Enzyme
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
DFR
Enzyme
Dihydroflavonol 4-reductase
Enzyme name
EC:1.1.1.219
Entry
Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Sysname
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Thalictrum thalictroides
Species name
Rue-anemone (Thalictrum thalictroides) is a species of flowering plant in the family Ranunculaceae (buttercup family).
Species info
Gene sequence info
Thalictrum_thalictroides.DFR.fa
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>rna-gnl|WGS:JABWDY|TTHA_TU.06578.mRNA
MAEALLLVYEKPEAEGRYICSSHTITVQDFVEKLKSMYPNYYHPKQIAEGDEDWDLTSEKLLKLGWSYRPLEETIVDSIKDYQEKGIMQ
>rna-gnl|WGS:JABWDY|TTHA_TU.07959.mRNA
MGVEGETVCVTGAAGFIGSWLVMRLLERGYIVKATVRDPDNLKKNEVIKPTIEGMLNIMRSCLKAKTFRRLVFTSSAGTVNGEEHQQPEYDENCWTDIDFCRTKKMTGWMYFVSKTLAEKAAWEFAEQNNIDFISIIPTLVVGPFLTSSMPPSLITALSPITRNEAHYSILKQIQLVHIDDLCNAHIFLFEHPEAKGRYICSTYNATIMDVANLLRNKFPEYNVPTKFKDVDESLKTVAFSSKKLTDLGFSYKHTEGEDMFVEAVASCREKGLLPLCEETKVNDESYVEMKKQEVDITNKKQAELMAMKSNMVLTEAHSVKF
>rna-gnl|WGS:JABWDY|TTHA_TU.12346.mRNA
MEDSGKLVESEGNKGALLSFCVTGSTGYIGSWLVKSLLQRGYIVHATARDPGKVTCLLPLWNGGDRLKVFKADLKEEGSFDEAVKGCHGVFHVAASMEFSVIAKDNIENYVQSYILDPAIKGTLNLLKACVRAKSIKRVVFTSSISTITAKGINGRWKTIVDESCQTPMDHVWNMKTSGWVYVLSKLLSEKAAFKFADENGIDLISIIPTTVGGLFLTPTVPASVRVLLSPITGDSEFNAILQAVHNRMGSISVVHIEDICNAHIFLMEHTTAKGQYICSAHSCVMSQLVDSLALMYPCSGIQRSSEEQQDSIPSEVSSEKLKSLGFAYKYGPKEILQQSISSCLQHGYLKNPSNNDSYP
MAEALLLVYEKPEAEGRYICSSHTITVQDFVEKLKSMYPNYYHPKQIAEGDEDWDLTSEKLLKLGWSYRPLEETIVDSIKDYQEKGIMQ
>rna-gnl|WGS:JABWDY|TTHA_TU.07959.mRNA
MGVEGETVCVTGAAGFIGSWLVMRLLERGYIVKATVRDPDNLKKNEVIKPTIEGMLNIMRSCLKAKTFRRLVFTSSAGTVNGEEHQQPEYDENCWTDIDFCRTKKMTGWMYFVSKTLAEKAAWEFAEQNNIDFISIIPTLVVGPFLTSSMPPSLITALSPITRNEAHYSILKQIQLVHIDDLCNAHIFLFEHPEAKGRYICSTYNATIMDVANLLRNKFPEYNVPTKFKDVDESLKTVAFSSKKLTDLGFSYKHTEGEDMFVEAVASCREKGLLPLCEETKVNDESYVEMKKQEVDITNKKQAELMAMKSNMVLTEAHSVKF
>rna-gnl|WGS:JABWDY|TTHA_TU.12346.mRNA
MEDSGKLVESEGNKGALLSFCVTGSTGYIGSWLVKSLLQRGYIVHATARDPGKVTCLLPLWNGGDRLKVFKADLKEEGSFDEAVKGCHGVFHVAASMEFSVIAKDNIENYVQSYILDPAIKGTLNLLKACVRAKSIKRVVFTSSISTITAKGINGRWKTIVDESCQTPMDHVWNMKTSGWVYVLSKLLSEKAAFKFADENGIDLISIIPTTVGGLFLTPTVPASVRVLLSPITGDSEFNAILQAVHNRMGSISVVHIEDICNAHIFLMEHTTAKGQYICSAHSCVMSQLVDSLALMYPCSGIQRSSEEQQDSIPSEVSSEKLKSLGFAYKYGPKEILQQSISSCLQHGYLKNPSNNDSYP
Sheng jun & Dong yang Teams, Yunnan agriculture University