Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Averrhoa carambola
Species name
Averrhoa carambola is a species of eudicot in the family Oxalidaceae (wood-sorrel family).
Species info
Gene sequence info
Averrhoa_carambola.ANR.fa
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>Yangtao2024529
MSGGTAKEKEVCVTGASGYRATWLVKLLLQRGYTVKASVRDPNDPKKTEHLLALDGAKEKLHLFKADLLDEGSSDYLVDGCEGVLHTASPFYFTVTDAQTELIEPAVKGTLNVLISCVKVPSIKREVITSSMAAVVFNGRPWFSDAVFCEESKVCQCSDSLA
>Yangtao2022483
MAKVELIDPVVKGTLNVLGSCTKASSIKIVVLTSSMSSVVFNERLLAPNVVVDETWFSDPNYCKE
>Yangtao2022485
MSGGTAKKEKVVCVTGASGYIASWLVKLLLQRGYTVKASVRDPNDPKKMEHLLALDGAKEKLHLFKADLLDEGSFDSLVDGCECVFHTASPFYFTVTDAQTELIDPAVKGTLNVLRSCVKVPSIKRVVITSSMAAVVFNGKPLAPDVLVDESWFSDAVFCEKSKTLAEEAAWKFAKENRIDVVALNPGLVIGPLLQPTVNTSVEPILKLVNGAETFPNTTFRFVDVRDVANAHILAFENSSACGRYCLVSNVIHCSELLKILRALFPALTLPEKCSDDHPPTPLYQVSKVRVESLGINYTPLEFLLQRGYTVKATVRDPNDPKKTEHLSALDGAKERLHLFQADLMEEGVFDCVVDGCEAVFHTASPVLLSVTDPQAELIDPAVKGTLNVLRSCAKCSSIKRVVVTSSMASVAFNAKPKAPDLVIDETWFSDKSFCKESKLWYVLAKTLAEEAAWKFAKENGIDLVTINPGYVIGPLLQRTLNLTVEMIFKLVNGAQTFSNVDYRFVDVRDVAYAHIQAYEIPSTSGRYCLVGIVTHLSEAVKILKELYPSLQLPEIRSTEESRAPPLYRVSKEKAKSLGINFTPLEDMLRDTVESFKEKGFLSV
>Yangtao2022494
MSRGPMIKFICSKQFKLIRFVDVRDVANARILVFENSSACGRYCLVSNVIHCSELVKILRALFPALTLPEKCSDDHPPTPLYQVSKVRVESLGINYTPLEMFEDIVDQSQWQRKCETTSILSGYVYGTDDRKKTERLRALDGAKERLHLFKADLMEDGVFDSVIDGCEAGFLTASRVLLSVTDPQAELTDPTVKGTLNVLRSCAKTSSIKRIVVTSSMASVAFNGKPKTPDLLWYALAKTLAEEAAWKFAKENWIDLVTINPGYVIGPLLPPTLNLTVEMIFKLVNGAQTFSNADYRFVDVRDVAYTHIQAYEIPSTSGRYCLVGTINHLSEAVNSERTLSFSATS
>Yangtao2007731
MASEKNKVCVVGGSGFLASLLVKLLLEKGYAVNCTVRNPDDEKNSHLIALKELGDLQIFRADLTDEGSFDAPVADCQFVFQVATPVNFTSKDPENDVIKPAVQGVLNVMAACTKAKTVKRFIYTSSAAAVAINTLEGTGIVVDENNWTDVEFLRLAKPHAWGYSVSKTLSEKAAWKFAEENNIDLITVIPNILLGPSLTPHIPNSIGLCTSLMTGNEFHLNGLKGMQKLSGSISIVHVEDVCRAQIFLAEKQSTSGRYICCAANTSVPELAKFLSNRYPQYVVPSNFGDLPAKAKLIVSSEKLIKEGFNYKYGIEETYDQTVEYAKAKGLLN
>Yangtao2017221
MEKNSFYCKVCVTGGFGFLGSSLIKKLLDRGYTVHATLRNLEDKAKVELLKTLPKADTNLVLFQADLYNPTEFEAAINGCEFVFHVATPLLHNHQSTQFKDTTEAAIAGVRSIVESCIRSKSVKRLIYTGSVMASSPLTEDLEFKSCLDESCWTPLNISLVTESDEYVLDYVKSKTLAEKTALSYNDTRDYNLEVVTLSCGLVGGDTLLPNVAASIAEIISQITGNLSLYKILKFLEELLGSIPLVHIDDICEAHVFCMEKQSMRGKFLCAATSTTIRDIAVYLQENYPEYHIAEEFMGEEKQSIGCDSTKLIKMGFRYKYNMKGIIDDSVDCGRRLGAFSV
>Yangtao2010942
MVVSSFCKVCVTGDDSSKVDLLKSFPGADERLVSFQADIYSPNEFEKAIEGCKFVFHVATPLQHTEGHQFKNIVEAAVTGAKSIESSCVRSGTVKRLIYTGSVVAASPWKDDGTGFKDSMDETCWTLLHLPFAYSTDHLQAYTESKTQTEKELLSDGNKGDGGGLMEVVALNCGLVGGDTLLSYTPLSVAVFVPQLTNNEVNYHSLRYLEELLGKVSIVHIDDVCEAHIFCAENPSTNGRFLCASSYVSSVEIADYYQQNYPEFHVKPEQVYSYILFSL
MSGGTAKEKEVCVTGASGYRATWLVKLLLQRGYTVKASVRDPNDPKKTEHLLALDGAKEKLHLFKADLLDEGSSDYLVDGCEGVLHTASPFYFTVTDAQTELIEPAVKGTLNVLISCVKVPSIKREVITSSMAAVVFNGRPWFSDAVFCEESKVCQCSDSLA
>Yangtao2022483
MAKVELIDPVVKGTLNVLGSCTKASSIKIVVLTSSMSSVVFNERLLAPNVVVDETWFSDPNYCKE
>Yangtao2022485
MSGGTAKKEKVVCVTGASGYIASWLVKLLLQRGYTVKASVRDPNDPKKMEHLLALDGAKEKLHLFKADLLDEGSFDSLVDGCECVFHTASPFYFTVTDAQTELIDPAVKGTLNVLRSCVKVPSIKRVVITSSMAAVVFNGKPLAPDVLVDESWFSDAVFCEKSKTLAEEAAWKFAKENRIDVVALNPGLVIGPLLQPTVNTSVEPILKLVNGAETFPNTTFRFVDVRDVANAHILAFENSSACGRYCLVSNVIHCSELLKILRALFPALTLPEKCSDDHPPTPLYQVSKVRVESLGINYTPLEFLLQRGYTVKATVRDPNDPKKTEHLSALDGAKERLHLFQADLMEEGVFDCVVDGCEAVFHTASPVLLSVTDPQAELIDPAVKGTLNVLRSCAKCSSIKRVVVTSSMASVAFNAKPKAPDLVIDETWFSDKSFCKESKLWYVLAKTLAEEAAWKFAKENGIDLVTINPGYVIGPLLQRTLNLTVEMIFKLVNGAQTFSNVDYRFVDVRDVAYAHIQAYEIPSTSGRYCLVGIVTHLSEAVKILKELYPSLQLPEIRSTEESRAPPLYRVSKEKAKSLGINFTPLEDMLRDTVESFKEKGFLSV
>Yangtao2022494
MSRGPMIKFICSKQFKLIRFVDVRDVANARILVFENSSACGRYCLVSNVIHCSELVKILRALFPALTLPEKCSDDHPPTPLYQVSKVRVESLGINYTPLEMFEDIVDQSQWQRKCETTSILSGYVYGTDDRKKTERLRALDGAKERLHLFKADLMEDGVFDSVIDGCEAGFLTASRVLLSVTDPQAELTDPTVKGTLNVLRSCAKTSSIKRIVVTSSMASVAFNGKPKTPDLLWYALAKTLAEEAAWKFAKENWIDLVTINPGYVIGPLLPPTLNLTVEMIFKLVNGAQTFSNADYRFVDVRDVAYTHIQAYEIPSTSGRYCLVGTINHLSEAVNSERTLSFSATS
>Yangtao2007731
MASEKNKVCVVGGSGFLASLLVKLLLEKGYAVNCTVRNPDDEKNSHLIALKELGDLQIFRADLTDEGSFDAPVADCQFVFQVATPVNFTSKDPENDVIKPAVQGVLNVMAACTKAKTVKRFIYTSSAAAVAINTLEGTGIVVDENNWTDVEFLRLAKPHAWGYSVSKTLSEKAAWKFAEENNIDLITVIPNILLGPSLTPHIPNSIGLCTSLMTGNEFHLNGLKGMQKLSGSISIVHVEDVCRAQIFLAEKQSTSGRYICCAANTSVPELAKFLSNRYPQYVVPSNFGDLPAKAKLIVSSEKLIKEGFNYKYGIEETYDQTVEYAKAKGLLN
>Yangtao2017221
MEKNSFYCKVCVTGGFGFLGSSLIKKLLDRGYTVHATLRNLEDKAKVELLKTLPKADTNLVLFQADLYNPTEFEAAINGCEFVFHVATPLLHNHQSTQFKDTTEAAIAGVRSIVESCIRSKSVKRLIYTGSVMASSPLTEDLEFKSCLDESCWTPLNISLVTESDEYVLDYVKSKTLAEKTALSYNDTRDYNLEVVTLSCGLVGGDTLLPNVAASIAEIISQITGNLSLYKILKFLEELLGSIPLVHIDDICEAHVFCMEKQSMRGKFLCAATSTTIRDIAVYLQENYPEYHIAEEFMGEEKQSIGCDSTKLIKMGFRYKYNMKGIIDDSVDCGRRLGAFSV
>Yangtao2010942
MVVSSFCKVCVTGDDSSKVDLLKSFPGADERLVSFQADIYSPNEFEKAIEGCKFVFHVATPLQHTEGHQFKNIVEAAVTGAKSIESSCVRSGTVKRLIYTGSVVAASPWKDDGTGFKDSMDETCWTLLHLPFAYSTDHLQAYTESKTQTEKELLSDGNKGDGGGLMEVVALNCGLVGGDTLLSYTPLSVAVFVPQLTNNEVNYHSLRYLEELLGKVSIVHIDDVCEAHIFCAENPSTNGRFLCASSYVSSVEIADYYQQNYPEFHVKPEQVYSYILFSL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University