Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Triticum urartu
Species name
Triticum urartu is a species of monocot in the family Poaceae (grass family).
Species info
Gene sequence info
Triticum_urartu.HI4_OMT.fa
Download
>TuG1812G0100003815.01.P04
MPPIQDKHSSQELLQAQVNLWHHALGFVKSMALKCAMELQIPNTIQHHGGLMTPSELATKIGLHPSKLPRLRRLMRVLTISGIFAVHEAASPGKEVVYGLTPTTRLLVGDEVKSNLFPILSLMLDSTVIAPFLGMHSWFLDEYSTSLFKKAHSLNFWEMAEQDDIYNQILNNAMVSDSNFLTDIILRECGDVFLGINSLIDVGGGHGGAARAIVNAFPQMKCTVLDLPHVIAEAPSDVHVSFISGDMFKYIPPENALFLKWVFHDWGDEDCVKLLKNCKEAIPPRDAGGKVINRRYGGWIWAK
>TuG1812G0100003810.01.P01
MAVAREQHILDQGLLDAQLELWHHTLGYVKSMALKSALELGIADAIHRQGGAATLSQIAATATLHPSKISCLRRLMRVLTVSGVFGVEHSGDGGVEGEAVYALTPASRLLVGSASANMVHVTKMLLHPIIVSPFSDLGTWFQNELPEPDLFKVKHGKTFWELANEDPAYNAIVNDGMVSDSSFLMDIAIRECGDVFQGIGSLVDVAGGHGGAAQAISKAFPHVKCSVMDLAHVVAEAPSGTDVEFIAGDMFESVPSAHAVFLKWVMHDWGDEDCIKILKNCKKAIAPKDVGGKVIIVDMVVGAGSRDQKHKETQVMFDLFIMFVNGIERDEHEWKKIIFEAGFRDYKITPVLGVRSIIEVYL
>TuG1812G0100003797.01.P01
MALTVKSTDDQAVLDAEHELWATTFSYIKSMALKSALDLRLADAIHHHGGAATLPQIAARVAVHPSKIPCLRRLMRTLTVSGVFSVVQQQDVVVPAAPVSNGSCNGAVAAEPLYALTPVSRLLVGSWNLVSIITMLLSPNFVTTFLGIGAWFEHALPDPCLFTQAHGEALWTMADHDATFDALINDGMVSDSSFIMDIAMRECGEVFQGIGSLMDVGGGLGAAAQAISRAFPSLECTVMDLDHVVDKAPAGTDVKYVVGDMFESVPPADAVFLKWVLHDWGHEDCVKILKNCKKSIPPRGKGGKVIIIDIVIGAGPSHVKHRELQSMFDLYMMIVDGIERDEQEWKKIFLEAGFSGYKIIPVLGFRSIIEVYP
>TuG1812G0200000302.01.P01
MTLKLLTEVSPQELLGALAELQNQVLGYVKSMSLRCAVDLGIPDAIHRRNGAATLADIATDAKVHPCKVADLQRVMELLTTSGIFTATATPGDGDTVTVVYGLTTACRFLVGWHSLSPMVPFCVNPLVVSSFFSMPEWLKTEPAAAGAGSLFELAHGCSQWEMVSKDAGFNNVLNDSMAADSQVFLEVIIVDKGRIFRGLRSLIDVGGGNGAGTQVIAKAFPRIKCTVMDLPHVVGQAAGDDNLSFVAGDMFESIPSADAVLLKNILHDWGHDDCVKILQRCKEAIPARNAGGKVIIIDMVRGSANGDKKISEMEAIQNLFTMYITGVERDEIEWKRIFSDAGFSDDYKILPVLGPYSVIEIYP
>TuG1812G0200004887.01.P01
MATTSSAELLQAEAELVRHSLGYLKSMALHSAVKLGIPDALHRCGGAASLPDLSSTLALPCSKRPYLSRLMKMLAVEGIFTAVAVDVDVPTASGGEGSVRYGLNPVSRLLVSGSGACLSPCVLMGTSPLFLEASLRLPQWFQRDGQGDQPAFAMAHGESPYGAAGHDTEFNALVNEAMGSDSRHMAELVVRECGEVFTGVTSLVDVGGGNGTMAMAIAKAFPHVKCSVLDLPHVVQGVVSTHESVEFAAGDMMEYVPPADAVLLKCVLHNWSDEDCVKILTKCREAIAQGAKAGKVIIIDAVVGSPSHSQQVLEAQVLMDMQMMMLFMSKEREELSWQKIFMEAGFSHYKIQPVLGMRSIIQLYP
MPPIQDKHSSQELLQAQVNLWHHALGFVKSMALKCAMELQIPNTIQHHGGLMTPSELATKIGLHPSKLPRLRRLMRVLTISGIFAVHEAASPGKEVVYGLTPTTRLLVGDEVKSNLFPILSLMLDSTVIAPFLGMHSWFLDEYSTSLFKKAHSLNFWEMAEQDDIYNQILNNAMVSDSNFLTDIILRECGDVFLGINSLIDVGGGHGGAARAIVNAFPQMKCTVLDLPHVIAEAPSDVHVSFISGDMFKYIPPENALFLKWVFHDWGDEDCVKLLKNCKEAIPPRDAGGKVINRRYGGWIWAK
>TuG1812G0100003810.01.P01
MAVAREQHILDQGLLDAQLELWHHTLGYVKSMALKSALELGIADAIHRQGGAATLSQIAATATLHPSKISCLRRLMRVLTVSGVFGVEHSGDGGVEGEAVYALTPASRLLVGSASANMVHVTKMLLHPIIVSPFSDLGTWFQNELPEPDLFKVKHGKTFWELANEDPAYNAIVNDGMVSDSSFLMDIAIRECGDVFQGIGSLVDVAGGHGGAAQAISKAFPHVKCSVMDLAHVVAEAPSGTDVEFIAGDMFESVPSAHAVFLKWVMHDWGDEDCIKILKNCKKAIAPKDVGGKVIIVDMVVGAGSRDQKHKETQVMFDLFIMFVNGIERDEHEWKKIIFEAGFRDYKITPVLGVRSIIEVYL
>TuG1812G0100003797.01.P01
MALTVKSTDDQAVLDAEHELWATTFSYIKSMALKSALDLRLADAIHHHGGAATLPQIAARVAVHPSKIPCLRRLMRTLTVSGVFSVVQQQDVVVPAAPVSNGSCNGAVAAEPLYALTPVSRLLVGSWNLVSIITMLLSPNFVTTFLGIGAWFEHALPDPCLFTQAHGEALWTMADHDATFDALINDGMVSDSSFIMDIAMRECGEVFQGIGSLMDVGGGLGAAAQAISRAFPSLECTVMDLDHVVDKAPAGTDVKYVVGDMFESVPPADAVFLKWVLHDWGHEDCVKILKNCKKSIPPRGKGGKVIIIDIVIGAGPSHVKHRELQSMFDLYMMIVDGIERDEQEWKKIFLEAGFSGYKIIPVLGFRSIIEVYP
>TuG1812G0200000302.01.P01
MTLKLLTEVSPQELLGALAELQNQVLGYVKSMSLRCAVDLGIPDAIHRRNGAATLADIATDAKVHPCKVADLQRVMELLTTSGIFTATATPGDGDTVTVVYGLTTACRFLVGWHSLSPMVPFCVNPLVVSSFFSMPEWLKTEPAAAGAGSLFELAHGCSQWEMVSKDAGFNNVLNDSMAADSQVFLEVIIVDKGRIFRGLRSLIDVGGGNGAGTQVIAKAFPRIKCTVMDLPHVVGQAAGDDNLSFVAGDMFESIPSADAVLLKNILHDWGHDDCVKILQRCKEAIPARNAGGKVIIIDMVRGSANGDKKISEMEAIQNLFTMYITGVERDEIEWKRIFSDAGFSDDYKILPVLGPYSVIEIYP
>TuG1812G0200004887.01.P01
MATTSSAELLQAEAELVRHSLGYLKSMALHSAVKLGIPDALHRCGGAASLPDLSSTLALPCSKRPYLSRLMKMLAVEGIFTAVAVDVDVPTASGGEGSVRYGLNPVSRLLVSGSGACLSPCVLMGTSPLFLEASLRLPQWFQRDGQGDQPAFAMAHGESPYGAAGHDTEFNALVNEAMGSDSRHMAELVVRECGEVFTGVTSLVDVGGGNGTMAMAIAKAFPHVKCSVLDLPHVVQGVVSTHESVEFAAGDMMEYVPPADAVLLKCVLHNWSDEDCVKILTKCREAIAQGAKAGKVIIIDAVVGSPSHSQQVLEAQVLMDMQMMMLFMSKEREELSWQKIFMEAGFSHYKIQPVLGMRSIIQLYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University