Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product

Species information
Trifolium pratense
Species name
Peavine clover (Trifolium pratense) is a species of eudicot in the family Fabaceae (pea family).
Species info

Gene sequence info
Trifolium_pratense.FLS.fa
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>Tp57577_TGAC_v2_mRNA8787.v2
MEVLRVQTIAHQSKEATIPSMFLRSETEQPGTTTVQGVKLEVPIIDFSNEDKAKLQHEIVEASKEWGMFQIVNHDIPNDVIKKLQSVGKEFFELPQEEKEVIAKPVGSDSMEGYGTKLQKEVNGKKGWVDHLFHIIWPTSSINYRFWPNKPASYREVNEEYGKYLRVVADKLFRTMSIGLGLEEHELKEAAGGDNLIHMLKINYYPPCPCPDLVLGVPPHTDMSFLTLLVPNDVQGLQASRHGQWYDVKYVPNALVIHVGDQMEIVSNGKYKAVLHRTTVNKDETRMSWPVFIEPPPEQEVGPHPKLVNVENPPKFKTKKYKDYAYCKLNKIPQ
>Tp57577_TGAC_v2_mRNA19951.v2
MIMSNSYLYVDLPIIDIYEPLQSSSLTTLCKSCKDWGFFHIINHEISKDLCNQLHSLSKLIFNLPSXXXXXXXXXXXXXXXXXXSYTPHFIASPFFESLRVDGPNFYVSAKSSQDVLFEKQNSKFSR
>Tp57577_TGAC_v2_mRNA22483.v2
MFLPVFSNDCMMHRSSLYQGPHLLAPTGSDLEKYNQEGTVFAGYHYDLNFLTIHGRSRFPGLNIWLRNGQKVAVKVPVGCLLIQTGKQIEWLTGGECIAGMHEVVATNRTIDAINLAKEQNRSLWRVSSTLFAHIASDAVLKPLGRFAESPLASKYPPLCAGEYVEQELAIINLKGKK
>Tp57577_TGAC_v2_mRNA18999.v2
MSINIDDNELGIEMEEAFTLCPSLPVPNVQEMVEINPLQVPKRYIRNQEEMEKVNYMPHLSSEIPVIDLTLLSNGNMEELHKLDIACKEWGFFQIVNHGVQKEVMQNMRNVTDEFFKLPIIEKDKYAMLSNDVHGYGHAYVVSEEQTLDWTDTLFLLIYPTRFRQLQFWPKPPLGFKEITEAYSSEVKRVGYEIISSLSVIMGLEEDALVGLHKEVLQGLRVNYYPPCNTPEEVLGLSPXXXXXXXXGEKMSRLSMLEI
>Tp57577_TGAC_v2_mRNA21105.v2
LVWSDHLVLIVHPTRFRKPQFWPENLKDTIEEYSSEIQRVGEELINSLSLILGLEKHVLIGLHKEVLQGFRVNYYPPCNTPEKKIFPFNYF
>Tp57577_TGAC_v2_mRNA13760.v2
LFLLQMAVIWVALAARELFWHHITYYNFLTIQGRSRFHGLNVWLNNERKVEVKIHAGYLLFHTGKQIEWLTCGIFKAVMQEVIVTDKTIEALNVAKEQHCSLW
>Tp57577_TGAC_v2_mRNA30201.v2
MPETSVNFRAPPPSPVASGRRSTVTNNEFLTEFLETSLRVPDLVLPDKIFPKQINHETPPKVDFVSLCFHKDEDLIDVVSESIARFGCFQLHNHGISQQLMAAAVEAAGGIFLVLPGKRDAVTRSPEKLWGFEEYREGEEERSELNEEFIWRKDEELKLKMEGIWPIGYPNFSKKMETLHSRVEKVADKILPIILKNAPKKLTSNGDIIGHGNEVGTLCCIYKHRQDINRNNQWVDSLKYDVIRMLIRGTDYSHSLCLHVCDGSSEFHVYSKKSWLSFCPEQGALVITAGDQIQVLSGGHYKDVIGKAIFKGEREDNISMAFFYSPQNTKRKIQTNTERPISLSQQAILAVILNLMYHVLIFVLKKV
>Tp57577_TGAC_v2_mRNA20570.v2
MEIKRVQTLAFNQLKELPPQFIRSENERPENTKALEGVNVPMISLSQPHNLLVKNITEAASKWGFFVITDHDISPKLIQSLQGVGNEFFSLPQKEKEAYANDPSSGKFEGYGTKMTKNLEEKVEWLDYYFHLMSPSSKVNYEMWPKSPHSYREVVQEYNKEILRVTSNILELLSEGLELENKTLKSSLGGEEIELEMKINMYPPCPQPELALGVEAHTDMSALTLLVSNDVPGLQVWKDDNWVAVNYMQNALFVHIGDQLEVMSCHILVLSNGRYKSVLHRSLVNKERKRMSWAVFVTPPHEVVVGPLPQLVNDQNPPKFSTKTYAEYRYRKFNKIPQ
MEVLRVQTIAHQSKEATIPSMFLRSETEQPGTTTVQGVKLEVPIIDFSNEDKAKLQHEIVEASKEWGMFQIVNHDIPNDVIKKLQSVGKEFFELPQEEKEVIAKPVGSDSMEGYGTKLQKEVNGKKGWVDHLFHIIWPTSSINYRFWPNKPASYREVNEEYGKYLRVVADKLFRTMSIGLGLEEHELKEAAGGDNLIHMLKINYYPPCPCPDLVLGVPPHTDMSFLTLLVPNDVQGLQASRHGQWYDVKYVPNALVIHVGDQMEIVSNGKYKAVLHRTTVNKDETRMSWPVFIEPPPEQEVGPHPKLVNVENPPKFKTKKYKDYAYCKLNKIPQ
>Tp57577_TGAC_v2_mRNA19951.v2
MIMSNSYLYVDLPIIDIYEPLQSSSLTTLCKSCKDWGFFHIINHEISKDLCNQLHSLSKLIFNLPSXXXXXXXXXXXXXXXXXXSYTPHFIASPFFESLRVDGPNFYVSAKSSQDVLFEKQNSKFSR
>Tp57577_TGAC_v2_mRNA22483.v2
MFLPVFSNDCMMHRSSLYQGPHLLAPTGSDLEKYNQEGTVFAGYHYDLNFLTIHGRSRFPGLNIWLRNGQKVAVKVPVGCLLIQTGKQIEWLTGGECIAGMHEVVATNRTIDAINLAKEQNRSLWRVSSTLFAHIASDAVLKPLGRFAESPLASKYPPLCAGEYVEQELAIINLKGKK
>Tp57577_TGAC_v2_mRNA18999.v2
MSINIDDNELGIEMEEAFTLCPSLPVPNVQEMVEINPLQVPKRYIRNQEEMEKVNYMPHLSSEIPVIDLTLLSNGNMEELHKLDIACKEWGFFQIVNHGVQKEVMQNMRNVTDEFFKLPIIEKDKYAMLSNDVHGYGHAYVVSEEQTLDWTDTLFLLIYPTRFRQLQFWPKPPLGFKEITEAYSSEVKRVGYEIISSLSVIMGLEEDALVGLHKEVLQGLRVNYYPPCNTPEEVLGLSPXXXXXXXXGEKMSRLSMLEI
>Tp57577_TGAC_v2_mRNA21105.v2
LVWSDHLVLIVHPTRFRKPQFWPENLKDTIEEYSSEIQRVGEELINSLSLILGLEKHVLIGLHKEVLQGFRVNYYPPCNTPEKKIFPFNYF
>Tp57577_TGAC_v2_mRNA13760.v2
LFLLQMAVIWVALAARELFWHHITYYNFLTIQGRSRFHGLNVWLNNERKVEVKIHAGYLLFHTGKQIEWLTCGIFKAVMQEVIVTDKTIEALNVAKEQHCSLW
>Tp57577_TGAC_v2_mRNA30201.v2
MPETSVNFRAPPPSPVASGRRSTVTNNEFLTEFLETSLRVPDLVLPDKIFPKQINHETPPKVDFVSLCFHKDEDLIDVVSESIARFGCFQLHNHGISQQLMAAAVEAAGGIFLVLPGKRDAVTRSPEKLWGFEEYREGEEERSELNEEFIWRKDEELKLKMEGIWPIGYPNFSKKMETLHSRVEKVADKILPIILKNAPKKLTSNGDIIGHGNEVGTLCCIYKHRQDINRNNQWVDSLKYDVIRMLIRGTDYSHSLCLHVCDGSSEFHVYSKKSWLSFCPEQGALVITAGDQIQVLSGGHYKDVIGKAIFKGEREDNISMAFFYSPQNTKRKIQTNTERPISLSQQAILAVILNLMYHVLIFVLKKV
>Tp57577_TGAC_v2_mRNA20570.v2
MEIKRVQTLAFNQLKELPPQFIRSENERPENTKALEGVNVPMISLSQPHNLLVKNITEAASKWGFFVITDHDISPKLIQSLQGVGNEFFSLPQKEKEAYANDPSSGKFEGYGTKMTKNLEEKVEWLDYYFHLMSPSSKVNYEMWPKSPHSYREVVQEYNKEILRVTSNILELLSEGLELENKTLKSSLGGEEIELEMKINMYPPCPQPELALGVEAHTDMSALTLLVSNDVPGLQVWKDDNWVAVNYMQNALFVHIGDQLEVMSCHILVLSNGRYKSVLHRSLVNKERKRMSWAVFVTPPHEVVVGPLPQLVNDQNPPKFSTKTYAEYRYRKFNKIPQ
Gene tree
Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University