Enzyme
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
D7OMT
Enzyme
Isoflavone 7-O-methyltransferase
Enzyme name
EC:2.1.1.150
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN:R07375]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];7-methoxyisoflavone [CPD:C15616]
Product
Species information
Spatholobus suberectus
Species name
Spatholobus suberectus is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Spatholobus_suberectus.D7OMT.fa
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>rna-gnl|WGS:QUWT|mrna.E2542_SST07942
MASINGRKASDLFQGQALLYRHLFAFVDSMYLKLIIELRIPDIIHNHGQPITLPELVSILQVPLAKVGQVHRLMRYLAHNGFFERVRIHDNGEEKEAYALTAASELLVKSNELSLAPMVECILDPTLSGSYHQLKKWVYEDDLTLFDVSLGCHFWDFLTKNPAYNESFNEAMASDSQLSNLALRDCKLVFEGLESIVDVGGGTGTTARIICEAFPNLKCIVFDRPQVVEHLSGTNNLTFVGGDMFKSIPKADAVLLKWILHNWTDKDCIKILKNCKEAILNNSKRGKVIIIDVVINEKQEEHKVTELKLLMDVNMACVNGQERSEEEWMKLFVEAGFQDYKISPLTGYLSLIEIYP
>rna-gnl|WGS:QUWT|mrna.E2542_SST16301
MASMNGYRATELFQAQAHLYRVMYSGPLTLASQYNPLPRSKRLLGFIHQNPTHMKTFNEAMESDSQVVRLALRDCKSVFEGLDSMVDVGDATGNTAKIICKAFPNLKYVVLDLPQVVTGLTGSKNLSFVGGNTFKSIPQADAILLKWALHNWSDDDCIKILKNCKDAISGKGKGGKVIIIDIVINE
>rna-gnl|WGS:QUWT|mrna.E2542_SST20700
MANANLMRSYSVLISDTDHCLSSMIKLLNNPYLVGSYHQLGKWASRENYPIIEIALGLKGHWDFIHQNLTYMRTFNEAMESDSLVVRLALTLRDSKFVF
>rna-gnl|WGS:QUWT|mrna.E2542_SST23496
MSLLVDLVTFKLEAKIFGHLRTMCLKWAVELGIPNIICNHEKPITLPELVSTLEIPLAKAGFV
>rna-gnl|WGS:QUWT|mrna.E2542_SST23497
MDSWGDPRVYEIALGTRFWGLIEKPPTYLSLFTEGMASDSQMVVLALKNCTLVFKELDSIMDVGGGTGTTARIICKAFPRLKCVVLDLPQVVANLTGSDNLSFVSGDMFKFVPKVDAILLKV
>rna-gnl|WGS:QUWT|mrna.E2542_SST23498
MASMNNQEAIELLEGQSLLYMQIFGHLRTMCLKWAVELGIPDIIYNHGKPITLPELVSTLEIPPAKAGFVQRLMRFLAHNGIFDIHERQEDHGLTYALTPASKLLVSASDHCLSPMVRCVTDPLLMSKYHHLGEWIRGEDLTVYETALGTSVWGLIEKQPTYLSLFAEGMASDSRMVDLALKNCTSVFEELDSIVDVGGGTGTTARIICKAFPRLKCVVLDLPQVVANLTGSDNLSFVGGDMFKFVPQADAVLLKWVLHDWNDENCIKILKKCKDSISSKGNRGKVIIIDTVIDEKLDERDMTQTKLCMDISMLAINGKERTEEEWKQLFIEAGFKQYKIFPVFGFRSLIEVYP
>rna-gnl|WGS:QUWT|mrna.E2542_SST27743
MSIKWAIDLGIPDIIHKHGQPITLPELVSALKVPQAKATCVQRLMRLLAHNDYFASSTLMMIAMKPTPQLGEWTCGEDLTIIETALGPGCYWDFIHQDAT
>rna-gnl|WGS:QUWT|mrna.E2542_SST27745
MASKNGYKASELLQAQAHLYRVLYSFLNPMSIKWAIDLGIPDIIHKHGQPITLPELVSALKVPQAKATCVQRLMRLLAHNDFFAIINTDDGSNEAYALTPPSQVLVNDTDHCLSSMVKLLNNPTLVGSYHQLGEWTCGEDLTIIETALGPGCYWDFIHQDATQMKMFNEAMESDSHIVRLALRDCKSVFEGLDSIVDVGGGTGNTAKIIGEAFPKLKYIVLDLPQVVAGITGSNNLSFVGGNMFKSVPQADAVLLKWVLHNWSDDDCIKILKNCKEAIAGKGEGGKVIIIDIVINEKQDEHKMTEMKLLFDIVMMTYHNGKERSEKDWKRLFVEAGFKSYKLFPAFGFRSLIELYP
MASINGRKASDLFQGQALLYRHLFAFVDSMYLKLIIELRIPDIIHNHGQPITLPELVSILQVPLAKVGQVHRLMRYLAHNGFFERVRIHDNGEEKEAYALTAASELLVKSNELSLAPMVECILDPTLSGSYHQLKKWVYEDDLTLFDVSLGCHFWDFLTKNPAYNESFNEAMASDSQLSNLALRDCKLVFEGLESIVDVGGGTGTTARIICEAFPNLKCIVFDRPQVVEHLSGTNNLTFVGGDMFKSIPKADAVLLKWILHNWTDKDCIKILKNCKEAILNNSKRGKVIIIDVVINEKQEEHKVTELKLLMDVNMACVNGQERSEEEWMKLFVEAGFQDYKISPLTGYLSLIEIYP
>rna-gnl|WGS:QUWT|mrna.E2542_SST16301
MASMNGYRATELFQAQAHLYRVMYSGPLTLASQYNPLPRSKRLLGFIHQNPTHMKTFNEAMESDSQVVRLALRDCKSVFEGLDSMVDVGDATGNTAKIICKAFPNLKYVVLDLPQVVTGLTGSKNLSFVGGNTFKSIPQADAILLKWALHNWSDDDCIKILKNCKDAISGKGKGGKVIIIDIVINE
>rna-gnl|WGS:QUWT|mrna.E2542_SST20700
MANANLMRSYSVLISDTDHCLSSMIKLLNNPYLVGSYHQLGKWASRENYPIIEIALGLKGHWDFIHQNLTYMRTFNEAMESDSLVVRLALTLRDSKFVF
>rna-gnl|WGS:QUWT|mrna.E2542_SST23496
MSLLVDLVTFKLEAKIFGHLRTMCLKWAVELGIPNIICNHEKPITLPELVSTLEIPLAKAGFV
>rna-gnl|WGS:QUWT|mrna.E2542_SST23497
MDSWGDPRVYEIALGTRFWGLIEKPPTYLSLFTEGMASDSQMVVLALKNCTLVFKELDSIMDVGGGTGTTARIICKAFPRLKCVVLDLPQVVANLTGSDNLSFVSGDMFKFVPKVDAILLKV
>rna-gnl|WGS:QUWT|mrna.E2542_SST23498
MASMNNQEAIELLEGQSLLYMQIFGHLRTMCLKWAVELGIPDIIYNHGKPITLPELVSTLEIPPAKAGFVQRLMRFLAHNGIFDIHERQEDHGLTYALTPASKLLVSASDHCLSPMVRCVTDPLLMSKYHHLGEWIRGEDLTVYETALGTSVWGLIEKQPTYLSLFAEGMASDSRMVDLALKNCTSVFEELDSIVDVGGGTGTTARIICKAFPRLKCVVLDLPQVVANLTGSDNLSFVGGDMFKFVPQADAVLLKWVLHDWNDENCIKILKKCKDSISSKGNRGKVIIIDTVIDEKLDERDMTQTKLCMDISMLAINGKERTEEEWKQLFIEAGFKQYKIFPVFGFRSLIEVYP
>rna-gnl|WGS:QUWT|mrna.E2542_SST27743
MSIKWAIDLGIPDIIHKHGQPITLPELVSALKVPQAKATCVQRLMRLLAHNDYFASSTLMMIAMKPTPQLGEWTCGEDLTIIETALGPGCYWDFIHQDAT
>rna-gnl|WGS:QUWT|mrna.E2542_SST27745
MASKNGYKASELLQAQAHLYRVLYSFLNPMSIKWAIDLGIPDIIHKHGQPITLPELVSALKVPQAKATCVQRLMRLLAHNDFFAIINTDDGSNEAYALTPPSQVLVNDTDHCLSSMVKLLNNPTLVGSYHQLGEWTCGEDLTIIETALGPGCYWDFIHQDATQMKMFNEAMESDSHIVRLALRDCKSVFEGLDSIVDVGGGTGNTAKIIGEAFPKLKYIVLDLPQVVAGITGSNNLSFVGGNMFKSVPQADAVLLKWVLHNWSDDDCIKILKNCKEAIAGKGEGGKVIIIDIVINEKQDEHKMTEMKLLFDIVMMTYHNGKERSEKDWKRLFVEAGFKSYKLFPAFGFRSLIELYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University