Enzyme
This enzyme is a member of the aromatic amino acid lyase family, other members of which are EC 4.3.1.3 (histidine ammonia-lyase) and EC 4.3.1.23 (tyrosine ammonia-lyase) and EC 4.3.1.25 (phenylalanine/tyrosine ammonia-lyase). The enzyme contains the cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO), which is common to this family [3]. This unique cofactor is formed autocatalytically by cyclization and dehydration of the three amino-acid residues alanine, serine and glycine [9]. The enzyme from some species is highly specific for phenylalanine [7,8].
PAL
Enzyme
Phenylalanine ammonia-lyase
Enzyme name
EC:4.3.1.24
Entry
Lyases;Carbon-nitrogen lyases;Ammonia-lyases
Class
L-phenylalanine ammonia-lyase (trans-cinnamate-forming)
Sysname
L-phenylalanine = trans-cinnamate + NH3[RN:R00697]
Reaction
trans-cinnamate [CPD:C00423];NH3 [CPD:C00014]
Product
Species information
Vernicia fordii
Species name
Tung oil tree (Vernicia fordii) is a species of eudicot in the family Euphorbiaceae (spurge family).
Species info
Gene sequence info
Vernicia_fordii.PAL.fa
Download
>tung.mrna.scaffold11448.00001.001
MDVESDHILHGGNFQAAVVTSTMDRTRLCLERLGKMLFSQATEIINPMLNGGLPANLCAD
>tung.mrna.scaffold11448.00002.001
MGSLSKQGIPTTNARPLISSSVLAWKAFQKSLQDEHCIIDGNSLTIASVVGVANTKALVCLRVKILAALKMLQRQTILFTCEISIWSPAQTACQRXSWVRASMLIRCNSIIRGHSGVRICVAQTILHLLNKNIIPVVPLRGSISASGDLSPLSYIAGAIEGSPDIYVRLADGRVLSAAEALSDAGIEPVIFQPKEGLGLLNGTAFSAATASLALHQSRDIVLLSQVLTAMSVEAMQGSAEPFWPQIARMRPHPGXRFAKEYKDDWSLPRPLCLENCISVGRTSNRGPFTRHFSDPN
>tung.mrna.scaffold13981.00001.001
FLNAGIFGNGTETCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPTGESLDAVEAFRLAGIDSGFFELQPKEGLALVNGTAVGSGLASMVLFDANVLAILSEILSAIFAEVMNGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYIKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPQIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNARLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTTHVQSAEQHNQDVNSLGLISSRKTQEAIDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNT
>tung.mrna.scaffold1775.00019.001
MATNSISPSIEAIPLCNSFVSTPSPHWKMAADSLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAIARRSEVGVQLNELAARDRVAKSANWVAVTISRGTDTYGVTTGFGATSHRRTNKTGDLQTELIRFLNAGVIGKENLPTSYSKAAMLVRTNTLMQGYSGIRWEILESIAKLMNQNLIPKLPLRGTITASGDLVPLSYIAGLLTGRHNSKVVTLQGQEITAIEALKRAGIQAPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAEILSALFCEVMHGKPEYTDPLTHQLKHHPGQIEAAAIMKHLLDESDYMKEAKIRHEKDPLTKPKQDRYAIRTSPQWLGPQIEVIRAATHSIEREINSVNDNPLIDVARDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATFVVAICQAIDLRHLEENMREVVKQIVRQVIRKTLYMAEDGSLLESRFCEKELLQVVEHQPVFSYLDDPTNPSYALLPKLQEVLVQKALKNPESENSDKKGYTIFKRIPVFMEELKRRLEEEVPKARERFDSGEFGIENRIKKCRTYPIYEFVRSEVGTELLSGAKKVSPGEDIEKVHEAINEGKLGEVLIKCLANWKETCPSV
>tung.mrna.scaffold1775.00007.001
MATNSISPSIEAIPLCNSFVSTPSPHWKMAADSLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAIARRSEVGVQLNELAARDRVAKSANWVAVTISRGTDTYGVTTGFGATSHRRTNKTGDLQTELIRFLNAGVIGKENLPTSYSKAAMLVRTNTLMQGYSVIRKTLYMAEDGSLLESRFCEKELLQVVEHQPVFSYLDDPTNPSYALLPKLQEVLVQKALKNPESENSDKKGYTIFKRIPVFMEELKRRLEEEVPKARERFDSGEFGIENRIKKCRTYPIYEFVRSEVGTELLSGAKKVSPGEDIEKVHEAINEGKLGEVLIKCLANWKETCPSV
>tung.mrna.scaffold245.00102.001
MATNSISPSIEAIPLCNSFVSTPSPHWKMAADSLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAIARRSEVGVQLNELAARDRVAKSANWVAVTISRGTDTYGVTTGFGATSHRRTNKTGDLQTELIRFLNAGVIGKENLPTSYSKAAMLVRTNTLMQGYSGIRWEILESIAKLMNQNLIPKLPLRGTITASGDLVPLSYIAGLLTGRHNSKVATLQGQEITAIEALKRAGIQAPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAEILSALFCEVMHGKPEYTDPLTHQLKHHPGQIEAAAIMKHLLDESDYMKEAKIRHEKDPLTKPKQDRYAIRTSPQWLGPQIEVIRAATHSIEREINSVNDNPLIDVARDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATFVVAICQAIDLRHLEENMREVVKQIVRQVIRKTLYMAEDGSLLESRFCEKELLQVVEHQPVFSYLDDPTNPSYALLPKLQEVLVQKALKDPKSENSDKNGYTIFKRIPVFMEELKRRLEEEVPKARERFDSGEFGIENRIKKCRTYPIYEFVRSEVGTELLSGAKKVSPGEDIEKVHEAINEGKLGEVLIKCLANWKETCPSV
>tung.mrna.scaffold245.00101.001
MATNSISSSIEAIPLCNSFVSTPSPHWKMAAESLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAVARRSEVSVQLNELAARDRVAKSANWVAVNISRGTDTYXHRSIKYLQQNNSPTTMRQLFS
MDVESDHILHGGNFQAAVVTSTMDRTRLCLERLGKMLFSQATEIINPMLNGGLPANLCAD
>tung.mrna.scaffold11448.00002.001
MGSLSKQGIPTTNARPLISSSVLAWKAFQKSLQDEHCIIDGNSLTIASVVGVANTKALVCLRVKILAALKMLQRQTILFTCEISIWSPAQTACQRXSWVRASMLIRCNSIIRGHSGVRICVAQTILHLLNKNIIPVVPLRGSISASGDLSPLSYIAGAIEGSPDIYVRLADGRVLSAAEALSDAGIEPVIFQPKEGLGLLNGTAFSAATASLALHQSRDIVLLSQVLTAMSVEAMQGSAEPFWPQIARMRPHPGXRFAKEYKDDWSLPRPLCLENCISVGRTSNRGPFTRHFSDPN
>tung.mrna.scaffold13981.00001.001
FLNAGIFGNGTETCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPTGESLDAVEAFRLAGIDSGFFELQPKEGLALVNGTAVGSGLASMVLFDANVLAILSEILSAIFAEVMNGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYIKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPQIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNARLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTTHVQSAEQHNQDVNSLGLISSRKTQEAIDILKLMSSTFLVALCQAIDLRHLEENLKHTVKNT
>tung.mrna.scaffold1775.00019.001
MATNSISPSIEAIPLCNSFVSTPSPHWKMAADSLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAIARRSEVGVQLNELAARDRVAKSANWVAVTISRGTDTYGVTTGFGATSHRRTNKTGDLQTELIRFLNAGVIGKENLPTSYSKAAMLVRTNTLMQGYSGIRWEILESIAKLMNQNLIPKLPLRGTITASGDLVPLSYIAGLLTGRHNSKVVTLQGQEITAIEALKRAGIQAPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAEILSALFCEVMHGKPEYTDPLTHQLKHHPGQIEAAAIMKHLLDESDYMKEAKIRHEKDPLTKPKQDRYAIRTSPQWLGPQIEVIRAATHSIEREINSVNDNPLIDVARDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATFVVAICQAIDLRHLEENMREVVKQIVRQVIRKTLYMAEDGSLLESRFCEKELLQVVEHQPVFSYLDDPTNPSYALLPKLQEVLVQKALKNPESENSDKKGYTIFKRIPVFMEELKRRLEEEVPKARERFDSGEFGIENRIKKCRTYPIYEFVRSEVGTELLSGAKKVSPGEDIEKVHEAINEGKLGEVLIKCLANWKETCPSV
>tung.mrna.scaffold1775.00007.001
MATNSISPSIEAIPLCNSFVSTPSPHWKMAADSLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAIARRSEVGVQLNELAARDRVAKSANWVAVTISRGTDTYGVTTGFGATSHRRTNKTGDLQTELIRFLNAGVIGKENLPTSYSKAAMLVRTNTLMQGYSVIRKTLYMAEDGSLLESRFCEKELLQVVEHQPVFSYLDDPTNPSYALLPKLQEVLVQKALKNPESENSDKKGYTIFKRIPVFMEELKRRLEEEVPKARERFDSGEFGIENRIKKCRTYPIYEFVRSEVGTELLSGAKKVSPGEDIEKVHEAINEGKLGEVLIKCLANWKETCPSV
>tung.mrna.scaffold245.00102.001
MATNSISPSIEAIPLCNSFVSTPSPHWKMAADSLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAIARRSEVGVQLNELAARDRVAKSANWVAVTISRGTDTYGVTTGFGATSHRRTNKTGDLQTELIRFLNAGVIGKENLPTSYSKAAMLVRTNTLMQGYSGIRWEILESIAKLMNQNLIPKLPLRGTITASGDLVPLSYIAGLLTGRHNSKVATLQGQEITAIEALKRAGIQAPFELQAKEGLALVNGTAVGSAVAATVCFDANILALLAEILSALFCEVMHGKPEYTDPLTHQLKHHPGQIEAAAIMKHLLDESDYMKEAKIRHEKDPLTKPKQDRYAIRTSPQWLGPQIEVIRAATHSIEREINSVNDNPLIDVARDLALHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATFVVAICQAIDLRHLEENMREVVKQIVRQVIRKTLYMAEDGSLLESRFCEKELLQVVEHQPVFSYLDDPTNPSYALLPKLQEVLVQKALKDPKSENSDKNGYTIFKRIPVFMEELKRRLEEEVPKARERFDSGEFGIENRIKKCRTYPIYEFVRSEVGTELLSGAKKVSPGEDIEKVHEAINEGKLGEVLIKCLANWKETCPSV
>tung.mrna.scaffold245.00101.001
MATNSISSSIEAIPLCNSFVSTPSPHWKMAAESLQCDHFYEVSQMISQFSNAKTVDIQGTSLSVAQVTAVARRSEVSVQLNELAARDRVAKSANWVAVNISRGTDTYXHRSIKYLQQNNSPTTMRQLFS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University