Enzyme
A cytochrome P-450 (heme thiolate) protein found in plants. The reaction involves the migration of the 2-phenyl group of the flavanone to the 3-position of the isoflavanone. The 2-hydroxyl group is derived from the oxygen molecule. EC 4.2.1.105, 2-hydroxyisoflavanone dehydratase, acts on the products with loss of water and formation of genistein and daidzein, respectively.
IFS2
Enzyme
2-hydroxyisoflavanone synthase
Enzyme name
EC:1.14.14.87
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Class
liquiritigenin, [reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating, aryl migration)
Sysname
(1) liquiritigenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',7-trihydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07777];(2) (2S)-naringenin + O2 + [reduced NADPH---hemoprotein reductase] = 2,4',5,7-tetrahydroxyisoflavanone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07778]
Reaction
2,4',7-trihydroxyisoflavanone [CPD:C15567];H2O [CPD:C00001];[oxidized NADPH---hemoprotein reductase] [CPD:C03161];2,4',5,7-tetrahydroxyisoflavanone [CPD:C12631]
Product
Species information
Betula platyphylla
Species name
Asian white birch (Betula platyphylla) is a species of eudicot in the family Betulaceae (birch family).
Species info
Gene sequence info
Betula_platyphylla.IFS2.fa
Download
>BPChr02G32253.v1.1.679
LFVAGTDTTSATLEWAMAELLHNTEALSKAKTELEQVIGKGNPMEESDIARLPYLQAVVKETLRLHVPFLLPRKAEADVEINGYIIPKDAQVLVNAWAIGRDPTLWENANSFMPERFL
>BPChr02G32255.v1.1.679
LFVAGTDTTSATLEWAMAELLHNPEILSKVKTELEQVIGKG
>BPChr02G32298.v1.1.679
GTDTTSATLEWAMAELLHNPEALSKARRELEKKIGKGNPVEESDIARLPYLQAIVKETFRLHPAVP
>BPChr08G15423.v1.1.679
MVVLELLVPTLLLALLAAVSTILFSTFQSKLPPSPIALPIIGHFHLLGPLIHQSLYRLSLRYGPLFSLRLGSVPCIVASTPDYAKEFLRNNELSFTSRKHSIAIARLTYNSSFAFAPYGPYWKFMKKLTTNELLGSRTVNAFLSHREKEYRRLLRFLAGKSEGGEAVNVTEELLKLTNNIISQMMLGRAEEARAVGFGKRIEQIHRRFDALVEKVITERQELRRRKENGEHHQLKDFIDILLDFSEDENSETKLTRANIKALIVDFFAAGTDTTAIATEWALAELINHPMMLEKAREEINNVVRNSRLVQESDVPNLPYIQAIIKETLRLHPPLPMVARKCIKDRKIGNYVIPANTLLFVNVWAIGRDPNYWKNPLEFEPERFLQSTKKDSIGLIDVRGQHFQLLPFGSGRRICPGINLAMQELPTLLAAMIQCFDFKLVGPQGEIMDGQNVVVDMEERPGLTAPRAHDLVCVPIARFRPLDILDS
>BPChr10G04396.v1.1.679
MVVLELLVPTLLLALLAAVSTILFSTFQSKLPPSPIALPIIGHFHLLGPLIHQSLYRLSLRYGPLFSLRLGSVPCIVASTPDYAKEFLRNSELSFTSRKHSIAIARLTYNSSFAFAPYGPYWKFMKKLTTNELLGSRTVNAFLSHREKEYRRLLRFLAGKSEGGIWKRIEQIHRRFECFGGEAGTDTTAIATEWALAELINHPMMLEKLVRRSIMLLEIVD
>BPChr10G04362.v1.1.679
MVARKCIKDRKIGNYVIPANTLLFVNVWAIGRDPNYWKNPLEFEPERFLQSTKKDSIGLIDVRGQHFQLLPFGSGRRICPGINWPCKSSNTARSYDPML
>BPChr10G04337.v1.1.679
MALELLVLTILVALLVSTILFSTFRSKLPPSPIALPVIGHFHLLGPLIHQSFHRLALRYGPLFSLRLGSVSCIVASTSDYAREFLKNNELSFISRQHNIAITRLTYNSSLAFAPYGPYWKFIKKLSTNELLGSRTVNMFLSLRDKEYRRFLRFLANKSEAGEAVNVTEELLKLTNNIISQMMLGRAEEARAVVREVTQIFGEFNVSDFIWFCKHLDLQGFGKRIEDISRRFDTLVEKVITERQELRKNKENGKHHHQVKDFIDILLDFSEDENLEIKLTRVHIKALIMDFFTAGTDTTAIATEWALAELINHPIVLEKARKEINNVVNNSRLVQESDVPNLPYIQAIIKETFRLHPPVPMVTRKCIEDCKIGNYVIPANTLLFVNVWAIGRDPNCWKNSLEFEPERFLQLTKKDSMGIIDVRGQHFQLLPFGSGRRICPGINLAMQELPALLAAMIQCFDFKLVGPRGEIIDDQNVVVDMDERPGLTAPRAHDLVCVPIARLSPLDILDV
>BPChr13G32248.v1.1.679
LFIAGTETSAVAMQWAIAELINHPCVLKKVREEIERVVGKSRLVEESDIPNLPYLQAVVKETLRLYPPGPVTTRECRQKCKINGFDIP*KTAVAINLYAIMRDKDSVDDPNKFRPERFLVSLLKDQEKLEQLYQNFNFVPFGAGRRGCPGTTLAFSLMNTAVASLVQCFDWKVDGDGDGGQLV
>BPChr13G07704.v1.1.679
MDILLRVCRDGKAEAKMMTRNHLKAFLVDIFVGGTGTLAEAIIWVMAELINRPNVFHKLREEIRSVVGSTRLVQESDFASLPYLQAVVKEGLRLYPPFPVTTRDCRQSCKIGGFEIPKKTVVLINLYAIMRDPEFWDNPNEFLPERFLVSDEQVQHKHVAETFLSFGAGRRSCPGSKLGLIMMQNAVAAMVQCFDWKFHGDDGHNGNKINMEVGKGVFLHLAQPLTCLPVVHFNPFASSI
>BPChr14G12891.v1.1.679
MTNLDYLTYGSADFVFATYGPYWKFMKKLCMTQLLSGRTLEQNISVRGEEIKRFLQLLHDKAKANVAVDVGAELMRLTYNIISRMALRGKCSEKEDEAEEVGELVKEMCELAGKANVSEMIWFCKNLDLQGFGKRLEDVRGRYDSMMERIMKEHEEARRKKKKEMVDGGDGKKNNMFGGGTDTSAVTMEWAISELINHPHVMAKARQEINMVIGKKRIVEESDIANLPYIQAIVKETLRLHPTGPIIVRECTEDCIVAGYEIPAKTRLFVNVWAIGRDPNHWENPLEFLPDRFLEKEGSGKRQLDVRGQHFQLLPFGSGRRSCPGATLALQVVQTTLASMVQCFEWKVGDGDDLFTVDMEEGLGVSLPRAHPLVCVPVARFSPFPSIN
>BPChr14G32310.v1.1.679
LFFASVDTTSAAILWAMTELINHPEVFKKLRVEIESVVGSSRLVKESDVPNLPYLQAVIKEALRIHPPGALLRRQCQQDCNLGGFDIKEGTKILVNIYAIMRDPKSWDEPDKFVPERFLNNNFSDQAFEQMGTKGQDFRFLPFGSGRRACMGEPHASIVLHATIGALIQCFDWELPKDGEKVDVQVVKGYAGAVAHTLVC
>BPChr14G32277.v1.1.679
WALAELINHPNVMERARQEIDSIVGNSRLVEESDIMNLPYLQAILKETLRIHPTSPIILRESSEHCTIGGYEIPVKIGLFVNVWAIRRDPKHWENPLEF
>BPChr14G12836.v1.1.679
MADLQGYIILFLVSLVSTILVRAIFHRTKTKGRLPPSPMALPIIGHLHHLTPLPHQALHKLSNQYGPLIHLFLGSVPCVVASSPEMAKEFLKTNETSFSNRPHMVAVDYLSYGSAGFSFAPYGPYWKVMKKLCMSELLGGRTLDQLLPVRREEINRFVKFMLEKAEAGEAVDVGGQLMRSINNVISRMVMGVRCSENEHEADKITKLVKATAELAGKFNLSDFIWFCKNLDLQGFGKRAKKVCDSFDSLMERIIKEREEARKKEKDCAVKDLLHILLDIAEDKNSEMRLTRENIKAFIMDIFVAGTDTTTITIEWALAELIKHPNVLERARQEIDSVVGNSRLVEESDIMNLPYLQAIIKETLRIHPASPIIVRESSEHCTIGGYKIPANTRLFVNVWAIGRDPKHWENPLEFRPERFIISELKESGKSQLDVRGQHFHLLPFGSGRRGCPGTSLALQIVQTTLATLIQCFEWKVVGGDGAVDMEEGPGLTLPRAHRLICSPLARLNPFPSISS
>BPChr14G32249.v1.1.679
IFMAGTDTSSAAMQWAMGELMNHPHAFKTLREEINSVVGLKRLVKESDMPNLPYLRAVIKETLRLHPSAPFIIRECAEDCKVNGSLVKAKTRMLINVYTIMRDPDSWTDPDEFIPERFLESSGERVGEHQMEFKGQNFRYLPFGSGRRGCPGASLAMVVMHPAIGALVQRFDWKGTDGDKIDLRQGSGLAAEMAKPLVC
>BPChr14G12862.v1.1.679
MSDFQDYSLILLLVWLATILVRTFFITKTKAPLPPSPRALPIIGHLHLLSKLPHQSLHKLSNKHGPLIYLLFGSKPCVIVSSPEMARECLKTHETCFLNRPKMTNIDYLTYGSADFAVAPYGPYWKFMKKLCMTELLGGRILEQHISVRGEEIKRFLKLLHDKANANVAVDVGAELIRLTSNIISRMALRKRCSEKEDEAEAVEKLVKEMCELAGKPNVSEMIWFCKNLDLQGFGKRLEDVRGRYDSMMERIMKEREEARRKKKEMGDGGDGIKDVLDILLDIYEDESSEIKMTRENIKGFIMNMFGAGTDTSAVTTEWAISELINHPHLMAKARQEINMVIGKKRIVEESDIANLPYIQAIVKETLRLHPTGPLVVRESTEDCMVSGYEIPAKTRLFVNVWAIGRDPNHWENPLEFWPERFLEKEGSGKSQLDVRGQHFHLLPFGSGRRSCPGASLALQVVQTTLASMVQCFDWKVGDGDQFTVDMEEGPGISLPRAHPLVCVPVARFSPFPSIN
>BPChr14G32246.v1.1.679
VFMAGTDTSSAAMQWAMGELMNHPQAFKKLREEINLVVGPNRLVKESDMPNLPYLRAVIKETLRLHPSAPLIIRECAEDCKVNGSLVKAKTRVLVNVYTIMRDPDSWTDPDEFIPERFLESSGERVGEHQMEFKGQNFRYLPFGSGRRGCPGASLAMVVMHPAIGALVQRFD
>BPChr14G12810.v1.1.679
MAVDVGAELMRLTYNIISRMALRGKCSEKEDEAEEVGELVKEMCELAGKANVSEMIWFCKNLDLQGFGKRLEDVRGRYDSMMERIMKEHEEARRKKKKEIVDGEYVWRWDRHFCCDNGMGNSELINHPHVMAKARQEIDMVIGKKRIVEESDIANLPYIQAIVKETLRLHPTGPIIVRECTDDCIVAGYEIPAKTRLFVNVWAIGRDPNHWENPLEFLPDRFLEKEGSGKRQLDVRGQHFQLLPFGSGRRSCPGATLALQVVQTTLASMVQCFEWKVGDGDDLFTVDMEEGLGVSLPRAHPLVCVPVARFSPFPSIN
>BPChr14G32274.v1.1.679
LLMASVDTLSAAIQWAMAEIINHPQVFKKLRDEINVVVGSTRLVKESDVPNLPYLQAVVKESLRLHSPIPLIFRECTKDCKINGFDVKAKTKTMINAYAIMRDPDYWNDPDEFVPERFLVNSDDKLGQNQMEMKGQDFRYLPFGGGRRGCIGGTHAVLVTQVTLGALTQCFDWKVK
>BPChr14G32311.v1.1.679
LFFASVDTTSAAILWAMTELINHPEVFKKLRVEIESVVGSSRLVKESDVPNLPYLQAVIKEALRIHPPGALLRRQCQQDCNLGGFDIKEGTKILVNIYAIMRDPKSWDEPDKFVPERFLNNNFSDQAFEQMGTKGQDFRFLPFGSGRRACMGEPHASIVLHATIGALIQCFDWELPKDGEKVDVQVVKGYAGAVAHTLVC
LFVAGTDTTSATLEWAMAELLHNTEALSKAKTELEQVIGKGNPMEESDIARLPYLQAVVKETLRLHVPFLLPRKAEADVEINGYIIPKDAQVLVNAWAIGRDPTLWENANSFMPERFL
>BPChr02G32255.v1.1.679
LFVAGTDTTSATLEWAMAELLHNPEILSKVKTELEQVIGKG
>BPChr02G32298.v1.1.679
GTDTTSATLEWAMAELLHNPEALSKARRELEKKIGKGNPVEESDIARLPYLQAIVKETFRLHPAVP
>BPChr08G15423.v1.1.679
MVVLELLVPTLLLALLAAVSTILFSTFQSKLPPSPIALPIIGHFHLLGPLIHQSLYRLSLRYGPLFSLRLGSVPCIVASTPDYAKEFLRNNELSFTSRKHSIAIARLTYNSSFAFAPYGPYWKFMKKLTTNELLGSRTVNAFLSHREKEYRRLLRFLAGKSEGGEAVNVTEELLKLTNNIISQMMLGRAEEARAVGFGKRIEQIHRRFDALVEKVITERQELRRRKENGEHHQLKDFIDILLDFSEDENSETKLTRANIKALIVDFFAAGTDTTAIATEWALAELINHPMMLEKAREEINNVVRNSRLVQESDVPNLPYIQAIIKETLRLHPPLPMVARKCIKDRKIGNYVIPANTLLFVNVWAIGRDPNYWKNPLEFEPERFLQSTKKDSIGLIDVRGQHFQLLPFGSGRRICPGINLAMQELPTLLAAMIQCFDFKLVGPQGEIMDGQNVVVDMEERPGLTAPRAHDLVCVPIARFRPLDILDS
>BPChr10G04396.v1.1.679
MVVLELLVPTLLLALLAAVSTILFSTFQSKLPPSPIALPIIGHFHLLGPLIHQSLYRLSLRYGPLFSLRLGSVPCIVASTPDYAKEFLRNSELSFTSRKHSIAIARLTYNSSFAFAPYGPYWKFMKKLTTNELLGSRTVNAFLSHREKEYRRLLRFLAGKSEGGIWKRIEQIHRRFECFGGEAGTDTTAIATEWALAELINHPMMLEKLVRRSIMLLEIVD
>BPChr10G04362.v1.1.679
MVARKCIKDRKIGNYVIPANTLLFVNVWAIGRDPNYWKNPLEFEPERFLQSTKKDSIGLIDVRGQHFQLLPFGSGRRICPGINWPCKSSNTARSYDPML
>BPChr10G04337.v1.1.679
MALELLVLTILVALLVSTILFSTFRSKLPPSPIALPVIGHFHLLGPLIHQSFHRLALRYGPLFSLRLGSVSCIVASTSDYAREFLKNNELSFISRQHNIAITRLTYNSSLAFAPYGPYWKFIKKLSTNELLGSRTVNMFLSLRDKEYRRFLRFLANKSEAGEAVNVTEELLKLTNNIISQMMLGRAEEARAVVREVTQIFGEFNVSDFIWFCKHLDLQGFGKRIEDISRRFDTLVEKVITERQELRKNKENGKHHHQVKDFIDILLDFSEDENLEIKLTRVHIKALIMDFFTAGTDTTAIATEWALAELINHPIVLEKARKEINNVVNNSRLVQESDVPNLPYIQAIIKETFRLHPPVPMVTRKCIEDCKIGNYVIPANTLLFVNVWAIGRDPNCWKNSLEFEPERFLQLTKKDSMGIIDVRGQHFQLLPFGSGRRICPGINLAMQELPALLAAMIQCFDFKLVGPRGEIIDDQNVVVDMDERPGLTAPRAHDLVCVPIARLSPLDILDV
>BPChr13G32248.v1.1.679
LFIAGTETSAVAMQWAIAELINHPCVLKKVREEIERVVGKSRLVEESDIPNLPYLQAVVKETLRLYPPGPVTTRECRQKCKINGFDIP*KTAVAINLYAIMRDKDSVDDPNKFRPERFLVSLLKDQEKLEQLYQNFNFVPFGAGRRGCPGTTLAFSLMNTAVASLVQCFDWKVDGDGDGGQLV
>BPChr13G07704.v1.1.679
MDILLRVCRDGKAEAKMMTRNHLKAFLVDIFVGGTGTLAEAIIWVMAELINRPNVFHKLREEIRSVVGSTRLVQESDFASLPYLQAVVKEGLRLYPPFPVTTRDCRQSCKIGGFEIPKKTVVLINLYAIMRDPEFWDNPNEFLPERFLVSDEQVQHKHVAETFLSFGAGRRSCPGSKLGLIMMQNAVAAMVQCFDWKFHGDDGHNGNKINMEVGKGVFLHLAQPLTCLPVVHFNPFASSI
>BPChr14G12891.v1.1.679
MTNLDYLTYGSADFVFATYGPYWKFMKKLCMTQLLSGRTLEQNISVRGEEIKRFLQLLHDKAKANVAVDVGAELMRLTYNIISRMALRGKCSEKEDEAEEVGELVKEMCELAGKANVSEMIWFCKNLDLQGFGKRLEDVRGRYDSMMERIMKEHEEARRKKKKEMVDGGDGKKNNMFGGGTDTSAVTMEWAISELINHPHVMAKARQEINMVIGKKRIVEESDIANLPYIQAIVKETLRLHPTGPIIVRECTEDCIVAGYEIPAKTRLFVNVWAIGRDPNHWENPLEFLPDRFLEKEGSGKRQLDVRGQHFQLLPFGSGRRSCPGATLALQVVQTTLASMVQCFEWKVGDGDDLFTVDMEEGLGVSLPRAHPLVCVPVARFSPFPSIN
>BPChr14G32310.v1.1.679
LFFASVDTTSAAILWAMTELINHPEVFKKLRVEIESVVGSSRLVKESDVPNLPYLQAVIKEALRIHPPGALLRRQCQQDCNLGGFDIKEGTKILVNIYAIMRDPKSWDEPDKFVPERFLNNNFSDQAFEQMGTKGQDFRFLPFGSGRRACMGEPHASIVLHATIGALIQCFDWELPKDGEKVDVQVVKGYAGAVAHTLVC
>BPChr14G32277.v1.1.679
WALAELINHPNVMERARQEIDSIVGNSRLVEESDIMNLPYLQAILKETLRIHPTSPIILRESSEHCTIGGYEIPVKIGLFVNVWAIRRDPKHWENPLEF
>BPChr14G12836.v1.1.679
MADLQGYIILFLVSLVSTILVRAIFHRTKTKGRLPPSPMALPIIGHLHHLTPLPHQALHKLSNQYGPLIHLFLGSVPCVVASSPEMAKEFLKTNETSFSNRPHMVAVDYLSYGSAGFSFAPYGPYWKVMKKLCMSELLGGRTLDQLLPVRREEINRFVKFMLEKAEAGEAVDVGGQLMRSINNVISRMVMGVRCSENEHEADKITKLVKATAELAGKFNLSDFIWFCKNLDLQGFGKRAKKVCDSFDSLMERIIKEREEARKKEKDCAVKDLLHILLDIAEDKNSEMRLTRENIKAFIMDIFVAGTDTTTITIEWALAELIKHPNVLERARQEIDSVVGNSRLVEESDIMNLPYLQAIIKETLRIHPASPIIVRESSEHCTIGGYKIPANTRLFVNVWAIGRDPKHWENPLEFRPERFIISELKESGKSQLDVRGQHFHLLPFGSGRRGCPGTSLALQIVQTTLATLIQCFEWKVVGGDGAVDMEEGPGLTLPRAHRLICSPLARLNPFPSISS
>BPChr14G32249.v1.1.679
IFMAGTDTSSAAMQWAMGELMNHPHAFKTLREEINSVVGLKRLVKESDMPNLPYLRAVIKETLRLHPSAPFIIRECAEDCKVNGSLVKAKTRMLINVYTIMRDPDSWTDPDEFIPERFLESSGERVGEHQMEFKGQNFRYLPFGSGRRGCPGASLAMVVMHPAIGALVQRFDWKGTDGDKIDLRQGSGLAAEMAKPLVC
>BPChr14G12862.v1.1.679
MSDFQDYSLILLLVWLATILVRTFFITKTKAPLPPSPRALPIIGHLHLLSKLPHQSLHKLSNKHGPLIYLLFGSKPCVIVSSPEMARECLKTHETCFLNRPKMTNIDYLTYGSADFAVAPYGPYWKFMKKLCMTELLGGRILEQHISVRGEEIKRFLKLLHDKANANVAVDVGAELIRLTSNIISRMALRKRCSEKEDEAEAVEKLVKEMCELAGKPNVSEMIWFCKNLDLQGFGKRLEDVRGRYDSMMERIMKEREEARRKKKEMGDGGDGIKDVLDILLDIYEDESSEIKMTRENIKGFIMNMFGAGTDTSAVTTEWAISELINHPHLMAKARQEINMVIGKKRIVEESDIANLPYIQAIVKETLRLHPTGPLVVRESTEDCMVSGYEIPAKTRLFVNVWAIGRDPNHWENPLEFWPERFLEKEGSGKSQLDVRGQHFHLLPFGSGRRSCPGASLALQVVQTTLASMVQCFDWKVGDGDQFTVDMEEGPGISLPRAHPLVCVPVARFSPFPSIN
>BPChr14G32246.v1.1.679
VFMAGTDTSSAAMQWAMGELMNHPQAFKKLREEINLVVGPNRLVKESDMPNLPYLRAVIKETLRLHPSAPLIIRECAEDCKVNGSLVKAKTRVLVNVYTIMRDPDSWTDPDEFIPERFLESSGERVGEHQMEFKGQNFRYLPFGSGRRGCPGASLAMVVMHPAIGALVQRFD
>BPChr14G12810.v1.1.679
MAVDVGAELMRLTYNIISRMALRGKCSEKEDEAEEVGELVKEMCELAGKANVSEMIWFCKNLDLQGFGKRLEDVRGRYDSMMERIMKEHEEARRKKKKEIVDGEYVWRWDRHFCCDNGMGNSELINHPHVMAKARQEIDMVIGKKRIVEESDIANLPYIQAIVKETLRLHPTGPIIVRECTDDCIVAGYEIPAKTRLFVNVWAIGRDPNHWENPLEFLPDRFLEKEGSGKRQLDVRGQHFQLLPFGSGRRSCPGATLALQVVQTTLASMVQCFEWKVGDGDDLFTVDMEEGLGVSLPRAHPLVCVPVARFSPFPSIN
>BPChr14G32274.v1.1.679
LLMASVDTLSAAIQWAMAEIINHPQVFKKLRDEINVVVGSTRLVKESDVPNLPYLQAVVKESLRLHSPIPLIFRECTKDCKINGFDVKAKTKTMINAYAIMRDPDYWNDPDEFVPERFLVNSDDKLGQNQMEMKGQDFRYLPFGGGRRGCIGGTHAVLVTQVTLGALTQCFDWKVK
>BPChr14G32311.v1.1.679
LFFASVDTTSAAILWAMTELINHPEVFKKLRVEIESVVGSSRLVKESDVPNLPYLQAVIKEALRIHPPGALLRRQCQQDCNLGGFDIKEGTKILVNIYAIMRDPKSWDEPDKFVPERFLNNNFSDQAFEQMGTKGQDFRFLPFGSGRRACMGEPHASIVLHATIGALIQCFDWELPKDGEKVDVQVVKGYAGAVAHTLVC
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University