Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Boea hygrometrica
Species name
Dorcoceras hygrometricum is a species of eudicot in the family Gesneriaceae (African violet family).
Species info
Gene sequence info
Boea_hygrometrica.ROMT.fa
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>rna-gnl|WGS:LVEL|mrna.F511_12807
MALPGEEESTHELLNAQTLVWNHIFKFMFPMSLKCAIELNIPEIIKNHGKPMTLSELTTALPINKAKSHYIHRMMRVLVHSKFFMKVAVNYENEQNEGYWLTPSSRLLLKDSALSIAPLFLLIHNPILMKPCSSISEWFTDDRVTAFETAHGLKFWEQAKEDSSLTKLFYEAMAGDSRFVSQVILKDCKKLFSGVGSLVDVGGGTGNMAKAIADAFPEMKCTVLDLPQVVSGLEGSSNLNYVGGDMFEAIPTADVVLLKWLLHDWDDEACIKVLKNCREVIPAGKEKGGKVIIIDIVLGYNNENDMVKEDQLLWDIAMMVYLNGKERSEKEWKQLFLDAGFSSYNITPILGVRSIIEVYP
>rna-gnl|WGS:LVEL|mrna.F511_12806
GNMFEAIPTAHAVLLKGVLHNWNDEDCIKILKKCKEAISGKEKGGKVIVIDMVLGCNDENNTIVKQNKVLHDMLMMVYMNGKERSEREWERLFLDAGFGGYKITPTLGLISIIQVFP
>rna-gnl|WGS:LVEL|mrna.F511_43146
MAKAIADSFPEMKCTVLDLPHVVSGLKGSSNLNYVAGDMFESIPTADVVLLKWILHDWNDEACIKILKKCREVIPGKEKGGKVIIIDIVLGHNEENNIVTEDQLYYDILMMIYLNGKERNEKEWKQLFLDAGFSSYNITPALGVRSIIEVYP
>rna-gnl|WGS:LVEL|mrna.F511_30368
MVLGCNDENNTIVKQNKVLQDMLMMVYANGKERSEREWERLFLDAGFGSYKITHTLGLISIIQVFP
>rna-gnl|WGS:LVEL|mrna.F511_27070
MKSDEVKDRKREIKISKWRLGFLYPEWQTRDREEVSQVFGSQPPDFNGLNHLKIATMILYEVMRLYPPVVALSRMVYEETKFGEVTLPAGKWPPVLKRNLLPEQLLKAHDHIWNQIFNFINSMSLKCAVQLGLFDIVQKHGKPITLPELISALPINKQKSHGVDRLMRILTYSKFFVKVSLDISNNQQKEGYWLTPASLLLCKDHPTSVAPFATAMLDPVLVEPWHHLEEWFRDDAPTPFVSAHGKAFWEFAGREPRMNQFFNEGMASDARFVAGVLTKDCKHVFEGLKSLVDVGGGTGTVARVMTDAFPGLKCTVLDLPHVVAGLQGTDKVNFVSGDMFDSVPTADAVFLKWILHDWNDEECIQILKRCKEAIPKKEDGGRVIMVDMVVVDDDEVTEYEGTETQYFFDIMMMAFLTGKERTEKQWKKLFLAAGFTTYKITPCVGVRSLIEIFP
>rna-gnl|WGS:LVEL|mrna.F511_17569
MHALNLAVPNGKESIDAELLDAHAHVWNHIYKFMIPMFLKCAVQLSIPDIIKNHGKPMSLSELTGVLHISKAKSHCLQRLMSLLVDSKFFVQVEEGYWLTPSSNLLLKDSSYVTPLLLLVLDPIMMKSFESVSEWFADDHATAFETAHGVKLWEKAETDSRLSELMNNEGMERHGRFESCVVLKDCENLFFGVESLVDVGGAQGAIAKAVADAFPQMKCIVLDLPHVVAGLEGSANLTYVGGNMFEAIPTAHAVLIKVYT
>rna-gnl|WGS:LVEL|mrna.F511_17570
MALPAGEGEYTNELVNAHALVWNHIFSYMLPMSLKCAVELNIPDIIKGHGKPMTLSELTSALPINKAKSHYVHRIMRVLVHSKFFKKVDVEDGSERNEGYCLTPASTLLLKDSPLCVAHFLPLALDPYLMKSCSSISEWFADDCGTAFETMHGLKFWEKAKEDSSLNEVFNEAMASDARFMSQVILKDCKKLFAGVESLVDVAGGTGTMAKAIADAFPEMKCTVLDLPHVVSGLKGSSNLNYVAGDMFESIPTADVVLLKSILHNWDDEACIKILKKCREVIPAKENGGKLIVIDMVLGYNEENNIVTEDQLYSDIVMMVYLNGKERNEKEWKQLFLDAGFSSYNITPALGVRSIIEVYP
>rna-gnl|WGS:LVEL|mrna.F511_44961
MHALNLAVPNGKESIDAELLDAHAHVWNHIYKFMIPMFLKCAVQLSIPDIIKNHGKPMSLSELTGVLHISKAKSHCLQRLMSLLVDSKFFVQVEEGYWLTPSSNLLLKDSSYVTPLLLLVLDPIMMKSFESVSEWFADDHATAFETAHGVKLWEKAKTDSRLSELMFNEGMERHGRFESCVVLKDCENLFFGVESLVDVGGAKGTIAKAVADAFPQMKCIVLDLPHVVAGLEGSANLTYVGGNMFEAIPTAHAVLIKVYT
MALPGEEESTHELLNAQTLVWNHIFKFMFPMSLKCAIELNIPEIIKNHGKPMTLSELTTALPINKAKSHYIHRMMRVLVHSKFFMKVAVNYENEQNEGYWLTPSSRLLLKDSALSIAPLFLLIHNPILMKPCSSISEWFTDDRVTAFETAHGLKFWEQAKEDSSLTKLFYEAMAGDSRFVSQVILKDCKKLFSGVGSLVDVGGGTGNMAKAIADAFPEMKCTVLDLPQVVSGLEGSSNLNYVGGDMFEAIPTADVVLLKWLLHDWDDEACIKVLKNCREVIPAGKEKGGKVIIIDIVLGYNNENDMVKEDQLLWDIAMMVYLNGKERSEKEWKQLFLDAGFSSYNITPILGVRSIIEVYP
>rna-gnl|WGS:LVEL|mrna.F511_12806
GNMFEAIPTAHAVLLKGVLHNWNDEDCIKILKKCKEAISGKEKGGKVIVIDMVLGCNDENNTIVKQNKVLHDMLMMVYMNGKERSEREWERLFLDAGFGGYKITPTLGLISIIQVFP
>rna-gnl|WGS:LVEL|mrna.F511_43146
MAKAIADSFPEMKCTVLDLPHVVSGLKGSSNLNYVAGDMFESIPTADVVLLKWILHDWNDEACIKILKKCREVIPGKEKGGKVIIIDIVLGHNEENNIVTEDQLYYDILMMIYLNGKERNEKEWKQLFLDAGFSSYNITPALGVRSIIEVYP
>rna-gnl|WGS:LVEL|mrna.F511_30368
MVLGCNDENNTIVKQNKVLQDMLMMVYANGKERSEREWERLFLDAGFGSYKITHTLGLISIIQVFP
>rna-gnl|WGS:LVEL|mrna.F511_27070
MKSDEVKDRKREIKISKWRLGFLYPEWQTRDREEVSQVFGSQPPDFNGLNHLKIATMILYEVMRLYPPVVALSRMVYEETKFGEVTLPAGKWPPVLKRNLLPEQLLKAHDHIWNQIFNFINSMSLKCAVQLGLFDIVQKHGKPITLPELISALPINKQKSHGVDRLMRILTYSKFFVKVSLDISNNQQKEGYWLTPASLLLCKDHPTSVAPFATAMLDPVLVEPWHHLEEWFRDDAPTPFVSAHGKAFWEFAGREPRMNQFFNEGMASDARFVAGVLTKDCKHVFEGLKSLVDVGGGTGTVARVMTDAFPGLKCTVLDLPHVVAGLQGTDKVNFVSGDMFDSVPTADAVFLKWILHDWNDEECIQILKRCKEAIPKKEDGGRVIMVDMVVVDDDEVTEYEGTETQYFFDIMMMAFLTGKERTEKQWKKLFLAAGFTTYKITPCVGVRSLIEIFP
>rna-gnl|WGS:LVEL|mrna.F511_17569
MHALNLAVPNGKESIDAELLDAHAHVWNHIYKFMIPMFLKCAVQLSIPDIIKNHGKPMSLSELTGVLHISKAKSHCLQRLMSLLVDSKFFVQVEEGYWLTPSSNLLLKDSSYVTPLLLLVLDPIMMKSFESVSEWFADDHATAFETAHGVKLWEKAETDSRLSELMNNEGMERHGRFESCVVLKDCENLFFGVESLVDVGGAQGAIAKAVADAFPQMKCIVLDLPHVVAGLEGSANLTYVGGNMFEAIPTAHAVLIKVYT
>rna-gnl|WGS:LVEL|mrna.F511_17570
MALPAGEGEYTNELVNAHALVWNHIFSYMLPMSLKCAVELNIPDIIKGHGKPMTLSELTSALPINKAKSHYVHRIMRVLVHSKFFKKVDVEDGSERNEGYCLTPASTLLLKDSPLCVAHFLPLALDPYLMKSCSSISEWFADDCGTAFETMHGLKFWEKAKEDSSLNEVFNEAMASDARFMSQVILKDCKKLFAGVESLVDVAGGTGTMAKAIADAFPEMKCTVLDLPHVVSGLKGSSNLNYVAGDMFESIPTADVVLLKSILHNWDDEACIKILKKCREVIPAKENGGKLIVIDMVLGYNEENNIVTEDQLYSDIVMMVYLNGKERNEKEWKQLFLDAGFSSYNITPALGVRSIIEVYP
>rna-gnl|WGS:LVEL|mrna.F511_44961
MHALNLAVPNGKESIDAELLDAHAHVWNHIYKFMIPMFLKCAVQLSIPDIIKNHGKPMSLSELTGVLHISKAKSHCLQRLMSLLVDSKFFVQVEEGYWLTPSSNLLLKDSSYVTPLLLLVLDPIMMKSFESVSEWFADDHATAFETAHGVKLWEKAKTDSRLSELMFNEGMERHGRFESCVVLKDCENLFFGVESLVDVGGAKGTIAKAVADAFPQMKCIVLDLPHVVAGLEGSANLTYVGGNMFEAIPTAHAVLIKVYT
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University