Enzyme
Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.
5AT
Enzyme
Anthocyanin 5-aromatic acyltransferase
Enzyme name
EC:2.3.1.153
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase
Sysname
4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [RN:R05163]
Reaction
CoA [CPD:C00010];anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside) [CPD:C06363]
Product
Species information
Spinacia oleracea
Species name
Spinach (Spinacia oleracea) is a species of eudicot in the family Chenopodiaceae.
Species info
Gene sequence info
Spinacia_oleracea.5AT.fa
Download
>rna-XM_021996553.1
MNSETTMKQISECFIKPKYEPNEANKPYYLSPMDLAMLSMHYIQKGLLFKKPSKLDHDQEFSIHKLKESLSLTLVHFYPLAGQFVTQVDDDHSLIFIDCNKGPGARFIHSTLDMTISDILSPTYVPVVVHSFFDHDRAVSHDAHSMSLLTVKVTELIDGVFIGCSINHAVVDGTSFWHFWNVWSEIHTSMDLVEQLPLSRMPVHEGWFPEGYDPPSRLPFSQPDEFVSRYEPPQLKERFFHFSSGSMARLKAKANEENNNNNNNNNNNNNNKISSFQALSALCWRAIIRANNLPKHHPTSCKLATNNRHRLTPPLSQHYFGNCVSVINTTTTASELLEHSLGLTAESLHQTVVNHDDKSVRDFFNNWMKSPIVYQLNKLFDDYSVVMSSSPRFDMYGNEFGFGKAVAVRSGYANKSVGSVISYEGYEGGGSVDLEICLSSGLMEALESDEEFMGFVLN
>rna-XM_021987746.1
MGTLYQVPPPKSVQDLKVTILDSCLIFSPKENDVQKKPMFLSNIDQVLNFDVQTIHFFTPNPEFPPKIAFKMLKNALQKLLVPYNFLAGRVKVNQESGRIEVDCENFGVGFVVASSEYKLDELGDLVYPNPSFEQLITKKMDDILEVDGHPLCIVQMTAFKCGGFAMGLQTNHAIFDGTSIKYLLQNLAALAGDKPLAVVPCHDRELLAARSPPRVTFSHPELLKLKIPIGQEGEIEQNCNVYDTTTQDLDFKIFSLSGNDIATLKAKAKEGPTNGNGKITGFNVVSALVWRSKALSNYYCDNNNNNNNQEKKSSTMLFAVDIRPKLNPPLPPSFTGNAVLTAYANTDCEILEKAPLSELVDLVTEGVTRMNDEYARSSIDWGELYKGFPHGEFLMSSWWRLGFNQVEYPWGKPKYCCPLVYHRKDIILLFPDIEESNSGVNVLVTLPHKEMAKFESLFYNFLSS
>rna-XM_021990831.1
MSLTVKESSMVCPAEATPRRSLWLSRLDMIIRMPFSHSNIVGAYSPTADIEGPKCLDTEILKEALSRVLVPFYPMAGRLKENEENGRFEIDCNAEGALFVEVETTHTLAELGDDFREIVIPTCDYSGGLSSFPLVMLQVTRFKCGEVCLGFAAHHHLVDGMSISFFFSSWARLARGLQLQAQPFVDRIDYLAPRNPPEVNFQHLEYDQPLPPILPEGLSGEIDATSRECMFTFTKHDIKTLNLQATYLDSEAQEANSHKLSTYEVLAGHVWRTACKARGLTGDQDVKLYIPTDGRSRLRDPTLPQGYLGNAIFFTACIAKAGDITCKPLRYAASKVQEALKRVQNTEYLRSAIDYLESEPDLSTLVRGAQHVTCPNLVINSWGKIPVDIADFGWGKPKFLVQGGVRHEGQAFLIPKQDQQGGFLLTIKLFNIHIPHFQDCLFDFESIPTD
>rna-XM_021992389.1
MSMLKAKANEECNVVENNISSFQALTGFLWKSITRIRNIHPDEETSCTLIINWRGRVIPPFSQEHFGNYLAVAQGRCKVGELVSRGLGWAALLVHQSVKAENDCKILEFLRNYSKAPYVEQAGKDSEFHNANSVSIAGSARFDMYGPEFGLGKPVAVLAGYGNKDDGKVTANPGCEGGGSVDLEICLRPQTMIGLESHEEFMSFAS
>rna-XM_021992376.1
MMNSHGEISQVKLISQSFVKPKIQVESSNQPHYLGMIDLAFLSLDQMQKGLLFHTKPENIQAHLLDKLKNSLSHSLVHFYPLAGQLATQKFHDEHAIWVYVDCNKGPGARLIHASVNNLKVSDLISSVDVHPIVRSFFDLGEKAVNHDGHTRPLLSIQVTELLDGVFIGFTMNHSIGDGTSLWNFISTLSEILLHLDGNNIGANDEKFNVSISHKPVFKPFYPDGYGPILKLPFLEPNEFITRNVDQGPLRERIFHFSPTSMSKLKVKANKECGLAYNHISSFQALTAFIWISITHARNLELGQETDVGLVANLRPKINPSISGDYFGGFVKRAQGSCKVGELLGQGLGWASILVHEVVMGLDDKAIRAFFKRFVEAPFVVQPSTANLYYKPNSVLIGGSTRFDMYGSEFGLGKAVGVLAGYASKEDGKVTANPGRQGDGSVDLEVCLRPEIMSSLELDEEFMSFVSAV
>rna-XM_021993934.1
MESSIVKKISECYVKPKNEVSESKTPYHVAPLDLIMLSYHYIQDGLLFMKPNSHLNGNNEFSIHSFLESLKQSLSDTLVHFYPLAGQLVVHFDEDQHQCRVFVDCNKGPGARFSHATALDLTVSDILSPNDDIPFVVRSFFDYNEEAVNYEGHSKPLLSVQVTQLIDGVFIACSINHAISDGTSYWHFWNVWSEIHRGSGNNVSISRLPIHNRWFPEGCVLPIPLPFTHPNQFVKKFEPPQLREKMFHFSPESIATLKTKANKESNSSDMISSFQALSALVWRSTVRANDLPHDQVIYNYMFANNRHRLNPPLPQNYFGTCIKGITTKTSAGELLDNNLGWAASLLHQSVLNLTDKVVRDFVKDWLKSPYCYHHTDLHDYNSIAMEHSPRFDMYGNDFGIGKPVAVRNGPGNKAVGVVFAYPGCEGNGSVDFEICLPPHSMKALETDDEFMAAVSLPK
>rna-XM_021995862.1
MERQLKLEVEIVSIETIKPSLPPPSHPKTYTLSLLDQIFPAFHIPVLLFYTSTQQRIDINTLKISLSQTLDRFYPLAGRFLDESTISCNNQGIPLIETKVDCHLKVFMSSPHKLNLLNKFLPSKDLQALGPRPVSELLPLAIQVNKFLCGGLVIGCYMLHKILDISSLGTFFKHWAACASNQHKDLLQLQPDFDAIVKAFPPLPMVEKVFVPEFCSKDTLNVLQHPTIVVKSFTFSTVSLTNLRAKAASELVPRPTRFEAVAGLLWEQTLAAAYTATGIHCLTGEVNNNNNNNNNNNNNNNNNNNNNNNNNNNNTVLSVTVNMRQWMSPPLPSGSMGNLVTNVMAKADKRSRFQELVKVIHSSLSYIKEKITKVYQSENGADILLSERKAESEGVMEYQEGLYRLSSWCRTGLNDADFGFGRPAWILPTDYSNPPPTLRNFMFLTDFADGIEAWLFLEEKEMQLLQSNPHFLAFASPN
>rna-XM_021983693.1
MEFWRVKEISKWFVKPKYSVSESKKPYHLGPLDLAMLSFHYIQNGLLFSKPSHLNGHEFSIDSFLESLKESLSHTLIHFYPLAGQLVIQVDEAKHECCVFVDCNKGPGAQFSHATALDITVADVLSPNNDIPLIVASFFYYNEPEVAVNYDGHFKPLLSVQVTELLDGVFIACSINHAIADGTAYWHFWNVWSEIHRAKDKGYLIPVSRLPIHNRWFPDGCVLPITLPFTRPEEFIRRFDTPQLREKVFHFSSDSINTIRAKANKESTGSSMMISTFQALSALVWRSIVRANCLPHDQILYNYMFASNRHRLNPPLPQNYFGTCIKGITTKTTAGELLKNNLGWAALLLHQSVVSLTDKAVREFVKEWLESPYCYHHRDLHDYNTVAMEHSPRFNMYGNEFGIGKPIAVRGGRGNKAVGVVNAYPGYQGTGSVDLEICLPPDSMRALEKF
MNSETTMKQISECFIKPKYEPNEANKPYYLSPMDLAMLSMHYIQKGLLFKKPSKLDHDQEFSIHKLKESLSLTLVHFYPLAGQFVTQVDDDHSLIFIDCNKGPGARFIHSTLDMTISDILSPTYVPVVVHSFFDHDRAVSHDAHSMSLLTVKVTELIDGVFIGCSINHAVVDGTSFWHFWNVWSEIHTSMDLVEQLPLSRMPVHEGWFPEGYDPPSRLPFSQPDEFVSRYEPPQLKERFFHFSSGSMARLKAKANEENNNNNNNNNNNNNNKISSFQALSALCWRAIIRANNLPKHHPTSCKLATNNRHRLTPPLSQHYFGNCVSVINTTTTASELLEHSLGLTAESLHQTVVNHDDKSVRDFFNNWMKSPIVYQLNKLFDDYSVVMSSSPRFDMYGNEFGFGKAVAVRSGYANKSVGSVISYEGYEGGGSVDLEICLSSGLMEALESDEEFMGFVLN
>rna-XM_021987746.1
MGTLYQVPPPKSVQDLKVTILDSCLIFSPKENDVQKKPMFLSNIDQVLNFDVQTIHFFTPNPEFPPKIAFKMLKNALQKLLVPYNFLAGRVKVNQESGRIEVDCENFGVGFVVASSEYKLDELGDLVYPNPSFEQLITKKMDDILEVDGHPLCIVQMTAFKCGGFAMGLQTNHAIFDGTSIKYLLQNLAALAGDKPLAVVPCHDRELLAARSPPRVTFSHPELLKLKIPIGQEGEIEQNCNVYDTTTQDLDFKIFSLSGNDIATLKAKAKEGPTNGNGKITGFNVVSALVWRSKALSNYYCDNNNNNNNQEKKSSTMLFAVDIRPKLNPPLPPSFTGNAVLTAYANTDCEILEKAPLSELVDLVTEGVTRMNDEYARSSIDWGELYKGFPHGEFLMSSWWRLGFNQVEYPWGKPKYCCPLVYHRKDIILLFPDIEESNSGVNVLVTLPHKEMAKFESLFYNFLSS
>rna-XM_021990831.1
MSLTVKESSMVCPAEATPRRSLWLSRLDMIIRMPFSHSNIVGAYSPTADIEGPKCLDTEILKEALSRVLVPFYPMAGRLKENEENGRFEIDCNAEGALFVEVETTHTLAELGDDFREIVIPTCDYSGGLSSFPLVMLQVTRFKCGEVCLGFAAHHHLVDGMSISFFFSSWARLARGLQLQAQPFVDRIDYLAPRNPPEVNFQHLEYDQPLPPILPEGLSGEIDATSRECMFTFTKHDIKTLNLQATYLDSEAQEANSHKLSTYEVLAGHVWRTACKARGLTGDQDVKLYIPTDGRSRLRDPTLPQGYLGNAIFFTACIAKAGDITCKPLRYAASKVQEALKRVQNTEYLRSAIDYLESEPDLSTLVRGAQHVTCPNLVINSWGKIPVDIADFGWGKPKFLVQGGVRHEGQAFLIPKQDQQGGFLLTIKLFNIHIPHFQDCLFDFESIPTD
>rna-XM_021992389.1
MSMLKAKANEECNVVENNISSFQALTGFLWKSITRIRNIHPDEETSCTLIINWRGRVIPPFSQEHFGNYLAVAQGRCKVGELVSRGLGWAALLVHQSVKAENDCKILEFLRNYSKAPYVEQAGKDSEFHNANSVSIAGSARFDMYGPEFGLGKPVAVLAGYGNKDDGKVTANPGCEGGGSVDLEICLRPQTMIGLESHEEFMSFAS
>rna-XM_021992376.1
MMNSHGEISQVKLISQSFVKPKIQVESSNQPHYLGMIDLAFLSLDQMQKGLLFHTKPENIQAHLLDKLKNSLSHSLVHFYPLAGQLATQKFHDEHAIWVYVDCNKGPGARLIHASVNNLKVSDLISSVDVHPIVRSFFDLGEKAVNHDGHTRPLLSIQVTELLDGVFIGFTMNHSIGDGTSLWNFISTLSEILLHLDGNNIGANDEKFNVSISHKPVFKPFYPDGYGPILKLPFLEPNEFITRNVDQGPLRERIFHFSPTSMSKLKVKANKECGLAYNHISSFQALTAFIWISITHARNLELGQETDVGLVANLRPKINPSISGDYFGGFVKRAQGSCKVGELLGQGLGWASILVHEVVMGLDDKAIRAFFKRFVEAPFVVQPSTANLYYKPNSVLIGGSTRFDMYGSEFGLGKAVGVLAGYASKEDGKVTANPGRQGDGSVDLEVCLRPEIMSSLELDEEFMSFVSAV
>rna-XM_021993934.1
MESSIVKKISECYVKPKNEVSESKTPYHVAPLDLIMLSYHYIQDGLLFMKPNSHLNGNNEFSIHSFLESLKQSLSDTLVHFYPLAGQLVVHFDEDQHQCRVFVDCNKGPGARFSHATALDLTVSDILSPNDDIPFVVRSFFDYNEEAVNYEGHSKPLLSVQVTQLIDGVFIACSINHAISDGTSYWHFWNVWSEIHRGSGNNVSISRLPIHNRWFPEGCVLPIPLPFTHPNQFVKKFEPPQLREKMFHFSPESIATLKTKANKESNSSDMISSFQALSALVWRSTVRANDLPHDQVIYNYMFANNRHRLNPPLPQNYFGTCIKGITTKTSAGELLDNNLGWAASLLHQSVLNLTDKVVRDFVKDWLKSPYCYHHTDLHDYNSIAMEHSPRFDMYGNDFGIGKPVAVRNGPGNKAVGVVFAYPGCEGNGSVDFEICLPPHSMKALETDDEFMAAVSLPK
>rna-XM_021995862.1
MERQLKLEVEIVSIETIKPSLPPPSHPKTYTLSLLDQIFPAFHIPVLLFYTSTQQRIDINTLKISLSQTLDRFYPLAGRFLDESTISCNNQGIPLIETKVDCHLKVFMSSPHKLNLLNKFLPSKDLQALGPRPVSELLPLAIQVNKFLCGGLVIGCYMLHKILDISSLGTFFKHWAACASNQHKDLLQLQPDFDAIVKAFPPLPMVEKVFVPEFCSKDTLNVLQHPTIVVKSFTFSTVSLTNLRAKAASELVPRPTRFEAVAGLLWEQTLAAAYTATGIHCLTGEVNNNNNNNNNNNNNNNNNNNNNNNNNNNNTVLSVTVNMRQWMSPPLPSGSMGNLVTNVMAKADKRSRFQELVKVIHSSLSYIKEKITKVYQSENGADILLSERKAESEGVMEYQEGLYRLSSWCRTGLNDADFGFGRPAWILPTDYSNPPPTLRNFMFLTDFADGIEAWLFLEEKEMQLLQSNPHFLAFASPN
>rna-XM_021983693.1
MEFWRVKEISKWFVKPKYSVSESKKPYHLGPLDLAMLSFHYIQNGLLFSKPSHLNGHEFSIDSFLESLKESLSHTLIHFYPLAGQLVIQVDEAKHECCVFVDCNKGPGAQFSHATALDITVADVLSPNNDIPLIVASFFYYNEPEVAVNYDGHFKPLLSVQVTELLDGVFIACSINHAIADGTAYWHFWNVWSEIHRAKDKGYLIPVSRLPIHNRWFPDGCVLPITLPFTRPEEFIRRFDTPQLREKVFHFSSDSINTIRAKANKESTGSSMMISTFQALSALVWRSIVRANCLPHDQILYNYMFASNRHRLNPPLPQNYFGTCIKGITTKTTAGELLKNNLGWAALLLHQSVVSLTDKAVREFVKEWLESPYCYHHRDLHDYNTVAMEHSPRFNMYGNEFGIGKPIAVRGGRGNKAVGVVNAYPGYQGTGSVDLEICLPPDSMRALEKF
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University