Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Urochloa fusca
Species name
Urochloa fusca is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Urochloa_fusca.HI4_OMT.fa
Download
>Urofu.2G119900.1.v1.1
MENQEADAEPSSFTRDELLQASIDLTHHALSYVKSMALRCAVQLGVADAIRGAGGEASLDGLAAALSLAPSKLPHLRRVMRVLAASGVFAEAAGGGGGYRLTPVSTLLLTDGGGGRCSWSLQQLVRLELSPFAVSPVTNLAEWFNKDDQSETSFAMTFGTDYWDFCGRNPGFSEFFNGAMACDSRFVMDAVVHEMGGIFDGVTSMVDVAGGTGGAAKAVAAAFPHIKCKVLDLPHVINGIPADDGQVVEFVAGDMMDSIPQADALLLKFVLHDWSDEDCLKILKRCKEAICCREQGGKLIIIEVVVGSSSSRATCHEAQLLFDLFISTMVKGRERDEKEWCKLFKEAGFSHYKVTSVLDIRSVIEVFP
>Urofu.3G212100.1.v1.1
MALNGHSTNHNQALLDAQLELWRSTFAYIKSMALKSALDLRTADAIHHHGGGSGATLPQIVARVMLHPSKTPHLRRLMRVLAATGVFTVQHPPAAGGSGGGDDDDEPMYMLTPVSRLLVGSRSLEPVTALVLHPSIVTPFFELGEWLQRELPDPSIFKLAHGQSLWERADRDAAFDALVNDGMLSNSHFIMGIAIEECACVFRGITSLVDVGGGLGAAAQAIAKAFPDVKCSAPSGTGVRYVGGDMFESIPPSDAMFFKWVLHDWGDEECVMILKNCRKAIPSREGGGKVIIIDIVIGVGPSDLRHKEMQALFDIYMMIVNGKERNEQEWKKIFFKAGFSDYKITPVLGVRSIIEVYP
>Urofu.3G212500.1.v1.1
MALTTTTSSTNQALLDAQLELWNNTFGYIKSMALKSALDLRIADAIHNHGGGATLPQIVATVKLHPSKIPCLRRLMRVLASTGVLSVQNHPSSGSGGEPVYALTPLSRLLVGSQNLSPITAMILHPAFVSPFLELGSWFQKELPESCVFKHTHGKTLWEQADRDAAFDALVNDGMVSDSHFIMDIAINECTGAFQGITSLVDVGGGLGAAAQAIAKAFPGVKCSVLDLDHVVANAPGDTSVQYIAGDMFESVPPADAMFFKWVLHDWGDEDCVKILKNCKKAIPPKEEGGKVIIIDIVLQEGSSNSKNRETQALFDLYIMLVNGIERSEQEWRNIFFEAGFSHYKISPVLGARSIIEVYP
>Urofu.6G048200.1.v1.1
MMCGNGAEREEHEWKRIILEAGFSEFNVVATFGLVSVIEVLP
>Urofu.6G056800.1.v1.1
MALSNEQHGSSDPQALLDAQLQLWHHGLGYVKSMALKAALDLRIPDAIDQHGGSATLPQIVTKLILHPSKIPCLRRLMRVLTVTDVFSAQYPDDGGELVYGLTPASRLLVGSSLNVSPLLNLMLGTVNVSSFFGLGRWFEHEMPDPTLFKMMHGREIWDLTDHDASFSKLFDNAMASDSSFIMDIMVKECSDIFQRITSLVDVAGGIGGATQAIAKAFPHVDCSVLDLSHVVANPPTDTSVKYVTGDMFESIPSANSVFLKWVLHDWDDANCIKILRNCKKAIPSKDEGGKVIILDIVVGAGSSEQTHVETQVLFDLMIMSINGIQRDEQEWKKIIVEAGFSDYKIMPVLGVRSIIEAYP
>Urofu.7G094100.1.v1.1
MAITKDSEELLQAQLELWHHAFGYVKSMALAVALDLGVADAIHRRGGAATLSQILAEIALSPCRLHGLRRLMRALTVAGTFTISTTSSEDGEGGVEAVYELTPASRLLVTDDVAGDDGGEGPAASLSPVLSLVLNPFRVSPLGMGIGAWFRRDDRPEAAPFAVAHGRNMWETAARKPVFNALVNDAMAADSRFLMRIVLRECAEVFDGVGSLVDVAGGLGGAATSVARAFPEMKCSVLDLPHVVANAPSGGSSGVQFVAGDMFQSIPPADAVFLKWILHDWGDDECIKILKNCKQAIPSRDKGGKVIIIDMVVGSGSYDAKKLETQVLYDLLIMGINGVERDEKEWKKIFFEAGFKDYKIMPILGVRSIIELYP
>Urofu.8G113800.1.v1.1
MGSIQEQHSSAGQKALLDAQLDLWHNTFAFIKSVAFKCALELRIADAIHHHGGMATLTKIASEVKLHPSKIPCLRRLMRVLTVTGIFSIAKNPAEDGDSVYSLTPASQLLAGPQNLTPTLSLILHNTFVSPFLSLGSWLEHELPNLALFEMAHGKTVWDVIIHDAAISSLFNAGMIADSRFLMDIVIKECGHVFQGISSLIDVAGGHGAAAQAIAKAFPHIDCSVLDLEQVIANAPADTGLKYIAGDMFESIPPANAVFLKWVMHDWGDSECVTILKNCKKAIPTGDAGGKVIIVDAVVGAGSSTLKHRETQVLYDLFIMVANGIERDEQEWRKIIFEAGFTQYKIVPVLGVRSIIEVYP
MENQEADAEPSSFTRDELLQASIDLTHHALSYVKSMALRCAVQLGVADAIRGAGGEASLDGLAAALSLAPSKLPHLRRVMRVLAASGVFAEAAGGGGGYRLTPVSTLLLTDGGGGRCSWSLQQLVRLELSPFAVSPVTNLAEWFNKDDQSETSFAMTFGTDYWDFCGRNPGFSEFFNGAMACDSRFVMDAVVHEMGGIFDGVTSMVDVAGGTGGAAKAVAAAFPHIKCKVLDLPHVINGIPADDGQVVEFVAGDMMDSIPQADALLLKFVLHDWSDEDCLKILKRCKEAICCREQGGKLIIIEVVVGSSSSRATCHEAQLLFDLFISTMVKGRERDEKEWCKLFKEAGFSHYKVTSVLDIRSVIEVFP
>Urofu.3G212100.1.v1.1
MALNGHSTNHNQALLDAQLELWRSTFAYIKSMALKSALDLRTADAIHHHGGGSGATLPQIVARVMLHPSKTPHLRRLMRVLAATGVFTVQHPPAAGGSGGGDDDDEPMYMLTPVSRLLVGSRSLEPVTALVLHPSIVTPFFELGEWLQRELPDPSIFKLAHGQSLWERADRDAAFDALVNDGMLSNSHFIMGIAIEECACVFRGITSLVDVGGGLGAAAQAIAKAFPDVKCSAPSGTGVRYVGGDMFESIPPSDAMFFKWVLHDWGDEECVMILKNCRKAIPSREGGGKVIIIDIVIGVGPSDLRHKEMQALFDIYMMIVNGKERNEQEWKKIFFKAGFSDYKITPVLGVRSIIEVYP
>Urofu.3G212500.1.v1.1
MALTTTTSSTNQALLDAQLELWNNTFGYIKSMALKSALDLRIADAIHNHGGGATLPQIVATVKLHPSKIPCLRRLMRVLASTGVLSVQNHPSSGSGGEPVYALTPLSRLLVGSQNLSPITAMILHPAFVSPFLELGSWFQKELPESCVFKHTHGKTLWEQADRDAAFDALVNDGMVSDSHFIMDIAINECTGAFQGITSLVDVGGGLGAAAQAIAKAFPGVKCSVLDLDHVVANAPGDTSVQYIAGDMFESVPPADAMFFKWVLHDWGDEDCVKILKNCKKAIPPKEEGGKVIIIDIVLQEGSSNSKNRETQALFDLYIMLVNGIERSEQEWRNIFFEAGFSHYKISPVLGARSIIEVYP
>Urofu.6G048200.1.v1.1
MMCGNGAEREEHEWKRIILEAGFSEFNVVATFGLVSVIEVLP
>Urofu.6G056800.1.v1.1
MALSNEQHGSSDPQALLDAQLQLWHHGLGYVKSMALKAALDLRIPDAIDQHGGSATLPQIVTKLILHPSKIPCLRRLMRVLTVTDVFSAQYPDDGGELVYGLTPASRLLVGSSLNVSPLLNLMLGTVNVSSFFGLGRWFEHEMPDPTLFKMMHGREIWDLTDHDASFSKLFDNAMASDSSFIMDIMVKECSDIFQRITSLVDVAGGIGGATQAIAKAFPHVDCSVLDLSHVVANPPTDTSVKYVTGDMFESIPSANSVFLKWVLHDWDDANCIKILRNCKKAIPSKDEGGKVIILDIVVGAGSSEQTHVETQVLFDLMIMSINGIQRDEQEWKKIIVEAGFSDYKIMPVLGVRSIIEAYP
>Urofu.7G094100.1.v1.1
MAITKDSEELLQAQLELWHHAFGYVKSMALAVALDLGVADAIHRRGGAATLSQILAEIALSPCRLHGLRRLMRALTVAGTFTISTTSSEDGEGGVEAVYELTPASRLLVTDDVAGDDGGEGPAASLSPVLSLVLNPFRVSPLGMGIGAWFRRDDRPEAAPFAVAHGRNMWETAARKPVFNALVNDAMAADSRFLMRIVLRECAEVFDGVGSLVDVAGGLGGAATSVARAFPEMKCSVLDLPHVVANAPSGGSSGVQFVAGDMFQSIPPADAVFLKWILHDWGDDECIKILKNCKQAIPSRDKGGKVIIIDMVVGSGSYDAKKLETQVLYDLLIMGINGVERDEKEWKKIFFEAGFKDYKIMPILGVRSIIELYP
>Urofu.8G113800.1.v1.1
MGSIQEQHSSAGQKALLDAQLDLWHNTFAFIKSVAFKCALELRIADAIHHHGGMATLTKIASEVKLHPSKIPCLRRLMRVLTVTGIFSIAKNPAEDGDSVYSLTPASQLLAGPQNLTPTLSLILHNTFVSPFLSLGSWLEHELPNLALFEMAHGKTVWDVIIHDAAISSLFNAGMIADSRFLMDIVIKECGHVFQGISSLIDVAGGHGAAAQAIAKAFPHIDCSVLDLEQVIANAPADTGLKYIAGDMFESIPPANAVFLKWVMHDWGDSECVTILKNCKKAIPTGDAGGKVIIVDAVVGAGSSTLKHRETQVLYDLFIMVANGIERDEQEWRKIIFEAGFTQYKIVPVLGVRSIIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University