Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Bonia amplexicaulis
Species name
Bonia amplexicaulis is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Bonia_amplexicaulis.HI4_OMT.fa
Download
>Bam014481.2
MALRCAVKLGIPNAIHRCGGAASLPELLAAIPLPANKQHYLLRLMRFLTASGIFVTDEAPITVGEGGTEAAAGGIYSLTPVSRLLVDDAGARGCASMSPLVLSQTTLFHFTAAVHLAEWFKDEEATAAAETPFKAAYGTELWGVMARDAEFSAVFNDGLGSDSRFLVGLLVGGECREVFEGISSLVDVGGGIGTAARAIATAFPDIKCSVLDLPQVVDDIPADGLVEFVAGDMMDFIPPADAVLLKLVLHDWSDEECVKILTRCRNAIPSQGKVIIVESVFGSSPSRVMHQAQLLLDQCMMVLTTGKEREEHEWHKMFVDAGFSHYKVKPVLGVLSIIEVYP
>Bam014480.2
MALRCAVKLGIPNAIHRCGGAASLPELLAAIPLPANKQHYLLRLMRFLTASGIFVTDEAPITVGEGGTEAAAGGIYSLTPVSRLLVDDAGARGCASMSPLVLSMTTLFYFTAAVHLAEWFKDEEATAAAETPFKAAYGTELWGVMARDAEFSAVFNDGLGSDSRFLVGLLVGGECREVFEGISSLVDVGGGIGTAARAIATAFPDIKCSVLDLPQVVDDIPADGLVEFVAGDMMDFIPPADAVLLKFVLHDWSDEECVKILTRCRNAIPSQGKVIIVESVLGSSPSRVMHQAQLLLDLGMMVLTAGKEREEHEWHKMFVDAGFSHYKVKPVLGVLSIIEVYP
>Bam003811.1
MAHVQEDEQMSTEDLLQAQTELYHHCFAFVKSLALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRIMRVLTVSGIFAAEDRDEETVYKLTRVSRLLVGHGESSHHLSPMVGVIVNPVAVTAHFSMCEWLTDDRAAAMSLFEVAHSCTRWGMIEKDAADGSVFNAGMVADSRVVMEIILRESSGIFQGVSSLVDAGGCHGAAAAAIARAFPHVKCTVLDLPHVVAGAPTDSSVQFVTGDIFEYIPPADVVLLKWILHCWQDEDCVKILRRCKEAIPARDAGGKLIIIDMVFGSGLHEIVSKETQALYDVFMMNYDGIEREEHEWRKILIEAGFSDYKITPVQGIRSIIEVYP
>Bam004134.1
MARTHDELSTESLLQGYVELYQHFFCYLKSMALRCASDLGIPSAIHRRGGAATLSDLIAETALHPAKLPHLRRLMRVLTVSGIFAASQPSPDSSCEVVYTLTPPSRLLVGDKASCDMSVLLRFHVHPTTITPFFRLEEWFRDDRAGGAAATSLFETAHGIARWDMTAKDPVYNRALNDSMVADSCFVMDVLLREGGDIFRGLSSLVDVGGGHGGAAAAIARAFPHVKCTVLDLPQVINQAPADGALQFVSGDMFESIPPADAVLLKVLRKCKEAIPARDAGGKVIIVDVAVGSRPHDNALTETQVLFDMYTMFSVGH
>Bam021531.1
MAHVQEDEQMSTEDLLQAQTELYHHCFAFVKSMALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRLMRVLTVSGIFAAEDCDGETVYKLTRVSRLLVGRGESSRHLSPMVGVLVNPRAVTTHFSMSEWLTNDRAAAMSLFEVAHGYTWWGMIEKDAAFGSVFNAAMVADSRVVMEIILRESSGVFQGVSSLVDAGGGHGAVATAIATAFPHVKCTVLDFPHVVAGAPTDSSVQFVAGDIFEYIPPADAVLLKFSFSLLTALLQVFGSRVETYVQSDFGWVLIKILISCLVLAF
>Bam021532.1
MVHVQEDEQMSTEDLLQAQTELYHHCFAFVKSMALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRLMRVLTVSGIFAAEDCDGETVYKLTRVSRLLVGHGESSRHLSAMVGVLVNPVYVTAHFSMSEWLTDDRAASMSLFEVAHGCTRWGMTDKDAADGSVFNAGMVADSRVVMEIILRESSGIFQGVSSLVDAGGCHGAAAAAIARAFPHVKCTVLDLPHVVAGAPTDSSVQFVAGDIFEYIPPADVVLLKIGRIMA
>Bam021533.2
MVHVQEDEQMSTEDLLQAQTELYHHCFAFVKSMALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRLMRVLIISSIFGAEDRGGETVYKLTRVSRLLVGRGESSRNLSPMVGAIVYPTAVTAHFSMSEWLTDDRAAAMSLFEVAHGCTRWGMIEKDAADGSVFNAAMVADSRVVMEILLRESSGIFQGVSSLVDAGGCHGAVAAAIAKALPHVKCTVLDLPHVVAGAPTDSSVQFVAGDIFEYIPPADVVLLKWILHCWQDEDCVKILRRCKEAIPARDAGGKVIIIDMVVGSGLHEIVSKEAQVLYDVFMMNYDGIEREEHEWRKILIEAGFNDYKITPVSGVRSIIEVYP
MALRCAVKLGIPNAIHRCGGAASLPELLAAIPLPANKQHYLLRLMRFLTASGIFVTDEAPITVGEGGTEAAAGGIYSLTPVSRLLVDDAGARGCASMSPLVLSQTTLFHFTAAVHLAEWFKDEEATAAAETPFKAAYGTELWGVMARDAEFSAVFNDGLGSDSRFLVGLLVGGECREVFEGISSLVDVGGGIGTAARAIATAFPDIKCSVLDLPQVVDDIPADGLVEFVAGDMMDFIPPADAVLLKLVLHDWSDEECVKILTRCRNAIPSQGKVIIVESVFGSSPSRVMHQAQLLLDQCMMVLTTGKEREEHEWHKMFVDAGFSHYKVKPVLGVLSIIEVYP
>Bam014480.2
MALRCAVKLGIPNAIHRCGGAASLPELLAAIPLPANKQHYLLRLMRFLTASGIFVTDEAPITVGEGGTEAAAGGIYSLTPVSRLLVDDAGARGCASMSPLVLSMTTLFYFTAAVHLAEWFKDEEATAAAETPFKAAYGTELWGVMARDAEFSAVFNDGLGSDSRFLVGLLVGGECREVFEGISSLVDVGGGIGTAARAIATAFPDIKCSVLDLPQVVDDIPADGLVEFVAGDMMDFIPPADAVLLKFVLHDWSDEECVKILTRCRNAIPSQGKVIIVESVLGSSPSRVMHQAQLLLDLGMMVLTAGKEREEHEWHKMFVDAGFSHYKVKPVLGVLSIIEVYP
>Bam003811.1
MAHVQEDEQMSTEDLLQAQTELYHHCFAFVKSLALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRIMRVLTVSGIFAAEDRDEETVYKLTRVSRLLVGHGESSHHLSPMVGVIVNPVAVTAHFSMCEWLTDDRAAAMSLFEVAHSCTRWGMIEKDAADGSVFNAGMVADSRVVMEIILRESSGIFQGVSSLVDAGGCHGAAAAAIARAFPHVKCTVLDLPHVVAGAPTDSSVQFVTGDIFEYIPPADVVLLKWILHCWQDEDCVKILRRCKEAIPARDAGGKLIIIDMVFGSGLHEIVSKETQALYDVFMMNYDGIEREEHEWRKILIEAGFSDYKITPVQGIRSIIEVYP
>Bam004134.1
MARTHDELSTESLLQGYVELYQHFFCYLKSMALRCASDLGIPSAIHRRGGAATLSDLIAETALHPAKLPHLRRLMRVLTVSGIFAASQPSPDSSCEVVYTLTPPSRLLVGDKASCDMSVLLRFHVHPTTITPFFRLEEWFRDDRAGGAAATSLFETAHGIARWDMTAKDPVYNRALNDSMVADSCFVMDVLLREGGDIFRGLSSLVDVGGGHGGAAAAIARAFPHVKCTVLDLPQVINQAPADGALQFVSGDMFESIPPADAVLLKVLRKCKEAIPARDAGGKVIIVDVAVGSRPHDNALTETQVLFDMYTMFSVGH
>Bam021531.1
MAHVQEDEQMSTEDLLQAQTELYHHCFAFVKSMALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRLMRVLTVSGIFAAEDCDGETVYKLTRVSRLLVGRGESSRHLSPMVGVLVNPRAVTTHFSMSEWLTNDRAAAMSLFEVAHGYTWWGMIEKDAAFGSVFNAAMVADSRVVMEIILRESSGVFQGVSSLVDAGGGHGAVATAIATAFPHVKCTVLDFPHVVAGAPTDSSVQFVAGDIFEYIPPADAVLLKFSFSLLTALLQVFGSRVETYVQSDFGWVLIKILISCLVLAF
>Bam021532.1
MVHVQEDEQMSTEDLLQAQTELYHHCFAFVKSMALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRLMRVLTVSGIFAAEDCDGETVYKLTRVSRLLVGHGESSRHLSAMVGVLVNPVYVTAHFSMSEWLTDDRAASMSLFEVAHGCTRWGMTDKDAADGSVFNAGMVADSRVVMEIILRESSGIFQGVSSLVDAGGCHGAAAAAIARAFPHVKCTVLDLPHVVAGAPTDSSVQFVAGDIFEYIPPADVVLLKIGRIMA
>Bam021533.2
MVHVQEDEQMSTEDLLQAQTELYHHCFAFVKSMALRAATDLRIPDAIHRCGGAATLSDLASGTGLHPTKLSHLRRLMRVLIISSIFGAEDRGGETVYKLTRVSRLLVGRGESSRNLSPMVGAIVYPTAVTAHFSMSEWLTDDRAAAMSLFEVAHGCTRWGMIEKDAADGSVFNAAMVADSRVVMEILLRESSGIFQGVSSLVDAGGCHGAVAAAIAKALPHVKCTVLDLPHVVAGAPTDSSVQFVAGDIFEYIPPADVVLLKWILHCWQDEDCVKILRRCKEAIPARDAGGKVIIIDMVVGSGLHEIVSKEAQVLYDVFMMNYDGIEREEHEWRKILIEAGFNDYKITPVSGVRSIIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University