Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Vaccinium macrocarpon
Species name
American cranberry (Vaccinium macrocarpon) is a species of eudicot in the family Ericaceae (heath family).
Species info
Gene sequence info
Vaccinium_macrocarpon.COMT.fa
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>vmacro17335-RA_Vaccinium_macrocarpon_Stevens_v1
MLSLMLVVRVQCQVDNHQIGHRLVLYPTLCCWFAIGRGAISRDGPTMLSDLSSALGCSPSILHSIMRVSGGRRWRRWDHSPVVGQGGGDGTTLRLLAKAFPWIRGINFDLPHVVSIAPDCVGVVHVGGDMFVSVSKADVAYLKWVLHDWDDDECIQILRNCREAVPKDKGKVIMIEAVVEEDKNDKLEYVRLTLDMVMMKRGLQRNGLNF
>vmacro16740-RA_Vaccinium_macrocarpon_Stevens_v1
MEPFYSNNKEEEEEEKHFNYAMQVLGSSALPMVLKAAIELQVLDIIAKEGPGAQLSPSEIACRLPPSTTLVCNQNAPEMLDRMLRFLTSYSIVTCSVVSSDHPPPLAAVVGQQRLYGLAPAGKYFVPNQDNVSLGPFLSLCQDKVSMNSWYELKGAVVEGGIPFDRVHGMHAFEYPGKDPRFNEVFNKAMINHTTIVLNKILHSSYKGFEQVKILVDVGGGLGVTLNLIISKYSHIKGINFDLPHVIQQAPPYPGVQHVGGDMFESVPKGDAIFMKWILHDWSDDHCLKLLKNCHQALPEDGKVIVVEGLVPVIPDRSDATKCIFRLDLAMMTQSPCGKERTKQEFMALATRAGFSGIRLECFVCNFWVMEIYK
>vmacro16849-RA_Vaccinium_macrocarpon_Stevens_v1
MEQSYSNNEEEENFLYAMQLASSSVLPMVLKAVVELKVFDIIAKAGPGAQLSPSEIASRLPVISNRDAPEMLDRMLCLLASNSILTCSVVSSDHPSSTPAAIVGQTRLYGQGLHGQLVRTKRAVVEGVIPFDRVHGMHAFEYPGRIPVECIRSTDWEQDTMWCYKRQLLRESE
>vmacro11479-RA_Vaccinium_macrocarpon_Stevens_v1
MSSSESQTVTQTFDAEESHRQYVMQLVSALVLLMVLKSVIELGVLEIIAKAGPGALLSPSEIATHLQTDNPEAPLILDLSTHCNDGQQEVNSSSSSKLYGLAPVSKYFIPNQDGVSLSPMLHVIHDKVTVNMWYHLKDAVMEGGLPFNRAYGMNSVDYVRNDPRFPDLSKASTRDYNTIFMKQTLETYKCFKGLNLIVDVGGGQGVVLNMIVSKYPTIKGINFDLTPVIEKAPSYPGIESDTYFVFENVARDMFLSIPKGDSILMKVGDD
>vmacro10164-RA_Vaccinium_macrocarpon_Stevens_v1
MGGSESENQTSSTSTPVGSEEEEEACLFAMQLASASVLPMVLKSAIELDLLETMAKAGPGAFVSPTELAAQLPTQNPEAAVMLDRILRLLTSYSVLNCSLKSLPGGGVERLYALAPVCKYLTKNSDGVSMAPLLLMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILEIYQGFEGLTSLVDVGGGTGATLHMIRSKYPTIKGINFDLPHVIEDAPAYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCAKFLKNCYDALPNNGKVIVAECNLPVSPGTSLASKNVIHIDVIMLAHNPGGKERTKEEFEALAKGAGFEGFRVICSAFNTYLMEFLKKI
>vmacro04146-RA_Vaccinium_macrocarpon_Stevens_v1
MEPFFSDNKEKEEENQFIHAMELIGGSVLPVVLKALIELQVLDILAEAGPGAQLSPSEILSRLPPSATLGSNPNAPEMLDRMLCFLVSHSILTCSVVSPNQASPPAALVRPRRLYGLAPVAKYLVNNQDGCSLAPLLFFSQDKVFMEGWDGLKGAVLEGGIPFARVHGMQLFEYPGKDPRFNEVFNKAMLNVTTIIVKRILNSYKGFEQLQNLVDVAGGLGVTLHLITSKYPHIRAINFDLPHVIKYAPPYPALPANGKVIVAEGILPVIPDRSDATKYICRMDLFIMAGLGGKERTQQEFMALAIGAGFSGIKFECYACNLWVMEFYK
>vmacro20781-RA_Vaccinium_macrocarpon_Stevens_v1
MNLPLPGGGGLGATFGSHSFILHLHPAFKGYCLEEGYVQRDYARTSSEDESAGSELIQVGTYSARVKQPLAICTATYSATTACSSTWVMGNVSRVIGLLEPTCRDVIPGMVSMAGTEVTSPVIPSMNDGPPPPASSKGSSVHYSLHLYICHPGRFREFTPARTPPHSVLDLCFDDDTKSDPLVDQRLYGLAPVAKYFVSKQDGVSLAPFFCLIQDKVFNASWVHGMHSFEYPGKDHRFNESKSCLMLEAVSVSAGLNMITSKYPHIKAINFDLPHVIQHAPHYPGVEHVERDMFESVPAGDAIFLKWILHDWNDDHCLKLLKNCHKALPDNGKIIAVEGILPVIPDKKGDTYILSGNNSAATKVIFQLDVQIMGQCRGGKEWTQQEFLNLATRAGFSGIKLECFVCNQWVMEFYK
>vmacro22698-RA_Vaccinium_macrocarpon_Stevens_v1
MARAINSSSPPPTAAATLNPTSSTPLGIHLDVMEVLKSWMKKGYMTMQDIKEQERLISNHALATWTIGLVALPEPSVHHLDTGAVLEGGIPFHKVHGKPAFEYLRKDPMFNEQVQKLVDVGGGLGATLNLITSKTPHIKAINFDLPHIIQYNVFKEPIYPVKLYARYKQRLSNFGSGVEHVGGDMFESVVKGDAILMKYWILHNWSDNRCLKWLKNCHKALPDNGKIIVVEGVLPAMPDSSGAAKGLFQMDLQMMAQHFGGKERTQEEFLALAIGGGFSGIKFEWFVSDLWIMEFYK
MLSLMLVVRVQCQVDNHQIGHRLVLYPTLCCWFAIGRGAISRDGPTMLSDLSSALGCSPSILHSIMRVSGGRRWRRWDHSPVVGQGGGDGTTLRLLAKAFPWIRGINFDLPHVVSIAPDCVGVVHVGGDMFVSVSKADVAYLKWVLHDWDDDECIQILRNCREAVPKDKGKVIMIEAVVEEDKNDKLEYVRLTLDMVMMKRGLQRNGLNF
>vmacro16740-RA_Vaccinium_macrocarpon_Stevens_v1
MEPFYSNNKEEEEEEKHFNYAMQVLGSSALPMVLKAAIELQVLDIIAKEGPGAQLSPSEIACRLPPSTTLVCNQNAPEMLDRMLRFLTSYSIVTCSVVSSDHPPPLAAVVGQQRLYGLAPAGKYFVPNQDNVSLGPFLSLCQDKVSMNSWYELKGAVVEGGIPFDRVHGMHAFEYPGKDPRFNEVFNKAMINHTTIVLNKILHSSYKGFEQVKILVDVGGGLGVTLNLIISKYSHIKGINFDLPHVIQQAPPYPGVQHVGGDMFESVPKGDAIFMKWILHDWSDDHCLKLLKNCHQALPEDGKVIVVEGLVPVIPDRSDATKCIFRLDLAMMTQSPCGKERTKQEFMALATRAGFSGIRLECFVCNFWVMEIYK
>vmacro16849-RA_Vaccinium_macrocarpon_Stevens_v1
MEQSYSNNEEEENFLYAMQLASSSVLPMVLKAVVELKVFDIIAKAGPGAQLSPSEIASRLPVISNRDAPEMLDRMLCLLASNSILTCSVVSSDHPSSTPAAIVGQTRLYGQGLHGQLVRTKRAVVEGVIPFDRVHGMHAFEYPGRIPVECIRSTDWEQDTMWCYKRQLLRESE
>vmacro11479-RA_Vaccinium_macrocarpon_Stevens_v1
MSSSESQTVTQTFDAEESHRQYVMQLVSALVLLMVLKSVIELGVLEIIAKAGPGALLSPSEIATHLQTDNPEAPLILDLSTHCNDGQQEVNSSSSSKLYGLAPVSKYFIPNQDGVSLSPMLHVIHDKVTVNMWYHLKDAVMEGGLPFNRAYGMNSVDYVRNDPRFPDLSKASTRDYNTIFMKQTLETYKCFKGLNLIVDVGGGQGVVLNMIVSKYPTIKGINFDLTPVIEKAPSYPGIESDTYFVFENVARDMFLSIPKGDSILMKVGDD
>vmacro10164-RA_Vaccinium_macrocarpon_Stevens_v1
MGGSESENQTSSTSTPVGSEEEEEACLFAMQLASASVLPMVLKSAIELDLLETMAKAGPGAFVSPTELAAQLPTQNPEAAVMLDRILRLLTSYSVLNCSLKSLPGGGVERLYALAPVCKYLTKNSDGVSMAPLLLMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNQGMSNHSTITMKKILEIYQGFEGLTSLVDVGGGTGATLHMIRSKYPTIKGINFDLPHVIEDAPAYPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCAKFLKNCYDALPNNGKVIVAECNLPVSPGTSLASKNVIHIDVIMLAHNPGGKERTKEEFEALAKGAGFEGFRVICSAFNTYLMEFLKKI
>vmacro04146-RA_Vaccinium_macrocarpon_Stevens_v1
MEPFFSDNKEKEEENQFIHAMELIGGSVLPVVLKALIELQVLDILAEAGPGAQLSPSEILSRLPPSATLGSNPNAPEMLDRMLCFLVSHSILTCSVVSPNQASPPAALVRPRRLYGLAPVAKYLVNNQDGCSLAPLLFFSQDKVFMEGWDGLKGAVLEGGIPFARVHGMQLFEYPGKDPRFNEVFNKAMLNVTTIIVKRILNSYKGFEQLQNLVDVAGGLGVTLHLITSKYPHIRAINFDLPHVIKYAPPYPALPANGKVIVAEGILPVIPDRSDATKYICRMDLFIMAGLGGKERTQQEFMALAIGAGFSGIKFECYACNLWVMEFYK
>vmacro20781-RA_Vaccinium_macrocarpon_Stevens_v1
MNLPLPGGGGLGATFGSHSFILHLHPAFKGYCLEEGYVQRDYARTSSEDESAGSELIQVGTYSARVKQPLAICTATYSATTACSSTWVMGNVSRVIGLLEPTCRDVIPGMVSMAGTEVTSPVIPSMNDGPPPPASSKGSSVHYSLHLYICHPGRFREFTPARTPPHSVLDLCFDDDTKSDPLVDQRLYGLAPVAKYFVSKQDGVSLAPFFCLIQDKVFNASWVHGMHSFEYPGKDHRFNESKSCLMLEAVSVSAGLNMITSKYPHIKAINFDLPHVIQHAPHYPGVEHVERDMFESVPAGDAIFLKWILHDWNDDHCLKLLKNCHKALPDNGKIIAVEGILPVIPDKKGDTYILSGNNSAATKVIFQLDVQIMGQCRGGKEWTQQEFLNLATRAGFSGIKLECFVCNQWVMEFYK
>vmacro22698-RA_Vaccinium_macrocarpon_Stevens_v1
MARAINSSSPPPTAAATLNPTSSTPLGIHLDVMEVLKSWMKKGYMTMQDIKEQERLISNHALATWTIGLVALPEPSVHHLDTGAVLEGGIPFHKVHGKPAFEYLRKDPMFNEQVQKLVDVGGGLGATLNLITSKTPHIKAINFDLPHIIQYNVFKEPIYPVKLYARYKQRLSNFGSGVEHVGGDMFESVVKGDAILMKYWILHNWSDNRCLKWLKNCHKALPDNGKIIVVEGVLPAMPDSSGAAKGLFQMDLQMMAQHFGGKERTQEEFLALAIGGGFSGIKFEWFVSDLWIMEFYK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University