Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Boehmeria nivea
Species name
Chinese silk-plant (Boehmeria nivea) is a species of eudicot in the family Urticaceae (nettle family).
Species info
Gene sequence info
Boehmeria_nivea.COMT.fa
Download
>Bni12T017163.1
MEEERRERRNEARLRILELANMISVPMSLNAAVRLGVPDAVWQGGANEPLSASEILTRVLPRGGDAENLQRILRMLASYGVFTEHLGSGDGGRGGGEERKYSLTDVGRTLATDADGLSYAPYVLQHHQEALMRAWGMVEEAVVDPTREPFARANGEAAYEYYGKRPEMNGLMQRAMSGVSVPFMRAILDGYDGFVGVRRLVDVGGSAGDCLRMILAKFPSLREGVNFDLPEVVARAPPIPGVTHVGGDMFKSIPDGDAIFMKWILTTWTDDECKSIMENCYKALPEGGKLIACEPVLPKETDDSHRTRALLEGDIFVMTIYRAKGKHRTEEEFQKLGLSAGFPNFRAIYIDYFYAVLEFQK
>Bni03T005033.1
MNRHHTPLALSKHSDQSNMGSNGGETQMTPTQVSDEEANLFAMQLASASVLPMVLKTAIELDLLEIIAKAGPGAWLSPAEIAAQLPTTNPAAPVMLDRILRLLACYSVVACQLRTLPGGAAERLYGLAPVCKFLTKNNDGVSISPLCLMNQDKVLMESWYYLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKLLETYKGFEGLNSVVDVGGGTGAVLSMIVSNYPSIKGINFDLPHVIEDAPAYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYDALPNNGKVIVAECILPVAPDASLATKGVVHIDVIMLAHNPGGKERTEKEFQALAKGAGFQGFRVVCSAFNTYPIVIVAVVVHMSGAQQQVVLGSGGEADGEEEEEERLFLWALQLGSASVLTMALRAAIELGLFDLMAAAGPAALLSPSDISSRLHLHHPAAAAAAGGGRLGRVMHLLACYSVLTCSSRDLPDGKVERLYGIGAVGKLFLNNGNGVSFAPRSLFSTENTFTKSCCHLKDAVMRNKSMVAMKEILKVYKGFQGLNSLVDVGGGTGVSINMIVSNYPHIKAINFDLPHVVADAPPYPGVEHVGGDMFSIIHDWDDEDCLRFLKNCYNALPENGKVIVVGFIVPEVAVNSLAMKTIVFADVLMLIQTPNGKERTQKEYEALANASGFQAFRVVSSALDYHVMEYHLKDALVEGGVPFNMAHGMTLSEYQQTDPQFNKVFNDGMSDYSAITMKKILRSYTGFEGHGSVVNVGGGAGANLGMVVSKYPSIKGINFDLPHVIREAPMYSGVEHIAGDMFESVPKGDAIFMKFSSN
>Bni07T010617.1
MDHPPMKRKIAPNKQCNSQARRFFPMILKAVVELGVLIVVDAGQGLNGYLWRNEGGHPGERGHGMNIVECVSKDSRFGELFKHVGGDDGSILSMIISNYPTIKGINFDLANIVEKSPRYPECKWLG
>Bni08T011792.1
KKPISFGMQRPNVPKATFNHGNSTTVPVEDRSKEEEEESYSYAMQLVLSSALSMSMHSAIELGVFDIIAKAGEGAKVSSAEIAGQLGNNTNPEAPMMLDRILRLLASHSVLSCTVVGDDESCTSNVQRLYGLAPVSKYFVTNEDGVSLGPLMALIQDKVFLNSWRNSIQQALRNTRFRVSGLGPEVFNTAMYNQTTIVIKNILKFYKGFENLKQLVDVGGGLGVTLSIITSKYPHIKAINFDLPHVIKHAPPDPGVEHWILHDWSDEHRLKLLKNCYKAIPNDGKVIVVEATLPSYPQTSSAVKSTSELDVFMMTQNPGGKERSKQEFIALATKAGFSGIKFDCFLLIALPLPKTPQKNNIHPLQIPAMASFSEHENSTTVPIGDQSRVEEEENFSYAMQLVCSSALSVSLHSAIELGVFDIIAKAGEGAEISSAEIAGQMANNTNPEAPMMLDRILRLLASHSVLSCTVVGDDETCTSNVRRLYGLAPVSNYFVTNEDGVSLGPLMALVQDKIHGKNAFEYQGSDLRFNHLFNKALYNKTTMVIKSILKSYKGFENLKQLVDWILHDWSDKHCLKLLKNCYKAIPDDGKVIVVEAVSSGKPEANSAAKTTSEIDIILMTLFQGGKERSKQEFLALATEAGFSGIKFGCFVCNYFWVMEFYK
>BniUnT019289.1
MSHLVELENQCPKHVDDHNTNTEEFEDDCERENFCYAVQLAFSSVLPMSLHAAAELGVFDIIAGAGRELSGEEIAAQLPTRNPEAGAMLDRILRLLASHSVLKCRLVGDEKLGLDFCKRVYSLSPVAKYFVTNEDGVSLKPFMALIQDKVILDSWSHLKEAILEGGLPFNRAHNVHAFEYTELDQRFNKLYNTAMYNVTTIVVKKILESYKGFEQIKNLVDVGGNLGVTLSLIVSKYPHIRATNFDLPKVIEHAPVYPGVEHVGGDMFETVPTGDAILLKSILHDWSDEKCLKILENCYKATPENGKVIVIEEILPVMPETKTAVKSTSQTDVIMMTKNPGGKERSKHEFLALATVVGFIGIKFERCGCNYWVMEFYK
MEEERRERRNEARLRILELANMISVPMSLNAAVRLGVPDAVWQGGANEPLSASEILTRVLPRGGDAENLQRILRMLASYGVFTEHLGSGDGGRGGGEERKYSLTDVGRTLATDADGLSYAPYVLQHHQEALMRAWGMVEEAVVDPTREPFARANGEAAYEYYGKRPEMNGLMQRAMSGVSVPFMRAILDGYDGFVGVRRLVDVGGSAGDCLRMILAKFPSLREGVNFDLPEVVARAPPIPGVTHVGGDMFKSIPDGDAIFMKWILTTWTDDECKSIMENCYKALPEGGKLIACEPVLPKETDDSHRTRALLEGDIFVMTIYRAKGKHRTEEEFQKLGLSAGFPNFRAIYIDYFYAVLEFQK
>Bni03T005033.1
MNRHHTPLALSKHSDQSNMGSNGGETQMTPTQVSDEEANLFAMQLASASVLPMVLKTAIELDLLEIIAKAGPGAWLSPAEIAAQLPTTNPAAPVMLDRILRLLACYSVVACQLRTLPGGAAERLYGLAPVCKFLTKNNDGVSISPLCLMNQDKVLMESWYYLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTITMKKLLETYKGFEGLNSVVDVGGGTGAVLSMIVSNYPSIKGINFDLPHVIEDAPAYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYDALPNNGKVIVAECILPVAPDASLATKGVVHIDVIMLAHNPGGKERTEKEFQALAKGAGFQGFRVVCSAFNTYPIVIVAVVVHMSGAQQQVVLGSGGEADGEEEEEERLFLWALQLGSASVLTMALRAAIELGLFDLMAAAGPAALLSPSDISSRLHLHHPAAAAAAGGGRLGRVMHLLACYSVLTCSSRDLPDGKVERLYGIGAVGKLFLNNGNGVSFAPRSLFSTENTFTKSCCHLKDAVMRNKSMVAMKEILKVYKGFQGLNSLVDVGGGTGVSINMIVSNYPHIKAINFDLPHVVADAPPYPGVEHVGGDMFSIIHDWDDEDCLRFLKNCYNALPENGKVIVVGFIVPEVAVNSLAMKTIVFADVLMLIQTPNGKERTQKEYEALANASGFQAFRVVSSALDYHVMEYHLKDALVEGGVPFNMAHGMTLSEYQQTDPQFNKVFNDGMSDYSAITMKKILRSYTGFEGHGSVVNVGGGAGANLGMVVSKYPSIKGINFDLPHVIREAPMYSGVEHIAGDMFESVPKGDAIFMKFSSN
>Bni07T010617.1
MDHPPMKRKIAPNKQCNSQARRFFPMILKAVVELGVLIVVDAGQGLNGYLWRNEGGHPGERGHGMNIVECVSKDSRFGELFKHVGGDDGSILSMIISNYPTIKGINFDLANIVEKSPRYPECKWLG
>Bni08T011792.1
KKPISFGMQRPNVPKATFNHGNSTTVPVEDRSKEEEEESYSYAMQLVLSSALSMSMHSAIELGVFDIIAKAGEGAKVSSAEIAGQLGNNTNPEAPMMLDRILRLLASHSVLSCTVVGDDESCTSNVQRLYGLAPVSKYFVTNEDGVSLGPLMALIQDKVFLNSWRNSIQQALRNTRFRVSGLGPEVFNTAMYNQTTIVIKNILKFYKGFENLKQLVDVGGGLGVTLSIITSKYPHIKAINFDLPHVIKHAPPDPGVEHWILHDWSDEHRLKLLKNCYKAIPNDGKVIVVEATLPSYPQTSSAVKSTSELDVFMMTQNPGGKERSKQEFIALATKAGFSGIKFDCFLLIALPLPKTPQKNNIHPLQIPAMASFSEHENSTTVPIGDQSRVEEEENFSYAMQLVCSSALSVSLHSAIELGVFDIIAKAGEGAEISSAEIAGQMANNTNPEAPMMLDRILRLLASHSVLSCTVVGDDETCTSNVRRLYGLAPVSNYFVTNEDGVSLGPLMALVQDKIHGKNAFEYQGSDLRFNHLFNKALYNKTTMVIKSILKSYKGFENLKQLVDWILHDWSDKHCLKLLKNCYKAIPDDGKVIVVEAVSSGKPEANSAAKTTSEIDIILMTLFQGGKERSKQEFLALATEAGFSGIKFGCFVCNYFWVMEFYK
>BniUnT019289.1
MSHLVELENQCPKHVDDHNTNTEEFEDDCERENFCYAVQLAFSSVLPMSLHAAAELGVFDIIAGAGRELSGEEIAAQLPTRNPEAGAMLDRILRLLASHSVLKCRLVGDEKLGLDFCKRVYSLSPVAKYFVTNEDGVSLKPFMALIQDKVILDSWSHLKEAILEGGLPFNRAHNVHAFEYTELDQRFNKLYNTAMYNVTTIVVKKILESYKGFEQIKNLVDVGGNLGVTLSLIVSKYPHIRATNFDLPKVIEHAPVYPGVEHVGGDMFETVPTGDAILLKSILHDWSDEKCLKILENCYKATPENGKVIVIEEILPVMPETKTAVKSTSQTDVIMMTKNPGGKERSKHEFLALATVVGFIGIKFERCGCNYWVMEFYK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University