Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Vitis vinifera
Species name
Wine grape (Vitis vinifera) is a species of eudicot in the family Vitaceae (grape family).
Species info
Gene sequence info
Vitis_vinifera.ANAT.fa
Download
>rna-XM_019223275.1
MNFPIPKLILWRPSFLASNTRFLLPSSIFFPLSGNLIFPLDCSIPEIRYKDGDSVSLTFVEGSFDFDHLSGNHQRNDIDFHPLAVQMSPASTTFGPLIVPLLAIQVTLFPNSGICIGFTFHHIAGDANTFVRFARSWASINKLGGDATSVESLILPFYDRTVVKDPIGLRSTFWNYFGKVKFEGCQSQLSTNNVRAKFLLRRAEVQRLKKWVIPQIPKQSHVSAFTVICAYVWSCMIKARSRSGEDVGENELEHFAFTADCRTLLDPPIPAAYFGNCLMGGLATTKSTRLIQEDGFIVAAKSIREAIHERVRNKGGVLKGVQQWVSDLKSLNRMRMAAVVGSPRFQVYNVDFGFGRPKKYEVISRDRYFTLDGCKDNEEDFEIGLCFPKAKMDAFAEIFTNGLEAYSCL
>rna-XM_002276054.3
MASFEMVNVLEHCRLAPAPGTVDEKSLPLTFFDIPWLHFPLIQHLFFYEFPYPKSHFIDTIIPLLKHSLSLALKHFYPFAGKLIFPPNFGKPEIRYVSGDAVSLVFAESNKDFNHITGNHQRNVAEFHSLVAQLPPVSIISDSLVAPLLAVQVTLFPNSGICIGFTFRRVTADGNACTRFIRSWASINKVGRETTMVESGTLPFYDRTMVKDPNGLASVFWNHVGKIKFEGSQRQLSTNKVIATFILGQSHVQQLKKWVLSQCPTSSHVSTFTVTCAYVWTCMVKARERSGEKVTEDELEHFVFGADCRALLQPPLPATYFGNCLVSCFATTKTKELMGDNGFIIAAKTIGEAIQERLRKKEGVLKDAEKWISEFETRKEDRIVGVAGSPRFAVYDIDFGLGKPKKSEVVSIDMTGSISLNECKNDKESFEIGLSLPKIKMDAFEDIFANGFQV
>rna-XM_010659084.2
MASSDEMVNVLEECRVSPPPNAVGEKSLPLTFFDLLWLHFHLVQSLFFYKFPHSKTHFIEITIPSLKHSLSLALKHFYPFAGNLLFPPNLREPEIHYVDGDSVSLTFVESNSDFDYLIKNHQRKIAHFHPLVPQLPPVSVQQGTIIAPVFAVQVTLFPNSGISFGFTCDHVVADGNAFIRFIRLWATIHKLQREPLMLEGEILPFYDRTMVKDPNKIGSIFWQHHQKSKFEGYRPLLPTSNILASFLLSQADLQRLKKRVMVQCPTLLHVSSFTVTCAYTWTCIVKAQVWSGEEVSENELEHFGFVADCRACLDPPLPENYFANLIGEAIQEKLGSKKGVLEGLDKWVVNFSSINIERAVGVAGSPRFSVYDIDFGLGQLQKREFISIDETRSISLLEGKDNKSDIEVGLSFPKIKMDAFASIFTNGLSVYD
>rna-XM_019223377.1
MASPEMVHVLEHCRIAPPPGSVDGKSLPLTFFDLPWLHFPLMNFFFFYEFSHPKTYFVETIIPRLKHSLSLTLKHFFPLSGNLIFPLDCSIPEIRYKDGDSVSLTFVEGSFDFDHLSGHHQRNEIDFHPLAVQMSPASTSTFGPLIVPLLAIQVTLFPNSGICIGFTFHHIAGNANTFFRLARSWASINKLGGDATLVESLILPFYDRTVVKDPIGLRSTFWNYFGKVKFEECQSQLSTNSVRATFLLRRAEVQRLKKWVIPQIPKQSHVSAFTVVCAYVWSCMIKARSRSGEDVGENELEHFAFTADCRTLLDPPIPAAYFGNCLMGGLATTKSIAANSIREAIQERVRNKGEVLKGVQKWVSDLKSLNRMRMAAVVGSPRFEVYNMDFGLGRPKKYEVISRDRYFTLDGCKDNEEDLRLGCASPRLKWMPLRRSLPMASRLIPVYNNKDNDIVRKNKMKIVNDIVTISIVSNFSLIMWYE
>rna-XM_002275877.3
MASTEMVTVLEECRVSPPSSTAVDEKSLPLTFFDIPWIHQHLVQSVFFYEYPHPKTHFIETTIPALKHSLSLTLKHFYPFAGNLLFPPNLGKPQIHYADGDSVSLTFVESSDDFSYLTANHQRDVARLHPLIPQMPPVSVSNDTLVAPLVAIQVTLFPNSGISFGFTTNHVVADGNTFIRFVRLWASINKLGEESMTLEGAILPFYDRTMVKDSNGVGSIFWQHMGKKKFGGYQPQLPTNNVLGTFLLSQAHLQRLKRWVMVQCPKLPHVSSLTVACSYTWACVAKARARSSDEVDENELQHFVFPVDCRARLNPPLPENYFGNCLAPCFVAIKSTQLIDDEDGFVAAVNAMGEALGKTLQNEEGVLRGAEKWLSDLESLNKERTLGASASPKFAVYNIDFGFGRLQKREMISIDETNSISLHEGKTNMGDIEVGVALPKMTMDVFASIFANGLKVYD
>rna-XM_010659095.1
MASFETVNVLERCQVTPPPVMVDEKSKALPLTFFDMPWLFPDDGKDFNYLIGNHPRNVADFHPLVPQLSPVFMSSSILVAPLLAIQVTLLPNFGISLGFTFPYSVADDNTCSQFNVPSLTSHHKKEYRKVVSSSIALEEPYFFNTTAKWIQLSTRPSTNNVLDTFVLNQEDLQRLKRWVAAKCPTLSHVSSFTVACAYVWDCMGKARAKSGEDVGENEPEHLAFVGGCRAYFDPPIPANYFGNCLAPCSATVKSLELITEDGFIVATNTIGKAIQERLRNKEGILKGLEKWMSNYKSLNPKRIFGVAGLPKFSCYDIDFGLGRPCKGEIILIDVTKSISLNEGKNNKEDVEIDLSFPKIKMDVFTSIFVNGLKIYD
>rna-XM_010659091.1
MASFETVNVLERCQVAPPPVMVDEKSKSLPLTFFDMPWLHSHLMESLIFYAFPHPKTHFIEAIIPNFKHSLSLALRHFYFLAGNLIFPPNFTKPEIRYKDGDSVSLIFAESTRDFNYLIGNHPRNVAEFHPLVPQLSPVSMSSSILAAPLLAIQVTLFPNFGISLGFTFPHSVADGNTFSQFVRLWASINKLGEETAMLQDRILPFYDRTVVKDPLGIESIFWNQVGKIKFEGYRPRPPTNNVLATFVLSQEDLQRLKRWVAAKCPTLSHVSSFTVACAYVWACMAKARAKSGEDVGENEPEHLAFVGDCRAYFDPPIPANYFGNCLAPCSATVKSLELITEDGFIVAANTIGKAIQERLRNKEGILKGLEKWMSNYKSLNPKRIFGVAGSPKFSCYDIDFGLGRPCKGEIISIDVTKSISLNEGRNNKEDVEIGLSFPKIKMDVFTSIFVNGLKIYD
>rna-XM_003633529.3
MAPPIAVRVLDRYRVAPPPGSAPPTSLPLTFFDIPWLLFPPSQPLFFFQFPIPTSHFTSVTLPKLKHSLSLTLLEYFPFSGRLSHYPESETPAIVYAEGDSVGMTVAEADGDFDNFCGHHSKDACEFHQLVPRLSESDEVPMMAVQITVFSNAGISIGLALHHAVSDGRTFTNFMKTWSSIYRNGEKSLPKSFPLPFYDRSVIKELKGLKEIFLQQWRKHKKYLSKTSREDREDDSVRATFVMSPREMEKVKKWIMAHCRRKGKPPPPLLSAYVLTCAFVWVCLCEARAGRVSTAPPDTCFFGFNAGGITRLDYPVPVAYLGNCVAFGRAVAGRSDLMGEEGIVVAAEAIGRKIKELDGEVLGGAERWILEWEVLIGSEVHVVVLGSPKLDLYEVDFGWGRPRKIEEVSIDEMKSGISLTESRDIKGGIEIGLVLPNDQMDVFASLFHGGLKVLP
MNFPIPKLILWRPSFLASNTRFLLPSSIFFPLSGNLIFPLDCSIPEIRYKDGDSVSLTFVEGSFDFDHLSGNHQRNDIDFHPLAVQMSPASTTFGPLIVPLLAIQVTLFPNSGICIGFTFHHIAGDANTFVRFARSWASINKLGGDATSVESLILPFYDRTVVKDPIGLRSTFWNYFGKVKFEGCQSQLSTNNVRAKFLLRRAEVQRLKKWVIPQIPKQSHVSAFTVICAYVWSCMIKARSRSGEDVGENELEHFAFTADCRTLLDPPIPAAYFGNCLMGGLATTKSTRLIQEDGFIVAAKSIREAIHERVRNKGGVLKGVQQWVSDLKSLNRMRMAAVVGSPRFQVYNVDFGFGRPKKYEVISRDRYFTLDGCKDNEEDFEIGLCFPKAKMDAFAEIFTNGLEAYSCL
>rna-XM_002276054.3
MASFEMVNVLEHCRLAPAPGTVDEKSLPLTFFDIPWLHFPLIQHLFFYEFPYPKSHFIDTIIPLLKHSLSLALKHFYPFAGKLIFPPNFGKPEIRYVSGDAVSLVFAESNKDFNHITGNHQRNVAEFHSLVAQLPPVSIISDSLVAPLLAVQVTLFPNSGICIGFTFRRVTADGNACTRFIRSWASINKVGRETTMVESGTLPFYDRTMVKDPNGLASVFWNHVGKIKFEGSQRQLSTNKVIATFILGQSHVQQLKKWVLSQCPTSSHVSTFTVTCAYVWTCMVKARERSGEKVTEDELEHFVFGADCRALLQPPLPATYFGNCLVSCFATTKTKELMGDNGFIIAAKTIGEAIQERLRKKEGVLKDAEKWISEFETRKEDRIVGVAGSPRFAVYDIDFGLGKPKKSEVVSIDMTGSISLNECKNDKESFEIGLSLPKIKMDAFEDIFANGFQV
>rna-XM_010659084.2
MASSDEMVNVLEECRVSPPPNAVGEKSLPLTFFDLLWLHFHLVQSLFFYKFPHSKTHFIEITIPSLKHSLSLALKHFYPFAGNLLFPPNLREPEIHYVDGDSVSLTFVESNSDFDYLIKNHQRKIAHFHPLVPQLPPVSVQQGTIIAPVFAVQVTLFPNSGISFGFTCDHVVADGNAFIRFIRLWATIHKLQREPLMLEGEILPFYDRTMVKDPNKIGSIFWQHHQKSKFEGYRPLLPTSNILASFLLSQADLQRLKKRVMVQCPTLLHVSSFTVTCAYTWTCIVKAQVWSGEEVSENELEHFGFVADCRACLDPPLPENYFANLIGEAIQEKLGSKKGVLEGLDKWVVNFSSINIERAVGVAGSPRFSVYDIDFGLGQLQKREFISIDETRSISLLEGKDNKSDIEVGLSFPKIKMDAFASIFTNGLSVYD
>rna-XM_019223377.1
MASPEMVHVLEHCRIAPPPGSVDGKSLPLTFFDLPWLHFPLMNFFFFYEFSHPKTYFVETIIPRLKHSLSLTLKHFFPLSGNLIFPLDCSIPEIRYKDGDSVSLTFVEGSFDFDHLSGHHQRNEIDFHPLAVQMSPASTSTFGPLIVPLLAIQVTLFPNSGICIGFTFHHIAGNANTFFRLARSWASINKLGGDATLVESLILPFYDRTVVKDPIGLRSTFWNYFGKVKFEECQSQLSTNSVRATFLLRRAEVQRLKKWVIPQIPKQSHVSAFTVVCAYVWSCMIKARSRSGEDVGENELEHFAFTADCRTLLDPPIPAAYFGNCLMGGLATTKSIAANSIREAIQERVRNKGEVLKGVQKWVSDLKSLNRMRMAAVVGSPRFEVYNMDFGLGRPKKYEVISRDRYFTLDGCKDNEEDLRLGCASPRLKWMPLRRSLPMASRLIPVYNNKDNDIVRKNKMKIVNDIVTISIVSNFSLIMWYE
>rna-XM_002275877.3
MASTEMVTVLEECRVSPPSSTAVDEKSLPLTFFDIPWIHQHLVQSVFFYEYPHPKTHFIETTIPALKHSLSLTLKHFYPFAGNLLFPPNLGKPQIHYADGDSVSLTFVESSDDFSYLTANHQRDVARLHPLIPQMPPVSVSNDTLVAPLVAIQVTLFPNSGISFGFTTNHVVADGNTFIRFVRLWASINKLGEESMTLEGAILPFYDRTMVKDSNGVGSIFWQHMGKKKFGGYQPQLPTNNVLGTFLLSQAHLQRLKRWVMVQCPKLPHVSSLTVACSYTWACVAKARARSSDEVDENELQHFVFPVDCRARLNPPLPENYFGNCLAPCFVAIKSTQLIDDEDGFVAAVNAMGEALGKTLQNEEGVLRGAEKWLSDLESLNKERTLGASASPKFAVYNIDFGFGRLQKREMISIDETNSISLHEGKTNMGDIEVGVALPKMTMDVFASIFANGLKVYD
>rna-XM_010659095.1
MASFETVNVLERCQVTPPPVMVDEKSKALPLTFFDMPWLFPDDGKDFNYLIGNHPRNVADFHPLVPQLSPVFMSSSILVAPLLAIQVTLLPNFGISLGFTFPYSVADDNTCSQFNVPSLTSHHKKEYRKVVSSSIALEEPYFFNTTAKWIQLSTRPSTNNVLDTFVLNQEDLQRLKRWVAAKCPTLSHVSSFTVACAYVWDCMGKARAKSGEDVGENEPEHLAFVGGCRAYFDPPIPANYFGNCLAPCSATVKSLELITEDGFIVATNTIGKAIQERLRNKEGILKGLEKWMSNYKSLNPKRIFGVAGLPKFSCYDIDFGLGRPCKGEIILIDVTKSISLNEGKNNKEDVEIDLSFPKIKMDVFTSIFVNGLKIYD
>rna-XM_010659091.1
MASFETVNVLERCQVAPPPVMVDEKSKSLPLTFFDMPWLHSHLMESLIFYAFPHPKTHFIEAIIPNFKHSLSLALRHFYFLAGNLIFPPNFTKPEIRYKDGDSVSLIFAESTRDFNYLIGNHPRNVAEFHPLVPQLSPVSMSSSILAAPLLAIQVTLFPNFGISLGFTFPHSVADGNTFSQFVRLWASINKLGEETAMLQDRILPFYDRTVVKDPLGIESIFWNQVGKIKFEGYRPRPPTNNVLATFVLSQEDLQRLKRWVAAKCPTLSHVSSFTVACAYVWACMAKARAKSGEDVGENEPEHLAFVGDCRAYFDPPIPANYFGNCLAPCSATVKSLELITEDGFIVAANTIGKAIQERLRNKEGILKGLEKWMSNYKSLNPKRIFGVAGSPKFSCYDIDFGLGRPCKGEIISIDVTKSISLNEGRNNKEDVEIGLSFPKIKMDVFTSIFVNGLKIYD
>rna-XM_003633529.3
MAPPIAVRVLDRYRVAPPPGSAPPTSLPLTFFDIPWLLFPPSQPLFFFQFPIPTSHFTSVTLPKLKHSLSLTLLEYFPFSGRLSHYPESETPAIVYAEGDSVGMTVAEADGDFDNFCGHHSKDACEFHQLVPRLSESDEVPMMAVQITVFSNAGISIGLALHHAVSDGRTFTNFMKTWSSIYRNGEKSLPKSFPLPFYDRSVIKELKGLKEIFLQQWRKHKKYLSKTSREDREDDSVRATFVMSPREMEKVKKWIMAHCRRKGKPPPPLLSAYVLTCAFVWVCLCEARAGRVSTAPPDTCFFGFNAGGITRLDYPVPVAYLGNCVAFGRAVAGRSDLMGEEGIVVAAEAIGRKIKELDGEVLGGAERWILEWEVLIGSEVHVVVLGSPKLDLYEVDFGWGRPRKIEEVSIDEMKSGISLTESRDIKGGIEIGLVLPNDQMDVFASLFHGGLKVLP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University