Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Tetracentron sinense
Species name
Tetracentron sinense is a species of flowering plant in the family Trochodendraceae.
Species info
Gene sequence info
Tetracentron_sinense.FLS.fa
Download
>evm.model.ctg000276_pilon_pilon.80
MEIERVQAIALLSMSNDTIPLEFIRSEKEQPAITTFHGPVPEIPTIDLSDSDEEKIVPLIDSTCREWGIFQAVNHGIPSDVISKLQSVGREFFELPQEEKEAYAKPPGSETVEGYGTKLQKEVEGKRAWVDHLFHNVWPPSRINYQFWPKNPPSYRFV
>evm.model.ctg000276_pilon_pilon.79
MEIERVQAIALLSMSNDTIPLEFIRSEKEQPAITTFHGPVPEIPTIDLSDPDEEKIVRLIDSACREWGIFQAVNHGIPSDVMSKLQSVGREFFELPQEEKEAYAKPPGSETVEGMELSFRRKEANEEYTKCMRGVVDKLFRSLSLGLGLEGHVVKEAVGGEELEYLMKINYYPPCPRPDLALGVVAHTDMCTFTVLVPNEVPGLQVFKDDRWFDAKYIPNALIVHIGDQIEILSNGRYKSVLHRTTVNKEKTRMSWPVFCQPPPEVTVGPLSQLVNEENPTKYKTKKYKDYMYCKLNKLPQ
>evm.model.ctg000515_pilon_pilon.285
MSMDTIPSEFIRSEKEQPAITTPAIDLSDPDQEKLVHSIAEASMEWGIFQVVNHGIPIDVVSELQSVGREFFELSEEEKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYREANEEYAKCMGHVVDKIFTSLSLGLGLEGNVLREAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEILSNGKYKGVLHRTTVNKEKTRMSWPVFFELPSELVVGPLPQLVNEDNPAKYKTKKYKEYVHCKLNKHPQ
>evm.model.ctg000515_pilon_pilon.287
MRSFTVATAFAWWPSSADLAAFFAKASSIGSLVLRLQTKAAYMFSMATLACDKYHVPPLFLKDGSRLNPRRLDAFLGLRRRRWWSRDPSEGNLLQDVGQEFIKLPQEEKKYANDPSSGKFKGYGTKMTRNHDEKVEWVDYYFHLMNHAIWPQKPASYREVTEEYAEELLKVTDVLLELLSEGLGLERKALKSSLRGEEIKLDMKINMYPPCPQPQLALGVEPHTDMSALTILIPNDVPGLQVWKDDNWVVVDYKPNVLFIHVVDQIEVGIEISPAIGEELEEEMADDNPIP
>evm.model.ctg000515_pilon_pilon.288
MEVERVQTLSSGGLDELPVQFVRPAHERPENSKAVEGISVPVISLSQPAEILVKEIYNACSQWGFFLVTEHGISPSLICRLQDVGQEFFKLPQGEKETYANDPSSGKFEGYGTKMTRNPDEKVEWIDYYFHLMSPTSKVNHAIWPQKPASYREVTEEYAEELLKVTDVLLELLSEGLGLERKALKSNLRGEEIELEMKINMYPPCPQPQLALGVEPHTDMSALTILIPNDVPGLQVWKDDNWIAVDYIPNALFIHIGDQIEVLSNGKYKSVLHRSLVNKDRMRMSWAVFCTPPLDASIGPLPQLVNDENPSKYSTKTYEEYRYRKFNKIPQEKVWVKKSDQSGTESDIKFAHEITADQHQGKDIDGSGRPQGNEVLTRRINLIEENDKEGYPNEGKE
>evm.model.ctg000515_pilon_pilon.290
MGVERVQAIASLSMSMDTIPSEFIRSEKEQPARTTYNGPVPEIPAIDLTSYSGSDESSSNSQKKKKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYREANEEYAKCMGHVVDKIFTSLSLGLGLEGNVLREAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFKDNSWFNANYIPNALIVHIGDQIEEKTRMSWPVFFEPPSELVVGPLPQLVNEENPAKYKTKKYKEYVHCKLNKLPQ
>evm.model.ctg000515_pilon_pilon.291
MGVERVQAIASLSMSMDTIPSEFIRSEKEQPARTTYNGPVPEIPAIDLSNPDREKLVHSIAEASMEWGIFQVVNHGIPIDVVSELQRVGREFFELSEEEKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYRFVLGQEANEEYAKCMGHVVDKIFTSLSLEAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFKDNSWFNANYIPNALIVHIGDQIEILSNGKYKGVLHRTTVNKEKTRMSWPVFFEPPSELVVGPLPQLVNEDNPAKYKTKKYKEYVHCKLNKLPQ
>evm.model.ctg000515_pilon_pilon.292
MGVERVQAIASLSMSMDTIPSEFIRSEKEQPARTTYNGPVPEIPAIDLSDPDQEKLVHSIAEASMEWGIFQVVNHGIPIDVVSELQRVGREFFELSEEEKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYREANEEYAKCMGHVVDKIFTSLSLGLGLEGNVLREAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFKDNSWFNANYIPNALIVHIGDQIEILSNGKYKGVLHRTTVNKEKTRMSWRCS
>evm.model.ctg000515_pilon_pilon.613
MSKDTILSEFIRSEKEQPAITTYNSPVPEIPAIDLSDPDQEKLVHSITEASIESGIFQVVNHRIPIDVLSKLQRVGREFFE
>evm.model.ctg000270_pilon_pilon.143
MEVERVQAIASFSTMMDTIPSEFIRLEHERPGTTTFHGPVPEMPIIDLSDQDQDNLVRLITKASEESGIFQVVNHGIPVDVINKLQKVGREFFELPQEEKEACATPPGAGHSEGYGTKLQKEIEGKKAWVDHLFNKIWPPSSINYNFWPKSPPSYREANEEYAKCLRGVVDKLLMTLSLGLGLEGNVLKEAVGGDGVEYLLKINYYPPCPRPDLALGVVAHTDMSAITVLVPNEVPGLQVFKDEHWFDAKYTPNALIIHIGDQIEILSNGKYKSVIHRTTVNKEKVRMSWPVFCSPPGDRIIGPLPQFVSDENPAKYKTKKYKDYQYCKINKLPQ
>evm.model.ctg000270_pilon_pilon.144
MEVERVQAVASLSMLMDTIPSEFIRSEHERPGTTTFHGPVPELPAIDLSDQDQDNLVRLITKASEEFGIFQVVNHGIPVDIINKLQKVGREFFELPQEEKEAYATPPGAGHLEGYGTKLQKEIEGKKAWVDHLFNNIWPPSSINYNFWPKNPPSYKANEEFAKCLRGVVDKLLMNLSLGLGLEGNELKEAVGGDGLEYLLKINYYPTCPRPDLALGVVAHTDMPAITVLVPNEVPNDVPGLQVFKDEHWFEAKYIPNALIIHIGDQILSNGKYKSVLHRTTVNKEKVRMSWPVFCSPPGEQVIGPLPQLVSHDDNPAKYKTKKYKDYMYCKINKLPQ
>evm.model.ctg000270_pilon_pilon.168
MEVERVQAIASLSMRKNTIPLEFIRSEKEQPAITTFHGPVPEIPTIDLSDPDQEKLARAISDASQNWGLFQVVNHGIPSDVISKLQSVGRDFFELSHEEKEAYAKPPGAKTIEGYGTKLQKEVEGKKSWVDHLFHKIWPPSSINYHFWPKNPSSYREANEEYAKWLRGVVDKLFTSLSLGLGLEGHVLKDAVGGDELEYLMKINYYPPCPRPDLALGVVAHTDMSTITVLVPNEVAGLQVFKDDHWFDAKYIPNALIVHIGDQIEILSNGKYKSVLHRTTVNKEKTRMSWPVFCEPSPELVMGPLPQLVSEENPAKYKAKKYKDYVYCKLNKLPQ
>evm.model.ctg000051_pilon_pilon.109
MDTIPSEFIRLEHERPGTTTSHGPVPEMPIIDLSDQDQDNLEFGIFQVVNHGIPVDVISKLQKVGREFFELPQEEKEAWLWQKEIEGKKAWVDHLFNKIWPPSSINYNFWPPSYRFKQWVVYLLKINYYPPCPRPDLALALGVVAHTDMSAITVLVPNEVPGLQVFRDEHWFDAKYTPNALIIHIGDQIEILSNGKYKSVLHRTTVNKEKVRMSWPVFCSPPGDRIIGPLPQLVSDDNPAKYKTKKYKLRLSVL
MEIERVQAIALLSMSNDTIPLEFIRSEKEQPAITTFHGPVPEIPTIDLSDSDEEKIVPLIDSTCREWGIFQAVNHGIPSDVISKLQSVGREFFELPQEEKEAYAKPPGSETVEGYGTKLQKEVEGKRAWVDHLFHNVWPPSRINYQFWPKNPPSYRFV
>evm.model.ctg000276_pilon_pilon.79
MEIERVQAIALLSMSNDTIPLEFIRSEKEQPAITTFHGPVPEIPTIDLSDPDEEKIVRLIDSACREWGIFQAVNHGIPSDVMSKLQSVGREFFELPQEEKEAYAKPPGSETVEGMELSFRRKEANEEYTKCMRGVVDKLFRSLSLGLGLEGHVVKEAVGGEELEYLMKINYYPPCPRPDLALGVVAHTDMCTFTVLVPNEVPGLQVFKDDRWFDAKYIPNALIVHIGDQIEILSNGRYKSVLHRTTVNKEKTRMSWPVFCQPPPEVTVGPLSQLVNEENPTKYKTKKYKDYMYCKLNKLPQ
>evm.model.ctg000515_pilon_pilon.285
MSMDTIPSEFIRSEKEQPAITTPAIDLSDPDQEKLVHSIAEASMEWGIFQVVNHGIPIDVVSELQSVGREFFELSEEEKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYREANEEYAKCMGHVVDKIFTSLSLGLGLEGNVLREAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEILSNGKYKGVLHRTTVNKEKTRMSWPVFFELPSELVVGPLPQLVNEDNPAKYKTKKYKEYVHCKLNKHPQ
>evm.model.ctg000515_pilon_pilon.287
MRSFTVATAFAWWPSSADLAAFFAKASSIGSLVLRLQTKAAYMFSMATLACDKYHVPPLFLKDGSRLNPRRLDAFLGLRRRRWWSRDPSEGNLLQDVGQEFIKLPQEEKKYANDPSSGKFKGYGTKMTRNHDEKVEWVDYYFHLMNHAIWPQKPASYREVTEEYAEELLKVTDVLLELLSEGLGLERKALKSSLRGEEIKLDMKINMYPPCPQPQLALGVEPHTDMSALTILIPNDVPGLQVWKDDNWVVVDYKPNVLFIHVVDQIEVGIEISPAIGEELEEEMADDNPIP
>evm.model.ctg000515_pilon_pilon.288
MEVERVQTLSSGGLDELPVQFVRPAHERPENSKAVEGISVPVISLSQPAEILVKEIYNACSQWGFFLVTEHGISPSLICRLQDVGQEFFKLPQGEKETYANDPSSGKFEGYGTKMTRNPDEKVEWIDYYFHLMSPTSKVNHAIWPQKPASYREVTEEYAEELLKVTDVLLELLSEGLGLERKALKSNLRGEEIELEMKINMYPPCPQPQLALGVEPHTDMSALTILIPNDVPGLQVWKDDNWIAVDYIPNALFIHIGDQIEVLSNGKYKSVLHRSLVNKDRMRMSWAVFCTPPLDASIGPLPQLVNDENPSKYSTKTYEEYRYRKFNKIPQEKVWVKKSDQSGTESDIKFAHEITADQHQGKDIDGSGRPQGNEVLTRRINLIEENDKEGYPNEGKE
>evm.model.ctg000515_pilon_pilon.290
MGVERVQAIASLSMSMDTIPSEFIRSEKEQPARTTYNGPVPEIPAIDLTSYSGSDESSSNSQKKKKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYREANEEYAKCMGHVVDKIFTSLSLGLGLEGNVLREAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFKDNSWFNANYIPNALIVHIGDQIEEKTRMSWPVFFEPPSELVVGPLPQLVNEENPAKYKTKKYKEYVHCKLNKLPQ
>evm.model.ctg000515_pilon_pilon.291
MGVERVQAIASLSMSMDTIPSEFIRSEKEQPARTTYNGPVPEIPAIDLSNPDREKLVHSIAEASMEWGIFQVVNHGIPIDVVSELQRVGREFFELSEEEKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYRFVLGQEANEEYAKCMGHVVDKIFTSLSLEAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFKDNSWFNANYIPNALIVHIGDQIEILSNGKYKGVLHRTTVNKEKTRMSWPVFFEPPSELVVGPLPQLVNEDNPAKYKTKKYKEYVHCKLNKLPQ
>evm.model.ctg000515_pilon_pilon.292
MGVERVQAIASLSMSMDTIPSEFIRSEKEQPARTTYNGPVPEIPAIDLSDPDQEKLVHSIAEASMEWGIFQVVNHGIPIDVVSELQRVGREFFELSEEEKEAYAKPPGAQTVEGYGTMLQKEVEGKKAWVDHLFHKIWPPPSINYQFWPKNPPSYREANEEYAKCMGHVVDKIFTSLSLGLGLEGNVLREAAGGNELEYLLKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQVFKDNSWFNANYIPNALIVHIGDQIEILSNGKYKGVLHRTTVNKEKTRMSWRCS
>evm.model.ctg000515_pilon_pilon.613
MSKDTILSEFIRSEKEQPAITTYNSPVPEIPAIDLSDPDQEKLVHSITEASIESGIFQVVNHRIPIDVLSKLQRVGREFFE
>evm.model.ctg000270_pilon_pilon.143
MEVERVQAIASFSTMMDTIPSEFIRLEHERPGTTTFHGPVPEMPIIDLSDQDQDNLVRLITKASEESGIFQVVNHGIPVDVINKLQKVGREFFELPQEEKEACATPPGAGHSEGYGTKLQKEIEGKKAWVDHLFNKIWPPSSINYNFWPKSPPSYREANEEYAKCLRGVVDKLLMTLSLGLGLEGNVLKEAVGGDGVEYLLKINYYPPCPRPDLALGVVAHTDMSAITVLVPNEVPGLQVFKDEHWFDAKYTPNALIIHIGDQIEILSNGKYKSVIHRTTVNKEKVRMSWPVFCSPPGDRIIGPLPQFVSDENPAKYKTKKYKDYQYCKINKLPQ
>evm.model.ctg000270_pilon_pilon.144
MEVERVQAVASLSMLMDTIPSEFIRSEHERPGTTTFHGPVPELPAIDLSDQDQDNLVRLITKASEEFGIFQVVNHGIPVDIINKLQKVGREFFELPQEEKEAYATPPGAGHLEGYGTKLQKEIEGKKAWVDHLFNNIWPPSSINYNFWPKNPPSYKANEEFAKCLRGVVDKLLMNLSLGLGLEGNELKEAVGGDGLEYLLKINYYPTCPRPDLALGVVAHTDMPAITVLVPNEVPNDVPGLQVFKDEHWFEAKYIPNALIIHIGDQILSNGKYKSVLHRTTVNKEKVRMSWPVFCSPPGEQVIGPLPQLVSHDDNPAKYKTKKYKDYMYCKINKLPQ
>evm.model.ctg000270_pilon_pilon.168
MEVERVQAIASLSMRKNTIPLEFIRSEKEQPAITTFHGPVPEIPTIDLSDPDQEKLARAISDASQNWGLFQVVNHGIPSDVISKLQSVGRDFFELSHEEKEAYAKPPGAKTIEGYGTKLQKEVEGKKSWVDHLFHKIWPPSSINYHFWPKNPSSYREANEEYAKWLRGVVDKLFTSLSLGLGLEGHVLKDAVGGDELEYLMKINYYPPCPRPDLALGVVAHTDMSTITVLVPNEVAGLQVFKDDHWFDAKYIPNALIVHIGDQIEILSNGKYKSVLHRTTVNKEKTRMSWPVFCEPSPELVMGPLPQLVSEENPAKYKAKKYKDYVYCKLNKLPQ
>evm.model.ctg000051_pilon_pilon.109
MDTIPSEFIRLEHERPGTTTSHGPVPEMPIIDLSDQDQDNLEFGIFQVVNHGIPVDVISKLQKVGREFFELPQEEKEAWLWQKEIEGKKAWVDHLFNKIWPPSSINYNFWPPSYRFKQWVVYLLKINYYPPCPRPDLALALGVVAHTDMSAITVLVPNEVPGLQVFRDEHWFDAKYTPNALIIHIGDQIEILSNGKYKSVLHRTTVNKEKVRMSWPVFCSPPGDRIIGPLPQLVSDDNPAKYKTKKYKLRLSVL
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University