Enzyme
Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.
5AT
Enzyme
Anthocyanin 5-aromatic acyltransferase
Enzyme name
EC:2.3.1.153
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [RN:R05163]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [CPD:C06363]
Product
Species information
Thellungiella halophila
Species name
Eutrema halophilum is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Thellungiella_halophila.5AT.fa
Download
>PAC:20194671
MSAKEETIMEEERVRFICKRTVVSTKQVEPGQLYRLSVLDHVMEPNHIRLVYYYRCSKTREPGEITKKLRESLAYTLNCYPIITGRLVKEIDGTDEKNELNRQWKVKSNDSGMRMVEARATGTVEEWLRNVNREEELKLVHWEDIYHDLPYYWSTFYVQVTEFESGGLAIGLSCSHLLADSVCAMMFIRAWADLTLTRNMMAPPLFHSLPPRRFANHRLIANNNQLLSHYIKSCALTATPSNVAKDRMVTVTLLFPDPLVRARNNEPGVSAFEILAGVFWVCVSLAKGKRNEFMDMSLCVDVRKLLRLDQSYFGNCMVYHKVHYSKPVKTKDRLLSLSHAVQEIHKTTKRLDYDTVMDLIEWLSSNNSNPISNGSDLVCANLENMASSRPMMFEEDLILSHVSCYVEGPVAGGGQFIVLPSPSCEGPMSRVVMVSLPSREMVKVLDDDLLLSFAPVLLMDYTKQTY
>PAC:20203097
MPSCSVRETSKCIVYPEKKSTVSDLRLSVSDLPMLSCHYIQKGVLLTRPPPSFSFDDLVSSLRRSLSSTLSLFPALAGRFTTTPSGHIYITCNDAGVEFVSASAKHVAVSDVLSPGKDVPLLVREFFVFERLVSYNGHHKPLAAVQVTELDDGVFVGCTVNHSVTDGTSFWHFFNTFADVTSGACKIKHLPDFSRHTVFDSPAVLPVPPGGPRVTFDADQPLRERIFHFSREAISRLKQRTNYRVNGIETAVKTEEILGKVSNDGRKFNGNGEEANGKITKGFLKSDTNKKNYDRTAEISSFQSLSAQLWRSVTRARNLDPSKTTTFRMAVNCRHRLEPKMDPYYFGNAIQSIPTMASAGDLLTKDLRWSADQLHRNVVAHDDATVRRGIAAWESDPRVFPLGNPDGASITMGSSPRFPMYDNDFGWGKPLAVRSGGANKFDGKISAFPGREGNGSVDLEVVLAPETMAGIENDPEFMQYVSQVSYDC
>PAC:20203415
MELQIPPPVTSDSLMFKVHRREPELVTPAKATPRELKLLSDIDDQEGLRFHIPSIFFYRNNPTAYSDPVAVIRRALAETLVYYYPLAGRLREGPNRKLAVDCSGEGVLFIEADADVTLVEFEKKDVLRPPFPCFEELLFDIEGSSEVLNAPMILIQVTRLKCGGFIFAVRINHAISDAAGLSLFLKTIGEFARGSHVPTVAPVWERHLLSARVPASVTHVHREYDEQDTESATVDGGALVSRSFFFGGAEMSVIRRLLPQNLANRSTDLETLTAFLWRYRTAALRTDPNKEMRVIFIVNARSKFRNPPLPPGYYGNAFAFPVAIATVRELTEKPLEFALRLVKEAKWSVTEEYMRSLADLMVTKGRPRFSIDGAYLISDVRNFADIQFGVWGKPVYGGIGTAGVVDFPGSSFYVSFENSKGERGIVVPVCLPEKAMKRFVEELDGVFNGQLELNGRSKIPDKLIMSSL
>PAC:20186733
MDIKVEILSREVIKPSSPTPNDMRILNLSLLDILSPPLGNILEAEEISVYNITNLLPFVECNDEGAVFIEAQVDHLLSEFLKFSVLESLKPLVPVEAKSGEVVIWPVLLIQANFFSCGGLVITICISHKITDATSLAMFIRGWAESSRGLGITLIPSFTASDFFPTPPPDELPLKQIDHKVEVEEMMNYVTKRFVLDASKMKKLRAKASSNLVKNPTRVEAVTALLWRCATKASRVSSREPRTSVLQILVSLRGKVDTLSENTIGNMLSIMILKNEEAKIEGIQHVVDEFRRAKENFGLSCKEMSKPSSRILEVWEEIRKVYGMGNEVDLWMSNSWCKLGLYEADFGWGNPVWLTGRGTSNINNLMLLIDAKDGVGIEAWVTLTKEHMLLFECDDELLESASLNPHVLI
>PAC:20187734
MDLKVEEVNREVIKPASASATPHSDQIQLSLFDFSSPPAYVSAVLFYNNTNNLNSEIISQKLKTSLSNTLSRFYPLAGRIKGDSISCNNEGAVFIEASTDLTLSDFLKTLNTDSLEKIFPTTAPGESPRAWPLLSVKVSFFGSGSGVAIAVCVSHLICDAASLFTFVSDWAATTKGKSSDVKSPQFASTTIYPPPHISFQVPNMDALTHFSEKCVTKRFVFESSKIPELKRVATSETVAFPSRVEAITALIWRCATKASPRSDWSSMMNQAIDLRLRIPSDVLSQDAIGNLLTFFFLKKEPGSDMEIDKIVAEFRKAKEGVNEMIKESVTTSTLTQNLMKVTVDFISELKTKTNLYGVTSWCNRRFYEVDFGMGGPVWVGTRAAVPDSQLYVLLMDAKDGESVEALVGLPEQDMAVFERDQELLAYATLNPPILI
>PAC:20188306
MEIKVEILSREVIKPSYPTPNDMRILNLSLLDILSSPLYTGALLFYAADLQNLIRSSSEETSLKLKKSLSTTLPIFYPLAGRIMGSFIECNDEGAVFIEARVDHLLSEFLKCSVPDSLKPLVPFEAKSREAVIWPVLLIQANFFSCGGLVITICISHKITDATSLAMFIRGWAESSRGLGITLIPSFTASDFFPIPPPDEFPLKPIEHKVQVEEMMNCVTKRFVLDASKMKKLRAKASSNLVKNPTSVEAVTALLWRCATKASRVSSREPRTSVLQILVSLRGKVDTLSENTIGNMLSIMILKNEEAKIEGIQHVVDEFRRAKENFGLSCKEMSKPSSRILEVWEEIRKVYGMGNEVDLWMSNSWCKLDLYEADFGWGNPVWVTGRGTSNINNLMLLIDAKDGVGIEAWVTLTKEHMSLFECDDELLESASLNPPVLI
>PAC:20184816
IWALALMSMLTQHYYLPLAGHVKWNPQDPKPCIVVLPYDTVSLIVAESSDADFSSVSRKGLRPAKEICSLVPELPVSGDSPQLLSLQVTLFPNQGFSIGIVSHHVFMDAITAQMFIKSWAHICKHGAKALPEDLTPFLDRTVIYVPDGLEEKMLEFLLYLSEDKDNGRTLKLPLTEETDADVYRVTLELTLENVEKLKERAKNGSNRPHLELHLPTFVIVNAYLWTCLVKARGGETGYVPRTYSGVCVFAISCLGYKAKSFLGEDGFINVDRMKNIERGTQVWGVSGSKRFSVYESDFGWGRPVNSEIVSIDQCQLLSMSEMRDETGGVEIGLCLKKSEMDVFIYLFKKGFENYFFLMASYSRL
>PAC:20180124
MPSLDNSVTIISRSRVFPDQKSTLVDLKLSVSDLPMLSCHYIQKGCLFTRPHLPTHALLSHLKHSLSLTLSHFPPLAGRLSTSEDGHVFLSCNDAGADFVFADANSVHVRDVIGGIDVPDVVKEFFSYDRAVSYEGHHRPILAVQVTELIDGVFIGCSVNHAVTDGTSLWNFINTFAEVSRGVENVTRKPDFTRESVLISQAVLKVPQGGPKVTFNENEPLRERIFSFTREQILELKSMVNKKRNWTVINGVEVLGKQSNDKLNGKENGKLTEMLESLFGRNDAVSKTVSKTVDTVEISSFQSLCALLWRAITRARKLPSSKTTTFRMAVNCRHRLSPKLEPEYFGNAIQSVPTFATAGDVLSRDLRWIADQLNQNVAAHQDGKIRSVVADWEANPRCFPLGNFDGASVTMGSSPRFPMYDNDFGWGRPVAVRSGRSNKFDGKISAFPGRDGNGSVDLEVVLAPETMAGIESDGEFMRYVSKI
MSAKEETIMEEERVRFICKRTVVSTKQVEPGQLYRLSVLDHVMEPNHIRLVYYYRCSKTREPGEITKKLRESLAYTLNCYPIITGRLVKEIDGTDEKNELNRQWKVKSNDSGMRMVEARATGTVEEWLRNVNREEELKLVHWEDIYHDLPYYWSTFYVQVTEFESGGLAIGLSCSHLLADSVCAMMFIRAWADLTLTRNMMAPPLFHSLPPRRFANHRLIANNNQLLSHYIKSCALTATPSNVAKDRMVTVTLLFPDPLVRARNNEPGVSAFEILAGVFWVCVSLAKGKRNEFMDMSLCVDVRKLLRLDQSYFGNCMVYHKVHYSKPVKTKDRLLSLSHAVQEIHKTTKRLDYDTVMDLIEWLSSNNSNPISNGSDLVCANLENMASSRPMMFEEDLILSHVSCYVEGPVAGGGQFIVLPSPSCEGPMSRVVMVSLPSREMVKVLDDDLLLSFAPVLLMDYTKQTY
>PAC:20203097
MPSCSVRETSKCIVYPEKKSTVSDLRLSVSDLPMLSCHYIQKGVLLTRPPPSFSFDDLVSSLRRSLSSTLSLFPALAGRFTTTPSGHIYITCNDAGVEFVSASAKHVAVSDVLSPGKDVPLLVREFFVFERLVSYNGHHKPLAAVQVTELDDGVFVGCTVNHSVTDGTSFWHFFNTFADVTSGACKIKHLPDFSRHTVFDSPAVLPVPPGGPRVTFDADQPLRERIFHFSREAISRLKQRTNYRVNGIETAVKTEEILGKVSNDGRKFNGNGEEANGKITKGFLKSDTNKKNYDRTAEISSFQSLSAQLWRSVTRARNLDPSKTTTFRMAVNCRHRLEPKMDPYYFGNAIQSIPTMASAGDLLTKDLRWSADQLHRNVVAHDDATVRRGIAAWESDPRVFPLGNPDGASITMGSSPRFPMYDNDFGWGKPLAVRSGGANKFDGKISAFPGREGNGSVDLEVVLAPETMAGIENDPEFMQYVSQVSYDC
>PAC:20203415
MELQIPPPVTSDSLMFKVHRREPELVTPAKATPRELKLLSDIDDQEGLRFHIPSIFFYRNNPTAYSDPVAVIRRALAETLVYYYPLAGRLREGPNRKLAVDCSGEGVLFIEADADVTLVEFEKKDVLRPPFPCFEELLFDIEGSSEVLNAPMILIQVTRLKCGGFIFAVRINHAISDAAGLSLFLKTIGEFARGSHVPTVAPVWERHLLSARVPASVTHVHREYDEQDTESATVDGGALVSRSFFFGGAEMSVIRRLLPQNLANRSTDLETLTAFLWRYRTAALRTDPNKEMRVIFIVNARSKFRNPPLPPGYYGNAFAFPVAIATVRELTEKPLEFALRLVKEAKWSVTEEYMRSLADLMVTKGRPRFSIDGAYLISDVRNFADIQFGVWGKPVYGGIGTAGVVDFPGSSFYVSFENSKGERGIVVPVCLPEKAMKRFVEELDGVFNGQLELNGRSKIPDKLIMSSL
>PAC:20186733
MDIKVEILSREVIKPSSPTPNDMRILNLSLLDILSPPLGNILEAEEISVYNITNLLPFVECNDEGAVFIEAQVDHLLSEFLKFSVLESLKPLVPVEAKSGEVVIWPVLLIQANFFSCGGLVITICISHKITDATSLAMFIRGWAESSRGLGITLIPSFTASDFFPTPPPDELPLKQIDHKVEVEEMMNYVTKRFVLDASKMKKLRAKASSNLVKNPTRVEAVTALLWRCATKASRVSSREPRTSVLQILVSLRGKVDTLSENTIGNMLSIMILKNEEAKIEGIQHVVDEFRRAKENFGLSCKEMSKPSSRILEVWEEIRKVYGMGNEVDLWMSNSWCKLGLYEADFGWGNPVWLTGRGTSNINNLMLLIDAKDGVGIEAWVTLTKEHMLLFECDDELLESASLNPHVLI
>PAC:20187734
MDLKVEEVNREVIKPASASATPHSDQIQLSLFDFSSPPAYVSAVLFYNNTNNLNSEIISQKLKTSLSNTLSRFYPLAGRIKGDSISCNNEGAVFIEASTDLTLSDFLKTLNTDSLEKIFPTTAPGESPRAWPLLSVKVSFFGSGSGVAIAVCVSHLICDAASLFTFVSDWAATTKGKSSDVKSPQFASTTIYPPPHISFQVPNMDALTHFSEKCVTKRFVFESSKIPELKRVATSETVAFPSRVEAITALIWRCATKASPRSDWSSMMNQAIDLRLRIPSDVLSQDAIGNLLTFFFLKKEPGSDMEIDKIVAEFRKAKEGVNEMIKESVTTSTLTQNLMKVTVDFISELKTKTNLYGVTSWCNRRFYEVDFGMGGPVWVGTRAAVPDSQLYVLLMDAKDGESVEALVGLPEQDMAVFERDQELLAYATLNPPILI
>PAC:20188306
MEIKVEILSREVIKPSYPTPNDMRILNLSLLDILSSPLYTGALLFYAADLQNLIRSSSEETSLKLKKSLSTTLPIFYPLAGRIMGSFIECNDEGAVFIEARVDHLLSEFLKCSVPDSLKPLVPFEAKSREAVIWPVLLIQANFFSCGGLVITICISHKITDATSLAMFIRGWAESSRGLGITLIPSFTASDFFPIPPPDEFPLKPIEHKVQVEEMMNCVTKRFVLDASKMKKLRAKASSNLVKNPTSVEAVTALLWRCATKASRVSSREPRTSVLQILVSLRGKVDTLSENTIGNMLSIMILKNEEAKIEGIQHVVDEFRRAKENFGLSCKEMSKPSSRILEVWEEIRKVYGMGNEVDLWMSNSWCKLDLYEADFGWGNPVWVTGRGTSNINNLMLLIDAKDGVGIEAWVTLTKEHMSLFECDDELLESASLNPPVLI
>PAC:20184816
IWALALMSMLTQHYYLPLAGHVKWNPQDPKPCIVVLPYDTVSLIVAESSDADFSSVSRKGLRPAKEICSLVPELPVSGDSPQLLSLQVTLFPNQGFSIGIVSHHVFMDAITAQMFIKSWAHICKHGAKALPEDLTPFLDRTVIYVPDGLEEKMLEFLLYLSEDKDNGRTLKLPLTEETDADVYRVTLELTLENVEKLKERAKNGSNRPHLELHLPTFVIVNAYLWTCLVKARGGETGYVPRTYSGVCVFAISCLGYKAKSFLGEDGFINVDRMKNIERGTQVWGVSGSKRFSVYESDFGWGRPVNSEIVSIDQCQLLSMSEMRDETGGVEIGLCLKKSEMDVFIYLFKKGFENYFFLMASYSRL
>PAC:20180124
MPSLDNSVTIISRSRVFPDQKSTLVDLKLSVSDLPMLSCHYIQKGCLFTRPHLPTHALLSHLKHSLSLTLSHFPPLAGRLSTSEDGHVFLSCNDAGADFVFADANSVHVRDVIGGIDVPDVVKEFFSYDRAVSYEGHHRPILAVQVTELIDGVFIGCSVNHAVTDGTSLWNFINTFAEVSRGVENVTRKPDFTRESVLISQAVLKVPQGGPKVTFNENEPLRERIFSFTREQILELKSMVNKKRNWTVINGVEVLGKQSNDKLNGKENGKLTEMLESLFGRNDAVSKTVSKTVDTVEISSFQSLCALLWRAITRARKLPSSKTTTFRMAVNCRHRLSPKLEPEYFGNAIQSVPTFATAGDVLSRDLRWIADQLNQNVAAHQDGKIRSVVADWEANPRCFPLGNFDGASVTMGSSPRFPMYDNDFGWGRPVAVRSGRSNKFDGKISAFPGRDGNGSVDLEVVLAPETMAGIESDGEFMRYVSKI
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University