Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Benincasa hispida
Species name
Wax gourd (Benincasa hispida) is a species of eudicot in the family Cucurbitaceae (cucumber family).
Species info
Gene sequence info
Benincasa_hispida.COMT.fa
Download
>Bhi01M002554
MGSWVSDEEAQLFAMQLASASVLPMVLKTAIELDVLEIIGRGGEGAVLSPSQIASQLSGVKNPEAHVMLDRILRLLASYNILTYSLDTLPDGSVQRLYGLAPVCKFLIKNHDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNRAYGMSAFEYHGTDPRFNKVFNRGMSDHSTITMKKILESYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKFLKNCYDALPEHGKVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEKEFQALSKAAGFHGFKLHCCAFNTYVMEFLKTP
>Bhi05M001418
MLITVAPLLVLENSPTIVESWHNLSARLKNNDSTSDLSVPFEVAHGKGIWSYAATNLEFNRMFNEGLGCNARVITLPTILESCGDIFNGIGSLVDVGGGNGTTLSILVKAFPWMKGINFDLPNFVSTSEEYNGVEHVAGNMLHFVPNADAAFFMVSIFICDMLFVCVRGYQ
>Bhi11M000352
MASTAEGTTITSSDDAASQEQHYAYAMELATLSVVPMTLQAAFELGVFEILAKAGNGAELSPAEIAAEITTTNPEAVTMIDRMLRLLASHSVVGCSLAFDEDGNVQRLYSLTPVSKYYVRNEDGVSLGPFLSLLQDKVLLHSWSELKNAVIEGGRAFNRAYGGVHAFEYLGLDPRFNQVFNTAMINHTTMPIKNIVQSYKGFTNIKQLVDVGGGLGVTLHVITSTYPSIKGINFDLPHVIRDAPSYPGVEHVGGDMFEKVPNGDAVFMKWILHDWSDDHCVKLLKNCYTAIPNDGKVIIVDSILPTMPETTSATKAVAQCDMIEMTLYEGGKERTRDEFKALATKAGFKHVIFQCLVSNLWVTEFLKDFTSHQG
>Bhi11M000353
MTLQAAFELGVFEILAKARNGAELSPAEIAAETTTNPEAATMVDRMLRLLASHSVVGYSLAFDEDGNVQRLYSLTPVSKYYVRNEDGVSFGPLLSLLQDKNLLHS
>Bhi11M000350
MPETGKVIIVESVLPTIHETTATMKSIGQMDIIMMTQNPGGKERTQDEFKALATKAGFKHITFNCIVFNLWVMEFSK
>Bhi11M000351
MATINSSDEEHQHFAYAMQLASSSALPMTLQTAFELSVFEILARAGHGVELSPTKIAAEITTANPDEASMIYRILRLLASHSVVGYSVATNQCGNLQTCEHFRSELKNAIVEGGIRTWGAHAFEYPSMDPRSNKIFNTAMSNHATMVVKKIVESYKDFENIKQLVDVGGGLGVTLQIITSTYTSIKGINFDLPHVIQCAPIYPGIHIF
>Bhi11M000354
MASKAEETPITSSDNDAVLEQQHYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGAELSPADIAAEITTKNPDAALMIDRMLWLLASHSVVGFSVGFDEDGKTQRLYSLTPVSKYYVRNEDGVSLGPTLSLIQDKVFLHSWSELKHAVIEGGVPFNRAHGGVHAFEYPGLDPRFNQVFNTGMLNHTTMCIKKLVKTYNGFANIEQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYPGVEHVGGDMFQKVPNGDVVFMKWILHDWSDDHCIKLLKNCYVAIPNDGKVIVVDSVVPTIPETTNVTKSIAQGDVLMMTQNPGGKERTRDEFKAIAIKAGFKHIIFQCFVSNLW
>Bhi11M000355
EIINHASKELVAAASNYSLFYTYQLKQEMDSMAEQTTLPSPSNAAAKEQQHYAYAAQLVTLTVLPMTLQAVFELGVFDILAKAGDGAELSAEQIAGEITNTNPDATSMIDRMLRLLASHMVVGCSVGFDEDGKMQRLYSLNPVSKYYVHNEDGVSVAFNLSLIQDNVFLRSWSELKNAVIEGGIPFNRAHGVHAFEYSSLDPRFNQAFNASMRNHTTMFMKKIVKSYKGFANLKQLVDVGGGLGVGLQIITSTYPSIKGINFDLPHVIQNAPSYPDVEHVGGDMFEKVPKGDAILMKWILHDWSEDRCVKLLKNCYDAIPDDGKVIVVDAVHPTVPETTSAARAVAQGDVLMMTQLLGGRERTRDEFKAIATQAGFKRVIFQSNVCNLWVIELFKFIISH
MGSWVSDEEAQLFAMQLASASVLPMVLKTAIELDVLEIIGRGGEGAVLSPSQIASQLSGVKNPEAHVMLDRILRLLASYNILTYSLDTLPDGSVQRLYGLAPVCKFLIKNHDGVSIAPLCLMNQDKVLMESWYHLKDAVLEGGIPFNRAYGMSAFEYHGTDPRFNKVFNRGMSDHSTITMKKILESYKGFEGLNSVVDVGGGTGAVLNMIVSKYPSIRGINFDLPHVIQDAPAYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDQHCLKFLKNCYDALPEHGKVIVAECILPVAPDSSLATKGVIHIDVIMLAHNPGGKERTEKEFQALSKAAGFHGFKLHCCAFNTYVMEFLKTP
>Bhi05M001418
MLITVAPLLVLENSPTIVESWHNLSARLKNNDSTSDLSVPFEVAHGKGIWSYAATNLEFNRMFNEGLGCNARVITLPTILESCGDIFNGIGSLVDVGGGNGTTLSILVKAFPWMKGINFDLPNFVSTSEEYNGVEHVAGNMLHFVPNADAAFFMVSIFICDMLFVCVRGYQ
>Bhi11M000352
MASTAEGTTITSSDDAASQEQHYAYAMELATLSVVPMTLQAAFELGVFEILAKAGNGAELSPAEIAAEITTTNPEAVTMIDRMLRLLASHSVVGCSLAFDEDGNVQRLYSLTPVSKYYVRNEDGVSLGPFLSLLQDKVLLHSWSELKNAVIEGGRAFNRAYGGVHAFEYLGLDPRFNQVFNTAMINHTTMPIKNIVQSYKGFTNIKQLVDVGGGLGVTLHVITSTYPSIKGINFDLPHVIRDAPSYPGVEHVGGDMFEKVPNGDAVFMKWILHDWSDDHCVKLLKNCYTAIPNDGKVIIVDSILPTMPETTSATKAVAQCDMIEMTLYEGGKERTRDEFKALATKAGFKHVIFQCLVSNLWVTEFLKDFTSHQG
>Bhi11M000353
MTLQAAFELGVFEILAKARNGAELSPAEIAAETTTNPEAATMVDRMLRLLASHSVVGYSLAFDEDGNVQRLYSLTPVSKYYVRNEDGVSFGPLLSLLQDKNLLHS
>Bhi11M000350
MPETGKVIIVESVLPTIHETTATMKSIGQMDIIMMTQNPGGKERTQDEFKALATKAGFKHITFNCIVFNLWVMEFSK
>Bhi11M000351
MATINSSDEEHQHFAYAMQLASSSALPMTLQTAFELSVFEILARAGHGVELSPTKIAAEITTANPDEASMIYRILRLLASHSVVGYSVATNQCGNLQTCEHFRSELKNAIVEGGIRTWGAHAFEYPSMDPRSNKIFNTAMSNHATMVVKKIVESYKDFENIKQLVDVGGGLGVTLQIITSTYTSIKGINFDLPHVIQCAPIYPGIHIF
>Bhi11M000354
MASKAEETPITSSDNDAVLEQQHYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGAELSPADIAAEITTKNPDAALMIDRMLWLLASHSVVGFSVGFDEDGKTQRLYSLTPVSKYYVRNEDGVSLGPTLSLIQDKVFLHSWSELKHAVIEGGVPFNRAHGGVHAFEYPGLDPRFNQVFNTGMLNHTTMCIKKLVKTYNGFANIEQLVDVGGGLGVTLQIITSTYPSIKGINFDLPHVIRDAPSYPGVEHVGGDMFQKVPNGDVVFMKWILHDWSDDHCIKLLKNCYVAIPNDGKVIVVDSVVPTIPETTNVTKSIAQGDVLMMTQNPGGKERTRDEFKAIAIKAGFKHIIFQCFVSNLW
>Bhi11M000355
EIINHASKELVAAASNYSLFYTYQLKQEMDSMAEQTTLPSPSNAAAKEQQHYAYAAQLVTLTVLPMTLQAVFELGVFDILAKAGDGAELSAEQIAGEITNTNPDATSMIDRMLRLLASHMVVGCSVGFDEDGKMQRLYSLNPVSKYYVHNEDGVSVAFNLSLIQDNVFLRSWSELKNAVIEGGIPFNRAHGVHAFEYSSLDPRFNQAFNASMRNHTTMFMKKIVKSYKGFANLKQLVDVGGGLGVGLQIITSTYPSIKGINFDLPHVIQNAPSYPDVEHVGGDMFEKVPKGDAILMKWILHDWSEDRCVKLLKNCYDAIPDDGKVIVVDAVHPTVPETTSAARAVAQGDVLMMTQLLGGRERTRDEFKAIATQAGFKRVIFQSNVCNLWVIELFKFIISH
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University