Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Acer yangbiense
Species name
Acer yangbiense is a species of eudicot in the family Sapindaceae (soapberry family).Acer yangbiense is a newly described critically endangered endemic maple tree confined to Yangbi County in Yunnan Province in Southwest China. It was included in a programme for rescuing the most threatened species in China, focusing on plant species with extremely small populations (PSESP).
Species info
Gene sequence info
Acer_yangbiense.FLS.fa
Download
>Acyan02G0072600.1
MHEKPLDFRAPPPSPIATGRRSSFANEEVLSEFLEHSLRVPDLILPDKVFPREKFIENPPRIDFQSLNSEESDSVSRILDSITTIGCFQLVNYGIPSDVIKSTLAVAAGIFGLPSEKRAAVTRSPEKAYGFEEVHWEEEEEDDSELSEEFVWCRDECFKLEMEGIWPLQYSKFSEKMEALLMEIETVGEKILQVLKENSTRKSVLYGDDHHEVMGSVCYLYRHGRNIIPEDRRANLLRYDVIRMLMRGVDISHCLSLHVCDGASEFHVYSKKGWVSFCPDKDALVVTAGDQLQALSGGQYKHVIGRAIFEAEKEDCISMAFLYSPPSIIITTTTTTSSNLDKGNTISLTQQLVIAIFSTLLYHFLVYFYKKN
>Acyan06G0106800.1
MAVESVQAVASICNLKDSIPSEFIRPEIEQPAITTYHGIVPEIPTIDISDPDVEKVVGLMAKASREWGIFQIVNHGIPDDVIRRLQAVGRQFFELPQEEKEAYAKQPGSQSIEGYGSKLQKEIEGKKSWVDHLFHKIWPPSSINYRFWPKIPSSYREVNEEYAKYMGEVVDKLFQGLSIGLGLERHRLKEASGGEEVEYMMKINYYPPCPRPDLALGVVAHTDLSAITILVPNEVPGLQVFKDDRWIDAKYIPNALIIHIGDQIEILSNGKYKAVLHRTTVDKERTRMSWPVFLEPPSELVVGPIADLLDEENPPKYKAKKFKDYSFCKLNKIPQ
>Acyan08G0007900.1
MELPVIDLAPYLSIDGGDDGSMVGSELGGGLSELCREVSQILKETGALLVKDPRCTVEDNDRFIDMMEKYFESPSEFKRLQERPNLHYQVGVTPEGVEVPRSLVDDEMQAKLNAMPKESQPSLPKGPDCKWRYMWRAGPRPSKTRFQELNSEPVIPEGFPEWKETMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSRYGQEGIVFAGYHYDLNFLTIHGRSRFPGLNIWLSNGQKVEVKVPVGCLLIQAGKQIEWLTAGECQAGMHEVIVTNRTTEAIKLASAQNRSLWRVSSTLFAHIASDATLKPLGHFAESPLAGKYPSMCAGEFVEQELAVINLKGNKEEP
>Acyan08G0062600.1
MDLPVIDLAPYLSVGGDGSKVTSELGELCREVSRILREMGALLVKDPRCTVEDNDRFINMMEKYFESPPEFKRLQERPKFHFQVGVTPEGIEIPKSMVNEEMQAKIKAMPKESQPSIPKGPDCKWRYMWRSGPQTSKSRFRVQELNAEPVIPEGFPEWKETMDSVAYKWLSAIEVVAEMAAIGFGLPKDAFTSIMKQAPHYLAPTGSDLRRYGQEGSVFAGYHYDLNFLTIHGRSSFPGLNIWLNNGQKVEVEVPVGYLLLQTGKQMEWLTAGECQAGMHEVIVTKRTIEAIKQASAQNRSLWRVSSTFFSHIASEAALKPLGHFAESPLASKYPPMCAGEYVERELAFQRE
>Acyan10G0116800.1
MMERSVQGIALKFKEKIPEEFIRSKDEQPGITTVEGGILEVPVIDLRNPDEDKILESIVDASQKWGMFQVVNHEIPSEVISKLQQVGREFFELPEEEKEKEVYVKPVEVDHTGTLSLEGYGTKLQREVLGKKGWVDHLFHKIWPPSAVNYHFWPKNPSYREANEEYAKWVQGVGDKLLKWLSLGLGLEEEELKKALGGENLVYLTKINYYPPCPQPEVVLGVVAHTDMNSITILVPNDVQGLQAFRDDLWYDVKYIS
>Acyan10G0117300.1
MEVERVQALAYGGLNELPAQFIRPAHERPENSKPIEGIKVPVISLSQPDHNLLVKEIFEASNQWGFFLITDHGISPSLIEQLQEVGQEFFKLPQEEREAHANDPSTGKFEGYGTKMTKNQDEKVEWVDYFFHLMAPKTKVNFDFWPKNPPSYREVTEEYNKEILRITYQLLELLSEGLGLSGKILESHLGGEEILTEMKINMYPPCPQPQLALGVEPHTDMSALTILVPNDVPGLQVWKDGNWVAVDYLPNALFVHVGDQVEVLSNGKYKSVLHRSVVNKERMRMSWAVFCTPPHEALIGPLPEIMDDQNPAKFSTKTYAEYRYRKFNKIPQ
MHEKPLDFRAPPPSPIATGRRSSFANEEVLSEFLEHSLRVPDLILPDKVFPREKFIENPPRIDFQSLNSEESDSVSRILDSITTIGCFQLVNYGIPSDVIKSTLAVAAGIFGLPSEKRAAVTRSPEKAYGFEEVHWEEEEEDDSELSEEFVWCRDECFKLEMEGIWPLQYSKFSEKMEALLMEIETVGEKILQVLKENSTRKSVLYGDDHHEVMGSVCYLYRHGRNIIPEDRRANLLRYDVIRMLMRGVDISHCLSLHVCDGASEFHVYSKKGWVSFCPDKDALVVTAGDQLQALSGGQYKHVIGRAIFEAEKEDCISMAFLYSPPSIIITTTTTTSSNLDKGNTISLTQQLVIAIFSTLLYHFLVYFYKKN
>Acyan06G0106800.1
MAVESVQAVASICNLKDSIPSEFIRPEIEQPAITTYHGIVPEIPTIDISDPDVEKVVGLMAKASREWGIFQIVNHGIPDDVIRRLQAVGRQFFELPQEEKEAYAKQPGSQSIEGYGSKLQKEIEGKKSWVDHLFHKIWPPSSINYRFWPKIPSSYREVNEEYAKYMGEVVDKLFQGLSIGLGLERHRLKEASGGEEVEYMMKINYYPPCPRPDLALGVVAHTDLSAITILVPNEVPGLQVFKDDRWIDAKYIPNALIIHIGDQIEILSNGKYKAVLHRTTVDKERTRMSWPVFLEPPSELVVGPIADLLDEENPPKYKAKKFKDYSFCKLNKIPQ
>Acyan08G0007900.1
MELPVIDLAPYLSIDGGDDGSMVGSELGGGLSELCREVSQILKETGALLVKDPRCTVEDNDRFIDMMEKYFESPSEFKRLQERPNLHYQVGVTPEGVEVPRSLVDDEMQAKLNAMPKESQPSLPKGPDCKWRYMWRAGPRPSKTRFQELNSEPVIPEGFPEWKETMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSRYGQEGIVFAGYHYDLNFLTIHGRSRFPGLNIWLSNGQKVEVKVPVGCLLIQAGKQIEWLTAGECQAGMHEVIVTNRTTEAIKLASAQNRSLWRVSSTLFAHIASDATLKPLGHFAESPLAGKYPSMCAGEFVEQELAVINLKGNKEEP
>Acyan08G0062600.1
MDLPVIDLAPYLSVGGDGSKVTSELGELCREVSRILREMGALLVKDPRCTVEDNDRFINMMEKYFESPPEFKRLQERPKFHFQVGVTPEGIEIPKSMVNEEMQAKIKAMPKESQPSIPKGPDCKWRYMWRSGPQTSKSRFRVQELNAEPVIPEGFPEWKETMDSVAYKWLSAIEVVAEMAAIGFGLPKDAFTSIMKQAPHYLAPTGSDLRRYGQEGSVFAGYHYDLNFLTIHGRSSFPGLNIWLNNGQKVEVEVPVGYLLLQTGKQMEWLTAGECQAGMHEVIVTKRTIEAIKQASAQNRSLWRVSSTFFSHIASEAALKPLGHFAESPLASKYPPMCAGEYVERELAFQRE
>Acyan10G0116800.1
MMERSVQGIALKFKEKIPEEFIRSKDEQPGITTVEGGILEVPVIDLRNPDEDKILESIVDASQKWGMFQVVNHEIPSEVISKLQQVGREFFELPEEEKEKEVYVKPVEVDHTGTLSLEGYGTKLQREVLGKKGWVDHLFHKIWPPSAVNYHFWPKNPSYREANEEYAKWVQGVGDKLLKWLSLGLGLEEEELKKALGGENLVYLTKINYYPPCPQPEVVLGVVAHTDMNSITILVPNDVQGLQAFRDDLWYDVKYIS
>Acyan10G0117300.1
MEVERVQALAYGGLNELPAQFIRPAHERPENSKPIEGIKVPVISLSQPDHNLLVKEIFEASNQWGFFLITDHGISPSLIEQLQEVGQEFFKLPQEEREAHANDPSTGKFEGYGTKMTKNQDEKVEWVDYFFHLMAPKTKVNFDFWPKNPPSYREVTEEYNKEILRITYQLLELLSEGLGLSGKILESHLGGEEILTEMKINMYPPCPQPQLALGVEPHTDMSALTILVPNDVPGLQVWKDGNWVAVDYLPNALFVHVGDQVEVLSNGKYKSVLHRSVVNKERMRMSWAVFCTPPHEALIGPLPEIMDDQNPAKFSTKTYAEYRYRKFNKIPQ
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University