Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Beta vulgaris
Species name
Beet (Beta vulgaris) is a species of eudicot in the family Chenopodiaceae.
Species info
Gene sequence info
Beta_vulgaris.FLS.fa
Download
>rna-XM_010688817.2
MEVEKVQNIAFKFNKNGGKTSVDSIPLEFIRAENEQPAMTTMHGSVLEVPTIDINDFDEERVVSLISKASEDWGLFQIVNHGIPTQVIHNLQRVGKEFFELPQEEKEVYAKPPPGYQGAEGYGTKLSNKRKEDDVSIRSWVDHLFHNIWPPSAINYHFWPKNPPSYREANEEYAKYLRGVTEKLMKCWSKGLGLEENKMKEAGGGEEMVYLAKINYYPPCPCPELALGVPAHTDLSLLTILVPNDVAGLQVSRDGLWYNVDYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVTKEKTRMSWPVFIEPPAQHQVGPIPELINEQNPANYKTKKFADYKYCKLNKLPQ
>rna-XM_010678781.2
MGEESAIVNLPVIDLAAPDRLVTAATLRQACIEVGFFYIVNHGVDKDLLNRVFEESNKFFSVPLEEKTRLNLKEFRGYTALYSENVDPSSTKGDSKESFYIGPLHNEVCNLNQWPSEELLPSWRPTIEAYHRQILSAGKNLLSLLALTLNLDENFFDKVGAVDPLMPFLRLIHYPGDLRCDNEEVVGAGAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDVPHIEGAIIVNIGDMTERWTNRLFRSTLHRVVPTGQERFSVACFLDPPHNFSVECLDTCCSESNPPRFPGIRYIDYIQESYRNIFEAK
>rna-XM_010678779.2
MEEESGIINLPIIDLSAPDRLTTAATLRQACIGVGFFYIVNHGVEKDLLNRLFEESKKFFSLPLEEKMRLNRKEYRGYTALYSEKLDPSLSTKGDSKESFYIGPLHDEVCNLNQWPSEELLPSWRPIMEEYHRQLLSVGKRLLSLLALALNLDEKFFENVGALDSPMAFLRTIHYPGDISYHNDEVLGASAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDILDIEGAIIVNIGDMTERWTNCLFRSTLHRVVPKGQERYSVAFFLDPPHDFLVECLETACTESNPPRFPPIRFIDYLQERYRLTFGAE
>rna-XM_010684652.1
MNDDLPYMDSNVIDLSLLSSSSMVDNAEEMRKLKSALVSWGCIQLVNHGMSIEFLDEIRKISKQFFELPLEEKEKYSKTSTNPDGYGTDYPNNTYPISYRLFLKIYPENQRSLERWPENPQRFREVLNEYTMKVQKVHDLFAQAMAKSLDLEENTFLNGYGVHSEMILRINYYPPFPLVDRARVMTRHSDASALTFLLKDRKVEALEVLQDDKWFKVPIIPHAIMVIIGDQIEMMSNGIYKSVEHRIRSNPEKARISLALFCSPDLKKEVGPIKELVTNDRPPLYRNVTRYKDSFEKYYIQGLRPISVVKI
>rna-XM_010689556.2
MSVSTTSPMNLKKPSKPVQEIIKTTGKQIPENFIYQLPNKATNHDHSEIKYMDSNIIDLSLLSSSSSNLHEEELRKLRFILSSWGGFQLINHGLSSSLLSQIREVWKEFFALPLEVKQKQCKSNDWYEGYGGDTVSEDQSYNWNDRLRLKTQPPDQRNYNIWPDFVPNFRETLQEYTMEVKRVLEIILKAIAKSINLEENSFFNECGGNEGVHIFTRFNHYPPCSSPSQVLGIKPHSDGSAITILLQDNEVEGLQVEKDKQWFKVPIVPDALFINIGDQLEIMSNGILKSVIHKVVIDKERERISVAMLCAPPPHKEIEPFTELVDKERPLQFNKVKNYFGDLFFQYYPRGERSITTLKF
>rna-XM_010667546.2
MNLTNHQYFFQKQKRSEVMEVGRVQSIANKTASKDTIPLDFIWPENVQPAITTVHGADIEIPLIELDDDDEERLIKLIAKASSEVGLFQIVNHGIPSDVIERFQKVGKEFFELSQEEKERYGKLPGSVEGYGSSLQKEDDGKKGMKKGWVDHIFHRVWPPSAINLRFWPTILTSYREVNEEYAKYLRRVGDKLFTYLSLGLGLERHELREGAGGDDIEYLLKINYYPPCPRPDLALGVPAHSDMSAITLLVPNDVPGLQICKDDRWYNVNYVPNAIVIHIGDQIEIMSNGKYKAVMHRTTVSKDKTRFSWPVFLEPPLEQIVGPISKLINKQNPARFTSQKYRDYSSYKYNNISQQKI
MEVEKVQNIAFKFNKNGGKTSVDSIPLEFIRAENEQPAMTTMHGSVLEVPTIDINDFDEERVVSLISKASEDWGLFQIVNHGIPTQVIHNLQRVGKEFFELPQEEKEVYAKPPPGYQGAEGYGTKLSNKRKEDDVSIRSWVDHLFHNIWPPSAINYHFWPKNPPSYREANEEYAKYLRGVTEKLMKCWSKGLGLEENKMKEAGGGEEMVYLAKINYYPPCPCPELALGVPAHTDLSLLTILVPNDVAGLQVSRDGLWYNVDYIPNALIIHIGDQLEVLSNGKYKAVLHRTTVTKEKTRMSWPVFIEPPAQHQVGPIPELINEQNPANYKTKKFADYKYCKLNKLPQ
>rna-XM_010678781.2
MGEESAIVNLPVIDLAAPDRLVTAATLRQACIEVGFFYIVNHGVDKDLLNRVFEESNKFFSVPLEEKTRLNLKEFRGYTALYSENVDPSSTKGDSKESFYIGPLHNEVCNLNQWPSEELLPSWRPTIEAYHRQILSAGKNLLSLLALTLNLDENFFDKVGAVDPLMPFLRLIHYPGDLRCDNEEVVGAGAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDVPHIEGAIIVNIGDMTERWTNRLFRSTLHRVVPTGQERFSVACFLDPPHNFSVECLDTCCSESNPPRFPGIRYIDYIQESYRNIFEAK
>rna-XM_010678779.2
MEEESGIINLPIIDLSAPDRLTTAATLRQACIGVGFFYIVNHGVEKDLLNRLFEESKKFFSLPLEEKMRLNRKEYRGYTALYSEKLDPSLSTKGDSKESFYIGPLHDEVCNLNQWPSEELLPSWRPIMEEYHRQLLSVGKRLLSLLALALNLDEKFFENVGALDSPMAFLRTIHYPGDISYHNDEVLGASAHSDYGMITLLATDGVPGLQVCREKDRQPRIWEDILDIEGAIIVNIGDMTERWTNCLFRSTLHRVVPKGQERYSVAFFLDPPHDFLVECLETACTESNPPRFPPIRFIDYLQERYRLTFGAE
>rna-XM_010684652.1
MNDDLPYMDSNVIDLSLLSSSSMVDNAEEMRKLKSALVSWGCIQLVNHGMSIEFLDEIRKISKQFFELPLEEKEKYSKTSTNPDGYGTDYPNNTYPISYRLFLKIYPENQRSLERWPENPQRFREVLNEYTMKVQKVHDLFAQAMAKSLDLEENTFLNGYGVHSEMILRINYYPPFPLVDRARVMTRHSDASALTFLLKDRKVEALEVLQDDKWFKVPIIPHAIMVIIGDQIEMMSNGIYKSVEHRIRSNPEKARISLALFCSPDLKKEVGPIKELVTNDRPPLYRNVTRYKDSFEKYYIQGLRPISVVKI
>rna-XM_010689556.2
MSVSTTSPMNLKKPSKPVQEIIKTTGKQIPENFIYQLPNKATNHDHSEIKYMDSNIIDLSLLSSSSSNLHEEELRKLRFILSSWGGFQLINHGLSSSLLSQIREVWKEFFALPLEVKQKQCKSNDWYEGYGGDTVSEDQSYNWNDRLRLKTQPPDQRNYNIWPDFVPNFRETLQEYTMEVKRVLEIILKAIAKSINLEENSFFNECGGNEGVHIFTRFNHYPPCSSPSQVLGIKPHSDGSAITILLQDNEVEGLQVEKDKQWFKVPIVPDALFINIGDQLEIMSNGILKSVIHKVVIDKERERISVAMLCAPPPHKEIEPFTELVDKERPLQFNKVKNYFGDLFFQYYPRGERSITTLKF
>rna-XM_010667546.2
MNLTNHQYFFQKQKRSEVMEVGRVQSIANKTASKDTIPLDFIWPENVQPAITTVHGADIEIPLIELDDDDEERLIKLIAKASSEVGLFQIVNHGIPSDVIERFQKVGKEFFELSQEEKERYGKLPGSVEGYGSSLQKEDDGKKGMKKGWVDHIFHRVWPPSAINLRFWPTILTSYREVNEEYAKYLRRVGDKLFTYLSLGLGLERHELREGAGGDDIEYLLKINYYPPCPRPDLALGVPAHSDMSAITLLVPNDVPGLQICKDDRWYNVNYVPNAIVIHIGDQIEIMSNGKYKAVMHRTTVSKDKTRFSWPVFLEPPLEQIVGPISKLINKQNPARFTSQKYRDYSSYKYNNISQQKI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University