Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Trifolium pratense
Species name
Peavine clover (Trifolium pratense) is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Trifolium_pratense.ANAT.fa
Download
>Tp57577_TGAC_v2_mRNA36143.v2
LCIAITYCHVMDDNCCSHFMKTWSLIHGKFEGVDLKSTPCFDRDILRDPKGLLENVFLRDYFEERKTWKNKLIGQTQTQTIKEHHEVVEDYFKATIVFGKEEIERMKKWVLSQWNKNDHEIIKPQFLSKFVVTCGFVWANLVKTRYRNDNNDEKEEFFRFAGDCRDRLGYKIPEGYFGNCLTLCHATVKRKDMKGEDGFVKAVKVIERAISEMKNEPFKNVEEWRDTFKNMFEFGSVLLVT
>Tp57577_TGAC_v2_mRNA32261.v2
MAELIGTEHKLIEQTMIFPSSTTKISLPLTFLDLPLAGPIYVRRQFFYQFPQSTNHFCETTFPSLKQSLSLTLQHFFPLAGNLISPPSPNKPFILCTEKDSVPFTIVESSEDFNHLSANYKLKNIKDFNHLVPKLTHKTIHDDNDNDNEDNNTFVFPLLSLQASVFPNNG
>Tp57577_TGAC_v2_mRNA12593.v2
MSTPPFTLKVLEHCKVTPQLNTISTISSLPLTFLDIPWLLFSPSQPLFFYDFPHSISYFTTTXXXXXXXXXXXESLSLTLQHYFPFAGTFVPSLDVTEPCLKFTLNNSVSFTVAESNSKFKHLCSNHSRDVNEFHPLVPNLQQINSFEVKEFPLLVIQITSFPNSGFSIGLAFHHVVADGRTIHNFIKTWSYCSSSISEASSSLKSLPFYGRRVIIDPKGLREVFLKDWRKRRLVLNEKVRKEVKVDLSNMTRATFIMSASQMEKIKKFIIESCKEKKKSQPLHLSSYVLTSAFFWVCYLKTQQDFVNEKVIHEDVTHFGFIAGGITRLEFEVPKNYFGNCVGFGKVSLRRNDLLGEDGIVIAAKEIGSTIKKLDASILGEGEKWILDWEMLHGSEEHVHITWSPKLKLYELNFGWGKPKKIEEISIDFTRGVSFFESRDFEGGIEIGLALTESKMDVFAFLFNKGLEALP
>Tp57577_TGAC_v2_mRNA24175.v2
MHDEVKIIHQCQVSPPQGSLPSSITLPLSYLDLPYFYCPNIKRIFFYNFPHSTQHFLQTSLPILKDSLSLTLQHFFPFSSKIIFPPKPQNPHILYSQGDSITLTICESKSNFNYLISNSPKDLKIAYPFASLIPSPSIISQDGTLLLPTFAIQITVFPNFGFTICLTFRHEICDGKSFHHFIKFWSSLSKGNLENSSLSLPLHNRDMIHDPKELKQSFLEQIWNSPPNLEYTSANNNLLRHRFILTHHQVENLKKWVRTKSQTIGLETLHLSTFVVTCSLFWICMIKTKSQDDKNKSTIVDCDSNNDFEDNYGFRFLMDLRNHFEVSTNYFGNCLGACVATLPKWKLVGENGICEAVNSIGREIKSFSDPLIGVEKLMFHQRINESGIKSHNMILAVGSPKFNVYETDFGWGKPLLSENIHVDNSSILCFSDSKDEDCAIEVGMVLDGDQIKKFSDVLEAQLRDIVGPK
>Tp57577_TGAC_v2_mRNA24223.v2
MVKTTKSQDSNDFEDNYGFRFPMDLRNHFEISTNYFGNCLGACVATLPKWKLVGENGICEAVNSIGREIKSFNDPLIGVEKLMFRQRINELGINKVT
LCIAITYCHVMDDNCCSHFMKTWSLIHGKFEGVDLKSTPCFDRDILRDPKGLLENVFLRDYFEERKTWKNKLIGQTQTQTIKEHHEVVEDYFKATIVFGKEEIERMKKWVLSQWNKNDHEIIKPQFLSKFVVTCGFVWANLVKTRYRNDNNDEKEEFFRFAGDCRDRLGYKIPEGYFGNCLTLCHATVKRKDMKGEDGFVKAVKVIERAISEMKNEPFKNVEEWRDTFKNMFEFGSVLLVT
>Tp57577_TGAC_v2_mRNA32261.v2
MAELIGTEHKLIEQTMIFPSSTTKISLPLTFLDLPLAGPIYVRRQFFYQFPQSTNHFCETTFPSLKQSLSLTLQHFFPLAGNLISPPSPNKPFILCTEKDSVPFTIVESSEDFNHLSANYKLKNIKDFNHLVPKLTHKTIHDDNDNDNEDNNTFVFPLLSLQASVFPNNG
>Tp57577_TGAC_v2_mRNA12593.v2
MSTPPFTLKVLEHCKVTPQLNTISTISSLPLTFLDIPWLLFSPSQPLFFYDFPHSISYFTTTXXXXXXXXXXXESLSLTLQHYFPFAGTFVPSLDVTEPCLKFTLNNSVSFTVAESNSKFKHLCSNHSRDVNEFHPLVPNLQQINSFEVKEFPLLVIQITSFPNSGFSIGLAFHHVVADGRTIHNFIKTWSYCSSSISEASSSLKSLPFYGRRVIIDPKGLREVFLKDWRKRRLVLNEKVRKEVKVDLSNMTRATFIMSASQMEKIKKFIIESCKEKKKSQPLHLSSYVLTSAFFWVCYLKTQQDFVNEKVIHEDVTHFGFIAGGITRLEFEVPKNYFGNCVGFGKVSLRRNDLLGEDGIVIAAKEIGSTIKKLDASILGEGEKWILDWEMLHGSEEHVHITWSPKLKLYELNFGWGKPKKIEEISIDFTRGVSFFESRDFEGGIEIGLALTESKMDVFAFLFNKGLEALP
>Tp57577_TGAC_v2_mRNA24175.v2
MHDEVKIIHQCQVSPPQGSLPSSITLPLSYLDLPYFYCPNIKRIFFYNFPHSTQHFLQTSLPILKDSLSLTLQHFFPFSSKIIFPPKPQNPHILYSQGDSITLTICESKSNFNYLISNSPKDLKIAYPFASLIPSPSIISQDGTLLLPTFAIQITVFPNFGFTICLTFRHEICDGKSFHHFIKFWSSLSKGNLENSSLSLPLHNRDMIHDPKELKQSFLEQIWNSPPNLEYTSANNNLLRHRFILTHHQVENLKKWVRTKSQTIGLETLHLSTFVVTCSLFWICMIKTKSQDDKNKSTIVDCDSNNDFEDNYGFRFLMDLRNHFEVSTNYFGNCLGACVATLPKWKLVGENGICEAVNSIGREIKSFSDPLIGVEKLMFHQRINESGIKSHNMILAVGSPKFNVYETDFGWGKPLLSENIHVDNSSILCFSDSKDEDCAIEVGMVLDGDQIKKFSDVLEAQLRDIVGPK
>Tp57577_TGAC_v2_mRNA24223.v2
MVKTTKSQDSNDFEDNYGFRFPMDLRNHFEISTNYFGNCLGACVATLPKWKLVGENGICEAVNSIGREIKSFNDPLIGVEKLMFRQRINELGINKVT
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University