Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Arachis ipaensis
Species name
Arachis ipaensis is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Arachis_ipaensis.FLS.fa
Download
>rna-XM_016332754.1
MATKLRLPMVDLSSPDRLSTANSIRQACVDYGFFYLVNHGVDDQLLSSAFQQSAKFFTFPLNYKLSLSRKEYRGYTPLYSENLDPSSHSKGDAKESYYIGNLEDSSSVNLNQWPSQELLPDWRPTMESLIWKLLEAGRKLMSLIALSLNLDDDFFEKTALNKPAAFLRLLHYPGELGSYEELCGASAHSDYGMITLLVTDGVPGLQICKEKFKQPRVWEDVSHVEGAFIVNIGDLMERWTNCIYRSTLHRVIPTGKERYSMAFFLDPPSDCVVECFESCCSESSPPRY
>rna-XM_016351339.2
MELDADRAAQLPKEEKMIYAREPNDMEGYGSDIIYFRDQKLDWTDRIYLKVQPEEQRNFKVWPEKPNDFRTTVIEYTERLKLLNEVILKGMAKSLNLEEYCLLKECGDKDTMLLRFNYYPPCPMADHVLGLKPHADGSTVTYLLQDKEVDGLQVLKDNRWFKVPIISDALLINVGDQVEIMSNGIFRSPIHRVVVNEEKDRLTIAMFFTPDSDKEIGPIEQLVNESRPKSYTPVKNYTDIFFQYYQKGKRAIEASKIPLCEAE
>rna-XM_016311818.2
MEVLRVQRVASESKDASIPAMFVRSETEQPGITTVQGVNLEVPVIDFSNPNQEKVLREVYEASKDWGMFQIVNHEIPSQLITNLQGHKRRRRCMRR*KDLSHWKVMGQSFRRM*MERRVGWTICSMLYGHLHQLTTVSGLKTLLPTGR*MKNTASTYVEW*TS**RVCQ*G*GLKGMS*RSMLVEMT*FTF*RSTITHHAHALILFLVCHSTQTCPILPFLSPTRFKAFKPLEMDIGTMLSMCLMPWLFILVTKWRSLAMGSTRQYYIEQQ*RKTRQECRGQCL*SPKEST*LVLTQSWLTKITLQNTRQRNTKIMPTASLIRPLS
>rna-XM_016315730.2
MAESNSEVEKVVLGNFVQDLILTSQEVQTNYVYTEGGVGFRDALDSGGIPVIDLSLLTSPFELTKLHHALSSWGCFQAINHGMSSSFLDKVREVSKQFFELPKEEKMIYAREPNDMEGYGSDIIYFRDQKLDWTDRIYLKVQPEEQRNFKVWPEKPNDFRTTVIEYTERLKLLNEVILKGMAKSLNLEEYSLLKECGDKDTMLLRFNYYPPCPMADHVLGLKPHADGSTVTYLLQDKEVDGLQVLKDNRWFKVPIISDALLINVGDQVEIMSNGIFRSPIHRVVVNEEKDRLTIAMFFTPDPEKEIGPIEQLVNESRPKSYTPVKNYTDIFFQYYQKGKRAIEASKIQLCEAE
>rna-XM_016314784.2
MDLPVVDLSPYLAASPAQLSPELTALCGEVSRSLRETGALLVKDPRCSVEDNDRFIDMMERYFQSPQDFKQRHERPYLHYQVGVTPEKVEVPRSLVDEEMQEKLKAMPKEYQPHAPVGPDLKWRYFWRIGPRPSNTRFKELNAEPVIPEGFSEWKETMDSWGNKMIAAIEVVAEMAAIGFGLQKDAFTSLMKLGPHLLAPTGSDLQKYGQEGTVLAGYHYDLNFLTIHGKSRFPGLNIWLKNGQKVEVKVPLGCLLIQTGKQIEWLTGGDCIAGMHEVVVTNRTVEAIRTAEEQKRSLWRVSSTLFAHIASDAVLKPLGHFVESPLASKYPPLCAGEYVEQELAVINLKGQK
>rna-XM_021111492.1
MGVERIQTLAFSNQLKELPPQFIRPVNERPENTKAVEGVTVPVISLSQPHHHLLVREVAEAASQWGFFLLTNHGISPWLFKLLQEVGEQFFALPQKEKEAYANDPSNGNFEGYGTKMTKNLEEKVEWVDYFFHIMAPSSKVNYDKWPKNPPSYRNFNLYFLKEYNKEMLRLTNEVLELLSEGLELEKKTLKSSLGDEKIELEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNEVSGLQIWKDNKWVAVDYLQNAIFVHIGDQLEILSNGKYKSVLHRSLVNKECKRMSWAVFVVPPHEVVIGPLPPLFNDHDPPKFSIKTYAEYRHCKFNKLPQ
>rna-XM_016319120.2
MPEAVVDFRAPPPSPVASGRRSSVTNDEVLTEFLESSLRVPDLVLPDKFFPKQKLLETPPKVDFVSLCFHRDEAECDAVLDAMAKIGCFQLMNHGIPRELVVSAAEAAAGVFGVPPVKREALMRSPEKPWGYEEYHGGEEEEEGSEMCEEFVWCREDELKLNMEGISPIGYPNFSKKMEKLMLRIETVAEKILPVILEKIARKIKVDDMDLVHHGHDVGTVCCIYKHCRDNREDQWPNSLKYDVIRMLIRGTDYSHSLGFHVCDGSTEFHVYSKKSWLSFCPEEGAIIITGGDETQRMSDGHYKHVIGRPIFRGKNEDSISMAFLYHTNDAKNDIQTNSGRTISLAQQAVLAIILTLMYHVLIFVTKFLN
MATKLRLPMVDLSSPDRLSTANSIRQACVDYGFFYLVNHGVDDQLLSSAFQQSAKFFTFPLNYKLSLSRKEYRGYTPLYSENLDPSSHSKGDAKESYYIGNLEDSSSVNLNQWPSQELLPDWRPTMESLIWKLLEAGRKLMSLIALSLNLDDDFFEKTALNKPAAFLRLLHYPGELGSYEELCGASAHSDYGMITLLVTDGVPGLQICKEKFKQPRVWEDVSHVEGAFIVNIGDLMERWTNCIYRSTLHRVIPTGKERYSMAFFLDPPSDCVVECFESCCSESSPPRY
>rna-XM_016351339.2
MELDADRAAQLPKEEKMIYAREPNDMEGYGSDIIYFRDQKLDWTDRIYLKVQPEEQRNFKVWPEKPNDFRTTVIEYTERLKLLNEVILKGMAKSLNLEEYCLLKECGDKDTMLLRFNYYPPCPMADHVLGLKPHADGSTVTYLLQDKEVDGLQVLKDNRWFKVPIISDALLINVGDQVEIMSNGIFRSPIHRVVVNEEKDRLTIAMFFTPDSDKEIGPIEQLVNESRPKSYTPVKNYTDIFFQYYQKGKRAIEASKIPLCEAE
>rna-XM_016311818.2
MEVLRVQRVASESKDASIPAMFVRSETEQPGITTVQGVNLEVPVIDFSNPNQEKVLREVYEASKDWGMFQIVNHEIPSQLITNLQGHKRRRRCMRR*KDLSHWKVMGQSFRRM*MERRVGWTICSMLYGHLHQLTTVSGLKTLLPTGR*MKNTASTYVEW*TS**RVCQ*G*GLKGMS*RSMLVEMT*FTF*RSTITHHAHALILFLVCHSTQTCPILPFLSPTRFKAFKPLEMDIGTMLSMCLMPWLFILVTKWRSLAMGSTRQYYIEQQ*RKTRQECRGQCL*SPKEST*LVLTQSWLTKITLQNTRQRNTKIMPTASLIRPLS
>rna-XM_016315730.2
MAESNSEVEKVVLGNFVQDLILTSQEVQTNYVYTEGGVGFRDALDSGGIPVIDLSLLTSPFELTKLHHALSSWGCFQAINHGMSSSFLDKVREVSKQFFELPKEEKMIYAREPNDMEGYGSDIIYFRDQKLDWTDRIYLKVQPEEQRNFKVWPEKPNDFRTTVIEYTERLKLLNEVILKGMAKSLNLEEYSLLKECGDKDTMLLRFNYYPPCPMADHVLGLKPHADGSTVTYLLQDKEVDGLQVLKDNRWFKVPIISDALLINVGDQVEIMSNGIFRSPIHRVVVNEEKDRLTIAMFFTPDPEKEIGPIEQLVNESRPKSYTPVKNYTDIFFQYYQKGKRAIEASKIQLCEAE
>rna-XM_016314784.2
MDLPVVDLSPYLAASPAQLSPELTALCGEVSRSLRETGALLVKDPRCSVEDNDRFIDMMERYFQSPQDFKQRHERPYLHYQVGVTPEKVEVPRSLVDEEMQEKLKAMPKEYQPHAPVGPDLKWRYFWRIGPRPSNTRFKELNAEPVIPEGFSEWKETMDSWGNKMIAAIEVVAEMAAIGFGLQKDAFTSLMKLGPHLLAPTGSDLQKYGQEGTVLAGYHYDLNFLTIHGKSRFPGLNIWLKNGQKVEVKVPLGCLLIQTGKQIEWLTGGDCIAGMHEVVVTNRTVEAIRTAEEQKRSLWRVSSTLFAHIASDAVLKPLGHFVESPLASKYPPLCAGEYVEQELAVINLKGQK
>rna-XM_021111492.1
MGVERIQTLAFSNQLKELPPQFIRPVNERPENTKAVEGVTVPVISLSQPHHHLLVREVAEAASQWGFFLLTNHGISPWLFKLLQEVGEQFFALPQKEKEAYANDPSNGNFEGYGTKMTKNLEEKVEWVDYFFHIMAPSSKVNYDKWPKNPPSYRNFNLYFLKEYNKEMLRLTNEVLELLSEGLELEKKTLKSSLGDEKIELEMKINMYPPCPQPELALGVEPHTDMSALTLLVSNEVSGLQIWKDNKWVAVDYLQNAIFVHIGDQLEILSNGKYKSVLHRSLVNKECKRMSWAVFVVPPHEVVIGPLPPLFNDHDPPKFSIKTYAEYRHCKFNKLPQ
>rna-XM_016319120.2
MPEAVVDFRAPPPSPVASGRRSSVTNDEVLTEFLESSLRVPDLVLPDKFFPKQKLLETPPKVDFVSLCFHRDEAECDAVLDAMAKIGCFQLMNHGIPRELVVSAAEAAAGVFGVPPVKREALMRSPEKPWGYEEYHGGEEEEEGSEMCEEFVWCREDELKLNMEGISPIGYPNFSKKMEKLMLRIETVAEKILPVILEKIARKIKVDDMDLVHHGHDVGTVCCIYKHCRDNREDQWPNSLKYDVIRMLIRGTDYSHSLGFHVCDGSTEFHVYSKKSWLSFCPEEGAIIITGGDETQRMSDGHYKHVIGRPIFRGKNEDSISMAFLYHTNDAKNDIQTNSGRTISLAQQAVLAIILTLMYHVLIFVTKFLN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University