Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Thalictrum thalictroides
Species name
Rue-anemone (Thalictrum thalictroides) is a species of flowering plant in the family Ranunculaceae (buttercup family).
Species info
Gene sequence info
Thalictrum_thalictroides.COMT.fa
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>rna-gnl|WGS:JABWDY|TTHA_TU.03488.mRNA
MAVQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIIFQAGPEAQLSPAEIVTKIPTKNPNAAIALDRILRMLGASSILSVSTTKNGRVYGLTDESRCLVADKNGVSVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFAYAGKEQRVNKSFNQAMGAGSTIAFDEVFKVYKGFHDLKELVDVGGGIGTSLSNIIFKYPHIKGINFELPHVIADAPNYPGVEHIAGNMFEGVPNAQNILLKWVLHDWDDERSIKILQNCWKALPEGGTVIVVEFVLPQILGNNAESFNALTPDLLMMTLNPGGKERTTIEFVGLAKAAGFAETKFFPISQGLHVMEFHKATAGATS
>rna-gnl|WGS:JABWDY|TTHA_TU.13900.mRNA
MGSNQNQLNLTPQQEEEACSHAMQLASASVLPMVLKAAIELDVLEIIAKAGQGAYLSPTEIASQLVTTNNIIHAPIILDRILQLLASYKVLTCNLHTLKDGGEVDRLYGLAPVCKFLVKNEDGVSMAPLVLMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMSAFEYHGTDSRFNKVFNRGMSEHSTITMKKILDMYKGFDNLETLVDVGGGVGATINMIITKHPTIKGTNFDLPHVIQDAPEYPGVKHIGGDMFVSVPKGDAIFMKWILHDWSDEHCVKILKKCYEAMPQNGKMIIVESILPVFPETNLAANSVFQLDNIMLAHNPGGKERSEKDFEALSIGAGFATFKVICGAFNSWVMEFCK
>rna-gnl|WGS:JABWDY|TTHA_TU.18103.mRNA
MDSWFHLKDTVLEGGNAFSRAHGMHAFEYPGGINFDLPHVIAHAPSYPGVEHIGGNMFESVPTGEVIFMKWILHDWSDEKCLKVLKNIYKALPEQGKLIVVEVILPVAAETSTIAKGFFEIDVCMMTLNPGGKERTEEEFHTLAKQAGFARMEKLCCVYNYWIIEFYKL
>rna-gnl|WGS:JABWDY|TTHA_TU.22900.mRNA
MADETSETRNDARLAILELANMISVPMSLNAIVRLKVPDTIWQSGSNIPLSASDILARVQPTGDSENLQRILRMLTSYDVFTEHVINGSERKYSLTEVGKTLVTDQEGLSYASYVLQHHQDALIRSWPLVHEAVVDPTVEPFVKANGESAYGYYGENHEMNELMQKAMSGVSVPFMKAILDAYDGFDGVSRLVDIGGSAGDCLRMILQKHPHVKEGINFDLPEVVAKAPLISGVKHVGGDMFKGIPQADAIFMKWILTTWTDDECKAIMKNCYEALPKGGKLIACEPVLPHESDSSHRTRALLEGDIFVMTIYRAKGKHRTEEEFRQLGLSTGFTQYRAIYIDHFYTVLEFIK
>rna-gnl|WGS:JABWDY|TTHA_TU.24255.mRNA
MASTGNQCKTSNEDENFQYAMQLSSASVLPVAMQVAVELELFEIIAKRGPNAKLSPKEIASNLSTQNPNAHIMLDRIFRLLTSYSVMTSSLVTNKDGEAERLYGLAPVCKYFVNNEDGVSMCPLLLFAQDKILMESRCYLKDAILEGGIPFNKAHGMHAFEYGFEGLKEVVDVGGGVGITSSLITSKYPAIRCINFDLPHVIEQSQTYPGVVHIGGNMFDSVPNGETIFMKWILHDWSDENYSLQAIYMLQGKLEPSELGGRDQASPESF
>rna-gnl|WGS:JABWDY|TTHA_TU.26798.mRNA
MDSIANHQKLANEEENYLLVMELVQAPILPMVIRVAVELGVFDTIAKHGSGSQMSSKSIVFHLPTQNPNAANLLDRILRLLCSYNILTSSMVTRNDGHVERLYGLAPLCKFFLKDEDGFSLYTDILMFQEKAVMDGWGHLKDAILEGGTPFNNAHGMNVFEYAERDARFYQVMTAGMFNHTAIIMKKILETYTGFENLREIVDVGGGVGTSIGLITSKYPTIKGINFDLPHVVAHAPSYPGVKHVGGNMFESIPSCETIFMKWILHDWSDEVVVDLLKNCYKALPDQGKVIVMDVILPAAIETNSTAKCAFLSDLLMMLGSEDGKERTEEEFFALAKEAGFVSAKKVCCVYNCWVMEFYKAQKN
>rna-gnl|WGS:JABWDY|TTHA_TU.29630.mRNA
MVLKAAIELDVLEIIAKAGHGAYIAPTEIASQLCTSNSQASIILDRILRLLASYKILTCNLRTLEDGSVERLYGLAPVCKFLVKNEDGVSMAPLVLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTVTMKKLLELYKGFEGLKSVVDVGGGTGATVNMIVTKHPTIKGINFDLPHVIDDAPAYPGVEHIGGDMFVSVPKGDAIFMKWILHDWSDEHCVNVLKNCYEAVPADGKVIIVESILPVFPETNLASNACFQLDNIMLAHNPGGKERTEKDFETLSVKAGFTGFKVVCSAFGSWVMEFCK
MAVQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIIFQAGPEAQLSPAEIVTKIPTKNPNAAIALDRILRMLGASSILSVSTTKNGRVYGLTDESRCLVADKNGVSVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFAYAGKEQRVNKSFNQAMGAGSTIAFDEVFKVYKGFHDLKELVDVGGGIGTSLSNIIFKYPHIKGINFELPHVIADAPNYPGVEHIAGNMFEGVPNAQNILLKWVLHDWDDERSIKILQNCWKALPEGGTVIVVEFVLPQILGNNAESFNALTPDLLMMTLNPGGKERTTIEFVGLAKAAGFAETKFFPISQGLHVMEFHKATAGATS
>rna-gnl|WGS:JABWDY|TTHA_TU.13900.mRNA
MGSNQNQLNLTPQQEEEACSHAMQLASASVLPMVLKAAIELDVLEIIAKAGQGAYLSPTEIASQLVTTNNIIHAPIILDRILQLLASYKVLTCNLHTLKDGGEVDRLYGLAPVCKFLVKNEDGVSMAPLVLMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMSAFEYHGTDSRFNKVFNRGMSEHSTITMKKILDMYKGFDNLETLVDVGGGVGATINMIITKHPTIKGTNFDLPHVIQDAPEYPGVKHIGGDMFVSVPKGDAIFMKWILHDWSDEHCVKILKKCYEAMPQNGKMIIVESILPVFPETNLAANSVFQLDNIMLAHNPGGKERSEKDFEALSIGAGFATFKVICGAFNSWVMEFCK
>rna-gnl|WGS:JABWDY|TTHA_TU.18103.mRNA
MDSWFHLKDTVLEGGNAFSRAHGMHAFEYPGGINFDLPHVIAHAPSYPGVEHIGGNMFESVPTGEVIFMKWILHDWSDEKCLKVLKNIYKALPEQGKLIVVEVILPVAAETSTIAKGFFEIDVCMMTLNPGGKERTEEEFHTLAKQAGFARMEKLCCVYNYWIIEFYKL
>rna-gnl|WGS:JABWDY|TTHA_TU.22900.mRNA
MADETSETRNDARLAILELANMISVPMSLNAIVRLKVPDTIWQSGSNIPLSASDILARVQPTGDSENLQRILRMLTSYDVFTEHVINGSERKYSLTEVGKTLVTDQEGLSYASYVLQHHQDALIRSWPLVHEAVVDPTVEPFVKANGESAYGYYGENHEMNELMQKAMSGVSVPFMKAILDAYDGFDGVSRLVDIGGSAGDCLRMILQKHPHVKEGINFDLPEVVAKAPLISGVKHVGGDMFKGIPQADAIFMKWILTTWTDDECKAIMKNCYEALPKGGKLIACEPVLPHESDSSHRTRALLEGDIFVMTIYRAKGKHRTEEEFRQLGLSTGFTQYRAIYIDHFYTVLEFIK
>rna-gnl|WGS:JABWDY|TTHA_TU.24255.mRNA
MASTGNQCKTSNEDENFQYAMQLSSASVLPVAMQVAVELELFEIIAKRGPNAKLSPKEIASNLSTQNPNAHIMLDRIFRLLTSYSVMTSSLVTNKDGEAERLYGLAPVCKYFVNNEDGVSMCPLLLFAQDKILMESRCYLKDAILEGGIPFNKAHGMHAFEYGFEGLKEVVDVGGGVGITSSLITSKYPAIRCINFDLPHVIEQSQTYPGVVHIGGNMFDSVPNGETIFMKWILHDWSDENYSLQAIYMLQGKLEPSELGGRDQASPESF
>rna-gnl|WGS:JABWDY|TTHA_TU.26798.mRNA
MDSIANHQKLANEEENYLLVMELVQAPILPMVIRVAVELGVFDTIAKHGSGSQMSSKSIVFHLPTQNPNAANLLDRILRLLCSYNILTSSMVTRNDGHVERLYGLAPLCKFFLKDEDGFSLYTDILMFQEKAVMDGWGHLKDAILEGGTPFNNAHGMNVFEYAERDARFYQVMTAGMFNHTAIIMKKILETYTGFENLREIVDVGGGVGTSIGLITSKYPTIKGINFDLPHVVAHAPSYPGVKHVGGNMFESIPSCETIFMKWILHDWSDEVVVDLLKNCYKALPDQGKVIVMDVILPAAIETNSTAKCAFLSDLLMMLGSEDGKERTEEEFFALAKEAGFVSAKKVCCVYNCWVMEFYKAQKN
>rna-gnl|WGS:JABWDY|TTHA_TU.29630.mRNA
MVLKAAIELDVLEIIAKAGHGAYIAPTEIASQLCTSNSQASIILDRILRLLASYKILTCNLRTLEDGSVERLYGLAPVCKFLVKNEDGVSMAPLVLMNQDKVLMESWYHLKDAVLDGGIPFNKAYGMTAFEYHGTDPRFNKVFNRGMSDHSTVTMKKLLELYKGFEGLKSVVDVGGGTGATVNMIVTKHPTIKGINFDLPHVIDDAPAYPGVEHIGGDMFVSVPKGDAIFMKWILHDWSDEHCVNVLKNCYEAVPADGKVIIVESILPVFPETNLASNACFQLDNIMLAHNPGGKERTEKDFETLSVKAGFTGFKVVCSAFGSWVMEFCK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University