Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Striga asiatica
Species name
Witchweed (Striga asiatica) is a species of eudicot in the family Orobanchaceae.
Species info
Gene sequence info
Striga_asiatica.COMT.fa
Download
>SGA_v2.0_scaffold7T03645
MGSNSEKTSNPAARSLPNDNDEEAGVFAMQLATGSVLPMVLRAAVELDLLELIKQAEPAASASELAARLPTKNPDAAAMVDRILRFLVAHGVLTCTTEGG
>SGA_v2.0_scaffold16T07641
MAEPFSCHETFFFAMQLASAFVLPMVLKSAIELNLLELIWRADMIKIARLDAQFCLKGYEDCLLDDRLLQGCLDTSFLATISVQQDRLEVDIHMELKNKEQMNFQFNKVCLVFLVSKSNPCCPCTCVLVAPSVPAACCSRAGLARVTCTSRASPAAHAFSSSGSPSVHNAQRAHASSTPVRVRLPAHACAQHASRTRLAHPVFCASHLVPWPPPLGLPPFTCIPKPPMERLSRGVLGSRPLDVLGGQNLHSHHDGGAWFSPMNLAAQLPTANSEASVMLDRILCLLVRYSILNCRLRALSDSGTTWKSIFFV
>SGA_v2.0_scaffold18T08471
MQSPAIENLGASKTADRISGEEEEEGCLLAMQLASAYGELGAQKECYFERYHLKDAVLEGGIPFNKAYGMTAFEYHGKDQRSTIQKFSFISTSIEHVGGDMFASMPKGDAIFMKWICHDWSEEYCLKFLKNCYEALPGNGKVILAECILRRLRIQSLPRKLLSMWTVLLSLFSIVYHTTSLGIRSQHDSRCSSDDKIQSTGVTPPSTIGLSRWMIDFSRWTIDSLIYQWISNNWVSLSYHGYVDFEELRRREPRRETEGFGESQWETSSWVEHLNGDTSDFYDGPLAYNEEPPGSEENLTEDVGGSRALFKEDTGFYDGPPVFDEETLKTEERSGAPTPVAGGSSCTWIPALGTGSQPVGGLQCFFCGELGRRQVSYPTPSFSLVLLAGDSGAAEYDELSMFGEEQSVTEEHVCGDVGPLKYMTLFYLQMVSWDYTNVLSNNGGQFHLKSAFACGTAVDDDNEIESDSMANLAQSGENDAE
>SGA_v2.0_scaffold44T16851
MGSTAKNIGGSTMADPSSGEEEAFLFAMQLASASVLPMVLKSAIELDLLELIRRAGDGAWVSPADLAAQLPTANPEANVMLDRILRLLATYSVLNCRLRPLPDGGTERLYGLAPVCKFLTRNEDGVSMAALLLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDLRFNKVFNQGMSNHSTIVMKKILEIYDGFVGLETVVDVGGGTGATLDMIVSKYPSIKGINFDLPHVVDDAPSYPGIKHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYEALPEKGKVILAECILPEAPDTGLATKNVVHIDVIMLAHNPGGKERTEKEFQTLAKGAGFKGFNKVCCAYNSWIMELLK
>SGA_v2.0_scaffold141T35564
MDEKISNPAARSTPEDDEEAGVFAMQLAAGSVLPMVLRAAIELDLLELIKQAEPAASASELAARLPTKNPDAAAMVDRILRFLAAHGVLTCTMEGGQRRYGLEAWARRYLTRNEDGVSLAACYSLMIQDRVFMETWYHLKDAVLEGGVAFDRAYGMNAFEYPGTDPRFNQVFNQGMFEQSTIFMKKILEKYKGFEGLKTLVDVGGGIGASLKMIVSKYPSMRGINFDLPHVIKEAPSYPGVEHIGGDMFVSVPKADAMFMKWICHDWSDVHCKKLLKNCYEALPHDGKVIIADFIFPEDHNSGLAYARAALSDMMMLAYNPGGKERTYKEFQALADYAGFKRSIKVCSAYNIWIMEFHK
>SGA_v2.0_scaffold160T37722
MEVTHNWSDEQWVKLLKNCYDAIPANGKVILVELVLPEGPDYAGVATQNALSKDLMMMCFTQGGKERTETEFEKLAMDAGFNDFKKAACAYNYWIMELRK
>SGA_v2.0_scaffold165T38194
MANSSSLPSDEESYLFAIRLVTASILPAVLKCAVELDLLEIIKREGSASAAELAAQIGSRNPQATAMLDRILSLLAAAGVLECCLAGDGAQRRYMLAPVCKLLTRNEDGASLAPLLLMNQDRPFVETWFHLKDAIVEGGMPFERAHGMSAFEYPCKDPRFNKVFNEAMGQQSTLFMNKLLEVYRGFEGLNSLVDVGGGVGASLRMIVSKYPSIKGINFDLSHVIQNASPHPGVEHVSGDMFVSVPKGDAILMKWICHDWSDSQCVKLLRNCCEAVPAEKGKVIIVDLILPEMPINTDLYSYIGFTVDMSMMTYNPGGKERTEKEFQALAKQSGFKDFRKVCSTFGTWIMELYK
>SGA_v2.0_scaffold222T42790
MDYSAVVNEDDAHLLATHIAACSAFPAAVKCAIELNLFELMKQLAGFVSASELAAKVAPANSEAHLMIDRILRLLASYSVVKCRHTNNERHYSLGPVCEFFTDNQDGASLAPLFLLMHANLYQTSWGKLKDSIVEGGVPFNKAHGMSLYEHSALNPKSNKLFFDAMSDNSTILMKKIVATYNGFEGLNSIVDVGGGIGSTTNMIVSRYPMIKGINFDLLHVIQHAPSYSGKLHMYLLSLVFLIYFITYLERDQQYIYLLR
>SGA_v2.0_scaffold222T42789
MLRGVEHIAGDMFVNVPKADAIFLKEVTHNWSDEQWVKLLKNCYDAIPANGKVILVELVLPEGPDYAGVATQNALSKDLMMMCFTQGGKERTETEFENLFCLPPAFLAQFTLRFLAAAATDGRTPFLRSFCSSLFSSCPRPRFSFFPFVTVMVLSPGNKDSKGPALLALAVQTVPKTCPSSVVAFGRFFKKRKKKDFEVKEGCSLMVSPSTIAGGVASQNFARVETLTGEGEVRHISSAQLLSAGKPSSCSDVFLR
MGSNSEKTSNPAARSLPNDNDEEAGVFAMQLATGSVLPMVLRAAVELDLLELIKQAEPAASASELAARLPTKNPDAAAMVDRILRFLVAHGVLTCTTEGG
>SGA_v2.0_scaffold16T07641
MAEPFSCHETFFFAMQLASAFVLPMVLKSAIELNLLELIWRADMIKIARLDAQFCLKGYEDCLLDDRLLQGCLDTSFLATISVQQDRLEVDIHMELKNKEQMNFQFNKVCLVFLVSKSNPCCPCTCVLVAPSVPAACCSRAGLARVTCTSRASPAAHAFSSSGSPSVHNAQRAHASSTPVRVRLPAHACAQHASRTRLAHPVFCASHLVPWPPPLGLPPFTCIPKPPMERLSRGVLGSRPLDVLGGQNLHSHHDGGAWFSPMNLAAQLPTANSEASVMLDRILCLLVRYSILNCRLRALSDSGTTWKSIFFV
>SGA_v2.0_scaffold18T08471
MQSPAIENLGASKTADRISGEEEEEGCLLAMQLASAYGELGAQKECYFERYHLKDAVLEGGIPFNKAYGMTAFEYHGKDQRSTIQKFSFISTSIEHVGGDMFASMPKGDAIFMKWICHDWSEEYCLKFLKNCYEALPGNGKVILAECILRRLRIQSLPRKLLSMWTVLLSLFSIVYHTTSLGIRSQHDSRCSSDDKIQSTGVTPPSTIGLSRWMIDFSRWTIDSLIYQWISNNWVSLSYHGYVDFEELRRREPRRETEGFGESQWETSSWVEHLNGDTSDFYDGPLAYNEEPPGSEENLTEDVGGSRALFKEDTGFYDGPPVFDEETLKTEERSGAPTPVAGGSSCTWIPALGTGSQPVGGLQCFFCGELGRRQVSYPTPSFSLVLLAGDSGAAEYDELSMFGEEQSVTEEHVCGDVGPLKYMTLFYLQMVSWDYTNVLSNNGGQFHLKSAFACGTAVDDDNEIESDSMANLAQSGENDAE
>SGA_v2.0_scaffold44T16851
MGSTAKNIGGSTMADPSSGEEEAFLFAMQLASASVLPMVLKSAIELDLLELIRRAGDGAWVSPADLAAQLPTANPEANVMLDRILRLLATYSVLNCRLRPLPDGGTERLYGLAPVCKFLTRNEDGVSMAALLLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDLRFNKVFNQGMSNHSTIVMKKILEIYDGFVGLETVVDVGGGTGATLDMIVSKYPSIKGINFDLPHVVDDAPSYPGIKHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYEALPEKGKVILAECILPEAPDTGLATKNVVHIDVIMLAHNPGGKERTEKEFQTLAKGAGFKGFNKVCCAYNSWIMELLK
>SGA_v2.0_scaffold141T35564
MDEKISNPAARSTPEDDEEAGVFAMQLAAGSVLPMVLRAAIELDLLELIKQAEPAASASELAARLPTKNPDAAAMVDRILRFLAAHGVLTCTMEGGQRRYGLEAWARRYLTRNEDGVSLAACYSLMIQDRVFMETWYHLKDAVLEGGVAFDRAYGMNAFEYPGTDPRFNQVFNQGMFEQSTIFMKKILEKYKGFEGLKTLVDVGGGIGASLKMIVSKYPSMRGINFDLPHVIKEAPSYPGVEHIGGDMFVSVPKADAMFMKWICHDWSDVHCKKLLKNCYEALPHDGKVIIADFIFPEDHNSGLAYARAALSDMMMLAYNPGGKERTYKEFQALADYAGFKRSIKVCSAYNIWIMEFHK
>SGA_v2.0_scaffold160T37722
MEVTHNWSDEQWVKLLKNCYDAIPANGKVILVELVLPEGPDYAGVATQNALSKDLMMMCFTQGGKERTETEFEKLAMDAGFNDFKKAACAYNYWIMELRK
>SGA_v2.0_scaffold165T38194
MANSSSLPSDEESYLFAIRLVTASILPAVLKCAVELDLLEIIKREGSASAAELAAQIGSRNPQATAMLDRILSLLAAAGVLECCLAGDGAQRRYMLAPVCKLLTRNEDGASLAPLLLMNQDRPFVETWFHLKDAIVEGGMPFERAHGMSAFEYPCKDPRFNKVFNEAMGQQSTLFMNKLLEVYRGFEGLNSLVDVGGGVGASLRMIVSKYPSIKGINFDLSHVIQNASPHPGVEHVSGDMFVSVPKGDAILMKWICHDWSDSQCVKLLRNCCEAVPAEKGKVIIVDLILPEMPINTDLYSYIGFTVDMSMMTYNPGGKERTEKEFQALAKQSGFKDFRKVCSTFGTWIMELYK
>SGA_v2.0_scaffold222T42790
MDYSAVVNEDDAHLLATHIAACSAFPAAVKCAIELNLFELMKQLAGFVSASELAAKVAPANSEAHLMIDRILRLLASYSVVKCRHTNNERHYSLGPVCEFFTDNQDGASLAPLFLLMHANLYQTSWGKLKDSIVEGGVPFNKAHGMSLYEHSALNPKSNKLFFDAMSDNSTILMKKIVATYNGFEGLNSIVDVGGGIGSTTNMIVSRYPMIKGINFDLLHVIQHAPSYSGKLHMYLLSLVFLIYFITYLERDQQYIYLLR
>SGA_v2.0_scaffold222T42789
MLRGVEHIAGDMFVNVPKADAIFLKEVTHNWSDEQWVKLLKNCYDAIPANGKVILVELVLPEGPDYAGVATQNALSKDLMMMCFTQGGKERTETEFENLFCLPPAFLAQFTLRFLAAAATDGRTPFLRSFCSSLFSSCPRPRFSFFPFVTVMVLSPGNKDSKGPALLALAVQTVPKTCPSSVVAFGRFFKKRKKKDFEVKEGCSLMVSPSTIAGGVASQNFARVETLTGEGEVRHISSAQLLSAGKPSSCSDVFLR
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University