Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Tripterygium wilfordii
Species name
Tripterygium wilfordii is a species of eudicot in the family Celastraceae (bittersweet family).
Species info
Gene sequence info
Tripterygium_wilfordii.HI4_OMT.fa
Download
>rna-XM_038845872.1
MDLNHSLGVRELLEAQTHLYKHSMNYITSMSLKSIVQLGIPDIIHNHGKPITLHELASAVKVPPGRTKYLNRIMRLMVHSGFFATAKVDCKNQEEEEVAYILTPSSTLLLKHNSICLSPYVHVVLDPSIVASCGFLADWVQGGDGDDDELPVYEKTQGMSFWDYCGQNPTYGKIFNDAMAGDSRSMNLIVRDCEPIFEGVKSLVDVAGGVGLTAKIICEEFPHLKCTVLDLPHVVANLKETENLKYVGGDMLKSIPSGDAILLKWTLHDWSDEDCLKILKNCKEAIGGKGKRGKVIIIDIVLSDQKSDGDQESTELMLIFDIFMMAVLTGKERSEKEWERLFLDAGFSHYKIITSMFGFRSLIEVYP
>rna-XM_038846482.1
MDPNHSPGARELLEAQTHLYKHSMNYITSMSLNSIVQLGIPDIIHNHGNPITLHELASAVKVPPGRTGYLNRIMRLMVHSGFFATTKVDYKNQEEEEVAYILTPSSTLLLKDNSICLSPYVHVVLHPSIVASCGFLADWVQGGDGDGDDDHDKLAVYEKTNGISFWNYCGQNHKFGKMFNDAMTGDSQTMNLIIRDCAPIFEGVKSLVDVAGGVGLTAKIICEEFPHLKCTVLDLPHVVASLKETENLKYVGGDMLKSVPSGDAILLKWTLHDWSDEDCLKILKNCKEAIGGKGKGGKVIIIDSVLSDQRSNGDQESTELMLIFDIMMMALFTGKERSEKEWEKLFLDAGFSHYKIITSMFGFRSLIEVYP
>rna-XM_038833149.1
MCKEETGEDIAEQMALDEGHGACELFQAQTHIYKHCYHYVDSMALKCAVQLGIPDMIHKHDKPITLTELISELHVHPTKASFLQRLMRMLVHSGFFATRKISENQEEEEEGYVLTPTSKLLLKDNVTSLSPFVQAMLDPILIVPWLSLGDWFLGTESVPFETAHGMSFWDYVMHNHEFSKFLSEAMASDSRTITLVVGDHKEIFEGLDSLVDVGGGTGTLARAIAEAFPHITCTVLDLPSVIANQPENKSLKFVGGDMFQSIPPADAIIIKSTLHNWSDEDCIKILKQCRVAISTKGKGGKVMIIDVVINEKKDDKELTKTKLYVDMAMMLICTGRERNEKDFEKLIMEAGFSHYKITPLFGLRSLIEIYP
>rna-XM_038833028.1
MGSKHIEGANEVFQAQTHLYNHILNYINSMSLKCAVQLGISDIIHNHGKPMTLSELVSALQINPTRTGCMYRLMRMLVQSGIFAETKLNDGQNSTAYALTPSSSLLVKDNSNCLSPFVLAMLRPVFVTPYHTLGDWFRSAVPTPFEQAHGMTLWEYGVQDPEFNSLFNEAMSSDSQLMNLVIKDCNQIFEGLSSLVDVGGGNGSISRIISEAFPDIKCTVLDLPHVVADLTGSKNLEFIGGDMFLSIPPADALLLKLIFHGWSDENCVNILKKCREAIPSKGKVIIIDVVINEKKEEHELTETKLLFDMLMMVAVTGRERSEKEWEGLFLEAGFSHYKITPLFGLRSLIEVFP
MDLNHSLGVRELLEAQTHLYKHSMNYITSMSLKSIVQLGIPDIIHNHGKPITLHELASAVKVPPGRTKYLNRIMRLMVHSGFFATAKVDCKNQEEEEVAYILTPSSTLLLKHNSICLSPYVHVVLDPSIVASCGFLADWVQGGDGDDDELPVYEKTQGMSFWDYCGQNPTYGKIFNDAMAGDSRSMNLIVRDCEPIFEGVKSLVDVAGGVGLTAKIICEEFPHLKCTVLDLPHVVANLKETENLKYVGGDMLKSIPSGDAILLKWTLHDWSDEDCLKILKNCKEAIGGKGKRGKVIIIDIVLSDQKSDGDQESTELMLIFDIFMMAVLTGKERSEKEWERLFLDAGFSHYKIITSMFGFRSLIEVYP
>rna-XM_038846482.1
MDPNHSPGARELLEAQTHLYKHSMNYITSMSLNSIVQLGIPDIIHNHGNPITLHELASAVKVPPGRTGYLNRIMRLMVHSGFFATTKVDYKNQEEEEVAYILTPSSTLLLKDNSICLSPYVHVVLHPSIVASCGFLADWVQGGDGDGDDDHDKLAVYEKTNGISFWNYCGQNHKFGKMFNDAMTGDSQTMNLIIRDCAPIFEGVKSLVDVAGGVGLTAKIICEEFPHLKCTVLDLPHVVASLKETENLKYVGGDMLKSVPSGDAILLKWTLHDWSDEDCLKILKNCKEAIGGKGKGGKVIIIDSVLSDQRSNGDQESTELMLIFDIMMMALFTGKERSEKEWEKLFLDAGFSHYKIITSMFGFRSLIEVYP
>rna-XM_038833149.1
MCKEETGEDIAEQMALDEGHGACELFQAQTHIYKHCYHYVDSMALKCAVQLGIPDMIHKHDKPITLTELISELHVHPTKASFLQRLMRMLVHSGFFATRKISENQEEEEEGYVLTPTSKLLLKDNVTSLSPFVQAMLDPILIVPWLSLGDWFLGTESVPFETAHGMSFWDYVMHNHEFSKFLSEAMASDSRTITLVVGDHKEIFEGLDSLVDVGGGTGTLARAIAEAFPHITCTVLDLPSVIANQPENKSLKFVGGDMFQSIPPADAIIIKSTLHNWSDEDCIKILKQCRVAISTKGKGGKVMIIDVVINEKKDDKELTKTKLYVDMAMMLICTGRERNEKDFEKLIMEAGFSHYKITPLFGLRSLIEIYP
>rna-XM_038833028.1
MGSKHIEGANEVFQAQTHLYNHILNYINSMSLKCAVQLGISDIIHNHGKPMTLSELVSALQINPTRTGCMYRLMRMLVQSGIFAETKLNDGQNSTAYALTPSSSLLVKDNSNCLSPFVLAMLRPVFVTPYHTLGDWFRSAVPTPFEQAHGMTLWEYGVQDPEFNSLFNEAMSSDSQLMNLVIKDCNQIFEGLSSLVDVGGGNGSISRIISEAFPDIKCTVLDLPHVVADLTGSKNLEFIGGDMFLSIPPADALLLKLIFHGWSDENCVNILKKCREAIPSKGKVIIIDVVINEKKEEHELTETKLLFDMLMMVAVTGRERSEKEWEGLFLEAGFSHYKITPLFGLRSLIEVFP
Sheng jun & Dong yang Teams, Yunnan agriculture University