Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Xanthoceras sorbifolium
Species name
Yellow-horn (Xanthoceras sorbifolium) is a species of eudicot in the family Sapindaceae (soapberry family).
Species info
Gene sequence info
Xanthoceras_sorbifolium.FLS.fa
Download
>XS02G09096.1
MDRVVDLEALLMVAFRLLMLSSSWTFTSRRGTDRQQLKNISKNRLKMKTKNRSKTKTKKRSKNPEDEEQIEDEGIALKYKEKIPEEFVRSKDEQPGITTVKGAVFEVPVIDLSNPDEEKILQSIVDASQKWGMFQVVEHRIPGEIISKLQQVGREFFELTEEEKEVYAKPLDDGTLEGYGTKLQKEVHGKKGWVDHLFHKIWPLSAVNYQFWPKKPSSYREANEEYAKWVQGIGDKLLKWLSLGLGLEEDDVKEALGGENLVYLMKINYYPPCPQPEVALGVVAHTDMNIITILVPNDVQGLQAFRDGLWYDVNYIPNALVIHIGDQMEILSNGRYKSVLHRTRVNKEKTRMSWPVFLEPPADLQVGPHTKLVNKNNPAKYKTKKYSDYVYCKLNKIPQKGQFVVS
>XS05G15000.1
MEVERVQALSYGGLNELPAQFIRPTHERPENSKPIEGVKVPVISLSQPDHHVLVKDIFEACNQWGFFLITDHGISPSLIQRLQEVGQEFFKLPKEEKEAYANDPSSGKFEGYGTKMTKNQDEKVEWVDYFFHLMTPKTKINYDHWPKNPPSYREVTDAYNKEILRITYQLLELLSEGLGLSGKVLESRLGNEEIELEMKINMYPPCPQPQLALGVEPHTDMSALTILVSNDVPGLQLWKDGNWVAVNYLPNALFVHVGDQLEVMHLLLFNSITLRNS*GSHRRIQ*GNFKDNIPTTGVALRRARPVREGFGVPLGK*RDRTRNENKYVSTMPTTSTGPWS*TSY*HVCTHHTCLQRCSRSSTLERWQLGCSQLLAKCTFCPCWRSTRGFEQWQVQECSSQECSEQGAYAHVLGSLLHSTTRGIDWTTSRTCRRPESSQILNQNLCGISIPQIQQAPTV
>XS07G18516.1
MAVESAQAVASICKNLKGSIPAEFIRPEIEQPAITTYHGKVPEIPTIDLSDPDEEKVVRLMAEASREWGIFQVVNHGIPNDVISRLQAVGKEFFELPLHEKEAYSKPPGSLSHEGYGSKLQKEVDGKKSWVDHLFHKIWPPSSINYKFWPKIPPSYREVNEEYAKYMREVVEKLFRGLSIGLGLGGHRLKEAAGGEEIEYMLKINYYPPCPRPDLALGVVAHTDLSTLTILVPNEVPGLQVFKDDRWIDAKYIPNALIVHIGDQIQVIFLSLSLNYEYV*GSE*RIRKIHERGGGEVVQGAVNRIRSWRT*T*RSSRR*GD*IHVEDKLLPTMSSSRSRTRCGGPY*SFYSHHSCSQ*SSWSPSLQG*PLDRRQVHPQRSHCSHW*SDSDSEQRQV*GGATQDNS**GKDEDVMASFPRTTK*AGRGSNC*SS*RRRKSSQI*SQKIQGLYVLQTQQNSSV
>XS08G19054.1
MAQLSPLPAEILLSKRVQEMSLNGEQPPPLYICRHADTVEEVSAQLSPLSIIDLSLFSSSTPCTKQQQELEKLRSALCSWGCFQAIGHGISDTFLDKVRQVTREYFKQPMEEKKKQAKKVEEFEGYGADPVPAEGQFLDWSDRLFLDVYPEDRRKLVFWPERPESFRGILEEYSLKMKMVTELVSKAMANSLNLEENCFLNQYGERAQYQARFNYYSCCQRPDLVLGLKPHADGSGYTIIMQDEEGLQVLKDEKWFTVPKISEAVLVLMGDQMERYLRRILS*DEDGDRACFESHGKFTEFRRKLLLESIWGKSPVPS*V*LLLMLSEA*SCSWLKTTCRWIRIHYYNAG*RRSSSTQR*EMVYSTQNIRSCPSPHGRSNGGIYVNNDQWYIQKPRA*GGD*NRKGENFSGSFLYTRSE*RDWTRRWLNQ*GKTENIQEGERLC*YTLGILPARTESATYCKSV
>XS08G19451.1
MFVTNIFNSMANSPQSMPEKPLDFRAPPPSPIASSRRSSVTNDEVLTEFLEHSLRVPDLILPDKVFPRQKFIENPPRIDFQSLNSKESDSVVSRIVDSITTIGCFQLVNYGIPSEVIKSTSSAAAGIFRLPSDKRAAIRRSPERAYGFEEVHWEEEEEDDDNDSELSEEFVWCRNECLKLEMEGIWPLEYSKFSEKMEALLMEIEKVGEKILQVLKENAMRKSTYGDHEHENVGSVCYLYRHDRNNIPEDRRANSLQYDVIRMLVRGVDISHSLSLHVCDGASEFHVYSKKGWVSFCPDKDALVVTAGDQLQALSAGQYKHVIGRPIFEADKEDCISMAFLYSPPTTTTNNATTTNSNNKGKTISLAQQLTVAIFVTLLYHFLVYYYKKF
MDRVVDLEALLMVAFRLLMLSSSWTFTSRRGTDRQQLKNISKNRLKMKTKNRSKTKTKKRSKNPEDEEQIEDEGIALKYKEKIPEEFVRSKDEQPGITTVKGAVFEVPVIDLSNPDEEKILQSIVDASQKWGMFQVVEHRIPGEIISKLQQVGREFFELTEEEKEVYAKPLDDGTLEGYGTKLQKEVHGKKGWVDHLFHKIWPLSAVNYQFWPKKPSSYREANEEYAKWVQGIGDKLLKWLSLGLGLEEDDVKEALGGENLVYLMKINYYPPCPQPEVALGVVAHTDMNIITILVPNDVQGLQAFRDGLWYDVNYIPNALVIHIGDQMEILSNGRYKSVLHRTRVNKEKTRMSWPVFLEPPADLQVGPHTKLVNKNNPAKYKTKKYSDYVYCKLNKIPQKGQFVVS
>XS05G15000.1
MEVERVQALSYGGLNELPAQFIRPTHERPENSKPIEGVKVPVISLSQPDHHVLVKDIFEACNQWGFFLITDHGISPSLIQRLQEVGQEFFKLPKEEKEAYANDPSSGKFEGYGTKMTKNQDEKVEWVDYFFHLMTPKTKINYDHWPKNPPSYREVTDAYNKEILRITYQLLELLSEGLGLSGKVLESRLGNEEIELEMKINMYPPCPQPQLALGVEPHTDMSALTILVSNDVPGLQLWKDGNWVAVNYLPNALFVHVGDQLEVMHLLLFNSITLRNS*GSHRRIQ*GNFKDNIPTTGVALRRARPVREGFGVPLGK*RDRTRNENKYVSTMPTTSTGPWS*TSY*HVCTHHTCLQRCSRSSTLERWQLGCSQLLAKCTFCPCWRSTRGFEQWQVQECSSQECSEQGAYAHVLGSLLHSTTRGIDWTTSRTCRRPESSQILNQNLCGISIPQIQQAPTV
>XS07G18516.1
MAVESAQAVASICKNLKGSIPAEFIRPEIEQPAITTYHGKVPEIPTIDLSDPDEEKVVRLMAEASREWGIFQVVNHGIPNDVISRLQAVGKEFFELPLHEKEAYSKPPGSLSHEGYGSKLQKEVDGKKSWVDHLFHKIWPPSSINYKFWPKIPPSYREVNEEYAKYMREVVEKLFRGLSIGLGLGGHRLKEAAGGEEIEYMLKINYYPPCPRPDLALGVVAHTDLSTLTILVPNEVPGLQVFKDDRWIDAKYIPNALIVHIGDQIQVIFLSLSLNYEYV*GSE*RIRKIHERGGGEVVQGAVNRIRSWRT*T*RSSRR*GD*IHVEDKLLPTMSSSRSRTRCGGPY*SFYSHHSCSQ*SSWSPSLQG*PLDRRQVHPQRSHCSHW*SDSDSEQRQV*GGATQDNS**GKDEDVMASFPRTTK*AGRGSNC*SS*RRRKSSQI*SQKIQGLYVLQTQQNSSV
>XS08G19054.1
MAQLSPLPAEILLSKRVQEMSLNGEQPPPLYICRHADTVEEVSAQLSPLSIIDLSLFSSSTPCTKQQQELEKLRSALCSWGCFQAIGHGISDTFLDKVRQVTREYFKQPMEEKKKQAKKVEEFEGYGADPVPAEGQFLDWSDRLFLDVYPEDRRKLVFWPERPESFRGILEEYSLKMKMVTELVSKAMANSLNLEENCFLNQYGERAQYQARFNYYSCCQRPDLVLGLKPHADGSGYTIIMQDEEGLQVLKDEKWFTVPKISEAVLVLMGDQMERYLRRILS*DEDGDRACFESHGKFTEFRRKLLLESIWGKSPVPS*V*LLLMLSEA*SCSWLKTTCRWIRIHYYNAG*RRSSSTQR*EMVYSTQNIRSCPSPHGRSNGGIYVNNDQWYIQKPRA*GGD*NRKGENFSGSFLYTRSE*RDWTRRWLNQ*GKTENIQEGERLC*YTLGILPARTESATYCKSV
>XS08G19451.1
MFVTNIFNSMANSPQSMPEKPLDFRAPPPSPIASSRRSSVTNDEVLTEFLEHSLRVPDLILPDKVFPRQKFIENPPRIDFQSLNSKESDSVVSRIVDSITTIGCFQLVNYGIPSEVIKSTSSAAAGIFRLPSDKRAAIRRSPERAYGFEEVHWEEEEEDDDNDSELSEEFVWCRNECLKLEMEGIWPLEYSKFSEKMEALLMEIEKVGEKILQVLKENAMRKSTYGDHEHENVGSVCYLYRHDRNNIPEDRRANSLQYDVIRMLVRGVDISHSLSLHVCDGASEFHVYSKKGWVSFCPDKDALVVTAGDQLQALSAGQYKHVIGRPIFEADKEDCISMAFLYSPPTTTTNNATTTNSNNKGKTISLAQQLTVAIFVTLLYHFLVYYYKKF
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University