Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Welwitschia mirabilis
Species name
Welwitschia mirabilis is a species of seed plant in the family Welwitschiaceae.
Species info
Gene sequence info
Welwitschia_mirabilis.ANR.fa
Download
>W.mirabilis.20446
MKLSRASVTLKTHRRRRSQERRILWEARCRRVFNILKSCHASRSVHKVLITSSATMLSCRPEFEKTGHVDGSSSSDMQSLRSEKPCRILLPKSRQRRPRMRTISSR
>W.mirabilis.20447
MRRIFMDPHKEMQKKATDEAQQSFGYLEDAQKTTFAGKAYFVGSPLSKGFFNILKSCHASRSVHKVLITSSATMLSCRPEFEKTGHVDGSSSSDMQSLRSEKPCRILLPKSRQRRPRMRTISSR
>W.mirabilis.00616
MDATAVSESKSKVCVTGASGYIASNLVSLLLRSGYSVNATVRDPENPSKVQHLLKLPGAQSRLHLFKADLFEEGSFDAAIAGCEGVFHVATPANFKPKDPENEVIKPAVKGTLDILESCRKSGTVKRVVYTSSVAALVWKQEDQKHEYINESSWSDLQFIRNSKPPAWPYVASKTEAERAALDFGREHGLHVITVVPVVVVGPWLTPSISASVALAMSPLTGKPHSLSNLKGFQMSTGTVPLIHVDDVSESHVFLYETPSASGRYVCCAQSITLSELGRLMRERYPQSYKGPTDFSDAEPTRELTVSSKKLIDAGFRFRHRDVASMIDDTVECMRAKGYLS
>W.mirabilis.08761
MQWRKEHAQQRLNINALPKSVRFAGVTDTRIAAFSILNSFIFLVYRISSQFSFLSIVYVDISQTWTLCAEASTKKPKVCVTGASGYVASALVKLLLRKGYSVHATVRDPENRSKVEHLLRLPGADAGLHLFRADLLEEGSFDAAVAGCEGVFHVASPLSFHPTDPEDDVIKPAVKGTLNILKSCRDSTSVRRVVITSSASTLPWKAEREKTVYVDESAWSDSQFLRSEKPAGWPYYVAKTEAEKAALEFGYENGLDVLTLVASVLVGPWLTSSMPASVGMVMSLLTPDPSSIGYLIAIQMGCGTVPLVHVDDICEAHVFIYEMSTCSGRYVCCASNIKLSELARHVCKKYPMICREAMDLRHVEAPFKIHISSRRLMEVGFQYRHTDVDSMLDDTVECMRGTLNILKSCRDSTSARRVVITSSASTLPWKAEREKTAYVDESAWSDSQFLRSEKPAGWPYFVAKTEAEKAALEFGYENGLDVLTLVAVLVVGPWLTSSIPASVGMVMSLLTPDPSSIGNLIAIQMGCGTVPLVHVDDICEAHVFIYEMSTCSGRYVCCASNIKLSELARHVCKKYPMICREAMDFRHVEAPFKIHISSRRLMDVGFQYRHTDVDSMLDDTVECMRGKGLLL
>W.mirabilis.08762
MDATAVSESKSRVCVTGASGYVASNLVSLLLRSGYCVNATVRDPVNPSKVQHLLKLPGAESRLRLFKADLFEEGSFDAAIAGCEGVFHLATPANFEPKDPENEVIQPTVKGTLDILESCRKSASVKRVVYTSSAATLTWKEENQKHEYIDESSWSDLQFIRNNKPPAWPYLVSKTEAERAALRFGREHGLHVITVVPVLVVGPWLTPSIPGSVAAAMSPLTGEPHFLYHLKGVQMSTGTVPLVHVDDVSETHVFVYETPSASGRHVCCAQGITLSELGRLMRERYPQSYKGPTDFSDAEPTRELTISSKKLMDAGFRFRRCQVASMIDDTVECMRAKGYLS
>W.mirabilis.08763
MDATAVSESKSKVCVTGASGYIASNLVSLLLRSGYSVNATVRDPENPSKVQHLLKLPGAKSRLHLFKADLFEEASFDAAIAGCEGVFHVATPSNFKPKDPENEVIKPAVKGTLDILESCRKSGTVKRVVYTSSAAALVWKQEDQKHEYINESSWSDLQFIRNSKPPAWPYVASKTEAERAALDFGRPVLGLSKPGPISS
>W.mirabilis.25660
MDAAATLESESKPTVCVTGVSGYIASSLVSLLLRLGYSIHGTVRNPENTSKTQHLMKLPGADKRLRIFKADLCEEGSYDAAVAGCQGLFHVAAPVDPNPNDPENEVIKVGIKGTLDVLESCRKTGSVKRVVLMSSFGALFWKEEDDGTNYLDELSSSDVDFLRRKKPPAWAYWIAKTESEKAALEFGRKNGMEVATLTPTLVVGRWITPSVPGSVLVATSLLTGHPGVAFLKAFNEACVRVELVHVDDVCAACVFVYETQSSIGRHVCCPVGIDLPALARRMRERYADSYVGPTDFDCMGPTGHVTFSCKKLTDMGFRFQYADVDSMMDDTAYWIAKTESEKAALEFGRKNGMEVATLTPPLVVGRWITPSVPGSVLVATSLLTGHPGVVFLKAFNEACVRVELVHVDDVCAACVFVYETQSSIGRHVCCPVGIDLPALARRMRERYADSYVGPTDFDCMGPTGHVTFSCKKLTDMGFRFQYADVDSMMDDTVKCMRVNGLLP
>W.mirabilis.09150
MQSASNYSFIGHLYSCSFVATLTWKKENQKHKYIDASSWSDFQFIRNNKPLARPYTVPKTEAEKESLHLGREHDLCIITVVPVLSVAAAMSPLTGKTHFLYDLEGVQISAGTMSLVHVDNIADR
>W.mirabilis.17816
MRVACVTGAGGFIASWIVKYLLDGGYSVHGTLRNPNDEKNSHLQALNGAKERLKLFKADVLAYESVLQAIDGCDTVFHTACPVPKTESWYLYAKTVSEQKAWEFCKEKGIHLVSLCPSLVLGPLLQKQLNFSSSILLKLVNGETEKCENTNRNCIDVRDVAKAHILAAEVPSAHGRYLCTCHFIDTKGMVDILRELYPQKTESWYLYAKTVSEQKAWEFCKEKGIHLVSLCPSLVLGPLLQKHLNFSSSILLKLVNGETEKCENTNRNCIDVRDVAKAHILAAEVPSAHGRYLCTCHFIDTKGMVDILRELYPQYDYPKEFVDTDQWSLKRASCSRLRQLENSTATTIKYYVVMKVV
>W.mirabilis.19214
MDATAISKSRSEVCMTDTSGHIASNLVSLLLCSEFSVNTTVHDPENPSKMQHLPNYLKRKIVFIFSKRISFRKGALMLQSPVVKESSMSSLHSTLSLKIPPKIFMAYLSITFTEDALQKKKSSTLLKVSQNGGLRR
>W.mirabilis.19215
MDATAISKSRSEVCMTDTSGYIASNLVSLLLCSEFSVNTTVHDPENPSKMQHLPNYLKRKIVFIFSKRISFRKGALMLQSPVVKESSMSSLHSTLSLKIPPKIFMAYLSITFTEDALQKKNPPRY
>W.mirabilis.19815
MLGPLLQKQLNSSSLILLKLLNGEMKKRENKIWNCIAIIEGCDTVFQTACPVGIFSNPEEEMVKPSVEGTLNVLKACSLSKVRRVIFTSSLTAIRFTPKIPGDKVLMKVAGRIWNIAGRLRIGTFTRRQWQSKRHGSFKQLNSSSLILLKLLNGEMKKRENKIWNCIAARDATKADILVAEEPSADERLFC
>W.mirabilis.24347
MEAEHLGNKGSAPDVNDEGGGKVEEMVIWTSSGWCLSIKWNPVVGRYFIHATVRDRENAAKVQHLLHLPAANQRLRLFRADLSVEGSLDSAIDDCEGVFPIVSPVIIDPEDPKNEVIKVAVRGTLNILEACRRWMVPEHKMESCRRHLVKRLLELGYFIHATVRDRENAAKVQHLLHLPAANQRLRLFRADLSVEGSLDSAIDDCEGVFPIVSPVIIDPEDPKNEVIKVAVRGTLNILEACRREAVMDESSWSDVQFSRRKHPPGWAYILSKTEAEQTAFEYSKQNGLQVVGPCLTPSPPTSVELSVSPLTGNLGGIQQLNFLQRGYRWIPLVHVEDDCKANVFLFEAVACSSRYVCSTLTVSASEITLCLRQKYPNEYIDVDQWRRATVSSRKLFDLGFRYKRDDLGSILGEAVERIYSRGLFWRVFIGLIKHLVSYNPKKSKQNGLQVVGPCLTPSPPTSVELSVSPLTGNLGGIQQLNFLQRGYRWIPLVHVEDDCKANVFLFEAVACSSRYVCSTLTVSASEITLCLRQKYPNEYIDVDQWRRATVSSRKLFDLGFRYKRDDLGSILGEAVERIYSRGLFWV
>W.mirabilis.21375
MANSASANRRTEACVTGAGAFIMDRQMSPGRSCCVYGILRNPNDQKNSHLQTLNGATEHLKLFKADILHYHSLLQIIEGCDTVFRRRAPSGYFQIRKKKWETENRYIYSKTVAEQKAWKFCKEKGIHPVSLCPSLMLGPLLQKQLNSSSLILLKLLNGEMKKRENKIWNCIAVRDATKADILAAEEPYADERLFC
>W.mirabilis.22998
MDATAVFESKSKVCVTGASGYIASNLVSLLLRSGYSVNATVRDPENPSKVQHLLKLPGAESRLRLFKADLFEEGSFDAAIAGCEGVFHVATPSNFEPKDPENEVIKPAVKGTLDILESCRKSGTVKRVVYTSSAATLSWKREDQKHEYIDESSWSDLQFIRDSKPPGWPYVASKTEAERAALDFGREHGLCVITVVPVVVVGPWLTPSIGGSVAVAMSPLTGKAHCLYHLKGIQMSTGTVPLVHVDDVSEAHVFLYETPSASGRYVCCAQGITLSELGRLMRERYPQSYKGPTDFSDAEPTRELIVSSKKLMDAGFRFRHRDVASMIDDTVQCMRAKGYLS
MKLSRASVTLKTHRRRRSQERRILWEARCRRVFNILKSCHASRSVHKVLITSSATMLSCRPEFEKTGHVDGSSSSDMQSLRSEKPCRILLPKSRQRRPRMRTISSR
>W.mirabilis.20447
MRRIFMDPHKEMQKKATDEAQQSFGYLEDAQKTTFAGKAYFVGSPLSKGFFNILKSCHASRSVHKVLITSSATMLSCRPEFEKTGHVDGSSSSDMQSLRSEKPCRILLPKSRQRRPRMRTISSR
>W.mirabilis.00616
MDATAVSESKSKVCVTGASGYIASNLVSLLLRSGYSVNATVRDPENPSKVQHLLKLPGAQSRLHLFKADLFEEGSFDAAIAGCEGVFHVATPANFKPKDPENEVIKPAVKGTLDILESCRKSGTVKRVVYTSSVAALVWKQEDQKHEYINESSWSDLQFIRNSKPPAWPYVASKTEAERAALDFGREHGLHVITVVPVVVVGPWLTPSISASVALAMSPLTGKPHSLSNLKGFQMSTGTVPLIHVDDVSESHVFLYETPSASGRYVCCAQSITLSELGRLMRERYPQSYKGPTDFSDAEPTRELTVSSKKLIDAGFRFRHRDVASMIDDTVECMRAKGYLS
>W.mirabilis.08761
MQWRKEHAQQRLNINALPKSVRFAGVTDTRIAAFSILNSFIFLVYRISSQFSFLSIVYVDISQTWTLCAEASTKKPKVCVTGASGYVASALVKLLLRKGYSVHATVRDPENRSKVEHLLRLPGADAGLHLFRADLLEEGSFDAAVAGCEGVFHVASPLSFHPTDPEDDVIKPAVKGTLNILKSCRDSTSVRRVVITSSASTLPWKAEREKTVYVDESAWSDSQFLRSEKPAGWPYYVAKTEAEKAALEFGYENGLDVLTLVASVLVGPWLTSSMPASVGMVMSLLTPDPSSIGYLIAIQMGCGTVPLVHVDDICEAHVFIYEMSTCSGRYVCCASNIKLSELARHVCKKYPMICREAMDLRHVEAPFKIHISSRRLMEVGFQYRHTDVDSMLDDTVECMRGTLNILKSCRDSTSARRVVITSSASTLPWKAEREKTAYVDESAWSDSQFLRSEKPAGWPYFVAKTEAEKAALEFGYENGLDVLTLVAVLVVGPWLTSSIPASVGMVMSLLTPDPSSIGNLIAIQMGCGTVPLVHVDDICEAHVFIYEMSTCSGRYVCCASNIKLSELARHVCKKYPMICREAMDFRHVEAPFKIHISSRRLMDVGFQYRHTDVDSMLDDTVECMRGKGLLL
>W.mirabilis.08762
MDATAVSESKSRVCVTGASGYVASNLVSLLLRSGYCVNATVRDPVNPSKVQHLLKLPGAESRLRLFKADLFEEGSFDAAIAGCEGVFHLATPANFEPKDPENEVIQPTVKGTLDILESCRKSASVKRVVYTSSAATLTWKEENQKHEYIDESSWSDLQFIRNNKPPAWPYLVSKTEAERAALRFGREHGLHVITVVPVLVVGPWLTPSIPGSVAAAMSPLTGEPHFLYHLKGVQMSTGTVPLVHVDDVSETHVFVYETPSASGRHVCCAQGITLSELGRLMRERYPQSYKGPTDFSDAEPTRELTISSKKLMDAGFRFRRCQVASMIDDTVECMRAKGYLS
>W.mirabilis.08763
MDATAVSESKSKVCVTGASGYIASNLVSLLLRSGYSVNATVRDPENPSKVQHLLKLPGAKSRLHLFKADLFEEASFDAAIAGCEGVFHVATPSNFKPKDPENEVIKPAVKGTLDILESCRKSGTVKRVVYTSSAAALVWKQEDQKHEYINESSWSDLQFIRNSKPPAWPYVASKTEAERAALDFGRPVLGLSKPGPISS
>W.mirabilis.25660
MDAAATLESESKPTVCVTGVSGYIASSLVSLLLRLGYSIHGTVRNPENTSKTQHLMKLPGADKRLRIFKADLCEEGSYDAAVAGCQGLFHVAAPVDPNPNDPENEVIKVGIKGTLDVLESCRKTGSVKRVVLMSSFGALFWKEEDDGTNYLDELSSSDVDFLRRKKPPAWAYWIAKTESEKAALEFGRKNGMEVATLTPTLVVGRWITPSVPGSVLVATSLLTGHPGVAFLKAFNEACVRVELVHVDDVCAACVFVYETQSSIGRHVCCPVGIDLPALARRMRERYADSYVGPTDFDCMGPTGHVTFSCKKLTDMGFRFQYADVDSMMDDTAYWIAKTESEKAALEFGRKNGMEVATLTPPLVVGRWITPSVPGSVLVATSLLTGHPGVVFLKAFNEACVRVELVHVDDVCAACVFVYETQSSIGRHVCCPVGIDLPALARRMRERYADSYVGPTDFDCMGPTGHVTFSCKKLTDMGFRFQYADVDSMMDDTVKCMRVNGLLP
>W.mirabilis.09150
MQSASNYSFIGHLYSCSFVATLTWKKENQKHKYIDASSWSDFQFIRNNKPLARPYTVPKTEAEKESLHLGREHDLCIITVVPVLSVAAAMSPLTGKTHFLYDLEGVQISAGTMSLVHVDNIADR
>W.mirabilis.17816
MRVACVTGAGGFIASWIVKYLLDGGYSVHGTLRNPNDEKNSHLQALNGAKERLKLFKADVLAYESVLQAIDGCDTVFHTACPVPKTESWYLYAKTVSEQKAWEFCKEKGIHLVSLCPSLVLGPLLQKQLNFSSSILLKLVNGETEKCENTNRNCIDVRDVAKAHILAAEVPSAHGRYLCTCHFIDTKGMVDILRELYPQKTESWYLYAKTVSEQKAWEFCKEKGIHLVSLCPSLVLGPLLQKHLNFSSSILLKLVNGETEKCENTNRNCIDVRDVAKAHILAAEVPSAHGRYLCTCHFIDTKGMVDILRELYPQYDYPKEFVDTDQWSLKRASCSRLRQLENSTATTIKYYVVMKVV
>W.mirabilis.19214
MDATAISKSRSEVCMTDTSGHIASNLVSLLLCSEFSVNTTVHDPENPSKMQHLPNYLKRKIVFIFSKRISFRKGALMLQSPVVKESSMSSLHSTLSLKIPPKIFMAYLSITFTEDALQKKKSSTLLKVSQNGGLRR
>W.mirabilis.19215
MDATAISKSRSEVCMTDTSGYIASNLVSLLLCSEFSVNTTVHDPENPSKMQHLPNYLKRKIVFIFSKRISFRKGALMLQSPVVKESSMSSLHSTLSLKIPPKIFMAYLSITFTEDALQKKNPPRY
>W.mirabilis.19815
MLGPLLQKQLNSSSLILLKLLNGEMKKRENKIWNCIAIIEGCDTVFQTACPVGIFSNPEEEMVKPSVEGTLNVLKACSLSKVRRVIFTSSLTAIRFTPKIPGDKVLMKVAGRIWNIAGRLRIGTFTRRQWQSKRHGSFKQLNSSSLILLKLLNGEMKKRENKIWNCIAARDATKADILVAEEPSADERLFC
>W.mirabilis.24347
MEAEHLGNKGSAPDVNDEGGGKVEEMVIWTSSGWCLSIKWNPVVGRYFIHATVRDRENAAKVQHLLHLPAANQRLRLFRADLSVEGSLDSAIDDCEGVFPIVSPVIIDPEDPKNEVIKVAVRGTLNILEACRRWMVPEHKMESCRRHLVKRLLELGYFIHATVRDRENAAKVQHLLHLPAANQRLRLFRADLSVEGSLDSAIDDCEGVFPIVSPVIIDPEDPKNEVIKVAVRGTLNILEACRREAVMDESSWSDVQFSRRKHPPGWAYILSKTEAEQTAFEYSKQNGLQVVGPCLTPSPPTSVELSVSPLTGNLGGIQQLNFLQRGYRWIPLVHVEDDCKANVFLFEAVACSSRYVCSTLTVSASEITLCLRQKYPNEYIDVDQWRRATVSSRKLFDLGFRYKRDDLGSILGEAVERIYSRGLFWRVFIGLIKHLVSYNPKKSKQNGLQVVGPCLTPSPPTSVELSVSPLTGNLGGIQQLNFLQRGYRWIPLVHVEDDCKANVFLFEAVACSSRYVCSTLTVSASEITLCLRQKYPNEYIDVDQWRRATVSSRKLFDLGFRYKRDDLGSILGEAVERIYSRGLFWV
>W.mirabilis.21375
MANSASANRRTEACVTGAGAFIMDRQMSPGRSCCVYGILRNPNDQKNSHLQTLNGATEHLKLFKADILHYHSLLQIIEGCDTVFRRRAPSGYFQIRKKKWETENRYIYSKTVAEQKAWKFCKEKGIHPVSLCPSLMLGPLLQKQLNSSSLILLKLLNGEMKKRENKIWNCIAVRDATKADILAAEEPYADERLFC
>W.mirabilis.22998
MDATAVFESKSKVCVTGASGYIASNLVSLLLRSGYSVNATVRDPENPSKVQHLLKLPGAESRLRLFKADLFEEGSFDAAIAGCEGVFHVATPSNFEPKDPENEVIKPAVKGTLDILESCRKSGTVKRVVYTSSAATLSWKREDQKHEYIDESSWSDLQFIRDSKPPGWPYVASKTEAERAALDFGREHGLCVITVVPVVVVGPWLTPSIGGSVAVAMSPLTGKAHCLYHLKGIQMSTGTVPLVHVDDVSEAHVFLYETPSASGRYVCCAQGITLSELGRLMRERYPQSYKGPTDFSDAEPTRELIVSSKKLMDAGFRFRHRDVASMIDDTVQCMRAKGYLS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University