Enzyme

Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.

5AT

Enzyme

Anthocyanin 5-aromatic acyltransferase

Enzyme name

EC:2.3.1.153

Entry

Transferases;Acyltransferases;Transferring groups other than aminoacyl groups

Class

4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase

Sysname

4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [RN:R05163]

Reaction

CoA [CPD:C00010];anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [CPD:C06363]

Product

Species information

Theobroma cacao

Species name

Cacao (Theobroma cacao) is a species of eudicot in the family Malvaceae (mallow family).

Species info

Gene sequence info

Theobroma_cacao.5AT.fa

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>rna-XM_007037839.2
MTMIDIQIVSKETIKPAIPTPHHLRNLKLSFLDQLAPPIYIPIVLFYPAKQNVDLLERSLLLKKSLSKTLTQFYPLAGTMREDFTFECNDEGVEYFETKVPCKLVDVTENPDVNVLNLFLPFEPQQNCIESKKQVPLAIQYNIFKCGGVAIGIRLSHLIADGTSVITFVNAWAAMSREPGEVITPIFEAATHFPPKDISMFRPSIGITKEKIVTKRFVFDKPSIAVLREKASSRDGSQVKTPTRVEAISSFIWSRQMAIAKTKPERAKLYAAVHAVNLRERMAPSLPKHSFGNFWRMAIATFPAEMEQDYHVLVSHMRNAISKIDNNYVKMLQDGDRYLKTMKMVSEQFSKSEVEFCNFTSWCRFPVYEVDFGWGKPAWACSPSRPYKNLVILMSDKGGEGVEAWVNLLEEDMAIFERDPELLSFASSKTGK
>rna-XM_018118843.1
MKILEFTRIKPSPDSPKSAAELSLPLTFFDIFWFKLPPVERLFFYQINNSTPAYFNSVILPKLKHSLSLTLLHYLPLAGNLKWPSTSPKPIILYTPNDGVSLTVAESAADFNLLSSKGIYEAVELHPLTPELITSDDSASTIALQITLFPNMGFCIGITAHHAVLDGKATTLFMKSWAYLCNQGYTEHSPLPPELTPFLDRSVIKDVTGLDLDMLYLNQWLASIGSDSATNNKSLKILPNKGEAPNLVRATFEITREDFKKLRDRALAQLSDSGKELHLSTFVLTLAYVTRCIVKARGGEDDRNVGVGFTIDCRPRLNPPVPENYFGNCNTITGDLFKARDFLDENGFGFSVHKVSGMVKVLMEKGPLVGIENKLSTVLNFSEEIAKSMQIISVAGSPRFEVYGSDFGWGKPLKVVVVSIDKNEAISMAESRDGSRGVEVGLALKKHEMEFFSSLFLKDV
>rna-XM_007033803.2
MAVLPDTLKVLEDTHVFPPPGSVPTTSLPLTFFDIHWLGSSPMQRLLFYDFPYPTFYFTQSTLPNLKRSLSLTLQHFFPLAGNLVFPPPPQKPYILYKEGDSVQFIAKESTADFSHLKGDHARHVQEFQALLPKLKPASTYSHSTTSTACMQKPLMAIQVTLFPKAGICVGATFNHVAADGKAFTHFMKSWASAHRSQGDLMTCLNKSLPDYNKDLIKDPLGLASIFMKDKWNWEDLDSIPYDKFRVTFVIKRSQVELLKNWVTRKCMEENGSETLRTSTFVVTCAYMWVCLVQLRESGTHDLSSTDSYNMLCHFIFLADCRDRLKLPATYFGNCLEPRFAAAKKSELLGGKGILVAAKAIGREVMELEKGALREAEKWLSRAEEIFKFGRHVCIAASPKLRVYETDFGWGRPRKTEVAHIGSFGSISMAESREEEGGVEFGLALAQDELDSFNAVFEQGLLKLQ
>rna-XM_018119022.1
MSNSNFSLLSGNEIHKALELHSLEPQLMMTDNIATIIALQITLFPSQGFCVGITTHHAILDGKSTTMFVKSWVYLCKKGNTKNSSLPPKLTLFYDRSVIKDPSGLDLVYLNQWLAFTSSDSDPNRRSLIILQNIKDVSDDLLRPTFDLTHEHIKSLREKVLSKLDKAKPLHLSSFVLTFAYVSTCLIKARGGESDKMVNLGFATDARSHLNPPIPKNYFGNCVLGLLASAKAGNFMDENGLPLLRSWQAIWLKSWRRKGFLNRQKRS
>rna-XM_007028015.2
MGTICVEPPSLLQDLKITIQNVSLVFPSQQTERKSMFLSNIDKVLNFPVETVHFFPSHKDFPPHLVAEKLKSTLARLLVPYDFLAGRLRSNPETGRLEIDCNAAGIGFAVASSEYTMDSLGELVYPNPAFGQLILKRMDNLEQDDQPLCIVQVTSFRCGGFAMGMFTNHVTFDGLSFKIFLDNLGALAADKPLAVIPCNDRELLAARSPPCVSFPHLELAKFLDSNVNHPVFDATSEALDFKVFRLTSGNIADLKEKAKASGGTHKDARITGFNVVTALVWLCKALSWDEETTQERNNNADRESTLLYAVNIRPRLIPPLPRSYTGNAVLTAYATAKCKEIEEWPFSRLVEMVSEGAKRMTDEYARSAIDWGEIYQGFPNGEFLVSSWWKLGFDEVDYPWGRPRYSCPVVFHRKDIILLFPDIDENNGVNVLVSLPSKEMSKFQSLFLNYLA
>rna-XM_007028164.2
MEKMKVEVTSRKTIKPSIPTPHHLRTFNLSLLDQDVPAIHYGSVVFFYPSDNTAPNVRSSISQKSQSLKNSLSRILLHFYPLAGQVKDAVTIECNDQGACFIEARTGCQLNDFLTHPDPGFLKELVPSTDPKAIRSTLACILLVQLTSFTCGGTAVAVCVSHKFADASSLCTFIQSWTAMSSREYGRVVVPKLVGASLLPPIHKLITSTPPPTTRNCTTKRFVFHKSQIANLKAKVAATMGKQQRPANVEIVLAIVLRCASAASQSKYGSSRQSALLNVVNLRKRMVPPLPGNTIGNLIWTYPVLFQESDVQLHQIVAKMKDAFTNVCNDKLKTIKSKKGHTEVQESRKQIAQLLNGKLVDTNTYTCTNLCSYPLHEMDFGWGKPLWVTSPSNFKNLIVLLDSKWGDIEAWVTLDELEMATFERDQELLAVASSNPSALVSYSRM
>rna-XM_018121169.1
MEVRITSRETVQPSSQEVHLLKPFKLSFLDQLIPAFYVPLIFFYTQSSNSHFDSAQILARLKESLSKTLNQFYPLSGRTIDNFCIDYYREGVPYIEARVNGYLSDYLQSKELEKLNHLIPCEPFCYFSDPAVIPQLAVQVNIFDCGGIALAMCCSHKIIDASTVSAFLRSWAAFSRGSNGEIPHPDLLEASSRLFPPMDSMPPNFLSSTRTLWFKEERYKTRRFVFDANAIATLMFKAKSKSLENPSRVEALTAFIWKYAMLACASASGILKPSVLCQAVNLRRKMKPRLPDYSIGNLIWFAAFAYNPADKDIELHHLAYLLREAVENYNTDYLQSLQGDEGFKVISEKANQVAEFATKGNAQVYGFSSWLNFGMHQVDFGWGKPSWIGIPGVVSPAFTNLTFLKEIGQEKAVEAWVTLDEKKMTIFEHDHEFLAFASPNPHYDKVKI
>rna-XM_007026713.2
MPSSSATLISKCTIFPDQKSNVKTLKLSVSDIPMLSCQYIQKGVLLTAPPISFDDLVVFLKQSISTTLSHFPPLAGRLMTDPDGHVHITCNDAGIEFLVVKSPKVSIHDILCPGDVPNCVKEFFTFDKTLSYSGHFKPLAAVQMTELVDGVFIGSTVNHAVTDGTSFWHFFNTFAEMTKGASKISKTPDFSRNTVFNSQAVLKFPPGGPTVTFAGNEPLRERIFHFTREAILKLKYRANYGRFLTKQTNSEVLGKLCNDSWKSVNGETNGLSNGKVKNSNDEISSFQSLCAQLWRSVTRARKLEITKTTTFRMAVNCRHRLDPKLEPYYFGNAIQSIPTFAPAGELLAKDLGWSADLLHKNVVAHDDGTVRGGVADWEKQPRLFPLGNSDGASITMGSSPRFPMYDNDFGWGRPLAVRSGRANKFDGKISAFPGREGNGSVDLEVVLAPDTMAGIENDAEFMQYVSAIV
>rna-XM_007025391.2
MKVQVTETALIRPSTVPFADDHTLALSHLDNDHSLNVTFRYLRAYVNSTNTDRNPFQVISPAIATALHHYYPLAGSLRRASNGRYELLCQVDQSLPLVNASADCTLESVNYLDDPDMNSVEQLVPDPSPEETLLNPCTLQVTVFKCGGFTLGAAIHNALCDGLGATQFFCMAADLARGVDQIKFQPVWDRAALLGPRNPPRPEGPVREFLSLEKGFNPYKQDVGHVVRACFYVEDECLDQLKSLLFEQCGLGLTTFEILGAYIWRAKVKASKNPGDEIVKYSYLMNIRKIVKPPLPPGYWGNGCVAMYAKVSAKDLTEQPLWKTAELIKKSKSNASDEYVRSFIDLQELHYEEGITAGKGVSGFTDWRHLGHSAVDFGWGGPVTVLPLSTNFLGSMEPCFFLPYSSSSTGKNKGFKVLVSLCETAMPAFKEEMEKFSRKEFDI
>rna-XM_007013730.2
MAEGTVTHIGKRSVVSSRWHAEPGKFCHLSVLDRLMEQNQVKIVYYYPSRGDHAEPGEWIKKLRDSLSCVLTNFPILTGRLHKNQEGHWMVKCNDGGVRMVEAKVKGSVEDWLKSVDREKELKLLYWEEMFHKPYHWPAVYVQVTEFEKGGLAIGLSCCHLLADPICATMFSKAWADTTLSGIMSTPPLFHSLPLTKADYSKIPNDESCTDLINYYKYSIERLTPLTTNQKYATVTLSFTDSMVRDCIAMAQATGASGKSGSPAFEALAALFWVCLSKVKATKGRLFNMSLCLDMRNCLGLDPGFFGNCMVCNKVHLESAGETMLPEATKAISEVVKKMNAEGIKDLIQWLKCNDNQTVPLMNDYDLICVNLEGVNPYLATFKDQLEPLQVSYYVEPMVGPGQVLISRSAPGEGPLSRVVMATLPEDEVAKLCEDDLIQRFSPAVLMGTNKN
>rna-XM_018129391.1
MPSSPLVLVSKSTVFPDRKSNLGELKLSVSDLPMLSCHYIQKGCLFTLPPVPIDSLVSLLKQSLSKTLSFFSPLAGRLHTDPNGYIYVSCNDAGVEFHHSKCATRFVRDVIGPIHVPELVKEFFCFDKTVSYQGHYKPIMAVQVTELADGFFIGCSVNHAIVDGTSFWNFFNTFAEICRKISNASDDRSVEKIARQPDFSRDSALISSAILRVPEGGPRVTFNENEPLRERIFSFSREAILELKAKVNNTKEDLLVNGNFNAVEILGKQSNDKYHNDNGKNLTGIFEKWLFKTTAISDSVEISSFQSLCALLWRAVTRARKLPYSKTTTFRMAVNCRHRLNPKLDPLYFGNAIQSIPTYATAGEVTSRGLRWCAEQLNESVKAHDDETVRRFIGNWEKDPRCFPLGNFDGASMTMGSSPRFPMYDNDFGWGRPLAVRSGGANKFDGKISAFPGREGNGSVDLEVVLAPETMAGIESDHEFMQFVTN
>rna-XM_007009022.2
MPSSAPCIALVSKSNVFPDQKSKLGELKLSVSDLPMLSCHYIQKGCLFTRSPIPIDSLVSLLKQSLSKTLSFFPPLAGRLHTDPNGYIYVTCNDGGVEFYHATCKGLSIRDVIGPLDVPEFVKKFFCFDKTVSYQGHFKPIMAVQVTELGDGVFIGCSVNHAVTDGTSFWNFFNTFAELCKKISSNNQNFEKIARQPDFSRNSVLVSPAILKVPEGGPKVTFNEKERLRERIFSFSREAILELKARVNNNEEELLVNGNFNAVEILGKQSNDKYHNEKSKSRLFRTAPVSNTAEISSFQSLCALLWRAVTRARKLPSFQKTTFRMAVNCRHRLNPKLDPLYFGNAIQSIPTYASAGDVTSRELRWCAEQLNQSVRSHDDETVRRFVKEWEKDPRCFPLGNFDGASMTMGSSPRFPMYDNDFGWGRPLVVRSGGANKFDGKISAFPGREGNGSVDLEVVLAPETMAGIESDHELMQYVTN

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University