Enzyme

This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.

DFR

Enzyme

Dihydroflavonol 4-reductase

Enzyme name

EC:1.1.1.219

Entry

Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor

Class

(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase

Sysname

a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]

Reaction

(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]

Product

Species information

Actinidia chinensis

Species name

Golden kiwifruit (Actinidia chinensis) is a species of eudicot in the family Actinidiaceae (Chinese gooseberry family).

Species info

Gene sequence info

Actinidia_chinensis.DFR.fa

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>transcript:PSS36490
MKDSAAAALDTVCVTGAAGFIGSWLVMRLLERGYIVRATVRDPGNMKKVKHLLDLPKAETNLTLWKADLGEEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGVLSIIRSCTKAKTVKRLVFTSSAGTVNVQEHQQPRYDENNWSDLDFIYAKKMTGWMYFVSKTLAEKAAWEAAKENNIDFISIIPTLVVGPFIMPTFPPSLITALSPITGNEPHYSIIKQGQFVHLDDLCESHIFLYENPKAEGRYICSSHDATIFDLAKMMREKWPEYNVPSEFKGFDENLPNVSFSSEKLIGMGFKFKYSLEDMFRGAIDTCREKGLLPYSNETPPNGNANGKV
>transcript:PSS16240
MEDEESRVEGLSAERGILRYCVTGATGYIGSWLVKSLLQRGYTVHTTVRDPAKTMNLLPLWGGGDRLRVFRADLREEGSFDEAVKGCSGVFHVAASMEFDVPAKEDSDSYARENFIDPAIDGTLNLLKSCSKTASVKRVVFTSSISTLTAKDRDGQWRSIVDESCQTPIDHVWKTKAGAWVYVLSKLLTEETAFKFANENGIDLVSVITTTVTGPFLTPTVPSSVRVLLSPITGDPKFSSILSAVNSRMGSIALVHIEDICSAHIFLMEHARAEGRYICSSHNYLMSELVDRLDKEYRSSNKPSCRLVEEDHGSAPSEISSKKLKDLGFNFKYGVDDIIHQTVKSCVDCGFLSPIVN
>transcript:PSS06053
MKDTTTADATPGTVCVTGAAGFIGSWLVMKLLERGYIVSATVRDPGNMKKVKHLLDLPKAKTNLTLWKADLAEEGSFDKAIEGCSGVFHVATPMDFESKDPENEVIKPTINGVLSIIRSCSKAKTVKRLVFTSSAGTVNVQEHQQPLYDENNWSDLDFIYAKKMTGWMYFVSKTLAEKAAWEAAKENNIDFISIIPTLVVGPFIMPTFPPSLITALSPITGNEPHYSIIKQGQFIHLDDLCESHIFLFENPKAEGRYICSSHDATIFDLAKMMREKWPEYNVPTEFKGFDENLPIVSFSSKKLIGMGFKFKYSLEDMFRGAIDTCREKGLLPYSNEAPPNGNANANANGKV
>transcript:PSR99660
MEGEHGGTTVCVTGASGFIGSWLVMRLLERGYYVRATVRDPANMSKVRHLLDLPNAATRLSLWKADLVDEGSFDDAVHGCAGVFHVATRTDFSSVKDPESEVIKPTVNGVLNIMRSCSKAESVKRLIYTSTTGTISRQQHPQSEYDESFWTDIDFCRAQKMAGWMYLVAKTMAEKAAWEFAKENGLDLVTIQPSLVLGPFITPLMPASIKISLALITRNETFYPLLARLCAVHLDDVCNAHIYLFEHVQAKGRYICSSHCFTIFEITKWLSQKYPEFDIPTKFEGIDESLNPIPCSSKKLVDLGFKFKYNSEECHVGGLFTEAIESCREKRLMPL
>transcript:PSR99661
MEGEHGGTTVCVTGASGFIGSWLVMRLLERGYYIRATVRDPANMSKVKHLLDLPNAGTYLSLWKADLTEEGSFDDAIHGCAGVFHVATPMEFISVMDPESEVIKPTVNGALNIMRSCSKAKTVKRVIYTSTTGTIVIQQHPQSEYDESFWTDVDFCKAQKMTGWMYFVAKTMAEKAAWEFAKENDLDLVTIQPSIVLGPFITPSRPTSIEISIALITRNEAFYPMLTQARAVHLDDLCNAHIYLLEYPQASGRYICSSHCFTIFDIAKWLSQKYPECDIPTKFEGVDESLKPIPCSSKKLVDLGFKFKYNSDECHVGVLFTEAIESCRQKGLMPL
>transcript:PSR99659
MAGEEGGTTVCVTGASGFIGSWLVMRLLERGYYVRATVRDPFNEDKVKHLLELGSASTHLSLWKADLNEEGSFDDAIHGCAGVFHVATPMSFYFVKEPENEFIKPTVDGVLNVMRSCCKAKTVKRIIYTSTTGTIGIQQQPPTHYDESVWTDVDFCRTTKMFAWMYFVAKTEAEKAAWKFAEENGLDLVTIQPSSVIGPFITTNRPPCTENALALITKNRAFYTMLAQSHAVNVDDVCNAHIYLFEHPQAAGRYICSSHSFTIYDLANSLSKKYPEYDIQTKFEGIDESLKPIPCPSKKLLDLGFRFKFNSQEYDVADLFAEAIEACREKGMMPPL

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University