Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Trema orientalis
Species name
Trema orientale is a species of eudicot in the family Cannabaceae (hemp family).
Species info
Gene sequence info
Trema_orientalis.HI4_OMT.fa
Download
>rna-gnl|WGS:JXTC|TorRG33x02_023300.1
MDIIQGQGSSELFKAQSHLYKYVLCYLSSMSLKCAVQLGIADIINSHGRPTTLPELVSALQIHTTKTDFVHRLMRLLTHFGFFTTTKVGHYREEDQEYAYDLTPSSRLLLKDKLPNLSPFVLAVLDPVCVTPFHFLGSWIHREDEVLTPFESAFGVSFRDYAKDNSEYSSMFNEAMASDSGMINLVIKDCKPVFEGLSSLVDVGGGTGKTAGIITEAFPHLTCTVLDLPHVVANLADTGNLKFIAGDMFQHIPPADAWILHDWSDEECLKILKKCKEAIPSNGKGKLIIIDIVLNDNKEDDDHDFTEAKLYFDFMMMVMFTGRERSEKDLKKLLLGAGFSHYKITPMFGLRSLIEVFP
>rna-gnl|WGS:JXTC|TorRG33x02_048110.1
MDCIQGQGSNELFKAQSHLYKYVLCYLSSMSLKCAVQLGIADIINSHGRLTTFPELVFALQIHTTKTGLIHRLMRLLTHLGFFTTTKVGGHYREEDQEDAYDLTPSSRLLLKDKLPNLSPFVLAALDPASVTPCHFLTSWICREGAVRTPFESTTGVNFWDYTNDNSEFNNVFNEAMASDSETINLVVKDCKPVFEGLSSLVDVGGGTGKTAGIITEAFPHLTCTVLDLPHVVANLADTGNLKFIGADMFQHIPPADACVLHDWSDEECLKILKKCKEAIPSNGKGKLIIIDVVLNDNTEDDDHDLTEAKLYFDFLMMVLVTGRERSEKDLKKLFLGARFSHYKITPMFGLRSLIEVFR
>rna-gnl|WGS:JXTC|TorRG33x02_133600.1
MGSICKLTNWVLHDWGDELCVKLLKNCRKAIPEKTGKLIIADIVVEAEGTGPFDDTGVVFDLVLLAHNTGGKERTEKEWKKLLAQAGFPRYKITKIPALTSIIEAYPK
>rna-gnl|WGS:JXTC|TorRG33x02_226010.1
MDITNYLSEVSSAELLEAHSLLWNCSVAHIKSMSLKCAVELGIHDTIHNHGQPMTLSELIASLPIHPSKAQYIYRQMRVLVHSGFFTIQKLDKSTNNRKDQEEEYYYSLTLASRLLLKEGALKTKGFFLSQILPEAMAPWHSLSAWFQNNDATMFETSDGRTFWDYLAQDSRMSDIFNEALSSDSQLIAEVVLRECREVFEGLKSMVDLGGGTGTMAKAIAKAFPHISCTVFDLPHVVADLKGSDNNINFIGGDMFPDPIPSADAILLKLVLHDWKDEDSLNILKRCREAVSIDGKKGKVIIIDMVIGDQNIDKELTEIQLSFDMTMMAFTLGKERNKMEWERLLLGAGFSHYKIHPALGVRSLIEVYP
>rna-gnl|WGS:JXTC|TorRG33x02_294340.1
MDLIQGQGSSELFQAQSHLYKHILSYVNSMSLKCAVQLGIPDTINNHGQPITLPELVSALQIHPTKTGFVHRLMRLLVHSGFFTTTKVSQKQEEEEAYELTPCSRLLLKDKVPCLSPVVRLVLDPAIVAPWHFLEDWFRKEEVQVPFESTHGVTLWDYADKNREFNDLFNEAMASDSGMMNSVVKDCKPVFEGLSSLVDVGGGTGTLCKIITEAFPQLKCTVLDLPHVVVLHDWNEEECLKILKKCRGAIPCNGKGKVIIIEIVINDHKDEQELTEGKLYFDLMMMAVVTGRERSEKEWKKLFLEAGFTRYKITPIFGLRSLIEVFP
>rna-gnl|WGS:JXTC|TorRG33x02_294370.1
MASDSAILHSVVKDYKHVFEGLNSLVDVGGGTGKTSRIITEAFPHLQCTVLPHMVANLPETENLKFVGGDMFRHITPADSVLLEL
>rna-gnl|WGS:JXTC|TorRG33x02_294350.1
MDHIHGPQSNELFQAQSHLYKHIQGFISSMSLKCAVHLGIPDIINNHGRSITLPELVSALQIHPSKTGFVHRLMRLLVHSGFFTATKVSQNQEEEAYDLTPSSRLLLKNKVPCLSAFVLSMLDPAFITPWHSLGKWFQREEALTPFESAHEMNLWEYGKKNSEFNDLFNEAMVSDSGMMNLVVKDCKPVFEGLRSLVDVGGGTGKVCRILNEAFPHLKCTVLELPHVIADLPDTENLKYIGGDMFQAIPQADAILLKLILHGWSDEECLKILKKCRGAIPSNGGKVIVIDIVIIDDKDEHELTEAKLYFDLLMMAVVTGRERSKKDWENLFVESGFTSFKITPIFGLRSLIEVFP
>rna-gnl|WGS:JXTC|TorRG33x02_294410.1
MCKGTYSSTSSWICLLISEIDHIETLDMSQDQDSSELFQAQAHLYKHVLSYMSSMSLKCAVQLGVPDVINNHGQPITLPELVFALQIHPKKTDFVRRIMRLLAHSGFFRTTKLDQNNEGEEEDEEEAYDLTPTSRLLLKNRVPNLSAFVVGLSDPAFVTPAHFLADWLRGDDDQVLTAPFDSTFGLNLWDYANKNPKFNDDFNEAMASDSEMMNLVVKHCKPVFEGLSSVVDVGGGTGKIARIITEECPHLKFTVLDLPHVIANLPETENLKFIEGDMFKHIPPTDAVFLKLILHDWSDEQCLKILKNCREAIPDNAKGKVIIIDIVLNDINKENDDDDPSLSLTADAKLHYDLMMMACLAGKERSEKEWKQLFLGAGFSHYKITPIFGLRSLIEVYP
>rna-gnl|WGS:JXTC|TorRG33x02_294360.1
MDLDHSQDSTAIVDPPLSLNWSPSFRLIHPGKTGSIRRLMRILTHSGFFTATKVDQHDIQEEAYDLTPSSRLLPKDKVPNMSDSSLLD
MDIIQGQGSSELFKAQSHLYKYVLCYLSSMSLKCAVQLGIADIINSHGRPTTLPELVSALQIHTTKTDFVHRLMRLLTHFGFFTTTKVGHYREEDQEYAYDLTPSSRLLLKDKLPNLSPFVLAVLDPVCVTPFHFLGSWIHREDEVLTPFESAFGVSFRDYAKDNSEYSSMFNEAMASDSGMINLVIKDCKPVFEGLSSLVDVGGGTGKTAGIITEAFPHLTCTVLDLPHVVANLADTGNLKFIAGDMFQHIPPADAWILHDWSDEECLKILKKCKEAIPSNGKGKLIIIDIVLNDNKEDDDHDFTEAKLYFDFMMMVMFTGRERSEKDLKKLLLGAGFSHYKITPMFGLRSLIEVFP
>rna-gnl|WGS:JXTC|TorRG33x02_048110.1
MDCIQGQGSNELFKAQSHLYKYVLCYLSSMSLKCAVQLGIADIINSHGRLTTFPELVFALQIHTTKTGLIHRLMRLLTHLGFFTTTKVGGHYREEDQEDAYDLTPSSRLLLKDKLPNLSPFVLAALDPASVTPCHFLTSWICREGAVRTPFESTTGVNFWDYTNDNSEFNNVFNEAMASDSETINLVVKDCKPVFEGLSSLVDVGGGTGKTAGIITEAFPHLTCTVLDLPHVVANLADTGNLKFIGADMFQHIPPADACVLHDWSDEECLKILKKCKEAIPSNGKGKLIIIDVVLNDNTEDDDHDLTEAKLYFDFLMMVLVTGRERSEKDLKKLFLGARFSHYKITPMFGLRSLIEVFR
>rna-gnl|WGS:JXTC|TorRG33x02_133600.1
MGSICKLTNWVLHDWGDELCVKLLKNCRKAIPEKTGKLIIADIVVEAEGTGPFDDTGVVFDLVLLAHNTGGKERTEKEWKKLLAQAGFPRYKITKIPALTSIIEAYPK
>rna-gnl|WGS:JXTC|TorRG33x02_226010.1
MDITNYLSEVSSAELLEAHSLLWNCSVAHIKSMSLKCAVELGIHDTIHNHGQPMTLSELIASLPIHPSKAQYIYRQMRVLVHSGFFTIQKLDKSTNNRKDQEEEYYYSLTLASRLLLKEGALKTKGFFLSQILPEAMAPWHSLSAWFQNNDATMFETSDGRTFWDYLAQDSRMSDIFNEALSSDSQLIAEVVLRECREVFEGLKSMVDLGGGTGTMAKAIAKAFPHISCTVFDLPHVVADLKGSDNNINFIGGDMFPDPIPSADAILLKLVLHDWKDEDSLNILKRCREAVSIDGKKGKVIIIDMVIGDQNIDKELTEIQLSFDMTMMAFTLGKERNKMEWERLLLGAGFSHYKIHPALGVRSLIEVYP
>rna-gnl|WGS:JXTC|TorRG33x02_294340.1
MDLIQGQGSSELFQAQSHLYKHILSYVNSMSLKCAVQLGIPDTINNHGQPITLPELVSALQIHPTKTGFVHRLMRLLVHSGFFTTTKVSQKQEEEEAYELTPCSRLLLKDKVPCLSPVVRLVLDPAIVAPWHFLEDWFRKEEVQVPFESTHGVTLWDYADKNREFNDLFNEAMASDSGMMNSVVKDCKPVFEGLSSLVDVGGGTGTLCKIITEAFPQLKCTVLDLPHVVVLHDWNEEECLKILKKCRGAIPCNGKGKVIIIEIVINDHKDEQELTEGKLYFDLMMMAVVTGRERSEKEWKKLFLEAGFTRYKITPIFGLRSLIEVFP
>rna-gnl|WGS:JXTC|TorRG33x02_294370.1
MASDSAILHSVVKDYKHVFEGLNSLVDVGGGTGKTSRIITEAFPHLQCTVLPHMVANLPETENLKFVGGDMFRHITPADSVLLEL
>rna-gnl|WGS:JXTC|TorRG33x02_294350.1
MDHIHGPQSNELFQAQSHLYKHIQGFISSMSLKCAVHLGIPDIINNHGRSITLPELVSALQIHPSKTGFVHRLMRLLVHSGFFTATKVSQNQEEEAYDLTPSSRLLLKNKVPCLSAFVLSMLDPAFITPWHSLGKWFQREEALTPFESAHEMNLWEYGKKNSEFNDLFNEAMVSDSGMMNLVVKDCKPVFEGLRSLVDVGGGTGKVCRILNEAFPHLKCTVLELPHVIADLPDTENLKYIGGDMFQAIPQADAILLKLILHGWSDEECLKILKKCRGAIPSNGGKVIVIDIVIIDDKDEHELTEAKLYFDLLMMAVVTGRERSKKDWENLFVESGFTSFKITPIFGLRSLIEVFP
>rna-gnl|WGS:JXTC|TorRG33x02_294410.1
MCKGTYSSTSSWICLLISEIDHIETLDMSQDQDSSELFQAQAHLYKHVLSYMSSMSLKCAVQLGVPDVINNHGQPITLPELVFALQIHPKKTDFVRRIMRLLAHSGFFRTTKLDQNNEGEEEDEEEAYDLTPTSRLLLKNRVPNLSAFVVGLSDPAFVTPAHFLADWLRGDDDQVLTAPFDSTFGLNLWDYANKNPKFNDDFNEAMASDSEMMNLVVKHCKPVFEGLSSVVDVGGGTGKIARIITEECPHLKFTVLDLPHVIANLPETENLKFIEGDMFKHIPPTDAVFLKLILHDWSDEQCLKILKNCREAIPDNAKGKVIIIDIVLNDINKENDDDDPSLSLTADAKLHYDLMMMACLAGKERSEKEWKQLFLGAGFSHYKITPIFGLRSLIEVYP
>rna-gnl|WGS:JXTC|TorRG33x02_294360.1
MDLDHSQDSTAIVDPPLSLNWSPSFRLIHPGKTGSIRRLMRILTHSGFFTATKVDQHDIQEEAYDLTPSSRLLPKDKVPNMSDSSLLD
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University