKPVDAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPKNPPEYREVNEEYALHVKKLSETLLGILSEGLGLRREALREGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMIVGPLPELISDDNPPKYKPFAFKDYSYRKLNKLPLD
MAEPVPSKFQGTSPVDFPIVDLSNPNEDLVARAVVKASETWGMFQVVNHGIPTELMRRLKELGTEFFELPEKEKEAVARPADSTDLEGYTTDYKKDGEGRKTWADHLFHRIWPPSRINYRFWPKNSPDYREVNEEYAKEIKKLSEKIMGWLSEGLGLHRDALKEGLGGEKVEYLMKIIFYPPCPKLELLYGAPHHTDLNGITFLIADEVDGLQAFQDNKWVDVKYDDSGIVVIIADQIKRMSNGRYKSGEHRATIDTVRTRLSWPVFAEPNLDHVVGPLPELVIDDAPKFKPYVYREYKFLKMNKLPLE
>rna-XM_013771308.1
MDVPIVDLSNPNEDLVARAVVKASEEWGIFQVVNHGIPPELMRRLKEVGTEFFELPETEKEAVAKPEDSMDVEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVSYRFWPKLPMDYREVHEDYAKHLKKLSEKIMEWLSEGLGLRREALIEGLGGETVEYLMKINYYPPCHDQDLVIGAPDHTDVNGITFLVANEALGLQAFKDNHWIDVKYTTSGIIVIVGDQFHRMSNGKYMSAKHRATMDKEKTRISWPVFVESSLDHEFAPLRELITGEDNAPKFKPYVYKDFKFRKLKKLPLD
>rna-XM_013766748.1
MVKAFATTVHTTTFHYLNQTSNRPPSPSSPSTIMEVKRYQPTSPPSETIPIVDLSNPDEELVSRAVVKASQEWGVFHVVNHGISMDLIRRLKEVGTQFFELPETEKKAVAKPDDSQDFEGYTRNLKYTEGEVWADNLFHRILPQSCINYKYWPRNPPQYREVIEEYTKETKKLAERLLGFLSEGLGLRHEALKEGLGGEKSEYVLRINNFPPNPQPDATLGLPEHTDIVALALIVTNEVPGLQVFHEGHWFDVQYVPSSVTVTIGDQILRLSNGKYKNVLHRVTVDKEKQRMSWPVFVDANPDVVIRPLPELITGDNPSMFKSIICKDFKYRRLYKLPVD
>rna-XM_013780303.1
MSGGYARKYTLDLEKRRGTVDQLFHNIWPPSAVNPKNPQDYREVNAECTKHVKMLSEKIMEWLSEGLGLRREAINEVVGGEYLLNVNYYPPCPHPDVIRGLNPHTDVSGLTLLITNEIPGFQVFKDDQLIEVEYIPFTVIVNISDQILRLSSGRYKSVLHQTTVDKERIRIRPTKDTVVGPFPELIGQDDPPKFRPMLYKDYIYRKVRYLPFSDLDS
>rna-XM_013782283.1
MENHTKEELQDPTMQVSSLNCIDLANDDLHRSVVSLKQACLDCGFFYVINHGINEEFMDDVFDQSKKFFALPLEEKMKVLRNEKHRGYTPVLDEILDPENQVNGDHKEGYYIGIEVPKDDPDYDKPFYGPNPWPDSDVLPRWRETMEKYHQEALRVSKDIGRLLALALDLDANYFDTWEMLGKPIATMRLLHYEGVSDPSKGIYACGAHTDYGMMTLLATDGVMGLQICKDKNAKPQKWEYVPAIKGAYIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHECLVECLPTCKSESNVPKYPAIKCSTYLTQRYKETHAELSIYNQEI
>rna-XM_013743363.1
MELPVVDLSRYLQSSNSESDPQLGTDLLDSCREVSRILKETGALLVKDPRCSAEDNDRFIDMMETYFSKPDEFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMRNECKPHIPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTKDAIKLAKEQNRSLWRVSSTLFAHIASDAELKPLGRFAESSLASNYPAIPAGEHVEQELSVINLKGKESSS
>rna-XM_013754182.1
MEVERDQTVSSPTIPIVDLSNLDKELVARAVVKASQEWGVFQVVNHEIPKELIQSLKKVGTHFFELSSEAEKEAVATPANSKDIQGYVTNSQQDIEDKVDRVDHLFHNLPPPTSVDYRYWPKNPPEYRQVNEEYARQVKKLAEKILGWLSEGLGLDREALFNQGFGGGEKAVYTMKINYYPPCRPQANLVLGMEPHTDIHGLTLLVPNEIPGLQVFNDNQWFDVEYNPSVINILIGDPIWRMSNGKYKNVLHRTTLNTEKTRMSWVVLLKPPYDMVIEPLTKHTGDGDEAPKFKPITYGEHVERKVRDKLQKRS
>rna-XM_013751217.1
MEIERVQDISSSSLHTEAIPLEFIRSEKEQPAITTFRGPVPAIPVVDLSDPDEESVARAVVKASEEWGIFQVVNHGIPTELIKRLQEVGRTFFELPSSEKESVAKPVDAKDIEGYGTKLQKEVEGKKAWVDHLFHRIWPPSCVNYSFWPKNPPEYREVNEEYALHVKKLSETLLGILSEGLGLRREALREGLGGDLAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKDRTRMSWPVFLEPHREMIVGPLPELISDDNPPKYKPFAFKDYSYRKLNKLPLD
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University