Enzyme
Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3',7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
ANAT
Enzyme
Anthocyanin acyltransferase
Enzyme name
EC:2.3.1.215
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6''-O-acyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-beta-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [RN:R10237]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-beta-D-glucoside] [CPD:C20461]
Product
Species information
Striga asiatica
Species name
Witchweed (Striga asiatica) is a species of eudicot in the family Orobanchaceae.
Species info
Gene sequence info
Striga_asiatica.ANAT.fa
Download
>SGA_v2.0_scaffold34T13910
MTTILEISEVSPPPGGPTELFVPLTLFDIMWVHFHRIQRLLFYDHPSSQPYFSENLIPNLKRSLSLALQHYLPLSGHLIYPLNTAHDDDKKPVFRYRVGDSVSFTTALSEHDFDELVADNAQDADRFYEFVPEMPPPKDELDCKVVPVLALKATLFPGRGVCIGFSNHHSIGDACSIFAFLKAWSSIAKSKGVDFSFSFSTFDRCFVEDPSGVGNIYWKSMREIALKPRSFPLPTNRVRATFTLDRANIERLKDLVSAEKPSLGRVSSFVVTVSYVRACLAKSSDEIGEETDDDVVDYLIFAVDLRARMDPPVPDNYFGNCLGFGLAKIEHRQLVGGKGFMILAEAVAKDIKNRLNNKHEVLRGAENWISNIRELKGSKSTRVVGVSGSPKFDFSDVDFGWGKARKLEVVSIDGDHYSISLSKSRGSNGGLEVGLSLPRDRMEAFGAIFAKGLMGS
>SGA_v2.0_scaffold49T18314
MTAFIEICRVGPPPDSAAKCSLPLTYQDIFWLHFHPIRRLLFYDLSCSKPEFSDNLVPKLKHSLARVLKQYFPLAGKIVYPSDTSEEDKPVIRFEPGDSVPLAIFESNDDFDLLVGNQPREADSFYDYIPQLPDIAIDSSGRKLLKLLALQVTLFPGRGVCIGIANHHAAGDASSIVGFIRAWASTCKLDRARESHEDYSLPVFDRSMIKDPRGFGSFIWNRAKRNQLTLSPFPLPTKRVRATYIVSRAQIEKLKDLVSAEVPSFVHLSSFVVTSAYAWTCLAKSLSAVEEEEKSRVGGNDDVEFFMFTADIRARVDPPVPGNYFGNFLSGGLGRARNGELVGPGGFVLAARVIAEQVKHVVNDKDRVLERLEDRIEEIGSIVGKRVFSVSGSNRVDLYGADFGFGRARKVETLSIDGEKYAMSICKPRDFEGGLEIGLSLPKAVMDVFAGLFSDGLKP
>SGA_v2.0_scaffold49T18315
MTTLIETSKVGPLPAVAPAAHRTLPLTFLDLCWLHFHPIRRLFFYDCSFSKSHFLESFVPKLKHSLSLALELYFPLAGTLIYPSDTENHKPTIRYSPGDSIPFSIHESSEAGEIFDSLVGNQPRHADAFYDYTPQLPQIVTEPDRKLLRLLAVQVTLFPGRGVCIGLTNHHSAGDASSIVGFVRAWASTCKSGLLENARPVFDRGLIDDPLGRLESVYWDQMRRVPLTLSPFPLPTNRVRATYILDRARIEKLKDWVGAKSGFSGLDRPSSFVVASAHVWTCLAKSLVWDDKNEENRVCDDDDDDEVDYFLFAADIRARLSPPLPGSYFGNCLSGGLAKVRHEELIGPNGLFVAARAIADEIKNKLNGKDRIMGWLENIGKEIMSIGGKRMFSVSGSARVDLYGADFGLGRARKVEALSIDGEKYSMSLCRPRDCEDGLEIGLSLPKDKMDVFASLFDDGLE
>SGA_v2.0_scaffold70T23454
MFRLRQAGPPSYSCPSHSWTSNGSTSIPCETFSFTSTLFRNLATSPKASSRISNDHSPTLSPVIGPFGLPYNRQQQNPLFVTWWETRFHSRLPCRDKISTSSSRITLETPIDSTILLLMWRHRRESDFVSRPRYMHRCFHSSNSAFRMPILDGERRESLRLCRCDGEMYSMSLCESMVSNGGLEIGLSLPRDRMEAFGAIFAKGINGSSRRFGLGFGFGLSTVSPL
>SGA_v2.0_scaffold83T26435
MKALCIRPHRRRVCLQFHRRELLIPHLLLLITCLRTISPHPKSPLYINPNTRPLNFNLQLIFPCILVQAQCVYHHHRHHQSPQSWGRELDCWAIKAWSSLAKLDHGENIKVRENENLPPPRVYNISLNKDLLGLVDVYLNQMRALKLALPPLTSTANKVRVTLVLQKNEIKALQNSLSARYSALPHLLWACLTQAVGSSMTTSSNTLGSPSTVAAAWSCPSWLLIANKMNKKEELLRDADEWLVKYWPLIGKRAFGFSGSLRFDLYDGADFWLGKPAKYEAVLIDGDRSMTLCKSRDFKGGLEIERGRIYLHGYKGNPTSSNQVDPDYLVSSRKLEKWYKNVDEGFVFFGDTAQTIARGIDFCFEDIRSLFYTEFYMKSRNGDFRGRREKGHISDIFCLSQNFRTYTGVLRLAHSVIDTICHFFPLSIDVLPPETSLIYGESPVVLEPRSDENLIMTIFGHSANAGRKWVGFGADQ
>SGA_v2.0_scaffold95T28892
MSTEKLTVISRSAVAPPDGSAGEHVLPLTYFDVVWIYYHPIQRLLFYPYPCSTDIFLQKIAPNLKNSLSKTLAHYLPLAGNLIVRRSSDRPELRYSQEDSVQVTFAESNGDLDFSQLTGNNPRDSNQFHVLVPDLPNPKSESGFDKIPVLAVQVTLFPEAGIAIGITNHHAVGDASSIVGFIKFWSSLAKLGDGDNVKVSENENLPPPPVYDRSLIKDPSGRADLYLNQMRALKFGPIPLTSATNKVRATFVLQKNEIEALRNSLSARYHSLAHLSSFTVTAAYVWACLTQADGPINDDEPEFFGFAVDTRSRLYPPLPASYFGNCLAFAFVEIKHGELRSENGFFTAGKIIGELIANKVKKKEELLRDADEWLVKCWPLIGKRVFSVSGSPRFDLYDGADFGLGKPVKYEAVSIDGDRSMSLCKSRDFEGGLEIGLSLAREIIDAFGHIFSHGLKMR
>SGA_v2.0_scaffold153T37033
MSGENLTVHSRIAVAPSTGDAAPSTGDATEHRLPLTFFDILWLYFHPIQRLLLYPYPCSTDTFLQKISPNLKNSLSKTLDRYLPLAGNLIVPRNSNLPELLYSPGDSVQVTFAESNGDLDFSSLTGNHPRNSDQFHSLVPDLPDPKHELGFDFDKIPVLAVQITLFPQTGISIGITNHHASGDASSIVRFIKTWSSYANLAAGHEIENENLLPRPIYDRSLIKDPLGLTEIFWAQMRADKIGLSASKALPNKLHAQKFAAAQNPAQAHISSFTVITAYVWACLAKSIGPIENDVPEYFVFAVDARGRVDPPLPEGSENGFFEAAKVIGEAIANKVNNKEELFRGADEWLPSYGLLLGKRLFGVVGSPRFDLYDGADFGLGKPEKYEAVSIDWGGSMSLCKWREFEGGLEIGLSLRKEEMDSFGPIFYDGFVIDGEVAFSVRFAVDQSNVNIIEQ
>SGA_v2.0_scaffold159T37620
MPVFRYASTDSVPVSVFSSSSAHDFDTLVANQPREADVFYDYAPRLPPAVTSPDGTLLQGLLALQVTIFPGRGICIGVANHHAIGDATSIVGFIRDWASTCKLGVVQNSRPIFERDLIKGPPGLDSIYWNNMKRVPFGKSSPFPLPTNRVRATFVLDRARIKKLKDLVVAKFPSFPHLSSFVVTSAYVWTCFAKSLSAGEENENRVNDDDDDNEMEFFLFSADIRARLSPPLPESYFGNCLSGGVPKIRHHELIGPDGFFLAARAIADEIKNKLNGMDKITEWFENGPKTIRSVLGKRLFSVSGSARVDLYGADFGLGRARKVETLSIDGEKYSMSLCKPRDCEGGLEVGMSLPKEVMDVFVGLFNDGF
>SGA_v2.0_scaffold159T37616
MAESPAPATVLDLFKVHPSPETAPAPDMTLPLVHFDITWLHFHRVQRLLFFDSPCSKTRFLQSLVPELRRSLAHALARFLPLAGKIVHPLDDAGRPSIRFSPGDSVPLTVAECYSDFDHLTGDHPRLSDEFYPFVPELPPATRSENEVVIPALSLQVTLFPGHGLCLGFTNHHAAGDASSIVRFIKSWAAANKLGTGGASAAELTPYYDRSTVADPDGLDRIYWDLIGKSRAVESPPLTFPLHKLRRTFILTGDHVRLLKAAVLSQLPAARHVTTFTVACALAWSCLLRADPPAADDEPEYMGFAADCRSRLNPPLPAAYFGNCLAFVLAESRHGLLRAGGVEGLAHAAEAIGSAVQRTVYNEKGILDGAEGWPAGYGKLIGKRLFGVAGSPRFDLYDVDFGWGPPKKFESPSIDADTSMSLCKSRDFEGGLEIGLSRPKNVLEAFAANFEEQLRNL
>SGA_v2.0_scaffold159T37613
MTTEGSQHFLRLRPSAPADRHIARRHPSPLFPGRANHHTIGDATSIVGFIRDWAKLGVVPNSRPVFERDLIKVPQGLDSIYWNNMKCVPFGSSSTFPLPTNRVRATFVLDRARFKKLKDLVVAEFSELFPPIVFCRDFGLRLDVRYSGPSQPAIARELLRQLLIGRASEIRHHELIGPDGFFLAARAIADEIKYKLNGMDKITEWFENAPKIIRSVLGKCLFSVSGSVRVDLYGADFGLGRARKMEILSIDGEKYTMSLCKPRDCEGGLEVGMSLTKEVRDVFVGLFNDGLNT
MTTILEISEVSPPPGGPTELFVPLTLFDIMWVHFHRIQRLLFYDHPSSQPYFSENLIPNLKRSLSLALQHYLPLSGHLIYPLNTAHDDDKKPVFRYRVGDSVSFTTALSEHDFDELVADNAQDADRFYEFVPEMPPPKDELDCKVVPVLALKATLFPGRGVCIGFSNHHSIGDACSIFAFLKAWSSIAKSKGVDFSFSFSTFDRCFVEDPSGVGNIYWKSMREIALKPRSFPLPTNRVRATFTLDRANIERLKDLVSAEKPSLGRVSSFVVTVSYVRACLAKSSDEIGEETDDDVVDYLIFAVDLRARMDPPVPDNYFGNCLGFGLAKIEHRQLVGGKGFMILAEAVAKDIKNRLNNKHEVLRGAENWISNIRELKGSKSTRVVGVSGSPKFDFSDVDFGWGKARKLEVVSIDGDHYSISLSKSRGSNGGLEVGLSLPRDRMEAFGAIFAKGLMGS
>SGA_v2.0_scaffold49T18314
MTAFIEICRVGPPPDSAAKCSLPLTYQDIFWLHFHPIRRLLFYDLSCSKPEFSDNLVPKLKHSLARVLKQYFPLAGKIVYPSDTSEEDKPVIRFEPGDSVPLAIFESNDDFDLLVGNQPREADSFYDYIPQLPDIAIDSSGRKLLKLLALQVTLFPGRGVCIGIANHHAAGDASSIVGFIRAWASTCKLDRARESHEDYSLPVFDRSMIKDPRGFGSFIWNRAKRNQLTLSPFPLPTKRVRATYIVSRAQIEKLKDLVSAEVPSFVHLSSFVVTSAYAWTCLAKSLSAVEEEEKSRVGGNDDVEFFMFTADIRARVDPPVPGNYFGNFLSGGLGRARNGELVGPGGFVLAARVIAEQVKHVVNDKDRVLERLEDRIEEIGSIVGKRVFSVSGSNRVDLYGADFGFGRARKVETLSIDGEKYAMSICKPRDFEGGLEIGLSLPKAVMDVFAGLFSDGLKP
>SGA_v2.0_scaffold49T18315
MTTLIETSKVGPLPAVAPAAHRTLPLTFLDLCWLHFHPIRRLFFYDCSFSKSHFLESFVPKLKHSLSLALELYFPLAGTLIYPSDTENHKPTIRYSPGDSIPFSIHESSEAGEIFDSLVGNQPRHADAFYDYTPQLPQIVTEPDRKLLRLLAVQVTLFPGRGVCIGLTNHHSAGDASSIVGFVRAWASTCKSGLLENARPVFDRGLIDDPLGRLESVYWDQMRRVPLTLSPFPLPTNRVRATYILDRARIEKLKDWVGAKSGFSGLDRPSSFVVASAHVWTCLAKSLVWDDKNEENRVCDDDDDDEVDYFLFAADIRARLSPPLPGSYFGNCLSGGLAKVRHEELIGPNGLFVAARAIADEIKNKLNGKDRIMGWLENIGKEIMSIGGKRMFSVSGSARVDLYGADFGLGRARKVEALSIDGEKYSMSLCRPRDCEDGLEIGLSLPKDKMDVFASLFDDGLE
>SGA_v2.0_scaffold70T23454
MFRLRQAGPPSYSCPSHSWTSNGSTSIPCETFSFTSTLFRNLATSPKASSRISNDHSPTLSPVIGPFGLPYNRQQQNPLFVTWWETRFHSRLPCRDKISTSSSRITLETPIDSTILLLMWRHRRESDFVSRPRYMHRCFHSSNSAFRMPILDGERRESLRLCRCDGEMYSMSLCESMVSNGGLEIGLSLPRDRMEAFGAIFAKGINGSSRRFGLGFGFGLSTVSPL
>SGA_v2.0_scaffold83T26435
MKALCIRPHRRRVCLQFHRRELLIPHLLLLITCLRTISPHPKSPLYINPNTRPLNFNLQLIFPCILVQAQCVYHHHRHHQSPQSWGRELDCWAIKAWSSLAKLDHGENIKVRENENLPPPRVYNISLNKDLLGLVDVYLNQMRALKLALPPLTSTANKVRVTLVLQKNEIKALQNSLSARYSALPHLLWACLTQAVGSSMTTSSNTLGSPSTVAAAWSCPSWLLIANKMNKKEELLRDADEWLVKYWPLIGKRAFGFSGSLRFDLYDGADFWLGKPAKYEAVLIDGDRSMTLCKSRDFKGGLEIERGRIYLHGYKGNPTSSNQVDPDYLVSSRKLEKWYKNVDEGFVFFGDTAQTIARGIDFCFEDIRSLFYTEFYMKSRNGDFRGRREKGHISDIFCLSQNFRTYTGVLRLAHSVIDTICHFFPLSIDVLPPETSLIYGESPVVLEPRSDENLIMTIFGHSANAGRKWVGFGADQ
>SGA_v2.0_scaffold95T28892
MSTEKLTVISRSAVAPPDGSAGEHVLPLTYFDVVWIYYHPIQRLLFYPYPCSTDIFLQKIAPNLKNSLSKTLAHYLPLAGNLIVRRSSDRPELRYSQEDSVQVTFAESNGDLDFSQLTGNNPRDSNQFHVLVPDLPNPKSESGFDKIPVLAVQVTLFPEAGIAIGITNHHAVGDASSIVGFIKFWSSLAKLGDGDNVKVSENENLPPPPVYDRSLIKDPSGRADLYLNQMRALKFGPIPLTSATNKVRATFVLQKNEIEALRNSLSARYHSLAHLSSFTVTAAYVWACLTQADGPINDDEPEFFGFAVDTRSRLYPPLPASYFGNCLAFAFVEIKHGELRSENGFFTAGKIIGELIANKVKKKEELLRDADEWLVKCWPLIGKRVFSVSGSPRFDLYDGADFGLGKPVKYEAVSIDGDRSMSLCKSRDFEGGLEIGLSLAREIIDAFGHIFSHGLKMR
>SGA_v2.0_scaffold153T37033
MSGENLTVHSRIAVAPSTGDAAPSTGDATEHRLPLTFFDILWLYFHPIQRLLLYPYPCSTDTFLQKISPNLKNSLSKTLDRYLPLAGNLIVPRNSNLPELLYSPGDSVQVTFAESNGDLDFSSLTGNHPRNSDQFHSLVPDLPDPKHELGFDFDKIPVLAVQITLFPQTGISIGITNHHASGDASSIVRFIKTWSSYANLAAGHEIENENLLPRPIYDRSLIKDPLGLTEIFWAQMRADKIGLSASKALPNKLHAQKFAAAQNPAQAHISSFTVITAYVWACLAKSIGPIENDVPEYFVFAVDARGRVDPPLPEGSENGFFEAAKVIGEAIANKVNNKEELFRGADEWLPSYGLLLGKRLFGVVGSPRFDLYDGADFGLGKPEKYEAVSIDWGGSMSLCKWREFEGGLEIGLSLRKEEMDSFGPIFYDGFVIDGEVAFSVRFAVDQSNVNIIEQ
>SGA_v2.0_scaffold159T37620
MPVFRYASTDSVPVSVFSSSSAHDFDTLVANQPREADVFYDYAPRLPPAVTSPDGTLLQGLLALQVTIFPGRGICIGVANHHAIGDATSIVGFIRDWASTCKLGVVQNSRPIFERDLIKGPPGLDSIYWNNMKRVPFGKSSPFPLPTNRVRATFVLDRARIKKLKDLVVAKFPSFPHLSSFVVTSAYVWTCFAKSLSAGEENENRVNDDDDDNEMEFFLFSADIRARLSPPLPESYFGNCLSGGVPKIRHHELIGPDGFFLAARAIADEIKNKLNGMDKITEWFENGPKTIRSVLGKRLFSVSGSARVDLYGADFGLGRARKVETLSIDGEKYSMSLCKPRDCEGGLEVGMSLPKEVMDVFVGLFNDGF
>SGA_v2.0_scaffold159T37616
MAESPAPATVLDLFKVHPSPETAPAPDMTLPLVHFDITWLHFHRVQRLLFFDSPCSKTRFLQSLVPELRRSLAHALARFLPLAGKIVHPLDDAGRPSIRFSPGDSVPLTVAECYSDFDHLTGDHPRLSDEFYPFVPELPPATRSENEVVIPALSLQVTLFPGHGLCLGFTNHHAAGDASSIVRFIKSWAAANKLGTGGASAAELTPYYDRSTVADPDGLDRIYWDLIGKSRAVESPPLTFPLHKLRRTFILTGDHVRLLKAAVLSQLPAARHVTTFTVACALAWSCLLRADPPAADDEPEYMGFAADCRSRLNPPLPAAYFGNCLAFVLAESRHGLLRAGGVEGLAHAAEAIGSAVQRTVYNEKGILDGAEGWPAGYGKLIGKRLFGVAGSPRFDLYDVDFGWGPPKKFESPSIDADTSMSLCKSRDFEGGLEIGLSRPKNVLEAFAANFEEQLRNL
>SGA_v2.0_scaffold159T37613
MTTEGSQHFLRLRPSAPADRHIARRHPSPLFPGRANHHTIGDATSIVGFIRDWAKLGVVPNSRPVFERDLIKVPQGLDSIYWNNMKCVPFGSSSTFPLPTNRVRATFVLDRARFKKLKDLVVAEFSELFPPIVFCRDFGLRLDVRYSGPSQPAIARELLRQLLIGRASEIRHHELIGPDGFFLAARAIADEIKYKLNGMDKITEWFENAPKIIRSVLGKCLFSVSGSVRVDLYGADFGLGRARKMEILSIDGEKYTMSLCKPRDCEGGLEVGMSLTKEVRDVFVGLFNDGLNT
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University