Enzyme
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
DFR
Enzyme
Dihydroflavonol 4-reductase
Enzyme name
EC:1.1.1.219
Entry
Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Sysname
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Theobroma cacao
Species name
Cacao (Theobroma cacao) is a species of eudicot in the family Malvaceae (mallow family).
Species info
Gene sequence info
Theobroma_cacao.DFR.fa
Download
>rna-XM_018126814.1
MEKETKVCVTGAAGYLGSSLVKKLLEKGHIVHATARNLGDPNKIGLLKSLPNAETRLRLYEADIYNPSQFQHAIEGCRFVVHMATPLQHEPQNHQFKDTSEAAVAGVKSIVESCIRSGSVKRLIYTASVVAASALKEDGEGFKDQLDESCWTPKDVALPYTNELLMGYVSSKAASEKELLRYDSEIEIVSLTCGLVGGDTLHSSMPESTGVLISQATKNKRRYNTLRFLEELLGKVPILHIEDVCDAHIFCMETPSIRGRLLCASAYLSSADIANHCRKIYPDIEIPDEFVLNSGREILWGSTKLKEIGFEYKCDVKTILEDSINCGLKLGEFSIF
>rna-XM_018129818.1
MEGQTAARVCVTGAAGFIGSWLVMRLLQRGYTVKATVRDPANLNKVKHLLELPKADENLILWKADLMEEGSFDEAVRGCSGVFHVATPKDFESQDPENEVIKLTVDGALSILKSCANAKTVERLLFVSSGGTVAMQERKLVQYDETCWSDVDFIRAKKMTGWMYFESKTLAEKAAWGAAQENDIDFISVKPTLVVGPFIIPSMPPSLITALSLITRNEGHYSIIKQCQYVHLDDLCNALVFLYENPEAHGRYICSSHDATIFDLAEMLRQKYPGYDIPIQFRGVEEHLEVISFSSKTLTDLGFQFKYGFENMYTGAIKTCIEKGLIPRCLNDNDHAAVGKIHNIMVLDVGFTSVAINLLPVCLKEA
>rna-XM_007051535.2
MEKETKVCVTGAASFLGSWLVKKLLEKGYTVHATLRNLGDPYKVGLLSSLPNAGTKLRLYEADVYNLGLFKQAIEGCEYVVHMATPLLHQPLSSQFKNTSEAAVAGVKSIMELCIRSGTVKRLIYTASVTAASPIKENGKGFKDRMDESCWTLQEIALPYSNELLVGYTCSKTLSEKELLRYNGELEVVSLVCGLVGGKTLLSSMPESMGVLISQAAKNKRRYRTLRFLEELLGRVPILHIEDVCNALIFCLEKPSIKGRFLCASAYLSSADIASHSRKLYPHIEINEEFVENLGREISWGSAKLEEVGFGYKCDVKMILEDSINCGLRLGECGLHFPATR
>rna-XM_007021042.2
MEDNLGAPAKGKSVVAAPTYCVTGATGYIGSWLVKLLLEKGCKVHATARNPEKALHLLSLWSGSDRLRLFKADLQEEGSFDEAVKGCNGVFHVAASMEFGVNVNENIEGYVRSNIIDPAIKGTENLLKACSKSKSVKRVVFTSSISTITAKDSNGNWRPVVDESCQNTSDHVLNAKAGGWVYALSKLLTEEAAFTFANENGIDLVSLITTTVAGPFLTTTIPSSIQVLLSPFTGDPKYFSILSAVNARMGSVALVHIEDICGAHIFLMEQANAEGRYICCVCSSPLSELIDLLAQKHPCSNLQRLGAEGKGTKPPEISSKKLRDLGFTYKHNIEDIIHQTVTACVDYGFLPPTRR
>rna-XM_007017524.2
MGSEPQSVCVTGASGFIGSWLVMRLLENGYAVRATVRDPDNVKKVKHLLELPKADSHLTLWKADLSEEGSFDAAIQGCTGVFHVATPMDFESKDPENEVIKPTINGVLDIMKACVKAKTVKKLVFTSSAGTVNVEERQKPVYDESNWSDLEFVYAKKMTGWMYFVSKTLAEQAAWKFAKENNLDFITIIPTLVIGPFLMSSMPPSLITGLSPITGNEAHYTIIRQGQFVHLDDLCMAHIFLFENPKAEGRYICSSHEATIVELAKMLREKYPEYNIPTKFKDADENLANVVFSSKKLLDLGFQFKYSLEDMFTGAVETCREKGLIPAPIKKDAVDVKGCTTPVPV
MEKETKVCVTGAAGYLGSSLVKKLLEKGHIVHATARNLGDPNKIGLLKSLPNAETRLRLYEADIYNPSQFQHAIEGCRFVVHMATPLQHEPQNHQFKDTSEAAVAGVKSIVESCIRSGSVKRLIYTASVVAASALKEDGEGFKDQLDESCWTPKDVALPYTNELLMGYVSSKAASEKELLRYDSEIEIVSLTCGLVGGDTLHSSMPESTGVLISQATKNKRRYNTLRFLEELLGKVPILHIEDVCDAHIFCMETPSIRGRLLCASAYLSSADIANHCRKIYPDIEIPDEFVLNSGREILWGSTKLKEIGFEYKCDVKTILEDSINCGLKLGEFSIF
>rna-XM_018129818.1
MEGQTAARVCVTGAAGFIGSWLVMRLLQRGYTVKATVRDPANLNKVKHLLELPKADENLILWKADLMEEGSFDEAVRGCSGVFHVATPKDFESQDPENEVIKLTVDGALSILKSCANAKTVERLLFVSSGGTVAMQERKLVQYDETCWSDVDFIRAKKMTGWMYFESKTLAEKAAWGAAQENDIDFISVKPTLVVGPFIIPSMPPSLITALSLITRNEGHYSIIKQCQYVHLDDLCNALVFLYENPEAHGRYICSSHDATIFDLAEMLRQKYPGYDIPIQFRGVEEHLEVISFSSKTLTDLGFQFKYGFENMYTGAIKTCIEKGLIPRCLNDNDHAAVGKIHNIMVLDVGFTSVAINLLPVCLKEA
>rna-XM_007051535.2
MEKETKVCVTGAASFLGSWLVKKLLEKGYTVHATLRNLGDPYKVGLLSSLPNAGTKLRLYEADVYNLGLFKQAIEGCEYVVHMATPLLHQPLSSQFKNTSEAAVAGVKSIMELCIRSGTVKRLIYTASVTAASPIKENGKGFKDRMDESCWTLQEIALPYSNELLVGYTCSKTLSEKELLRYNGELEVVSLVCGLVGGKTLLSSMPESMGVLISQAAKNKRRYRTLRFLEELLGRVPILHIEDVCNALIFCLEKPSIKGRFLCASAYLSSADIASHSRKLYPHIEINEEFVENLGREISWGSAKLEEVGFGYKCDVKMILEDSINCGLRLGECGLHFPATR
>rna-XM_007021042.2
MEDNLGAPAKGKSVVAAPTYCVTGATGYIGSWLVKLLLEKGCKVHATARNPEKALHLLSLWSGSDRLRLFKADLQEEGSFDEAVKGCNGVFHVAASMEFGVNVNENIEGYVRSNIIDPAIKGTENLLKACSKSKSVKRVVFTSSISTITAKDSNGNWRPVVDESCQNTSDHVLNAKAGGWVYALSKLLTEEAAFTFANENGIDLVSLITTTVAGPFLTTTIPSSIQVLLSPFTGDPKYFSILSAVNARMGSVALVHIEDICGAHIFLMEQANAEGRYICCVCSSPLSELIDLLAQKHPCSNLQRLGAEGKGTKPPEISSKKLRDLGFTYKHNIEDIIHQTVTACVDYGFLPPTRR
>rna-XM_007017524.2
MGSEPQSVCVTGASGFIGSWLVMRLLENGYAVRATVRDPDNVKKVKHLLELPKADSHLTLWKADLSEEGSFDAAIQGCTGVFHVATPMDFESKDPENEVIKPTINGVLDIMKACVKAKTVKKLVFTSSAGTVNVEERQKPVYDESNWSDLEFVYAKKMTGWMYFVSKTLAEQAAWKFAKENNLDFITIIPTLVIGPFLMSSMPPSLITGLSPITGNEAHYTIIRQGQFVHLDDLCMAHIFLFENPKAEGRYICSSHEATIVELAKMLREKYPEYNIPTKFKDADENLANVVFSSKKLLDLGFQFKYSLEDMFTGAVETCREKGLIPAPIKKDAVDVKGCTTPVPV
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University