Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Benincasa hispida
Species name
Wax gourd (Benincasa hispida) is a species of eudicot in the family Cucurbitaceae (cucumber family).
Species info
Gene sequence info
Benincasa_hispida.ROMT.fa
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>Bhi05M001217
MKGTRSHLDTPHKVFGQFLFFVMNMNLEGGNMIEMGGDELLEAQSHIWNHIFNFINSMALKCAIQLGIPDAIHSHGPNPMPLSLLLSSLQLHPNKTQFIYRLMRLLTHSGFFILQEEGYMLTNSSYLLLKDNPYAVSPFVLSMLEPTLLKPWQFLSTWFQTNDRMPFETTHGMPLWEQMRKKPKHGEVFNAGMASDAMLVMNVLLGKHKDVFKGVESLVDVGGGTGTMAKTIAKAFPQIECTVLDLPQVVANLKSDQPNFKYVEGDMFNTIPPADALLLKWILHDWSDEECVKILKKCKEAITSNGKKGKVMVIDLVVFNTKNEDSIETQLFYDMLMMTITSGKEREEKEWAKLIKEAGF
>Bhi05M001219
MEEVLDGNDLLEAQSHVWNQIFIFTKSMALKCAVELGIPDIIHSHGPKPMPLSQLVSALQLNPEKTPYIYRLMRVLVHSGFFLTQKLSEQDGDQEGENHGYVLTNSSRLLVKTNPLTLAPFLFSTLQPPVFDSWSSLPSWLQSEDRTSSPFGSSHGVSFWEYMGRIKSTEGGAIFDASMASDAKFVVRVLLEKCKGVFDGVESLVDVGGGTGNVTRGIAQAFPQIECTVFDLPQVVADLKGEGNLKFVGGDMFGAIPPADALLLKWVLHDWSDEECVKILKKCKEAIKGNEKKGKVMVIDMVVGNNKTDDSIETQLFFDMMVMLMVGGKERDEKEWAHLIKQAGFSAYKILPILGLRSLIEIYP
>Bhi05M001220
MNMNLEGGNMIEMGGDELLEAQSHIWNHIFNFINSMALKCAIQLGIPDAIHSHGPNPMPLSLLLSSLQLHPNKSQFIYRLMRLLTHSGFFILQEEGYMLTNSSRLLLKDNPYAVSPFLFCILEPTFMEPWQFLSTWLHCDDQRTPFETVHGMSFWEYIRNKHKYGDIFNASMASDTMLVVSLLFEKHKGSFEGVRSLVDVGGGTGTMAKAIAKAFPQIECSVLDRPQVVADLKADIPNFKYVEGDMFNAIPPADALVLKWILHDWSDEECVKILKKCKEAITSNGKKGKVMVIDLVVFNTKNKDSIETQLFYDMLMMTMASGKEREEKEWEQLIKESGFSAYKIFPILGLRSLIEIYP
>Bhi05M001222
MDSEGAKMWPVVAGDELLEAEGHIWNHIFNFINSMSLKCAIQLGIPDAIHRHGPSPMALSLLVSSLKLHPNKTQFIYRLMRLLTHSGFFALKKEGYMLTNLSRLLLKDNPCSVTPFLLTMLKPTLTETWQFLSAWFQTNDHTPFEATHGIPFWEYMKSKPRDREAFNTSMANDARLVVSVLLEKYKCVFEGIGSLVDVGGGTGTMAKVIAMAFPQIECTVLDLPHVVVNLKADQPNLTYVEGDMFKVIPPADALLLKVKFKFYCYLQSFEGLIYY
>Bhi05M001223
MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSIKTQLFFDMLMMILAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP
>Bhi05M001225
MDLKDEKMTVFESNNDLLEAQAHIWNHIFDFINSMSLKCAIQLGIPDAIHRHGPNPMPLSLLVSSLKLHPNKTQFIYRLMRLLTYSGFFALQEEGYMLTNSSRLLLKDNPLTLTPFLLSMLDPLIIEPWQLFSACFLNDDKTLFDTAHGMSFWEYVGGRPKDNDVFNAGMASDAKLVMSVLLGKYKGVFEGLGSLVDVGGGTGTMAKAIAEAFPQTVCIVLDLPQVVANMKDNENLKYVGGDMFEAIPPADALLLKWLDTSIYLWIPCLNLYFFSFSSSF
>Bhi09M001202
MEGRNTKMGMEGEEMLRAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLLSSLQIHPRKSQHVRRIMRILVHSGFFLIHNKTTQQGDDDQDQEEAYSLTNPSLLLLQHNPLTLSPFLLFMLDRVLIEPWQFVSAWLLNDTDNRTPFEMAYGKKFWEYMGSETKDGEGFNAAMGSDGRLVMSVLLEKYKSVFEGVECLVDVGGGTGTVAKIIAEAFPQIKCVVFDLPQVVDGLEGNHNLMFLQGDMFQAIPPADALLLKWILHDWTDEECIKILKKCKEAITSNGKIGKVIIIDMVVTNNKKMKNDKLMIECKFFWDLLMMVNVGGKERDEKEWAQLFQLAGFSTYNIFPILGLRSLIELYP
>Bhi09M001204
MEGTNTKMGMEEEEVLKAQAHIWNHIFSFINSMALKCAIELRIPDAISRHGSPITLSQLLSSLQIHPRKSQHVRRIMRILAHSGFFLIQNKTTQQGDDDQDQEEAYSLTNASILLLQHNPLTLSPYLLFVLNHSILHPWQSLSVWFRSNGLDRSPFETAYGKKFWEYMESETRDGEAFNESMARDSKLVMSVLLGNYKSMFEGVESFVDVGGGTGTLAKAMAEAFPQMKCFVFDLPQVVAGLEGNHNLMFLQGDMFQAIPPADALLLKWILHNWNDEECVKILKKCKEAITSNKLKKGKVIIIDMVVTNNNNNNKKADYYSIQTQLFWDMVMMAAVGGMERDEQEWAQLFHKAGFASYNIFPILGLRSLIELYP
>Bhi10M001068
MGENLNELLQSQAHIWSHSLKFINSMSLKCAVELGIPDIIHSHGQPMSLSSLVAALHIEPTKAQCLGRLMRLLVHSGFFTTQTHDHENEEAEDLKYSLTPSSRLLLRHSRSTTFQAIPFLFLCLDKASIASWQCLSSWFCSSNNNGQHYSNAFEMANGKFIWNYAAQEPSFANLFQHTMVCDSEIIGKIVKEECGEVFEGLNSLVDVGGGVGVMGKAIVEAFPHITCTVFDLPHVISNQLLQTQTNLRFVGGDMFEENIPPANAVLLKWVLHNWNDEQSIKILKKCKDAIPSRERGGKLVIIDMVMENKTEDRESTETQLFFDVLMMVNLNGKERTEREWKNLFMKAGFSDYKIISKLGLRSLIEVYP
>Bhi11M001267
MGENKELLQAQAHIWNHTFKCINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPSKAQCLGRLMRLLLHSGFFAQTQSGFFSLTPSSRLLLSQNHKTIFDTKTLLLLFLSPLMMAPWQTMSNWLCASTQDYSTAFQMANGKSIWDYVIQEEESYGFGKLFHQTLVCDSQLIGKVVTSECGEMFEGLSSLVDVGGGAGTMAKVILEAFPHLTCIVLDLPQVVANQKPQQPIKNLNFIEGDMFEKIPPANAVLLKSILHSWNDEESIQILKNCKDAIPSRAEGGRVIIIEMVLDQKERDMESIETQLCFDVLMMTTFSGRERNEREWKTLFLAAGFSDYNIIPILGLRSLIEVYP
>Bhi11M001268
MEDELFLEAQAHIWNHTLKYINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPSKSQPLARLMRLLVHSGFFSQTQQQLVTKYGLTPSSRLLLRRNTTTTTTIETLPFLVLILHQAMMAPWESMSSWLRSEEYGSAFEMVNGKSVWKYIGDEPDFGSLFYQTLVYDSQLIGKLVKRPECREVFDGVKSMVDVGGGEGIVAKAIEEAFPHINCIVLDLPYQTTKDLNVIGGGNLFEPIQIPPTDAVLLVSVLHKYNDEEIIRILKNCKDAITNSNEGGKVIIIEAVLEKEKQMEHKESIETELCGDVLMMATFNTTKRNEKEWKTLFLAAGFSHHKITSFLGLRSLIQLYP
MKGTRSHLDTPHKVFGQFLFFVMNMNLEGGNMIEMGGDELLEAQSHIWNHIFNFINSMALKCAIQLGIPDAIHSHGPNPMPLSLLLSSLQLHPNKTQFIYRLMRLLTHSGFFILQEEGYMLTNSSYLLLKDNPYAVSPFVLSMLEPTLLKPWQFLSTWFQTNDRMPFETTHGMPLWEQMRKKPKHGEVFNAGMASDAMLVMNVLLGKHKDVFKGVESLVDVGGGTGTMAKTIAKAFPQIECTVLDLPQVVANLKSDQPNFKYVEGDMFNTIPPADALLLKWILHDWSDEECVKILKKCKEAITSNGKKGKVMVIDLVVFNTKNEDSIETQLFYDMLMMTITSGKEREEKEWAKLIKEAGF
>Bhi05M001219
MEEVLDGNDLLEAQSHVWNQIFIFTKSMALKCAVELGIPDIIHSHGPKPMPLSQLVSALQLNPEKTPYIYRLMRVLVHSGFFLTQKLSEQDGDQEGENHGYVLTNSSRLLVKTNPLTLAPFLFSTLQPPVFDSWSSLPSWLQSEDRTSSPFGSSHGVSFWEYMGRIKSTEGGAIFDASMASDAKFVVRVLLEKCKGVFDGVESLVDVGGGTGNVTRGIAQAFPQIECTVFDLPQVVADLKGEGNLKFVGGDMFGAIPPADALLLKWVLHDWSDEECVKILKKCKEAIKGNEKKGKVMVIDMVVGNNKTDDSIETQLFFDMMVMLMVGGKERDEKEWAHLIKQAGFSAYKILPILGLRSLIEIYP
>Bhi05M001220
MNMNLEGGNMIEMGGDELLEAQSHIWNHIFNFINSMALKCAIQLGIPDAIHSHGPNPMPLSLLLSSLQLHPNKSQFIYRLMRLLTHSGFFILQEEGYMLTNSSRLLLKDNPYAVSPFLFCILEPTFMEPWQFLSTWLHCDDQRTPFETVHGMSFWEYIRNKHKYGDIFNASMASDTMLVVSLLFEKHKGSFEGVRSLVDVGGGTGTMAKAIAKAFPQIECSVLDRPQVVADLKADIPNFKYVEGDMFNAIPPADALVLKWILHDWSDEECVKILKKCKEAITSNGKKGKVMVIDLVVFNTKNKDSIETQLFYDMLMMTMASGKEREEKEWEQLIKESGFSAYKIFPILGLRSLIEIYP
>Bhi05M001222
MDSEGAKMWPVVAGDELLEAEGHIWNHIFNFINSMSLKCAIQLGIPDAIHRHGPSPMALSLLVSSLKLHPNKTQFIYRLMRLLTHSGFFALKKEGYMLTNLSRLLLKDNPCSVTPFLLTMLKPTLTETWQFLSAWFQTNDHTPFEATHGIPFWEYMKSKPRDREAFNTSMANDARLVVSVLLEKYKCVFEGIGSLVDVGGGTGTMAKVIAMAFPQIECTVLDLPHVVVNLKADQPNLTYVEGDMFKVIPPADALLLKVKFKFYCYLQSFEGLIYY
>Bhi05M001223
MLHGWSDEECVKILKNCKTAITSNGHKGKVIVIDMVVDNKRDENSIKTQLFFDMLMMILAGGKEREEEEWAQLIKEAGFGGYNIIPIMGLRSIIEIYP
>Bhi05M001225
MDLKDEKMTVFESNNDLLEAQAHIWNHIFDFINSMSLKCAIQLGIPDAIHRHGPNPMPLSLLVSSLKLHPNKTQFIYRLMRLLTYSGFFALQEEGYMLTNSSRLLLKDNPLTLTPFLLSMLDPLIIEPWQLFSACFLNDDKTLFDTAHGMSFWEYVGGRPKDNDVFNAGMASDAKLVMSVLLGKYKGVFEGLGSLVDVGGGTGTMAKAIAEAFPQTVCIVLDLPQVVANMKDNENLKYVGGDMFEAIPPADALLLKWLDTSIYLWIPCLNLYFFSFSSSF
>Bhi09M001202
MEGRNTKMGMEGEEMLRAQAHIWNHIFSFINSMALKCAVELRIPDAINRHGSPITLSQLLSSLQIHPRKSQHVRRIMRILVHSGFFLIHNKTTQQGDDDQDQEEAYSLTNPSLLLLQHNPLTLSPFLLFMLDRVLIEPWQFVSAWLLNDTDNRTPFEMAYGKKFWEYMGSETKDGEGFNAAMGSDGRLVMSVLLEKYKSVFEGVECLVDVGGGTGTVAKIIAEAFPQIKCVVFDLPQVVDGLEGNHNLMFLQGDMFQAIPPADALLLKWILHDWTDEECIKILKKCKEAITSNGKIGKVIIIDMVVTNNKKMKNDKLMIECKFFWDLLMMVNVGGKERDEKEWAQLFQLAGFSTYNIFPILGLRSLIELYP
>Bhi09M001204
MEGTNTKMGMEEEEVLKAQAHIWNHIFSFINSMALKCAIELRIPDAISRHGSPITLSQLLSSLQIHPRKSQHVRRIMRILAHSGFFLIQNKTTQQGDDDQDQEEAYSLTNASILLLQHNPLTLSPYLLFVLNHSILHPWQSLSVWFRSNGLDRSPFETAYGKKFWEYMESETRDGEAFNESMARDSKLVMSVLLGNYKSMFEGVESFVDVGGGTGTLAKAMAEAFPQMKCFVFDLPQVVAGLEGNHNLMFLQGDMFQAIPPADALLLKWILHNWNDEECVKILKKCKEAITSNKLKKGKVIIIDMVVTNNNNNNKKADYYSIQTQLFWDMVMMAAVGGMERDEQEWAQLFHKAGFASYNIFPILGLRSLIELYP
>Bhi10M001068
MGENLNELLQSQAHIWSHSLKFINSMSLKCAVELGIPDIIHSHGQPMSLSSLVAALHIEPTKAQCLGRLMRLLVHSGFFTTQTHDHENEEAEDLKYSLTPSSRLLLRHSRSTTFQAIPFLFLCLDKASIASWQCLSSWFCSSNNNGQHYSNAFEMANGKFIWNYAAQEPSFANLFQHTMVCDSEIIGKIVKEECGEVFEGLNSLVDVGGGVGVMGKAIVEAFPHITCTVFDLPHVISNQLLQTQTNLRFVGGDMFEENIPPANAVLLKWVLHNWNDEQSIKILKKCKDAIPSRERGGKLVIIDMVMENKTEDRESTETQLFFDVLMMVNLNGKERTEREWKNLFMKAGFSDYKIISKLGLRSLIEVYP
>Bhi11M001267
MGENKELLQAQAHIWNHTFKCINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPSKAQCLGRLMRLLLHSGFFAQTQSGFFSLTPSSRLLLSQNHKTIFDTKTLLLLFLSPLMMAPWQTMSNWLCASTQDYSTAFQMANGKSIWDYVIQEEESYGFGKLFHQTLVCDSQLIGKVVTSECGEMFEGLSSLVDVGGGAGTMAKVILEAFPHLTCIVLDLPQVVANQKPQQPIKNLNFIEGDMFEKIPPANAVLLKSILHSWNDEESIQILKNCKDAIPSRAEGGRVIIIEMVLDQKERDMESIETQLCFDVLMMTTFSGRERNEREWKTLFLAAGFSDYNIIPILGLRSLIEVYP
>Bhi11M001268
MEDELFLEAQAHIWNHTLKYINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPSKSQPLARLMRLLVHSGFFSQTQQQLVTKYGLTPSSRLLLRRNTTTTTTIETLPFLVLILHQAMMAPWESMSSWLRSEEYGSAFEMVNGKSVWKYIGDEPDFGSLFYQTLVYDSQLIGKLVKRPECREVFDGVKSMVDVGGGEGIVAKAIEEAFPHINCIVLDLPYQTTKDLNVIGGGNLFEPIQIPPTDAVLLVSVLHKYNDEEIIRILKNCKDAITNSNEGGKVIIIEAVLEKEKQMEHKESIETELCGDVLMMATFNTTKRNEKEWKTLFLAAGFSHHKITSFLGLRSLIQLYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University