Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Trifolium pratense
Species name
Peavine clover (Trifolium pratense) is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Trifolium_pratense.HI4_OMT.fa
Download
>Tp57577_TGAC_v2_mRNA31644.v2
MDLSMNYGNIEESELYDAQFHLYKHIYNIQSSMAVKFAVELGIADAIHNHGKPITLAELASSLKLQPSKVNILYRLMRFLTHNGFFAKTTSKAKAGEETVYSLTLPSKLLIRGKPMCLAFFASGHPRYMNMWNYTKKWLLEDKEELSLFESANGETFWEMLNKDSESDNLCFFXXXXXXXXXXXXXXXXXXXXXXXXXXVEICSNLSLMQMLFYLRYDFI
>Tp57577_TGAC_v2_mRNA36285.v2
MDLSMNYGNIEESELYDAQIHLYKHIHNIQSSMALKFAVELGIADAIHNHGKPMTLLELASTLKLNPSKVSILYRIMRLLTHNGFFAKTTSKAKAGEETMYSLTPPSKLLIRGKPMCLAFFAGGHPRYMNMWNYTKKWLLEEKEELSLFESANGETFWEMLNKDSESDNLCFFQEAMEADSHMFKLALKDCKHVFEGLETLVDVGGGTGVVAKLIHEVFPDIKYTVLDQPQVVGNLTGNENLNFVGGDMFKFIPHADAVLLKV
>Tp57577_TGAC_v2_mRNA36354.v2
KKTGGDQEEEETTYVLTPPSKLLVRGASTCLASLAGGHPSCMNMWHSSKKWLTEDKEFSLFESANGDNFWNMLNKDPESHKLMSLFQEAMDADSQMFKLALKDCKHVFEGLESLVDVGGGTGVVAKLIHEVFPDIKYTVLDQPQVVGNLTGNKYLNFVGGDMFKSIPQADAVLLKVYDLI
>Tp57577_TGAC_v2_mRNA36283.v2
MFQDAMASDSRIFKISLQENKHVFEGLESLVDVAGGTGGVTKLIHEAFPNIKCTVFDQPQVVANLTGNENLNFVGGDMFKSIPPADAVLLKSFAVAECYPQSVAVLDPQFVHTFLLHFAIA
>Tp57577_TGAC_v2_mRNA15438.v2
MALSTNGSEQSELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPITLPELASSLKLHPSKVNILYRFLRLLTHNGFFAKTTLKNKEGEDEIAYSLTPCSKLLVSGKPTCLSAIVKGALHPSSLDMWSASKKWFNEEKEQTLYECATGESFWDFLNKDSESDTLSMFQDAMASDSRIFKISLQENKHVFEGLESLVDVAGGTGGVTKLIHEAFXXXXXXLLLT
>Tp57577_TGAC_v2_mRNA35290.v2
MNLKSYGNEENHVYQAQIHLFKHIYSFLSSMALKSAVELGVIDVIHKHGKPMTLSELVSSLKLHPSKVSVFYRFMRLLTHNGFFAKTTVLIAKEDGDDEEDEEVEETTYALTPPSMLLINGESPCFTTIINGLFHPSVMDMWHSSKKWFGEENEQALYESANGETFFNFLNKDSEAYILSMFQEAMAADSQMMKFALKDCNHVFEGLESLVDVAGGTGVVSKLILEAFPHIKCTVLDQPQVVANLCGTQNLNFVGGDMFKSIPPADAVLLKWVLHDWNDELCLKILKNCKEAISGKGKKGKIIIIDISIDETSEDHELTKLQLNFDLVMMTLHNGKERNKKEWKKLLYDAGFTNYKITPIFGFKSLIEVYP
>Tp57577_TGAC_v2_mRNA33386.v2
MGEIHSEVTWEKEEEEKEEEEAQVDIWKYIFGFVELSVVKCAIELGIADAIEKHKKSMSLIELSTTLKCDPSY
MDLSMNYGNIEESELYDAQFHLYKHIYNIQSSMAVKFAVELGIADAIHNHGKPITLAELASSLKLQPSKVNILYRLMRFLTHNGFFAKTTSKAKAGEETVYSLTLPSKLLIRGKPMCLAFFASGHPRYMNMWNYTKKWLLEDKEELSLFESANGETFWEMLNKDSESDNLCFFXXXXXXXXXXXXXXXXXXXXXXXXXXVEICSNLSLMQMLFYLRYDFI
>Tp57577_TGAC_v2_mRNA36285.v2
MDLSMNYGNIEESELYDAQIHLYKHIHNIQSSMALKFAVELGIADAIHNHGKPMTLLELASTLKLNPSKVSILYRIMRLLTHNGFFAKTTSKAKAGEETMYSLTPPSKLLIRGKPMCLAFFAGGHPRYMNMWNYTKKWLLEEKEELSLFESANGETFWEMLNKDSESDNLCFFQEAMEADSHMFKLALKDCKHVFEGLETLVDVGGGTGVVAKLIHEVFPDIKYTVLDQPQVVGNLTGNENLNFVGGDMFKFIPHADAVLLKV
>Tp57577_TGAC_v2_mRNA36354.v2
KKTGGDQEEEETTYVLTPPSKLLVRGASTCLASLAGGHPSCMNMWHSSKKWLTEDKEFSLFESANGDNFWNMLNKDPESHKLMSLFQEAMDADSQMFKLALKDCKHVFEGLESLVDVGGGTGVVAKLIHEVFPDIKYTVLDQPQVVGNLTGNKYLNFVGGDMFKSIPQADAVLLKVYDLI
>Tp57577_TGAC_v2_mRNA36283.v2
MFQDAMASDSRIFKISLQENKHVFEGLESLVDVAGGTGGVTKLIHEAFPNIKCTVFDQPQVVANLTGNENLNFVGGDMFKSIPPADAVLLKSFAVAECYPQSVAVLDPQFVHTFLLHFAIA
>Tp57577_TGAC_v2_mRNA15438.v2
MALSTNGSEQSELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPITLPELASSLKLHPSKVNILYRFLRLLTHNGFFAKTTLKNKEGEDEIAYSLTPCSKLLVSGKPTCLSAIVKGALHPSSLDMWSASKKWFNEEKEQTLYECATGESFWDFLNKDSESDTLSMFQDAMASDSRIFKISLQENKHVFEGLESLVDVAGGTGGVTKLIHEAFXXXXXXLLLT
>Tp57577_TGAC_v2_mRNA35290.v2
MNLKSYGNEENHVYQAQIHLFKHIYSFLSSMALKSAVELGVIDVIHKHGKPMTLSELVSSLKLHPSKVSVFYRFMRLLTHNGFFAKTTVLIAKEDGDDEEDEEVEETTYALTPPSMLLINGESPCFTTIINGLFHPSVMDMWHSSKKWFGEENEQALYESANGETFFNFLNKDSEAYILSMFQEAMAADSQMMKFALKDCNHVFEGLESLVDVAGGTGVVSKLILEAFPHIKCTVLDQPQVVANLCGTQNLNFVGGDMFKSIPPADAVLLKWVLHDWNDELCLKILKNCKEAISGKGKKGKIIIIDISIDETSEDHELTKLQLNFDLVMMTLHNGKERNKKEWKKLLYDAGFTNYKITPIFGFKSLIEVYP
>Tp57577_TGAC_v2_mRNA33386.v2
MGEIHSEVTWEKEEEEKEEEEAQVDIWKYIFGFVELSVVKCAIELGIADAIEKHKKSMSLIELSTTLKCDPSY
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University