Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Theobroma cacao
Species name
Cacao (Theobroma cacao) is a species of eudicot in the family Malvaceae (mallow family).
Species info
Gene sequence info
Theobroma_cacao.ANAT.fa
Download
>rna-XM_018123207.1
MAKDLRVKILEHCQVSPPPDSVNPQTSLIPLTFFDVPWLFFPPTQPLFFYPYPYSTSHFLSATLPTLKQSLSLTLQHFFPFAGHLVLDSKSNRPKIVYNEKHSFVSLTFAESNADFHYISGDRPRDVEEFHPLVPSLERSLQSSDDENEALPLLAVKVTVFPNSGLCIGLAYHHVAADGRTFDNFIKTWASFHKDSSFLITAMPSYDRTLVIDKHGLEAIFLKEWRKKKSAEHEMVIVTKSKAEFSNKVRATFVMGLTEMEKIKRWIVTECKKKNLREPVHLSPYVVTCAFVWGCLMKAACNEGVADKYPNDYPSYLGFNAGGLSRFDCRVPSTYFGNCVGFGRCEATRGDLMGENGIILAAKAIGDTVKKLDKALLGEAERWISDWEVFYRSEPHMMVVGSPKVHLYETDFGWGRPNKIEEISIDQCGAISLTESRCERGGIEVGLALPKPQMDTFTSLFDQGLKAKM
>rna-XM_007043389.2
MAGPCTIKVLDHSNASPPPGSVPDTSLPLTFFDVPWLVCRPMQRLFFYEFPYSTLYFTQTILPSLRTSLSHALQTFYPFAAKLICPPSPHKPHILYTDGDCVSFTVAESSADFDRTVAYLARDVKELHPFVPQLQPSRVLNNTRVLPLLALQVTVFPNSGICIGATFCHVAADGRSFNHFMKAWASILRSGGNSAACLEKSVPFLERNVIREKDSRGLESILMTEWWSWASDWEEDKVPAHDILANKVRATFVMGRGDIDRLKRCVLIRCLKQNESEQLRASTFVVSCAFVWTSLIKAQELCGRGKHEDDEFHYFCFVADCRNRPEFAIPETYLGNCLSICFVRMKRRELLGENGVVAATKAIGKRVWEFEKGALREAEKWLTNWKEISETGHLVTISGSPKLRAYETDFGWGKPKKTEVVQIDVSGAIYLGDSRDEEGGIEIGLALNKDEMEAFHGFFEQGLKTT
>rna-XM_007042615.2
MAQANEKLQVLDCSHVSPPPASVPTTSLRLTFFDLPWFAFPNIQRLFFYEFPYPTLYFTDTVLPLLKHSLSLTLQHFFPYAAKFMCPPPPGKPYLRYEDGDFVTFTVVESSADFNHVIANYPRDVKLLRPFAPQLPTVPVAEDGTRVLPVIAFQVTLFPNSGICIGSNYCHVVGDGKTFMHFMRSWSSVHRAGGGDLTCLEKSIPSWNKDVIKDPDGIESLHLKIYWNWVASNNENSGPTHVAAADKVRATFLLGRAHVKKLKHLVTEAKSEQLHISTFVVTCAFIWVCLIKSQESSTRNSSDADDNKFYYFQFPFDCRNRLEFPVPETYFGNCLKPSIAEVQKSELIGENGILLAAKAIGNKIKEIENRGFRDVESWVATLMERTRLGRFVSVSGSPKFYVYDVNFGWGRPRKVELVHIDSNETISLAECRDEQGGFEVGVASNKKQMDEFSAIFVQSLKLL
>rna-XM_007043397.2
MAQTGTIKVLDSSHVSPPPSSVPTTSLPLTHFDLPWFPCYIERLFFYKFSHPTLHFMETTLPMLKCSLSLTLQHFFPYAANIMCPQPPGKPYIHYIDGDFVTFTVAESAADFNHVKANYPRDIKLLRPFVPQLPPARVAEDGIRVCPITAFQVTVFPNSGICIGSTYWHVVGDGKSFMHFMRSWTAVCRSGGDLTCLENSLPLINKDVIKDPGGIELVRLKNYWHWVSFSNENSGPTHAIAEDKVRATFVLGRAHAERLKHLVTGQCRDGVESEQLHISTFVVTCAFIWVCLIKSKDSATNNLSRDDDDKFYFLLFPFDCRNRLEFPVPPTYFGNCLRPGVVDVTKSELIGENGILLASKVIGNKIKEMERSGLRGAEHWISSLVERMKSRRLTAVAGSPKFHVYDTDFGWGRPCKVELTHIDYDGAISLAECKDEPGGIEVGLALNKNQMDEFITIFEQSLKLL
>rna-XM_007043396.2
MDSPYAAKILDNIHVSAPAGSVPTTTLPLTYFDIPWFPCAPMQSLFFYEYPHPTSHFMQTTLPTLKQSLSLTLQHFFPLAAKLVCPPPPLEPNILYTDGDSVPLTIAESAADVNHIKANYARDIKLLHPFVSLLPPSTMSSHGIRVLPIMALKVTVFPNAAICIGMAHYHVAADGSAFMHFVRSWSSVCKSKGNSTCLDYVSQRPSHSRDAIKDPAGCNDVALKGYWNWVSSWRENLGPTKEMPMDKVRASFALTRAQIEKLKQWLTTQCNGNDLEGLHISTFVVACSMMWICMVKSQESEISANCHDDDKFYYFLFAVDCRSRIEFPLPVTYFGNCLAPGIVRAKKGELMGESGIVTAAKAIARKVKEMESGALKEVEKGPSNLEEMLASGHYLGVSGSPKLGIYGMDFGWGRPTKVEFVHLDDGGSLSLAECRDEPGGIEVSLVLSKDQMDSFTAHFGESLRKAF
>rna-XM_007033805.2
MAQHCFIKFLEASHVSPPPGSVPTTSLPLTFLDTLWISCCHIQRLFFYKFPYPTSHFLHNALPNLKTSLSLTLQHFFPFAGNLKLPPLPQRPYIYYADGDSLPFVVIESTADFKHLITGNRPRHAQDLEDLVPNLPPPSMSSDGDNICKQQPLMAIQVTVFPNAGISIGITFCHVAADGRAFGHFTKSWASICRSLGDSTSVRNIISPPNYSRDLIQDPRGIWTIFLKELYDWETLERMTPTDNVRITPVINKSHVEMLKTWIARKYVEETEKEPPRLSTFVVTTAFMWVCLVKLKQSNKTPDQRLRNDDAICVFTFLADCRYRLRLPTNYFGNCLEPCVATAKRSELIGENGMVVAAKAIGREILEFEKEPLRGAATWISSGKEVLRKCEHYITLAGSPKLRVYETDFGWGRPAKSEVVHLGSQGSIAIAESSDEESGVEFGVPLAPDEFDKFNAIFEQGLLNLCK
>rna-XM_007033803.2
MAVLPDTLKVLEDTHVFPPPGSVPTTSLPLTFFDIHWLGSSPMQRLLFYDFPYPTFYFTQSTLPNLKRSLSLTLQHFFPLAGNLVFPPPPQKPYILYKEGDSVQFIAKESTADFSHLKGDHARHVQEFQALLPKLKPASTYSHSTTSTACMQKPLMAIQVTLFPKAGICVGATFNHVAADGKAFTHFMKSWASAHRSQGDLMTCLNKSLPDYNKDLIKDPLGLASIFMKDKWNWEDLDSIPYDKFRVTFVIKRSQVELLKNWVTRKCMEENGSETLRTSTFVVTCAYMWVCLVQLRESGTHDLSSTDSYNMLCHFIFLADCRDRLKLPATYFGNCLEPRFAAAKKSELLGGKGILVAAKAIGREVMELEKGALREAEKWLSRAEEIFKFGRHVCIAASPKLRVYETDFGWGRPRKTEVAHIGSFGSISMAESREEEGGVEFGLALAQDELDSFNAVFEQGLLKLQ
MAKDLRVKILEHCQVSPPPDSVNPQTSLIPLTFFDVPWLFFPPTQPLFFYPYPYSTSHFLSATLPTLKQSLSLTLQHFFPFAGHLVLDSKSNRPKIVYNEKHSFVSLTFAESNADFHYISGDRPRDVEEFHPLVPSLERSLQSSDDENEALPLLAVKVTVFPNSGLCIGLAYHHVAADGRTFDNFIKTWASFHKDSSFLITAMPSYDRTLVIDKHGLEAIFLKEWRKKKSAEHEMVIVTKSKAEFSNKVRATFVMGLTEMEKIKRWIVTECKKKNLREPVHLSPYVVTCAFVWGCLMKAACNEGVADKYPNDYPSYLGFNAGGLSRFDCRVPSTYFGNCVGFGRCEATRGDLMGENGIILAAKAIGDTVKKLDKALLGEAERWISDWEVFYRSEPHMMVVGSPKVHLYETDFGWGRPNKIEEISIDQCGAISLTESRCERGGIEVGLALPKPQMDTFTSLFDQGLKAKM
>rna-XM_007043389.2
MAGPCTIKVLDHSNASPPPGSVPDTSLPLTFFDVPWLVCRPMQRLFFYEFPYSTLYFTQTILPSLRTSLSHALQTFYPFAAKLICPPSPHKPHILYTDGDCVSFTVAESSADFDRTVAYLARDVKELHPFVPQLQPSRVLNNTRVLPLLALQVTVFPNSGICIGATFCHVAADGRSFNHFMKAWASILRSGGNSAACLEKSVPFLERNVIREKDSRGLESILMTEWWSWASDWEEDKVPAHDILANKVRATFVMGRGDIDRLKRCVLIRCLKQNESEQLRASTFVVSCAFVWTSLIKAQELCGRGKHEDDEFHYFCFVADCRNRPEFAIPETYLGNCLSICFVRMKRRELLGENGVVAATKAIGKRVWEFEKGALREAEKWLTNWKEISETGHLVTISGSPKLRAYETDFGWGKPKKTEVVQIDVSGAIYLGDSRDEEGGIEIGLALNKDEMEAFHGFFEQGLKTT
>rna-XM_007042615.2
MAQANEKLQVLDCSHVSPPPASVPTTSLRLTFFDLPWFAFPNIQRLFFYEFPYPTLYFTDTVLPLLKHSLSLTLQHFFPYAAKFMCPPPPGKPYLRYEDGDFVTFTVVESSADFNHVIANYPRDVKLLRPFAPQLPTVPVAEDGTRVLPVIAFQVTLFPNSGICIGSNYCHVVGDGKTFMHFMRSWSSVHRAGGGDLTCLEKSIPSWNKDVIKDPDGIESLHLKIYWNWVASNNENSGPTHVAAADKVRATFLLGRAHVKKLKHLVTEAKSEQLHISTFVVTCAFIWVCLIKSQESSTRNSSDADDNKFYYFQFPFDCRNRLEFPVPETYFGNCLKPSIAEVQKSELIGENGILLAAKAIGNKIKEIENRGFRDVESWVATLMERTRLGRFVSVSGSPKFYVYDVNFGWGRPRKVELVHIDSNETISLAECRDEQGGFEVGVASNKKQMDEFSAIFVQSLKLL
>rna-XM_007043397.2
MAQTGTIKVLDSSHVSPPPSSVPTTSLPLTHFDLPWFPCYIERLFFYKFSHPTLHFMETTLPMLKCSLSLTLQHFFPYAANIMCPQPPGKPYIHYIDGDFVTFTVAESAADFNHVKANYPRDIKLLRPFVPQLPPARVAEDGIRVCPITAFQVTVFPNSGICIGSTYWHVVGDGKSFMHFMRSWTAVCRSGGDLTCLENSLPLINKDVIKDPGGIELVRLKNYWHWVSFSNENSGPTHAIAEDKVRATFVLGRAHAERLKHLVTGQCRDGVESEQLHISTFVVTCAFIWVCLIKSKDSATNNLSRDDDDKFYFLLFPFDCRNRLEFPVPPTYFGNCLRPGVVDVTKSELIGENGILLASKVIGNKIKEMERSGLRGAEHWISSLVERMKSRRLTAVAGSPKFHVYDTDFGWGRPCKVELTHIDYDGAISLAECKDEPGGIEVGLALNKNQMDEFITIFEQSLKLL
>rna-XM_007043396.2
MDSPYAAKILDNIHVSAPAGSVPTTTLPLTYFDIPWFPCAPMQSLFFYEYPHPTSHFMQTTLPTLKQSLSLTLQHFFPLAAKLVCPPPPLEPNILYTDGDSVPLTIAESAADVNHIKANYARDIKLLHPFVSLLPPSTMSSHGIRVLPIMALKVTVFPNAAICIGMAHYHVAADGSAFMHFVRSWSSVCKSKGNSTCLDYVSQRPSHSRDAIKDPAGCNDVALKGYWNWVSSWRENLGPTKEMPMDKVRASFALTRAQIEKLKQWLTTQCNGNDLEGLHISTFVVACSMMWICMVKSQESEISANCHDDDKFYYFLFAVDCRSRIEFPLPVTYFGNCLAPGIVRAKKGELMGESGIVTAAKAIARKVKEMESGALKEVEKGPSNLEEMLASGHYLGVSGSPKLGIYGMDFGWGRPTKVEFVHLDDGGSLSLAECRDEPGGIEVSLVLSKDQMDSFTAHFGESLRKAF
>rna-XM_007033805.2
MAQHCFIKFLEASHVSPPPGSVPTTSLPLTFLDTLWISCCHIQRLFFYKFPYPTSHFLHNALPNLKTSLSLTLQHFFPFAGNLKLPPLPQRPYIYYADGDSLPFVVIESTADFKHLITGNRPRHAQDLEDLVPNLPPPSMSSDGDNICKQQPLMAIQVTVFPNAGISIGITFCHVAADGRAFGHFTKSWASICRSLGDSTSVRNIISPPNYSRDLIQDPRGIWTIFLKELYDWETLERMTPTDNVRITPVINKSHVEMLKTWIARKYVEETEKEPPRLSTFVVTTAFMWVCLVKLKQSNKTPDQRLRNDDAICVFTFLADCRYRLRLPTNYFGNCLEPCVATAKRSELIGENGMVVAAKAIGREILEFEKEPLRGAATWISSGKEVLRKCEHYITLAGSPKLRVYETDFGWGRPAKSEVVHLGSQGSIAIAESSDEESGVEFGVPLAPDEFDKFNAIFEQGLLNLCK
>rna-XM_007033803.2
MAVLPDTLKVLEDTHVFPPPGSVPTTSLPLTFFDIHWLGSSPMQRLLFYDFPYPTFYFTQSTLPNLKRSLSLTLQHFFPLAGNLVFPPPPQKPYILYKEGDSVQFIAKESTADFSHLKGDHARHVQEFQALLPKLKPASTYSHSTTSTACMQKPLMAIQVTLFPKAGICVGATFNHVAADGKAFTHFMKSWASAHRSQGDLMTCLNKSLPDYNKDLIKDPLGLASIFMKDKWNWEDLDSIPYDKFRVTFVIKRSQVELLKNWVTRKCMEENGSETLRTSTFVVTCAYMWVCLVQLRESGTHDLSSTDSYNMLCHFIFLADCRDRLKLPATYFGNCLEPRFAAAKKSELLGGKGILVAAKAIGREVMELEKGALREAEKWLSRAEEIFKFGRHVCIAASPKLRVYETDFGWGRPRKTEVAHIGSFGSISMAESREEEGGVEFGLALAQDELDSFNAVFEQGLLKLQ
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University