Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Vaccinium macrocarpon
Species name
American cranberry (Vaccinium macrocarpon) is a species of eudicot in the family Ericaceae (heath family).
Species info
Gene sequence info
Vaccinium_macrocarpon.ANR.fa
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>vmacro17688-RA_Vaccinium_macrocarpon_Stevens_v1
MLKLTFWHQKLYPSSYVLNSRHISNTYTISVNLNLQLKVATVFHVATPLQHNTRTKAYIKSKMLAEMEALWYNEIGNGKMEVVSLLCGLVGGDNTLLSYLSASVAVNLSQITGDLTSYDNLRFLHELLGSIPLAQIDDVCKAHIFCIEKESIQGRFLCASADPTLKDMANYYKKNYHVHEAHIFCIKKESLRGRFLCASADPTLQDMANYYKKNYPQVKISQK
>vmacro19265-RA_Vaccinium_macrocarpon_Stevens_v1
MSGAGKVVCVTGASGYIASWIVKLLLDRGYTVKATVRSLSDPNKTNHLLSLHGAKERLQLFEANLMEEGSFDSVVDGCEGVFHTASPCVLQVNNPQVYYDTSIGFSFHNEFAGCVSMSVCTEVAMVTHAGFMLKICGDAEGLTDCLNTTSKPLAFELLTAELHKEELIKPAVKGTQNVLCSCAKVPSIKRVVVTSSMASVAFNNRPRNPDVVVDETWYSDPVYCEESKLFYHLSKTLAEDAAWKLSRENGIDLVTINRAWSSLAANSQFNFRSISEPHKKWYTSATFEMMHMISSCMDFMDTVTQIVLLSDNVLIICYPYEARNLLDAGTGLFSDGVYRYVDVRDVALAHIQAFEVSSASGRYCLVGTVSTTTETLNILRKFYPALNLPEMGEIENPVRPPYQVSKKKVESLSIHFTPLEVTLKDTVESLKEKNFLHF
>vmacro11438-RA_Vaccinium_macrocarpon_Stevens_v1
MEKRNCIKVCVTGGSGFIGSWLVKKLLEKGYTVHATLLNLNEELEVGLVKSLPYADTRLVLFQADIYNPSEFEPAIEGCHYVFHVATPLQHNAQSTKYKDTTEAAIAGLKRIADSCIRSKTVKRLIYTASVMASSPLKEDGSGYKSSLDESCWTPLNRSFTSSNDFLLLEYLQNKSNDQLDLIDRGPAVPSAYTKSKTLAEMEALRYNEIGNGKLEVVSLLCGLVGGDNTLLSYLPASVAVILSQITRDLTSYGSLRFLHKLLGSIPLAHIDDVREAHIFCIEKESLRGRFLCTSADPTLKDMAIYYKENYPQVKISQKYFLILLMRGPDRGSSCESTKLEEMGFECKYDMKKILDDCVACLSRIEALSSN
>vmacro10555-RA_Vaccinium_macrocarpon_Stevens_v1
MPEYCITGGTGFIAAYLVKSLLEKGHTVRTTVRDSGDVEKVGFLWELSGAKERLKIFKADLMEERSFDQAIEGVDGVFHTASPVLVPYDQNIQAKFSWNLFVRSFMILLILQETLIDPCIKGTLNVLSSCTRATNVRRVVLTSSCSSVRYRDDVQQVSPLNETHWSDLEYCKRNNLWYAYAKTVAEKEAWRVAEESGIDLVVVNPSFVVGPLLAPAPTSTLQWILAIIKGLIGEYPNTTVGFVHIDDVVAAHLLAMEKSEASGRLICSSSVAHWSVIIEMLRAKYPSYPFEDKCSSQNGDNNPHSMDSSKIIQLGLPPLKTLDQMFGDCIKSFQGKGFL
>vmacro11439-RA_Vaccinium_macrocarpon_Stevens_v1
MEERNSIKVCVTGGSGYIASCFVKKLLEKGYTVHATLRNLDEKIKVVPLKSLPHADTRLVLFQADIYNPSEFEAAIEGCHYVFHLATPLQHNAKSTKYKDTIEASIAAVKSIADCCIRSQSVKRLIYTASVMSTSALKEDSAGFKSSFDESCWTPLNFSSIFAQDNVLEYAKSKTLAEKEVLSYNEIENGKMEVVSLPCGLVGGDNSLLSLLPLSVNVCISQITGNHSMFKEGAPDIGSSCDNTKLMRMGFEYKYGVKDTLDGCVDLGRRLQAFSSI
>vmacro11440-RA_Vaccinium_macrocarpon_Stevens_v1
MEERNSTRVCVTGGSGYIASWLVKKLLEKGYTVHATLRNLDEKIKVDPLKSLPYADTRLVLFQADIYNPSEFEPAIEGCHYVFHLATPLQHNSQSSKYKDTAEAAIAGVKSIADCCIRSETVKRLIYTASMMSLSPLKEDRTGYKSSFDESCWTPLNFSSIVAPDYALEYTNSKTLAEKEVLRYNEIENGKLEVVSLPCGLVGGDNTLLPKLPLSVNAFLSPITGNLSLYDSLRFLQELLGSIPLLHIDDVCEAHIFCLEKESLRGRFMCIAVDTSVQEIASYYQENYPLFKISHE
>vmacro04143-RA_Vaccinium_macrocarpon_Stevens_v1
MSGVGKTVCVTGASGYIASWLVRFLLQRGYTVKASVRDPSLRLLVGYVTRQGGASLKPYLPLMRCIHLTTPAPWRLGTFVMEHPLLKRYYKRTLIHAFKHRIVFNISKYCCYNYGRMEPVRHEAINLKFGDPKKTEHLNSLDGAKERLHLFKANLLEEGSFDAAVDGCVGVFHTASPFYHDVTDPQVELIDPALKGTLNVLGSCAKSSSLKRVVLTSSIAAVAYNGRPRTPEVVVDESWFSDPEVCRQNKVRRALIVLPLNTFLLHMEALT
>vmacro21663-RA_Vaccinium_macrocarpon_Stevens_v1
MEGENRGSRTTVCVAGASGFIGSWLVMRLLKRGHFVRATVRDPEPYGARRCYKITRFVENRSYFSEQNEVIKPTVNGVLNIMRSCCKAKTVKRLVYTSTTGTVAVQEHPQPESNESFWTDIDFCKTQKMTAWVITDYVSVPKFLVIPFMRALSISLGIQIFFIFQMSFVAKTMAEKAAWEFARENGLDLVTIHPSVVIGPFITPSRPLSVDISLSVITNLAKSLSQRYPEYNIPIRFDGVDELLKSVPCSSKKLMDLGFKFKYNPEEHDVGDLVAEAIESCREKGPMPV
>vmacro11778-RA_Vaccinium_macrocarpon_Stevens_v1
MFHFVFFSQLEDSYARKYFIEPAINGTLNLLKACSKTESVKRVVFTSSISTLTAQDSSGKWRSVVNESCLNSVDHVWKTKPSGWIYVLSKLLTEEAAFEFAKQNKIDLVSVIAPTTAGPFLTSTVPSSIQVLLSPITGEEHSSIPSEISSKKLRDLGFNFKYDLEDVITDIVNSCIDCGFLCPIVKPDNANVAF
>vmacro04427-RA_Vaccinium_macrocarpon_Stevens_v1
MIEPAITGTRNVLNACLKAIVNKVVVVSSVAAVMLNPNWPKDHPMDENSWSDTEFCKSTEKLISFELGWKVGEINAMNSRVQQLVDVRDFAEALFLLYEKLEANGRYICSSYTIRPQALVEELKAMYPNYNYPKIFTEKQGSQFDFNCKKLQDFGWMYRPLEESIADAMKNFEESGLLVGT
>vmacro20783-RA_Vaccinium_macrocarpon_Stevens_v1
MSGAGKVVCVTGASGYIASWIVKLLLDGGYTVKATVRSLSDPKKIDHLLSLHGAKERLQLFEANLMEEGSFDSVVDGCEGVFHTASPCVFEVSNPQVYYDTSIGPFFHYEFAGCVSPCQLVISNDILGNWNHSMHWDAGTFQYSEIKQNGGALAPLPLCGQATLQALNVNKLASRRLAFELLAAELHKVVASDSKFSVGPAEEEDLIKPAVKGTQNVLCSCAKVPSIKRVVLTSSMASVATNNRPKNPDVVVDETWFSDPVYCEESRVGKCSDLFYQLSKTLAEDAAWKFSRENGIDLVTINPGYVIGPLLQPTLNLTSEATLNLIKNGTGLFSDGFYRCVDVRDVALAHIRAFEVSSASGRYCLVGTVTSTIETLNILHKLYPALDLPERYVASICININVISGSKGENENPVGPPYQVSRKKAETLIIHFTPLEVTLKDTVESLKEKNFLHF
MLKLTFWHQKLYPSSYVLNSRHISNTYTISVNLNLQLKVATVFHVATPLQHNTRTKAYIKSKMLAEMEALWYNEIGNGKMEVVSLLCGLVGGDNTLLSYLSASVAVNLSQITGDLTSYDNLRFLHELLGSIPLAQIDDVCKAHIFCIEKESIQGRFLCASADPTLKDMANYYKKNYHVHEAHIFCIKKESLRGRFLCASADPTLQDMANYYKKNYPQVKISQK
>vmacro19265-RA_Vaccinium_macrocarpon_Stevens_v1
MSGAGKVVCVTGASGYIASWIVKLLLDRGYTVKATVRSLSDPNKTNHLLSLHGAKERLQLFEANLMEEGSFDSVVDGCEGVFHTASPCVLQVNNPQVYYDTSIGFSFHNEFAGCVSMSVCTEVAMVTHAGFMLKICGDAEGLTDCLNTTSKPLAFELLTAELHKEELIKPAVKGTQNVLCSCAKVPSIKRVVVTSSMASVAFNNRPRNPDVVVDETWYSDPVYCEESKLFYHLSKTLAEDAAWKLSRENGIDLVTINRAWSSLAANSQFNFRSISEPHKKWYTSATFEMMHMISSCMDFMDTVTQIVLLSDNVLIICYPYEARNLLDAGTGLFSDGVYRYVDVRDVALAHIQAFEVSSASGRYCLVGTVSTTTETLNILRKFYPALNLPEMGEIENPVRPPYQVSKKKVESLSIHFTPLEVTLKDTVESLKEKNFLHF
>vmacro11438-RA_Vaccinium_macrocarpon_Stevens_v1
MEKRNCIKVCVTGGSGFIGSWLVKKLLEKGYTVHATLLNLNEELEVGLVKSLPYADTRLVLFQADIYNPSEFEPAIEGCHYVFHVATPLQHNAQSTKYKDTTEAAIAGLKRIADSCIRSKTVKRLIYTASVMASSPLKEDGSGYKSSLDESCWTPLNRSFTSSNDFLLLEYLQNKSNDQLDLIDRGPAVPSAYTKSKTLAEMEALRYNEIGNGKLEVVSLLCGLVGGDNTLLSYLPASVAVILSQITRDLTSYGSLRFLHKLLGSIPLAHIDDVREAHIFCIEKESLRGRFLCTSADPTLKDMAIYYKENYPQVKISQKYFLILLMRGPDRGSSCESTKLEEMGFECKYDMKKILDDCVACLSRIEALSSN
>vmacro10555-RA_Vaccinium_macrocarpon_Stevens_v1
MPEYCITGGTGFIAAYLVKSLLEKGHTVRTTVRDSGDVEKVGFLWELSGAKERLKIFKADLMEERSFDQAIEGVDGVFHTASPVLVPYDQNIQAKFSWNLFVRSFMILLILQETLIDPCIKGTLNVLSSCTRATNVRRVVLTSSCSSVRYRDDVQQVSPLNETHWSDLEYCKRNNLWYAYAKTVAEKEAWRVAEESGIDLVVVNPSFVVGPLLAPAPTSTLQWILAIIKGLIGEYPNTTVGFVHIDDVVAAHLLAMEKSEASGRLICSSSVAHWSVIIEMLRAKYPSYPFEDKCSSQNGDNNPHSMDSSKIIQLGLPPLKTLDQMFGDCIKSFQGKGFL
>vmacro11439-RA_Vaccinium_macrocarpon_Stevens_v1
MEERNSIKVCVTGGSGYIASCFVKKLLEKGYTVHATLRNLDEKIKVVPLKSLPHADTRLVLFQADIYNPSEFEAAIEGCHYVFHLATPLQHNAKSTKYKDTIEASIAAVKSIADCCIRSQSVKRLIYTASVMSTSALKEDSAGFKSSFDESCWTPLNFSSIFAQDNVLEYAKSKTLAEKEVLSYNEIENGKMEVVSLPCGLVGGDNSLLSLLPLSVNVCISQITGNHSMFKEGAPDIGSSCDNTKLMRMGFEYKYGVKDTLDGCVDLGRRLQAFSSI
>vmacro11440-RA_Vaccinium_macrocarpon_Stevens_v1
MEERNSTRVCVTGGSGYIASWLVKKLLEKGYTVHATLRNLDEKIKVDPLKSLPYADTRLVLFQADIYNPSEFEPAIEGCHYVFHLATPLQHNSQSSKYKDTAEAAIAGVKSIADCCIRSETVKRLIYTASMMSLSPLKEDRTGYKSSFDESCWTPLNFSSIVAPDYALEYTNSKTLAEKEVLRYNEIENGKLEVVSLPCGLVGGDNTLLPKLPLSVNAFLSPITGNLSLYDSLRFLQELLGSIPLLHIDDVCEAHIFCLEKESLRGRFMCIAVDTSVQEIASYYQENYPLFKISHE
>vmacro04143-RA_Vaccinium_macrocarpon_Stevens_v1
MSGVGKTVCVTGASGYIASWLVRFLLQRGYTVKASVRDPSLRLLVGYVTRQGGASLKPYLPLMRCIHLTTPAPWRLGTFVMEHPLLKRYYKRTLIHAFKHRIVFNISKYCCYNYGRMEPVRHEAINLKFGDPKKTEHLNSLDGAKERLHLFKANLLEEGSFDAAVDGCVGVFHTASPFYHDVTDPQVELIDPALKGTLNVLGSCAKSSSLKRVVLTSSIAAVAYNGRPRTPEVVVDESWFSDPEVCRQNKVRRALIVLPLNTFLLHMEALT
>vmacro21663-RA_Vaccinium_macrocarpon_Stevens_v1
MEGENRGSRTTVCVAGASGFIGSWLVMRLLKRGHFVRATVRDPEPYGARRCYKITRFVENRSYFSEQNEVIKPTVNGVLNIMRSCCKAKTVKRLVYTSTTGTVAVQEHPQPESNESFWTDIDFCKTQKMTAWVITDYVSVPKFLVIPFMRALSISLGIQIFFIFQMSFVAKTMAEKAAWEFARENGLDLVTIHPSVVIGPFITPSRPLSVDISLSVITNLAKSLSQRYPEYNIPIRFDGVDELLKSVPCSSKKLMDLGFKFKYNPEEHDVGDLVAEAIESCREKGPMPV
>vmacro11778-RA_Vaccinium_macrocarpon_Stevens_v1
MFHFVFFSQLEDSYARKYFIEPAINGTLNLLKACSKTESVKRVVFTSSISTLTAQDSSGKWRSVVNESCLNSVDHVWKTKPSGWIYVLSKLLTEEAAFEFAKQNKIDLVSVIAPTTAGPFLTSTVPSSIQVLLSPITGEEHSSIPSEISSKKLRDLGFNFKYDLEDVITDIVNSCIDCGFLCPIVKPDNANVAF
>vmacro04427-RA_Vaccinium_macrocarpon_Stevens_v1
MIEPAITGTRNVLNACLKAIVNKVVVVSSVAAVMLNPNWPKDHPMDENSWSDTEFCKSTEKLISFELGWKVGEINAMNSRVQQLVDVRDFAEALFLLYEKLEANGRYICSSYTIRPQALVEELKAMYPNYNYPKIFTEKQGSQFDFNCKKLQDFGWMYRPLEESIADAMKNFEESGLLVGT
>vmacro20783-RA_Vaccinium_macrocarpon_Stevens_v1
MSGAGKVVCVTGASGYIASWIVKLLLDGGYTVKATVRSLSDPKKIDHLLSLHGAKERLQLFEANLMEEGSFDSVVDGCEGVFHTASPCVFEVSNPQVYYDTSIGPFFHYEFAGCVSPCQLVISNDILGNWNHSMHWDAGTFQYSEIKQNGGALAPLPLCGQATLQALNVNKLASRRLAFELLAAELHKVVASDSKFSVGPAEEEDLIKPAVKGTQNVLCSCAKVPSIKRVVLTSSMASVATNNRPKNPDVVVDETWFSDPVYCEESRVGKCSDLFYQLSKTLAEDAAWKFSRENGIDLVTINPGYVIGPLLQPTLNLTSEATLNLIKNGTGLFSDGFYRCVDVRDVALAHIRAFEVSSASGRYCLVGTVTSTIETLNILHKLYPALDLPERYVASICININVISGSKGENENPVGPPYQVSRKKAETLIIHFTPLEVTLKDTVESLKEKNFLHF
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University