Enzyme

One of three specific glucosyltransferases in pea (Pisum sativum) that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91, flavonol 3-O-glucosyltransferase and EC 2.4.1.240, flavonol-3-O-glycoside glucosyltransferase). TDP-glucose can replace UDP-glucose as the glucose donor but the reaction proceeds more slowly.

UGT79B30

Enzyme

UDP-glycosyltransferase 79B30

Enzyme name

EC:2.4.1.239

Entry

Transferases;Glycosyltransferases;Hexosyltransferases

Class

UDP-glucose:flavonol-3-O-beta-D-glucoside 2''-O-beta-D-glucosyltransferase

Sysname

UDP-glucose + a flavonol 3-O-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [RN:R07339]

Reaction

UDP [CPD:C00015];flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside [CPD:C15581]

Product

Species information

Thalictrum thalictroides

Species name

Rue-anemone (Thalictrum thalictroides) is a species of flowering plant in the family Ranunculaceae (buttercup family).

Species info

Gene sequence info

Thalictrum_thalictroides.UGT79B30.fa

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>rna-gnl|WGS:JABWDY|TTHA_TU.04315.mRNA
MAAWFTRENVCEAVKLVMDEGSEVGEQVRANHHKWKELLLSEGLESSYIDNLISKLQDMVK
>rna-gnl|WGS:JABWDY|TTHA_TU.04316.mRNA
MREEFTRENVYETVKLVMDEGSEVGKQVRENHHKWKKFLLSEGIESSYIDCKICAFDQGSILRLFSDY
>rna-gnl|WGS:JABWDY|TTHA_TU.08880.mRNA
MEGPNCEYMKNLYNKQVLLTGPILPESSTTNLKSPISSPWRLGSTTTNLKSPITRMFCEPWWFVVYMAGIIEKTMKTTKLMWDASRRNEQRQKQIDLIYIYN
>rna-gnl|WGS:JABWDY|TTHA_TU.10618.mRNA
MATTATFHIVMFPWFAMGHITPFLQLANKLAERGHKVSFFIPKNTQNKVNHLNLHPHLITFIPLIVPQVDGLPVGTETMSDLPVTKSPLLLQALDLTQEQVEPLVQKLKPDFIFFDFCYWITTLARRLGIISIMFAVISVVAFLSICNLKLYNYKPFEAKDQSFLSTEIDGRISFFNRVTTSLRESEALCFRSCSEMEGPNCEYLKNLYNKQVLLTGPILPESSNNPLEERWSKWLGGFEKGSVVYCAFGSECVLEKNQFQELVLGLERTGLPFLVRLKPPVGAETLEEALPEGFQERVKGRGVVHGGWVQQQQILNHPSVGCFVSHGGSSSIWESLVSSCQIIFVPQSGAQFADTKLMSEDLKVAVNVQRREEDGWFTRESLCEAVKLVMDEGSEVGKQVRENHHKWKEFLLSEGLDSSYIDNLIIKLQDILLSKA
>rna-gnl|WGS:JABWDY|TTHA_TU.10856.mRNA
MSTTETVHVVMFPWFAMGHIIPYLQLANKLAERGHKVSFFIPKNTQNKLDHLNLHPHLITFIPLIVPHVDGLPVGTETMSDLPITKSPLLLQALDLTQEQVEPLVQKLKPHFIFFDFCYWITTLACKLGIISVNFNVVSPVARASLFNPRLFDYKPFEAKGRSFLSTEIDGRISFINRLMTSGRESEALCFRACSEMEGPYCEYLKNLYNKQVLLTGPILPEPSHNPLEERWTKWLGGFEKGSVVYCAFGSECVLEKNQFQELVLGLEQTGLPFLVCLKPPVGAETLEEALPEGFQEKVKGIGVVHGGWVQQQQILNHPSVGCFVSHGGSASIWESLVSYCQIILVPHSGAQFADTKFMTEELKVAVNVERREEDGWFTRESVCEAVKLVMDEGSEVGEQVRANHHKWKEFLLSEGLEPSYINNLIIKLQDMLK
>rna-gnl|WGS:JABWDY|TTHA_TU.13317.mRNA
MSDLPVTKSPLLLQAFDLTQEQVESTLLKLKPDFIFFDFGYWIPSLAHTLGIKSVYFSVVSAVVRACHMRDLQLSNLKPFHAKDRPMLSTEIDGRISFLNRLLTASKESDALCFRACSEMEGAECDYLKNVYNKEILLTGPILPEPSHTPLEERWAKWLDGFKQESVVYCAFGSECVLEINQFQELVLGLEQTGLPFLVRLKPPVGAETIEEALPEGFEERVRGRGVVHEGWVQQQQILNHPSVGCFVSHGGSASIWESLASTCQIVFVPHFGAQFIDSEFITENLKASVTVERRDEDGWFTRESVCKAVKLVMDEGSEVGEEIRASHHKWKEFLLSAGIESSYINNLILKMQDMLK
>rna-gnl|WGS:JABWDY|TTHA_TU.18464.mRNA
MVTSTVVLHVAMLPWFATGHITPFVTLSNKLAEKGHKISLFIPAKTQNKFNHLNLHPHLIQFIPVTVPHVDGLPSGAETMSDISLDKFNFLQHAFDLTQDEVEVAFRSLKPNFVIYDFAYWIPKIAHPLGIKAIFFSTCGAIPLLSFVPSMMTPADDHQRLELLPVPEIYPCSSSAKLLPYETKNSGIDALTEYNGRISIVMRVFTAMTESDILCFRCCKEMENPYLNYLQSHYNDKPVLAIGPLLSSDTSTILEERWANWLGCFEKGSVVYCAFGSECVLEKNQFQELVLGLEKTGFPFLVRLRPPFGAETLEEALPEGFQERVRGRGVVHERWVQQKLILSHPSVGCFVSHCGFGSVWDSLMSTCQIVLVPQSSDQFIQTKFLTKGLKVAVNVERREEDGWYTKEDVCKAVKLVMDEGSEVGEEVRANHIKLRQEFLVEGFESSYIDNFINKLQDHCHA
>rna-gnl|WGS:JABWDY|TTHA_TU.19793.mRNA
MEGPYCEYLKNLYNKEILLTGPILPEASHTPLEEKWAKWLDGFKQGSVVYCAFGSECVLEKNQFQELVLGLEQTGLPFLVRLKPPVGTENLEEALPEGFEERVRGRGVVHGGWVQQQQILNHSSVGCFVSHGGSASIWESLVSPCQIIFVPQSGAQFPHTKFMTKDLKVALSVERREEDGWFTRESVCKAVKLVMDEGSEVSKQVRANHHKWKEFLLSEGLESSYIDNLIIKLQDMLR
>rna-gnl|WGS:JABWDY|TTHA_TU.21686.mRNA
MAQSKLHIAMNPWFAFGHLNAFLHLSNLIAARGHRISFLTPKKAQAKLEDFNNHPDLIKFIPLTVPHVEGLPVGAETTNDVPDHQGSLIMTAMDKTRPEVEAILKDLKPDVVFFDFTYWLPEVTKQLGIKSLQYCITSASTVAYILVRSSTKLDEGQQLTEDELMKPPADFPPSSTITLHRHEARALSFTTHRTYGEGISFHERIVYALRDADGYGFRSCREIEGTYCDYIENHFKKPSILFGPTIPQLPTTPLDENKAKWLGQFKPGSVVFCAFGSECVMEKNNFQELLLGIELTGLPFFIGLKPPSGTETIDEALPEGFKERVQGRGIVEGGWIQQLQIMAHPSVGCFISHGGLSSVTEALVNNTQLVIIPQRGDQYIIARLMSGDMKVAVEVEMREEDGWYSKENVCTTVKSVMDEGNETGKQIRANKLKLKEFLLDENLESTYLDSALDRLKETLLK
>rna-gnl|WGS:JABWDY|TTHA_TU.24346.mRNA
MSITETIHVVMFPWFAMGHITPYLQIANKLGERGHKVSFFIPKNTQNKVEHLNLHPHLITFIPLIVPQVDGLPVGTETMSDLPVTKSPLLLHALDLTQEQVEPFVRKLKPDFIFFDFCYWITTLARRLGIISIYFSVVSPVARASRCNPQLFDYKPFEAKERSLFTTEIEGRISFINRLATSGRESEALCFRACSEMEGPYCEYLKNVYNKQILLTGPILPEPSKTLLEERWSKWLGDFEKGSVVYCAFGSECVLEKNQFQELVLGLELTGLPFLVRLKPPVGVESLEEALPEGFEERVKGRGIVHGGWVQQQQILNHPSVGCFVSHGGSSSIWESLVSSCQIILVPHSGAQFTDTKFMSEELKVAVNVERREEDGWFTRESVCEAVKLVMDERSEIGKQVRANHYKWKEFLLSEGLESSYIDNLIIKLQDMLK
>rna-gnl|WGS:JABWDY|TTHA_TU.27484.mRNA
MAATATFHVVMFPWFAMGHITPYLQLANKFAERGHKVSFFIPNNTQNKVNHLNLHPHLITFIPLIVPQVDGLPVGTETMSDLPVTKSPLLLRALDLTEEQVEPLVQKLKPDFIFFDFCYWITTLARRLGIISIYFSVVSPVAEASLFNPRFFDFKPFEAKGRSFLSTEIDGRISFINRLATSGRESEALCYRGCSEMEGPYCEYLKNVYNKQVLVTGPILPEPSHNPLEERWSKWLGGFEKGSVVYCAFGSECVLEKNQFQELVLGLEQTGLPFLVRLKPPVGAETLEEALPEGFQEKVKGRGIVHGGWVQQQQILNHPSVGCFVSHGGSSSIWESLVSSCQIILVPHSGAQFADTKFMSEELKVAVNVERREEDGWFTRESVCEAVKLVMDESSEIGKQVRANHHKWKEFLLSEGLESSYIDNLIIKLQDMLK
>rna-gnl|WGS:JABWDY|TTHA_TU.31244.mRNA
MFPWFAMGHITPYLLLSNKLAERGNKISFFIPAKTQAKFNHFNIHPHLINFIPLTISQINGLPLDAETVADVPDMKNKTYLIAQALDLTQEQVQNALEKLKPDFIFYDFPYWVPTIAKPLGIKSIYFCPSGALPNVSLVPSMMKILEKGRDQCTDQELHQLTKIPEIYPGSSTVKPPSYERKGASFALTSFDDSISMVKRLFIAMKESDALCFRACYETESTYAEFLENQYDKQVLLTGPLLPKLSSNIPLLEERWSKWLDSFEKRSVVYCAFGSECVLEKEPFQELVLGLEKTGLPFLVRLRPPIGIDTVEEALPEGFEERVRGRGVVHGGWIQQQLILNHPSVGCFVGHCGSGSMWESLVSTCQLVLVPHFADQFINTKFMTKRLKVAVDVDRREEDGWFTREDVCKAVKLVMDEGSVVGEEIRANHHKLSQLFLGEGFETSYINNFILKLHDLLV
>rna-gnl|WGS:JABWDY|TTHA_TU.31250.mRNA
MFPWFAMGHITPFLHLSNKLAEKGHKVTFLIPSNTQNKFNHLNHHPHLIQFIPLAVPQVDGLPLGAETMSDIPVHQAPVLAVAFDLTKDQVKIALENLKPNLIFFDFAYWLPPLAESLGVKCIFFSVCSAVSTSYILVPARKLYKGQHFSEHDLIEPPKGYPSGSSMKHLLFEAKDGVYFSSEFNGGISFFHRLTTSMTESQALCYRACTEMEGPFIDYFEKQYNKEILLTGPILPKPSHTPLEERWAKWLDGFKQESVVYCAFGSECVMEKDQFQELVLGLEQTGLPFLVRLKPPFGCETLEEALPEGFEERVKGRGVVHGDWIQQQQILSHASVGCFVSHCGFGSMWESLVSSCQIVLVPQAGDQHANAKFMTKELKVAMDVERREEDGWFTKESVCKAVKLVMDEGSEVGEQVRANHLKWKDFLLTEDLETSYINNFMIKLHDILK
>rna-gnl|WGS:JABWDY|TTHA_TU.33475.mRNA
MAKFTEIGKTLEVGVQVLSMVMTSKFHVAMFPWFAVGHITPYLKLSNKLAERGFRVSFFIPTNTQSKFNHLNLHPHLIQFILLTVPHVDGLPLGAESSADVPVYKTHLLEQALDLTQDQVQIALEKFKPNVIFFDFAYWVPLVARPLGIKSVFASLTSAVTNVGLVPSLLPIVEKGIEHSTDQEREEFAKIPEIYPGSSSIKKPPYEIKGTSFAVIKYDGKISLVNRLLIAMKDSDALCFRACHETEGPYADFLENQYNKPVMLTGPLLTKTSTSTILEDRWANWLGGFEKGSVVYCAFGSECVLEKDQFQELVLGLEQTGLPFLVRLRPPVGADTLEEALPEGFEERVRNRGVVHGGWIQQELILSHPSVGCFIGHCGSGSMWESLISNCQLVLVPHFADQFLNTNFMTKQLKVAVNVERKEEDGWFARDGVCKAVKLVMDKGSEVGEEVMSNHLKLRNILLSEGFETSYINNCILKLHDLLTT
>rna-gnl|WGS:JABWDY|TTHA_TU.33687.mRNA
MFPWFAMGHITPYLKLSNKLAERGFRISFFIPAKTQIKFNHLNLHPHLIQFIPLTVPHVDGLPPGADSSADVRADKTHLIQQALDLTQDQVQIALEKFKPDIIFFDFAYWVPSVARPLGIKSIFFSVSGAMTNVGLVPSMLPIVEKGIEHSTYQELQELAKIIPETYQGSSSIKQPPYEIKGMSFALIKYEGRISLVHRLLIAMRDSDALCFRACHETEGLFADFLENQYNMPVLLTGPILTKTSPTMLEERWAKWLGGLEKGSVVYCAFGSECVLEKDQFQELVLGLEKTGLPFLARLRPPVGVVTLEEALPEGFEERVKGRGVVHGGWIQQELILSHPSVGCFVGHCGSGSMWESLVSTCQLVLVPHFADQFLNTNFMTKGLKVAVDVERREEDGWFTREGVCKAVKLVMDEESEVGEEVRSNHLKLSNILSSEGLETSYINNYILKLNDLLTS

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University