Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Betula platyphylla
Species name
Asian white birch (Betula platyphylla) is a species of eudicot in the family Betulaceae (birch family).
Species info
Gene sequence info
Betula_platyphylla.HI4_OMT.fa
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>BPChr05G22154.v1.1.679
MDLAQSQGVSVSEFFQAQSHLYKLIFSFVDSMSVHCAIKLGIPDVIHNHGQPLSLPQLVSKLHIHPNKTSCVHRLMRLLVQSGFFTKTKVHENENENEEEVYGLTPSSRLVLKDNVTSLSPFVLAMVDPVLVSPWHVLGDWLRGSTELTPFEKAHGMGLWNYCNQNPGYNCIFNEAMASDSQLMHFVVQDYKPIFEGLGSLLDVGGGTGTVARIISEAFPHIKCTVFDLPHVVANLPKTTNLEYVGGDMFHSIPSADALLIKWILHDWSDEECVKILKRCKEAITSDGKEGKLIIIDVVINEEKDEDDITKAKFLSDTLMMVLTTGKERDEKEWEKLYLEAGFSSYKIVASFGVKSVIEVYP
>BPChr05G22185.v1.1.679
MSLKCAVQLGIPDIIHNHGEPITLHDLVSALQIHPAKARFMHRLMRLLAHGGFFATTRVNKKQDEEEEAYDLTPSSRILLKENVTSLSPFVLAMLDPALVTPWQFLGHWFREDKVTAFESAHGMGFWEYGNQNPEFNHLFNEAMAGDSGMVNLVVRDCRPVSEGLDTLIDVGGGTGTCARIISEAFPHLKCTVFDLPHISTTWTNFTFFPFFFFFSNSTSNLKLNYVGGDMFQSIPLADAILLMMWLALFSKSLQMVSEPPSNAGPCAVAAVGQLAVGTKSAESTDYGIEQTKSAEHGIKRSHCADQDLPYSADHNLLYAEIKASRCNDQSTALGKIHLNHSSLAHQLITHGNTFHTQVNQHWVDCNTLSTQRKHRQQGDQAF
>BPChr05G22089.v1.1.679
MSLKSAVQLGIPDIIHNHGEPITVHDLVSALQIHPAKARFVHQLMRLLAHGGFFATTRVNKKQDEEEEAGLLSIESVDSLIFLSAYVIMDDLYGYIDIGAGTESLEDDPVAYYLGYYD
>BPChr07G18816.v1.1.679
MDQSHGQGASELFQAQSHLYKHVFSYIGSMSLKCAVQLGIPDIIHNHGKPITLHQLVSALQIHPAKARFVHRLMRLLAHCGFFAITRVNQKQEDDEEEEAYDLTPSSRILLKENVTSLSPFVVAMLDPALVTPWQFLGHWFREDKVRAFESSHGMGFWEYGNQNPEFNHLFNEAMASDSGMMNLVVRDCRPVFEGLDTLIDVGGGTGTCARIISEAFPHLKCTVFDLPHVVANSTDNLKLNYVGGDMFQSIPPAEAILLKLVLHSISDEDCVKVLKKCREAIVNKEKEGKVVIIDIVINDIKDEHEITEAKLFFDMLMMVLVTGRERYEKEWEKLFLEAGFSHYKITPIFGLRCLNLPHTTVIGFENNDKIAWVERIMQIDKRDVGVALSVISSNTSAATFLASVSLTLCSLIGAWIASSSKDFLQISKELCPCKLSDKHPDSNIPVQNVQVAVIRGGDFWSLGLRALYFALDLLLWFFGPIPMFVSSIVMVMVLHYLDTNTTLLHQHRSPGNKMVKSVAD
>BPChr07G18857.v1.1.679
MDLVQSQGVNVSELFQVQSHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPLTLPQLVSKLHIHPNKTSCVHRLMRLLVQSGFFAKTKVHENENENEEEEAYGLTPSSRLVLKDNVTSLSPFVLAMLDPVMVSPWHVLGDWLRGSTELTPFEKTHGMGLWNYCNQNPGYNCIFNEAMASDSQLMGFVVEDYKPIFEGLSSLVDVGGGTGTVARIILEAFPHIKCTVFDLPHVVANLPESSNLKYVGGDMFHSIPSADALLIKWILHDWSDEECVKILKRCKEAITSEGKEGKVIIIDVVINEDKDEDDVTKTKLLFDTFMMVLVTGKERNKKEWEKLFLEAGFSYYKIVASYGMKSVIEVYP
>BPChr07G18889.v1.1.679
MSFTTTRSSERYRSMDLVESQGVSVSHELFQVQTHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPLTLPQLVSELHIHPNKTSCVHRLMRLLVQSGFFAKTKVHENENEEEEAYGLTPSSRLVLKDNVTSLSPFVVAMLDPVLVSPWHVFGDWLRGSTELTTFEKTHGMDIWNYCKQNPGYGGIFNEAMASDSQLMRFTVEDYKPIFEGLSSLVDVGGGTGTVARIISEAFPHIECTVFDLPHVVANQPESTNLKYVGGDMFHSLPSADALLIKWILHDWSDEECVNILKRCKEAITSEGKKGKVIIIDMVINEEKDEDDVTKTKLFFDMLMMVMATGKERNKKEWEKLFLDAGFSHYKIVASYGMKSVIEVYP
>BPChr07G18937.v1.1.679
MDLAQSQGVSVSELFQAQSHLYKHIFSFIDSMSVNCAIKLGIPDVIHNHGQALSLPQLVSKLHIHPNKTSFVHRLMRLLVQSGFFAKTKVHENKNENEEEEAYGLTPSSRLVLKDNVTSLSPFVLAMLDPVMVSPWHVLGDWLRGSTELTPFEKAHGMNIWNYYNQNPEYSCIMNEAMASDSQLMGFVVEDYKPIFEGLGSLVDVGGGTGTVARIVSEAFPHIKCTVFDLPHVVANLPETTNLKYVGGDMFYSIPYADALLVKWILHDWSDEECVKILKRCKEAITSEGKKGKVIIIDVVINEENDEDDVTKTKLFFDTLMMVVVAGKERDNKEWEKLFLEAGFSSYKIVASYGMKSVIEVYP
>BPChr07G18865.v1.1.679
MDPVESQGVSVSHELFQVQSHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPITLPQLVSKLHIHPNKTSFVHRLMRLLVQSGFFDKTKVHENEEEEEAYGLTPSSRLVLKDNVTNLSPFVLAVLDPILVSPWHVLGDWLRGSTELTPFEKAHGMGVWTYFNQNPGYNCIFNEAMASDSQLMRFVVEDYKPIFEGLGSLVDVGGGTGTVARIISEAFPHIKCTVFDLPHVVANLPETSNLKYVGGDMFHSIPSADAVLIKWILHDWSDKECVKILKRCKESVTSEGKEGKVIIIDVVINEEKDEDDVTKTKFFFDTLMMVLATGKERDKKEWEKLFLEAGFSSYKIVASYGIKSVIEVYP
>BPChr07G18841.v1.1.679
MGDGNNIHFWFDHWHPAGRLFGKFGFRVVFDARTLSEVDQLKCVISKDGKYSISCTWNAIQEKHNLVNCMRIWRNSMEKCHVLDPPTICEDVLDWGLSYKVRVLREMADGREEMQLHFSPNMKSIMISNSNGFIDLMKIKVAARHFSYRTFFHTRFLVALCLHSILSTFHSFAFSIFGTIRLRFRFGYDDSMSVNCAIQLGIPDIIHNHGQPLTLQQLVSKLHIHPNKTSFVHRLMRLLVQSGFFAKTKVHENEEEEAYGLTPSSRLVLKDNVTSLSPFVLAMLDPVMVSPWHVLGEWLRGSELTPFEKVHGMGLWNYCNQNPGYNCIFNEAMASDSQLMGFMVDDYKPIFEGLGSLVDVGGGNGTVARIISEAFPHIKCTVFDLPHVVADLPESSNLKYVGGDMFHSIPSADACTLHDWSDEECLNILKRCKEAITSEGKEGKVIIIDAVINEEKDEDDFTKTKLIFDTLMMVLVVEKERDQKEWEKLFLEAGFSSYKIVASYGMKSVIEVYP
>BPChr07G18907.v1.1.679
MDLVESQGVSVSHELFQVQSHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPITLPQLVSKLHIHPNKTSFVHRLMRLLVQSGFFDKTKVHENEEEEEEAYGLTPSSRLVLKDNVTNLSPFVLAVLDPILVSPWHVLGDWLRGSTELTPFEKAHGMGVWTYFNQNPGYNCIFNEAMASDSQLMRFVVEDYKPIFEGLGSLVDVGGGTGTVARIISEAFPHIKCTVFDLPHVVANLPETTNLKFVGGDMFHSIPSADALLIKWILHDWSDEECVKILKRCKEAITSEGKEGKVIIIDMVINEEKDEDDVIKTKLLFDMLMMVFATGKERDKKEWEKLFMEAGFSHYKIVASYGVKSIIEIYP
MDLAQSQGVSVSEFFQAQSHLYKLIFSFVDSMSVHCAIKLGIPDVIHNHGQPLSLPQLVSKLHIHPNKTSCVHRLMRLLVQSGFFTKTKVHENENENEEEVYGLTPSSRLVLKDNVTSLSPFVLAMVDPVLVSPWHVLGDWLRGSTELTPFEKAHGMGLWNYCNQNPGYNCIFNEAMASDSQLMHFVVQDYKPIFEGLGSLLDVGGGTGTVARIISEAFPHIKCTVFDLPHVVANLPKTTNLEYVGGDMFHSIPSADALLIKWILHDWSDEECVKILKRCKEAITSDGKEGKLIIIDVVINEEKDEDDITKAKFLSDTLMMVLTTGKERDEKEWEKLYLEAGFSSYKIVASFGVKSVIEVYP
>BPChr05G22185.v1.1.679
MSLKCAVQLGIPDIIHNHGEPITLHDLVSALQIHPAKARFMHRLMRLLAHGGFFATTRVNKKQDEEEEAYDLTPSSRILLKENVTSLSPFVLAMLDPALVTPWQFLGHWFREDKVTAFESAHGMGFWEYGNQNPEFNHLFNEAMAGDSGMVNLVVRDCRPVSEGLDTLIDVGGGTGTCARIISEAFPHLKCTVFDLPHISTTWTNFTFFPFFFFFSNSTSNLKLNYVGGDMFQSIPLADAILLMMWLALFSKSLQMVSEPPSNAGPCAVAAVGQLAVGTKSAESTDYGIEQTKSAEHGIKRSHCADQDLPYSADHNLLYAEIKASRCNDQSTALGKIHLNHSSLAHQLITHGNTFHTQVNQHWVDCNTLSTQRKHRQQGDQAF
>BPChr05G22089.v1.1.679
MSLKSAVQLGIPDIIHNHGEPITVHDLVSALQIHPAKARFVHQLMRLLAHGGFFATTRVNKKQDEEEEAGLLSIESVDSLIFLSAYVIMDDLYGYIDIGAGTESLEDDPVAYYLGYYD
>BPChr07G18816.v1.1.679
MDQSHGQGASELFQAQSHLYKHVFSYIGSMSLKCAVQLGIPDIIHNHGKPITLHQLVSALQIHPAKARFVHRLMRLLAHCGFFAITRVNQKQEDDEEEEAYDLTPSSRILLKENVTSLSPFVVAMLDPALVTPWQFLGHWFREDKVRAFESSHGMGFWEYGNQNPEFNHLFNEAMASDSGMMNLVVRDCRPVFEGLDTLIDVGGGTGTCARIISEAFPHLKCTVFDLPHVVANSTDNLKLNYVGGDMFQSIPPAEAILLKLVLHSISDEDCVKVLKKCREAIVNKEKEGKVVIIDIVINDIKDEHEITEAKLFFDMLMMVLVTGRERYEKEWEKLFLEAGFSHYKITPIFGLRCLNLPHTTVIGFENNDKIAWVERIMQIDKRDVGVALSVISSNTSAATFLASVSLTLCSLIGAWIASSSKDFLQISKELCPCKLSDKHPDSNIPVQNVQVAVIRGGDFWSLGLRALYFALDLLLWFFGPIPMFVSSIVMVMVLHYLDTNTTLLHQHRSPGNKMVKSVAD
>BPChr07G18857.v1.1.679
MDLVQSQGVNVSELFQVQSHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPLTLPQLVSKLHIHPNKTSCVHRLMRLLVQSGFFAKTKVHENENENEEEEAYGLTPSSRLVLKDNVTSLSPFVLAMLDPVMVSPWHVLGDWLRGSTELTPFEKTHGMGLWNYCNQNPGYNCIFNEAMASDSQLMGFVVEDYKPIFEGLSSLVDVGGGTGTVARIILEAFPHIKCTVFDLPHVVANLPESSNLKYVGGDMFHSIPSADALLIKWILHDWSDEECVKILKRCKEAITSEGKEGKVIIIDVVINEDKDEDDVTKTKLLFDTFMMVLVTGKERNKKEWEKLFLEAGFSYYKIVASYGMKSVIEVYP
>BPChr07G18889.v1.1.679
MSFTTTRSSERYRSMDLVESQGVSVSHELFQVQTHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPLTLPQLVSELHIHPNKTSCVHRLMRLLVQSGFFAKTKVHENENEEEEAYGLTPSSRLVLKDNVTSLSPFVVAMLDPVLVSPWHVFGDWLRGSTELTTFEKTHGMDIWNYCKQNPGYGGIFNEAMASDSQLMRFTVEDYKPIFEGLSSLVDVGGGTGTVARIISEAFPHIECTVFDLPHVVANQPESTNLKYVGGDMFHSLPSADALLIKWILHDWSDEECVNILKRCKEAITSEGKKGKVIIIDMVINEEKDEDDVTKTKLFFDMLMMVMATGKERNKKEWEKLFLDAGFSHYKIVASYGMKSVIEVYP
>BPChr07G18937.v1.1.679
MDLAQSQGVSVSELFQAQSHLYKHIFSFIDSMSVNCAIKLGIPDVIHNHGQALSLPQLVSKLHIHPNKTSFVHRLMRLLVQSGFFAKTKVHENKNENEEEEAYGLTPSSRLVLKDNVTSLSPFVLAMLDPVMVSPWHVLGDWLRGSTELTPFEKAHGMNIWNYYNQNPEYSCIMNEAMASDSQLMGFVVEDYKPIFEGLGSLVDVGGGTGTVARIVSEAFPHIKCTVFDLPHVVANLPETTNLKYVGGDMFYSIPYADALLVKWILHDWSDEECVKILKRCKEAITSEGKKGKVIIIDVVINEENDEDDVTKTKLFFDTLMMVVVAGKERDNKEWEKLFLEAGFSSYKIVASYGMKSVIEVYP
>BPChr07G18865.v1.1.679
MDPVESQGVSVSHELFQVQSHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPITLPQLVSKLHIHPNKTSFVHRLMRLLVQSGFFDKTKVHENEEEEEAYGLTPSSRLVLKDNVTNLSPFVLAVLDPILVSPWHVLGDWLRGSTELTPFEKAHGMGVWTYFNQNPGYNCIFNEAMASDSQLMRFVVEDYKPIFEGLGSLVDVGGGTGTVARIISEAFPHIKCTVFDLPHVVANLPETSNLKYVGGDMFHSIPSADAVLIKWILHDWSDKECVKILKRCKESVTSEGKEGKVIIIDVVINEEKDEDDVTKTKFFFDTLMMVLATGKERDKKEWEKLFLEAGFSSYKIVASYGIKSVIEVYP
>BPChr07G18841.v1.1.679
MGDGNNIHFWFDHWHPAGRLFGKFGFRVVFDARTLSEVDQLKCVISKDGKYSISCTWNAIQEKHNLVNCMRIWRNSMEKCHVLDPPTICEDVLDWGLSYKVRVLREMADGREEMQLHFSPNMKSIMISNSNGFIDLMKIKVAARHFSYRTFFHTRFLVALCLHSILSTFHSFAFSIFGTIRLRFRFGYDDSMSVNCAIQLGIPDIIHNHGQPLTLQQLVSKLHIHPNKTSFVHRLMRLLVQSGFFAKTKVHENEEEEAYGLTPSSRLVLKDNVTSLSPFVLAMLDPVMVSPWHVLGEWLRGSELTPFEKVHGMGLWNYCNQNPGYNCIFNEAMASDSQLMGFMVDDYKPIFEGLGSLVDVGGGNGTVARIISEAFPHIKCTVFDLPHVVADLPESSNLKYVGGDMFHSIPSADACTLHDWSDEECLNILKRCKEAITSEGKEGKVIIIDAVINEEKDEDDFTKTKLIFDTLMMVLVVEKERDQKEWEKLFLEAGFSSYKIVASYGMKSVIEVYP
>BPChr07G18907.v1.1.679
MDLVESQGVSVSHELFQVQSHLYKHIFSYIDSMSLNTAIQLGIPDVIHNHGQPITLPQLVSKLHIHPNKTSFVHRLMRLLVQSGFFDKTKVHENEEEEEEAYGLTPSSRLVLKDNVTNLSPFVLAVLDPILVSPWHVLGDWLRGSTELTPFEKAHGMGVWTYFNQNPGYNCIFNEAMASDSQLMRFVVEDYKPIFEGLGSLVDVGGGTGTVARIISEAFPHIKCTVFDLPHVVANLPETTNLKFVGGDMFHSIPSADALLIKWILHDWSDEECVKILKRCKEAITSEGKEGKVIIIDMVINEEKDEDDVIKTKLLFDMLMMVFATGKERDKKEWEKLFMEAGFSHYKIVASYGVKSIIEIYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University