Enzyme
Specific for the penultimate step in salvianin biosynthesis. The enzyme also catalyses the malonylation of shisonin to malonylshisonin [cyanidin 3-O-(6''-O-p-coumaryl-beta-D-glucoside)-5-(6'''-O-malonyl-beta-D-glucoside)]. The compounds 4'''-demalonylsalvianin, salvianin, pelargonidin 3,5-diglucoside and delphinidin 3,5-diglucoside cannot act as substrates.
5MAT
Enzyme
Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase
Enzyme name
EC:2.3.1.172
Entry
Transferases;Acyltransferases;Transferring groups other than aminoacyl groups
Class
malonyl-CoA:pelargonidin-3-O-(6-caffeoyl-beta-D-glucoside)-5-O-beta-D-glucoside 6'''-O-malonyltransferase
Sysname
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside = CoA + 4'''-demalonylsalvianin [RN:R06799]
Reaction
CoA [CPD:C00010];4'''-demalonylsalvianin [CPD:C12641]
Product
Species information
Arabidopsis thaliana
Species name
Thale cress (Arabidopsis thaliana) is a species of eudicot in the family Brassicaceae (mustard family) that is widely used as an experimental model organism.
Species info
Gene sequence info
Arabidopsis_thaliana.5MAT.fa
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>rna-NM_113890.3
MALNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPKLEQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELRLETELRPLVPELQVSSDSASLLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKHGTTPQDFDLPTVLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAKEIDNDVVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANRPVRFMYAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEILSDSVRGLGSRNIESIWEVYEDGTKNMKLDTQNVTVTGSNQFGIYGSDFGWGRPVKTDVMSLYKNNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIYK
>rna-NM_113889.3
MTLHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSIILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYGQRPLSELHALVPKLPVSDDSATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKAWAQICKQELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLPSSKLGDDVVLATLVLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKARGGNKDRSVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISDLVKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSIDQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGLHS
>rna-NM_113885.2
MALKVTKISQVSPASNSSNDSANSMVLPLTFFDLRWLQFHPTERVIFYKLIKDSSLESFLSVILPKLELSLSIVLRHYLPLAGRLTWSSQDPKPSIIVSPNDYVSLTVAESDADFSRISGKGIRPESEIRSLVPELSLSCDSPSVLSLQVTLFPNQGFCIGIASHHSVMDGKTVVRFIKSWAHICKHGAMDLPEDLTPVLDRTVINVPASLDAKIIELLSYFSEVKDSFRSLKLLPPKEISPDLVRISLELTRENIEKLREQAKRESARSHHELHLSTFVVANAYLWTCLVKTRGGDENRPVRFMYAADFRNRLDPPVPEMYFGNCVFPIGCFGYKANVFLGEDGFVNMVEILSDSVRSIGLRKLETICELYINGTKSVKPGTQIGSIAGSNQFGLYGSDFGWGKPCNSEIASIDRNEAFSMSERRDEPGGVEIGLCLKKCEMDIFIYLFQNGLEMVL
>rna-NM_113880.2
MVNFNSAVNILEVVQVSPPSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELTRSSLISKLKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPATEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHNCKQEQEALPHDLVPSLDRIIVQDPTGLETKLLNRWISASNNKPSLKLFPSKIIGSDILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCVGFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLCPENIEKNMLLPFEAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKDASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE
>rna-NM_113894.1
MTKDESNGDNPDDGDAAKENITVGRLALQVVESEADFSYISSKELRLEAELRPLVPELQVSSDSASILSIQITFFPNQGFSIGTIVHHVVMDGKTASKFYKAWAHICKHGNIPQDFDLPMVLDRTVINVPAGLEPKLFQLLPYLSKEKVNARNLMLPPAKENINVVRVTLELSEANIKKLKEQAKNESTWSDLLLSTFVVTYEKSSGQSLFCKEEAKKSL
>rna-NM_123270.3
MVSSLNIIEVARVTPSNSDSAESLSLPLTYFDLIYYKLRAVERVIFYRITNVTRPFFDSVIVPNLKTSLSSCLSHYLPLAGKLIWEPLDHKPTIVYSQNDDVSFSVAETNADFSSLSGNEPFPSTELYPLVPALQSSDDSASIVSFQVTLFPNQGFCIGVSAHHAVLDGKTTTMFLKSWAHICKHQDFSLPQDLIPTYDRTVIKSPTDSENKVLNEWRSFTKILAGGKEPANPKSLKLNPSFEIGPDVVRYTLQLTREDIQTLRERLKREVSSSSSSTSSSKELRLSTFVIVYSYVLVCIIRARGGEPHRPVGYAFSVDCRSLMNPPTPNYFGNCIAGCSRMMLTAKMFMGEEGLLAAATMVSDSIEEWDESFAWKIPDFVAYATLPPETQLILVSGSNRFGVYELDFGWGRPDKVMVVSISPGNGISMAESRDQNGSVEIGFSLKKHEMDTLIDLLHCELTI
>rna-NM_125509.3
MALKVIKISRVSPATASVDPLIVPLSFFDLQWLKLNPTEQVFFYKLTESSSSRDVFYSSILPKLERSLSLILTHFRLFTGHLKWDSQDPKPHLVVLSGDTLSLTVAETDADFSRISGRGLRPELELRPLIPELPIYSDSGAVVSLQVTLFPKQGFCIGTTAHHVVLDGKTAEKFNKAWAHTCKHGTIPKILPTVLDRSVVNVPAGLEQKMLELLPYLTEDDKENGRTLKLPPVKEINAKDNVLRITIEISPENIEKLKERAKKESTRAELHLSTFVVTFAHVWTCMVKARSGDPNRPVRFMYAADFRNRLEPPVPVTYFGTCVLAMDFYKYKAKEFMGEDGFVNTVEILSDSVKRLASQGVESTWKVYEEGTKTMKWGTQLLVVNGSNQIGMYETDFGWGRPIHTETMSIYKNDEFSMSKRRDGIGGVEIGISLKKLEMDTFLSLFYKWIGN
>rna-NM_123267.6
MVNEEMESSLKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPFPTTELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWARTCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSLKLLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTCLIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAAARMVSDSVEALDENVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLEN
>rna-NM_123271.3
MNPSLNFIHVSRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIVPNLKSSLSSSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAESNSDFSLLTGKEPFSSTELHPLVPELQNSDDSAAVVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHLCKHQDSSLPDDLIPFYDRTVIKGPPEIDTKVLKIWHSIHKPKSLKLLPRPEIESDVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLIGSGGDDPNRPVGYRFAVDCRRLIDDPPIPLTYFGNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEELDSNVAWKIPELLETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQRGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK
MALNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPKLEQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELRLETELRPLVPELQVSSDSASLLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKHGTTPQDFDLPTVLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAKEIDNDVVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANRPVRFMYAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEILSDSVRGLGSRNIESIWEVYEDGTKNMKLDTQNVTVTGSNQFGIYGSDFGWGRPVKTDVMSLYKNNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIYK
>rna-NM_113889.3
MTLHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSIILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSHLSGYGQRPLSELHALVPKLPVSDDSATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSSTFIKAWAQICKQELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTSLPSSKLGDDVVLATLVLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKARGGNKDRSVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIISDLVKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVSIDQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGLHS
>rna-NM_113885.2
MALKVTKISQVSPASNSSNDSANSMVLPLTFFDLRWLQFHPTERVIFYKLIKDSSLESFLSVILPKLELSLSIVLRHYLPLAGRLTWSSQDPKPSIIVSPNDYVSLTVAESDADFSRISGKGIRPESEIRSLVPELSLSCDSPSVLSLQVTLFPNQGFCIGIASHHSVMDGKTVVRFIKSWAHICKHGAMDLPEDLTPVLDRTVINVPASLDAKIIELLSYFSEVKDSFRSLKLLPPKEISPDLVRISLELTRENIEKLREQAKRESARSHHELHLSTFVVANAYLWTCLVKTRGGDENRPVRFMYAADFRNRLDPPVPEMYFGNCVFPIGCFGYKANVFLGEDGFVNMVEILSDSVRSIGLRKLETICELYINGTKSVKPGTQIGSIAGSNQFGLYGSDFGWGKPCNSEIASIDRNEAFSMSERRDEPGGVEIGLCLKKCEMDIFIYLFQNGLEMVL
>rna-NM_113880.2
MVNFNSAVNILEVVQVSPPSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPELTRSSLISKLKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPRPATEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHNCKQEQEALPHDLVPSLDRIIVQDPTGLETKLLNRWISASNNKPSLKLFPSKIIGSDILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCVGFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLCPENIEKNMLLPFEAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKDASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE
>rna-NM_113894.1
MTKDESNGDNPDDGDAAKENITVGRLALQVVESEADFSYISSKELRLEAELRPLVPELQVSSDSASILSIQITFFPNQGFSIGTIVHHVVMDGKTASKFYKAWAHICKHGNIPQDFDLPMVLDRTVINVPAGLEPKLFQLLPYLSKEKVNARNLMLPPAKENINVVRVTLELSEANIKKLKEQAKNESTWSDLLLSTFVVTYEKSSGQSLFCKEEAKKSL
>rna-NM_123270.3
MVSSLNIIEVARVTPSNSDSAESLSLPLTYFDLIYYKLRAVERVIFYRITNVTRPFFDSVIVPNLKTSLSSCLSHYLPLAGKLIWEPLDHKPTIVYSQNDDVSFSVAETNADFSSLSGNEPFPSTELYPLVPALQSSDDSASIVSFQVTLFPNQGFCIGVSAHHAVLDGKTTTMFLKSWAHICKHQDFSLPQDLIPTYDRTVIKSPTDSENKVLNEWRSFTKILAGGKEPANPKSLKLNPSFEIGPDVVRYTLQLTREDIQTLRERLKREVSSSSSSTSSSKELRLSTFVIVYSYVLVCIIRARGGEPHRPVGYAFSVDCRSLMNPPTPNYFGNCIAGCSRMMLTAKMFMGEEGLLAAATMVSDSIEEWDESFAWKIPDFVAYATLPPETQLILVSGSNRFGVYELDFGWGRPDKVMVVSISPGNGISMAESRDQNGSVEIGFSLKKHEMDTLIDLLHCELTI
>rna-NM_125509.3
MALKVIKISRVSPATASVDPLIVPLSFFDLQWLKLNPTEQVFFYKLTESSSSRDVFYSSILPKLERSLSLILTHFRLFTGHLKWDSQDPKPHLVVLSGDTLSLTVAETDADFSRISGRGLRPELELRPLIPELPIYSDSGAVVSLQVTLFPKQGFCIGTTAHHVVLDGKTAEKFNKAWAHTCKHGTIPKILPTVLDRSVVNVPAGLEQKMLELLPYLTEDDKENGRTLKLPPVKEINAKDNVLRITIEISPENIEKLKERAKKESTRAELHLSTFVVTFAHVWTCMVKARSGDPNRPVRFMYAADFRNRLEPPVPVTYFGTCVLAMDFYKYKAKEFMGEDGFVNTVEILSDSVKRLASQGVESTWKVYEEGTKTMKWGTQLLVVNGSNQIGMYETDFGWGRPIHTETMSIYKNDEFSMSKRRDGIGGVEIGISLKKLEMDTFLSLFYKWIGN
>rna-NM_123267.6
MVNEEMESSLKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVPNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPFPTTELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWARTCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSLKLLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTCLIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAAARMVSDSVEALDENVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLEN
>rna-NM_123271.3
MNPSLNFIHVSRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIVPNLKSSLSSSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAESNSDFSLLTGKEPFSSTELHPLVPELQNSDDSAAVVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHLCKHQDSSLPDDLIPFYDRTVIKGPPEIDTKVLKIWHSIHKPKSLKLLPRPEIESDVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLIGSGGDDPNRPVGYRFAVDCRRLIDDPPIPLTYFGNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEELDSNVAWKIPELLETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQRGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University