Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Vernicia fordii
Species name
Tung oil tree (Vernicia fordii) is a species of eudicot in the family Euphorbiaceae (spurge family).
Species info
Gene sequence info
Vernicia_fordii.ANR.fa
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>tung.mrna.scaffold1192.00004.001
MSGEGRVVCVTGGAGYIASWLVKLLLQRGYTVRATVRDPNDPKKTEHLLALDGAKERLHLFKANLVEEGCFDSIVDGCEGVFHTASPVILSTNEPQRVVITSSMAAVMFNGKPLSPDVLWYAVSKTLAEDAAWKFAKENGIDVVTINPGFVIGPLLKPTLNLTVEVILKHVNGAQTFPNDNYRFVDVRDIANAHIQAFERRSASGRYCLVATVAHFSEVLKIVRDHYPTLCLPEKQVTDIFCYGGCHSNWSGLIEQ
>tung.mrna.scaffold1617.00007.001
MKSETVCVTGASGFIGSWLVMRLLERGYYVKATVRDPGNMNKVKHLLELANASTHLSLWKADLVEEGSFDDAIEGCTCVFHVATPMEFLKFQDPEVIACTPIKCLIIVHDDSISSYNMLWQNEIIKPTVNGVLHVMRSCLKAKSIKRFIYTSSSGTIVVKPPPPPPEYDESCWTDIDFCYAQKMLGWPYNVAKTMAEKAAWEFAKQNGIHLITIHPSLVIGPFLTPSRPISVEIGLSLITGNETFYQCSIFLFENPQANGRYICSNHDFTILDLAKSLSQKYPEYNVPTKFEGVDESLKVIPWSPKKLMDLGFTFKYDSGKYDVGDLCAEAIELCKEKGLL
>tung.mrna.scaffold26.00143.001
MSEAAVAGVKSIVESCIKAGTVKRLIYTASVVASSPMEEDGIGFKHTMDESCWTPINNLSFAQNENALMAYVRSKVLSEQEVIRHNSNELEVVSITCGLVGGDTIRSGLSQSMEILISQVTKDKQWYHTLRFLEDLMGKVPIVHIEDVRDVHIFCIEKSSITGRFLCATKCFSSAEIANYWQNNYPNIVINEEFVENLGRDIVLNSTKFKEIGFEYKHGAEKILDDTLKCAQELSKFSFNSN
>tung.mrna.scaffold383.00026.001
MHRWSGVPIPISPLLSKITRKKIKTDMEDCVTKVCVTGGSGYVGSLLVKKLLEKGYIVHATLRNLEDKSKVDPLKSFPIAETNLFLFQADIYNSTEFEPAIQGCQFVFHVATPMLHDPKSSQYKNKVEATVAGAKSIVESCIKSKTVKRLIYTASVLSTSPLNEDGKNFKSIMDESCWTPLHLSFSFANDFVMEYTKGKTLAEKLVLSYNENEDDKLEVVTLACALIGGETILSHEIESIKVIYSPITGDLFGYNSLRFLQEVLGTIPIVHVDDVCEAHIFCMEKPSLKGRFLCSVANPTSSRDIAVYYTENYPQFKIDEKFMGEAAEREIKLDSSKLTSMGFVYKYNLKNILDESLECGRRLGAICR
>tung.mrna.scaffold383.00025.001
MEYCYTKVCVTGGSGYIGSWLVKKLLEKGYTVHATLRNLEDKSKVDFLKSLPKANTNLVLFQADIYNSSEFEAAIQGCEFVFHVASPMANHDHKSSQYKDKIQVTVAGVKSIVDCCIKSKTVKRLIYTASVLAASPLNEDGNGFKSSMDESCWTSPHLSFSFVDHRAMEYAIGKMLAEKEVLRYNKIEDAKVEVVTLVCGLVGGETILSHAPISVKAIYSQITGDLFGYNYALRFMQEVLGTIPIVHVDDVCEAHIFCMEKPSLKGRFLCSVANPTSTRDIAVYYTENYPQFKIDDQFMGEPAGREIILDSSKLTNMGFAYKYDLKNILDENLEWGRRLGDIIT
>tung.mrna.scaffold471.00017.001
MSGEGKVVCVSGGSGYVASWIVKLLLQHGYTVKTTVRDPNDPKKTEHLHALDGAKERLHLFKANLVEEGSFDPLIDGCDGVFHTASPVILSSNELQVELLEPAIKGTLSILKSCAKVPSIKRVVLTSSIAAIIFNGKPLIPDMYWYPLSKTLAENAAWKFAKENGIDSVTINPGWVIGPLLQPTTLNLSMEVILNHVNGAETFPNDIFRLVDVRDVANAHIQAFERPSASGRYCLVGKVVHFSEPFVSKHEMSKEKAKSLGIDFTPLEITLKDTIESLKEKGFINL
MSGEGRVVCVTGGAGYIASWLVKLLLQRGYTVRATVRDPNDPKKTEHLLALDGAKERLHLFKANLVEEGCFDSIVDGCEGVFHTASPVILSTNEPQRVVITSSMAAVMFNGKPLSPDVLWYAVSKTLAEDAAWKFAKENGIDVVTINPGFVIGPLLKPTLNLTVEVILKHVNGAQTFPNDNYRFVDVRDIANAHIQAFERRSASGRYCLVATVAHFSEVLKIVRDHYPTLCLPEKQVTDIFCYGGCHSNWSGLIEQ
>tung.mrna.scaffold1617.00007.001
MKSETVCVTGASGFIGSWLVMRLLERGYYVKATVRDPGNMNKVKHLLELANASTHLSLWKADLVEEGSFDDAIEGCTCVFHVATPMEFLKFQDPEVIACTPIKCLIIVHDDSISSYNMLWQNEIIKPTVNGVLHVMRSCLKAKSIKRFIYTSSSGTIVVKPPPPPPEYDESCWTDIDFCYAQKMLGWPYNVAKTMAEKAAWEFAKQNGIHLITIHPSLVIGPFLTPSRPISVEIGLSLITGNETFYQCSIFLFENPQANGRYICSNHDFTILDLAKSLSQKYPEYNVPTKFEGVDESLKVIPWSPKKLMDLGFTFKYDSGKYDVGDLCAEAIELCKEKGLL
>tung.mrna.scaffold26.00143.001
MSEAAVAGVKSIVESCIKAGTVKRLIYTASVVASSPMEEDGIGFKHTMDESCWTPINNLSFAQNENALMAYVRSKVLSEQEVIRHNSNELEVVSITCGLVGGDTIRSGLSQSMEILISQVTKDKQWYHTLRFLEDLMGKVPIVHIEDVRDVHIFCIEKSSITGRFLCATKCFSSAEIANYWQNNYPNIVINEEFVENLGRDIVLNSTKFKEIGFEYKHGAEKILDDTLKCAQELSKFSFNSN
>tung.mrna.scaffold383.00026.001
MHRWSGVPIPISPLLSKITRKKIKTDMEDCVTKVCVTGGSGYVGSLLVKKLLEKGYIVHATLRNLEDKSKVDPLKSFPIAETNLFLFQADIYNSTEFEPAIQGCQFVFHVATPMLHDPKSSQYKNKVEATVAGAKSIVESCIKSKTVKRLIYTASVLSTSPLNEDGKNFKSIMDESCWTPLHLSFSFANDFVMEYTKGKTLAEKLVLSYNENEDDKLEVVTLACALIGGETILSHEIESIKVIYSPITGDLFGYNSLRFLQEVLGTIPIVHVDDVCEAHIFCMEKPSLKGRFLCSVANPTSSRDIAVYYTENYPQFKIDEKFMGEAAEREIKLDSSKLTSMGFVYKYNLKNILDESLECGRRLGAICR
>tung.mrna.scaffold383.00025.001
MEYCYTKVCVTGGSGYIGSWLVKKLLEKGYTVHATLRNLEDKSKVDFLKSLPKANTNLVLFQADIYNSSEFEAAIQGCEFVFHVASPMANHDHKSSQYKDKIQVTVAGVKSIVDCCIKSKTVKRLIYTASVLAASPLNEDGNGFKSSMDESCWTSPHLSFSFVDHRAMEYAIGKMLAEKEVLRYNKIEDAKVEVVTLVCGLVGGETILSHAPISVKAIYSQITGDLFGYNYALRFMQEVLGTIPIVHVDDVCEAHIFCMEKPSLKGRFLCSVANPTSTRDIAVYYTENYPQFKIDDQFMGEPAGREIILDSSKLTNMGFAYKYDLKNILDENLEWGRRLGDIIT
>tung.mrna.scaffold471.00017.001
MSGEGKVVCVSGGSGYVASWIVKLLLQHGYTVKTTVRDPNDPKKTEHLHALDGAKERLHLFKANLVEEGSFDPLIDGCDGVFHTASPVILSSNELQVELLEPAIKGTLSILKSCAKVPSIKRVVLTSSIAAIIFNGKPLIPDMYWYPLSKTLAENAAWKFAKENGIDSVTINPGWVIGPLLQPTTLNLSMEVILNHVNGAETFPNDIFRLVDVRDVANAHIQAFERPSASGRYCLVGKVVHFSEPFVSKHEMSKEKAKSLGIDFTPLEITLKDTIESLKEKGFINL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University